{"id":"https://openalex.org/W2298205297","doi":"https://doi.org/10.1093/nar/gkw138","title":"Kinetics of the CRISPR-Cas9 effector complex assembly and the role of 3\u2032-terminal segment of guide RNA","display_name":"Kinetics of the CRISPR-Cas9 effector complex assembly and the role of 3\u2032-terminal segment of guide RNA","publication_year":2016,"publication_date":"2016-03-03","ids":{"openalex":"https://openalex.org/W2298205297","doi":"https://doi.org/10.1093/nar/gkw138","mag":"2298205297","pmid":"https://pubmed.ncbi.nlm.nih.gov/26945042","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/4824121"},"language":"en","primary_location":{"id":"doi:10.1093/nar/gkw138","doi":"https://doi.org/10.1093/nar/gkw138","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gkw138","pdf_url":"https://academic.oup.com/nar/article-pdf/44/6/2837/25346464/gkw138.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://academic.oup.com/nar/article-pdf/44/6/2837/25346464/gkw138.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5006155058","display_name":"Vladimir M. Mekler","orcid":"https://orcid.org/0000-0002-8923-7615","observed_orcids":["https://orcid.org/0000-0002-8923-7615"]},"institutions":[{"id":"https://openalex.org/I102322142","display_name":"Rutgers, The State University of New Jersey","ror":"https://ror.org/05vt9qd57","country_code":"US","type":"education","lineage":["https://openalex.org/I102322142"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Vladimir Mekler","raw_affiliation_strings":["Waksman Institute of Microbiology and Department of Molecular Biology and Biochemistry, Rutgers, State University of New Jersey, Piscataway, NJ 08854, USA mekler@waksman.rutgers.edu","Waksman Institute of Microbiology and Department of Molecular Biology and Biochemistry, Rutgers, State University of New Jersey, Piscataway, NJ 08854, USA,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Waksman Institute of Microbiology and Department of Molecular Biology and Biochemistry, Rutgers, State University of New Jersey, Piscataway, NJ 08854, USA mekler@waksman.rutgers.edu","institution_ids":["https://openalex.org/I102322142"]},{"raw_affiliation_string":"Waksman Institute of Microbiology and Department of Molecular Biology and Biochemistry, Rutgers, State University of New Jersey, Piscataway, NJ 08854, USA,","institution_ids":["https://openalex.org/I102322142"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5029887812","display_name":"\u041c\u0438\u043d\u0430\u0445\u0438\u043d \u041b\u0435\u043e\u043d\u0438\u0434 \u0421\u0442\u0430\u043d\u0438\u0441\u043b\u0430\u0432\u043e\u0432\u0438\u0447","orcid":"https://orcid.org/0000-0003-0301-7054","observed_orcids":["https://orcid.org/0000-0003-0301-7054"]},"institutions":[{"id":"https://openalex.org/I102322142","display_name":"Rutgers, The State University of New Jersey","ror":"https://ror.org/05vt9qd57","country_code":"US","type":"education","lineage":["https://openalex.org/I102322142"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Leonid Minakhin","raw_affiliation_strings":["Waksman Institute of Microbiology and Department of Molecular Biology and Biochemistry, Rutgers, State University of New Jersey, Piscataway, NJ 08854, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Waksman Institute of Microbiology and Department of Molecular Biology and Biochemistry, Rutgers, State University of New Jersey, Piscataway, NJ 08854, USA","institution_ids":["https://openalex.org/I102322142"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023634828","display_name":"Ekaterina Semenova","orcid":"https://orcid.org/0000-0001-6816-8879","observed_orcids":["https://orcid.org/0000-0001-6816-8879"]},"institutions":[{"id":"https://openalex.org/I102322142","display_name":"Rutgers, The State University of New Jersey","ror":"https://ror.org/05vt9qd57","country_code":"US","type":"education","lineage":["https://openalex.org/I102322142"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ekaterina Semenova","raw_affiliation_strings":["Waksman Institute of Microbiology and Department of Molecular Biology and Biochemistry, Rutgers, State University of New Jersey, Piscataway, NJ 08854, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Waksman Institute of Microbiology and Department of Molecular Biology and Biochemistry, Rutgers, State University of New Jersey, Piscataway, NJ 08854, USA","institution_ids":["https://openalex.org/I102322142"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5046213084","display_name":"Konstantin D. Kuznedelov","orcid":"https://orcid.org/0000-0002-9040-3895","observed_orcids":["https://orcid.org/0000-0002-9040-3895"]},"institutions":[{"id":"https://openalex.org/I102322142","display_name":"Rutgers, The