{"id":"https://openalex.org/W2297901518","doi":"https://doi.org/10.1186/s12575-016-0038-x","title":"A Modified Coupled Spectrophotometric Method to Detect 2-5 Oligoadenylate Synthetase Activity in Prostate Cell Lines","display_name":"A Modified Coupled Spectrophotometric Method to Detect 2-5 Oligoadenylate Synthetase Activity in Prostate Cell Lines","publication_year":2016,"publication_date":"2016-03-17","ids":{"openalex":"https://openalex.org/W2297901518","doi":"https://doi.org/10.1186/s12575-016-0038-x","mag":"2297901518","pmid":"https://pubmed.ncbi.nlm.nih.gov/26997919","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/4797170"},"language":"en","primary_location":{"id":"doi:10.1186/s12575-016-0038-x","doi":"https://doi.org/10.1186/s12575-016-0038-x","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s12575-016-0038-x","pdf_url":"https://biologicalproceduresonline.biomedcentral.com/track/pdf/10.1186/s12575-016-0038-x","source":{"id":"https://openalex.org/S93143966","display_name":"Biological Procedures Online","issn_l":"1480-9222","issn":["1480-9222"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","norway-1","oarl","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biological Procedures Online","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://biologicalproceduresonline.biomedcentral.com/track/pdf/10.1186/s12575-016-0038-x","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5061811652","display_name":"Sushma M. Bhosle","orcid":"https://orcid.org/0000-0003-4356-4239","observed_orcids":["https://orcid.org/0000-0003-4356-4239"]},"institutions":[{"id":"https://openalex.org/I52064589","display_name":"Clark Atlanta University","ror":"https://ror.org/0397tsa92","country_code":"US","type":"education","lineage":["https://openalex.org/I52064589"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sushma M. Bhosle","raw_affiliation_strings":["Department of Biological Sciences, Centre for Cancer Research and Therapeutic Development, Clark Atlanta University, Atlanta, GA, 30314, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Sciences, Centre for Cancer Research and Therapeutic Development, Clark Atlanta University, Atlanta, GA, 30314, USA","institution_ids":["https://openalex.org/I52064589"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5064347762","display_name":"Aisha Hunt","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I52064589","display_name":"Clark Atlanta University","ror":"https://ror.org/0397tsa92","country_code":"US","type":"education","lineage":["https://openalex.org/I52064589"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Aisha Hunt","raw_affiliation_strings":["Department of Biological Sciences, Centre for Cancer Research and Therapeutic Development, Clark Atlanta University, Atlanta, GA, 30314, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Sciences, Centre for Cancer Research and Therapeutic Development, Clark Atlanta University, Atlanta, GA, 30314, USA","institution_ids":["https://openalex.org/I52064589"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5072992833","display_name":"Jaideep Chaudhary","orcid":"https://orcid.org/0000-0002-8793-4329","observed_orcids":["https://orcid.org/0000-0002-8793-4329"]},"institutions":[{"id":"https://openalex.org/I52064589","display_name":"Clark Atlanta University","ror":"https://ror.org/0397tsa92","country_code":"US","type":"education","lineage":["https://openalex.org/I52064589"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Jaideep Chaudhary","raw_affiliation_strings":["Department of Biological Sciences, Centre for Cancer Research and Therapeutic Development, Clark Atlanta University, Atlanta, GA, 30314, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Sciences, Centre for Cancer Research and Therapeutic Development, Clark Atlanta University, Atlanta, GA, 30314, USA","institution_ids":["https://openalex.org/I52064589"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5072992833"],"corresponding_institution_ids":["https://openalex.org/I52064589"],"apc_list":{"value":3490,"currency":"USD","value_usd":3490},"apc_paid":{"value":3490,"currency":"USD","value_usd":3490},"fwci":0.2005,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":{"value":0.62274253,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"18","issue":"1","first_page":"9","last_page":"9"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11668","display_name":"interferon and immune responses","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11668","display_name":"interferon and immune responses","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12610","display_name":"RNA regulation and disease","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/2-5-oligoadenylate-synthetase","display_name":"2',5'-oligoadenylate synthetase","score":0.9850000143051147},{"id":"https://openalex.org/keywords/prostate-cancer-cell-lines","display_name":"prostate cancer cell lines","score":0.9449999928474426},{"id":"https://openalex.org/keywords/purine-nucleoside-phosphorylase","display_name":"purine nucleoside phosphorylase","score":0.9610000252723694},{"id":"https://openalex.org/keywords/pyrophosphate-detection","display_name":"pyrophosphate detection","score":0.6930000185966492},{"id":"https://openalex.org/keywords/innate-immunity","display_name":"innate immunity","score":0.5559999942779541},{"id":"https://openalex.org/keywords/rnase-l","display_name":"RNase L","score":0.4830000102519989}],"concepts":[{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.6197571754455566},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.5415525436401367},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.481865257024765},{"id":"https://openalex.org/C53345823","wikidata":"https://www.wikidata.org/wiki/Q5531554","display_name":"Gene isoform","level":3,"score":0.45309382677078247},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.43419185280799866},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4299252927303314},{"id":"https://openalex.org/C28212737","wikidata":"https://www.wikidata.org/wiki/Q5381543","display_name":"Enzyme assay","level":3,"score":0.41830819845199585},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.40385890007019043},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.09773498773574829},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[],"locations_count":4,"locations":[{"id":"doi:10.1186/s12575-016-0038-x","doi":"https://doi.org/10.1186/s12575-016-0038-x","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