State University of New Jersey","ror":"https://ror.org/05vt9qd57","country_code":"US","type":"education","lineage":["https://openalex.org/I102322142"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Konstantin Kuznedelov","raw_affiliation_strings":["Waksman Institute of Microbiology and Department of Molecular Biology and Biochemistry, Rutgers, State University of New Jersey, Piscataway, NJ 08854, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Waksman Institute of Microbiology and Department of Molecular Biology and Biochemistry, Rutgers, State University of New Jersey, Piscataway, NJ 08854, USA","institution_ids":["https://openalex.org/I102322142"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5056880626","display_name":"Konstantin Severinov","orcid":"https://orcid.org/0000-0001-9706-450X","observed_orcids":["https://orcid.org/0000-0001-9706-450X"]},"institutions":[{"id":"https://openalex.org/I102322142","display_name":"Rutgers, The State University of New Jersey","ror":"https://ror.org/05vt9qd57","country_code":"US","type":"education","lineage":["https://openalex.org/I102322142"]},{"id":"https://openalex.org/I1313323035","display_name":"Russian Academy of Sciences","ror":"https://ror.org/05qrfxd25","country_code":"RU","type":"government","lineage":["https://openalex.org/I1313323035"]},{"id":"https://openalex.org/I212220629","display_name":"Peter the Great St. Petersburg Polytechnic University","ror":"https://ror.org/02x91aj62","country_code":"RU","type":"education","lineage":["https://openalex.org/I212220629"]}],"countries":["RU","US"],"is_corresponding":false,"raw_author_name":"Konstantin Severinov","raw_affiliation_strings":["Waksman Institute of Microbiology and Department of Molecular Biology and Biochemistry, Rutgers, State University of New Jersey, Piscataway, NJ 08854, USA Institutes of Molecular Genetics and Gene Biology, Russian Academy of Sciences, Moscow 119334, Russia Peter the Great State Polytechnical Institute, St. Petersburg, 195251, Russia severik@waksman.rutgers.edu","Institutes of Molecular Genetics and Gene Biology, Russian Academy of Sciences, Moscow 119334, Russia and","Peter the Great State Polytechnical Institute, St. Petersburg, 195251, Russia","Waksman Institute of Microbiology and Department of Molecular Biology and Biochemistry, Rutgers, State University of New Jersey, Piscataway, NJ 08854, USA,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Waksman Institute of Microbiology and Department of Molecular Biology and Biochemistry, Rutgers, State University of New Jersey, Piscataway, NJ 08854, USA Institutes of Molecular Genetics and Gene Biology, Russian Academy of Sciences, Moscow 119334, Russia Peter the Great State Polytechnical Institute, St. Petersburg, 195251, Russia severik@waksman.rutgers.edu","institution_ids":["https://openalex.org/I102322142","https://openalex.org/I1313323035"]},{"raw_affiliation_string":"Institutes of Molecular Genetics and Gene Biology, Russian Academy of Sciences, Moscow 119334, Russia and","institution_ids":["https://openalex.org/I1313323035"]},{"raw_affiliation_string":"Peter the Great State Polytechnical Institute, St. Petersburg, 195251, Russia","institution_ids":["https://openalex.org/I212220629"]},{"raw_affiliation_string":"Waksman Institute of Microbiology and Department of Molecular Biology and Biochemistry, Rutgers, State University of New Jersey, Piscataway, NJ 08854, USA,","institution_ids":["https://openalex.org/I102322142"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5006155058"],"corresponding_institution_ids":["https://openalex.org/I102322142"],"apc_list":{"value":3992,"currency":"USD","value_usd":3992},"apc_paid":{"value":3992,"currency":"USD","value_usd":3992},"fwci":6.0617,"has_fulltext":true,"cited_by_count":89,"citation_normalized_percentile":{"value":0.96991801,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"44","issue":"6","first_page":"2837","last_page":"2845"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12610","display_name":"RNA regulation and disease","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/crispr-cas9","display_name":"CRISPR/Cas9","score":0.9980000257492065},{"id":"https://openalex.org/keywords/single-guide-rna","display_name":"single guide RNA","score":0.8830000162124634},{"id":"https://openalex.org/keywords/assembly-kinetics","display_name":"assembly kinetics","score":0.7900000214576721},{"id":"https://openalex.org/keywords/genome-editing","display_name":"genome editing","score":0.5809999704360962},{"id":"https://openalex.org/keywords/stem-loop-structure","display_name":"stem-loop structure","score":0.45100000500679016}],"concepts":[{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.9032683372497559},{"id":"https://openalex.org/C132455925","wikidata":"https://www.wikidata.org/wiki/Q16965677","display_name":"Cas9","level":4,"score":0.8570417165756226},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8216257095336914},{"id":"https://openalex.org/C97702854","wikidata":"https://www.wikidata.org/wiki/Q4039747","display_name":"Guide RNA","level":5,"score":0.8115564584732056},{"id":"https://openalex.org/C51785407","wikidata":"https://www.wikidata.org/wiki/Q2627568","display_name":"Effector","level":2,"score":0.7713342308998108},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.6960809826850891},{"id":"https://openalex.org/C144501496","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome editing","level":4,"score":0.5666834712028503},{"id":"https://openalex.org/C189819185","wikidata":"https://www.wikidata.org/wiki/Q384283","display_name":"Subgenomic mRNA","level":4,"score":0.4781378507614136},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4524078667163849},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.42344290018081665},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3740685284137726},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.19659534096717834}],"mesh":[{"descriptor_ui":"D000076987","descriptor_name":"CRISPR-Associated Protein 9","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000094704","descriptor_name":"RNA, Guide, CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000094704","descriptor_name":"RNA, Guide, CRISPR-Cas Systems","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000094704","descriptor_name":"RNA, Guide, CRISPR-Cas Systems","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001667","descriptor_name":"Binding, Competitive","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001681","descriptor_name":"Biological Assay","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D004720","descriptor_name":"Endonucleases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004720","descriptor_name":"Endonucleases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009841","descriptor_name":"Oligonucleotides","qualifier_ui":"Q000138","qualifier_name":"chemical synthesis","is_major_topic":false},{"descriptor_ui":"D009841","descriptor_name":"Oligonucleotides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013297","descriptor_name":"Streptococcus pyogenes","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D013297","descriptor_name":"Streptococcus pyogenes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D020029","descriptor_name":"Base Pairing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D031541","descriptor_name":"Fluorescence Resonance Energy Transfer","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D064112","descriptor_name":"Clustered Regularly Interspaced Short Palindromic Repeats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":5,"locations":[{"id":"doi:10.1093/nar/gkw138","doi":"https://doi.org/10.1093/nar/gkw138","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gkw138","pdf_url":"https://academic.oup.com/nar/article-pdf/44/6/2837/25346464/gkw138.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:3795100","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4824121","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:4824121","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4824121","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Res","raw_type":"Text"},{"id":"pmid:26945042","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/26945042","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic acids research","raw_type":"Journal Article"},{"id":"pmh:oai:open-archive.highwire.org:nar:44/6/2837","doi":null,"is_oa":false,"landing_page_url":"http://nar.oxfordjournals.org/cgi/content/short/44/6/2837","pdf_url":null,"source":{"id":"https://openalex.org/S4406923041","display_name":"HighWire Press Open Archive","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"TEXT"}],"best_oa_location":{"id":"doi:10.1093/nar/gkw138","doi":"https://doi.org/10.1093/nar/gkw138","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gkw138","pdf_url":"https://academic.oup.com/nar/article-pdf/44/6/2837/25346464/gkw138.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1678376580","display_name":null,"funder_award_id":"GM10407","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G3397940999","display_name":null,"funder_award_id":"GM10407","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G3578279686","display_name":null,"funder_award_id":"14\u201014\u201000988","funder_id":"https://openalex.org/F4320324099","funder_display_name":"Russian Science Foundation"}],"funders":[{"id":"https://openalex.org/F4320324099","display_name":"Russian Science Foundation","ror":"https://ror.org/03y2gwe85"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2298205297.pdf","grobid_xml":"https://content.openalex.org/works/W2298205297.grobid-xml"},"referenced_works_count":44,"referenced_works":["https://openalex.org/W1491459594","https://openalex.org/W1506494596","https://openalex.org/W1594078235","https://openalex.org/W1915704240","https://openalex.org/W1968342623","https://openalex.org/W1970196890","https://openalex.org/W1976391205","https://openalex.org/W1976404496","https://openalex.org/W1978094899","https://openalex.org/W1981160614","https://openalex.org/W2009966858","https://openalex.org/W2010986636","https://openalex.org/W2021134519","https://openalex.org/W2026520402","https://openalex.org/W2036176224","https://openalex.org/W2039705224","https://openalex.org/W2041131616","https://openalex.org/W2045435533","https://openalex.org/W2052192736","https://openalex.org/W2062008243","https://openalex.org/W2064815984","https://openalex.org/W2068427957","https://openalex.org/W2077659966","https://openalex.org/W2101749257","https://openalex.org/W2104820165","https://openalex.org/W2106973449","https://openalex.org/W2107959600","https://openalex.org/W2108038398","https://openalex.org/W2112295554","https://openalex.org/W2114163400","https://openalex.org/W2116534141","https://openalex.org/W2118292710","https://openalex.org/W2120503144","https://openalex.org/W2149402050","https://openalex.org/W2153344788","https://openalex.org/W2154219213","https://openalex.org/W2156319424","https://openalex.org/W2158312398","https://openalex.org/W2164695230","https://openalex.org/W2167380395","https://openalex.org/W2169244146","https://openalex.org/W2188223075","https://openalex.org/W2203807603","https://openalex.org/W2484307026"],"related_works":["https://openalex.org/W4312109996","https://openalex.org/W6889166067","https://openalex.org/W2478823450","https://openalex.org/W3200788662","https://openalex.org/W4405221199","https://openalex.org/W2548710592","https://openalex.org/W6892354429","https://openalex.org/W2513896518","https://openalex.org/W2770138328","https://openalex.org/W4200428983"],"abstract_inverted_index":{"CRISPR-Cas9":[0,36],"is":[1,78],"widely":[2],"applied":[3],"for":[4,33,128,182],"genome":[5],"engineering":[6],"in":[7,115,171],"various":[8],"organisms.":[9],"The":[10],"assembly":[11,52,76],"of":[12,35,50,56,83,86,110,142,179],"single":[13],"guide":[14],"RNA":[15,88,126,167],"(sgRNA)":[16],"with":[17,53,145],"the":[18,23,48,68,75,81,93,108,129,155,160,175,183],"Cas9":[19,51,63,114,143],"protein":[20],"may":[21,173],"limit":[22],"Cas9/sgRNA":[24,94],"effector":[25,69],"complex":[26,37,70,95],"function.":[27],"We":[28,60],"developed":[29],"a":[30,54],"FRET-based":[31],"assay":[32,45],"detection":[34],"binding":[38,130],"to":[39,46,80,131,162,188],"its":[40],"targets":[41],"and":[42,64,72,124,168],"used":[43],"this":[44],"investigate":[47],"kinetics":[49],"set":[55],"structurally":[57],"distinct":[58],"sgRNAs.":[59],"find":[61],"that":[62,107,154,177],"isolated":[65],"sgRNAs":[66,146,180],"form":[67],"efficiently":[71],"rapidly.":[73],"Yet,":[74],"process":[77],"sensitive":[79],"presence":[82],"moderate":[84],"concentrations":[85],"non-specific":[87,166],"competitors,":[89],"which":[90],"considerably":[91],"delay":[92],"formation,":[96],"while":[97],"not":[98],"significantly":[99],"affecting":[100],"already":[101],"formed":[102],"complexes.":[103],"This":[104,151],"observation":[105],"suggests":[106],"rate":[109],"sgRNA":[111,123,159],"loading":[112],"into":[113],"cells":[116],"can":[117],"be":[118],"determined":[119],"by":[120],"competition":[121,164],"between":[122],"intracellular":[125],"molecules":[127],"Cas9.":[132],"Non-specific":[133],"RNAs":[134],"exerted":[135],"particularly":[136],"large":[137],"inhibitory":[138],"effects":[139],"on":[140],"formation":[141],"complexes":[144],"bearing":[147],"shortened":[148],"3'-terminal":[149,156,184],"segments.":[150],"result":[152],"implies":[153],"segment":[157],"confers":[158],"ability":[161],"withstand":[163],"from":[165],"at":[169],"least":[170],"part":[172],"explain":[174],"fact":[176],"use":[178],"truncated":[181],"stem":[185],"loops":[186],"leads":[187],"reduced":[189],"activity":[190],"during":[191],"genomic":[192],"editing.":[193]},"counts_by_year":[{"year":2026,"cited_by_count":6},{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":10},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":11},{"year":2021,"cited_by_count":6},{"year":2020,"cited_by_count":11},{"year":2019,"cited_by_count":10},{"year":2018,"cited_by_count":13},{"year":2017,"cited_by_count":6},{"year":2016,"cited_by_count":6},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
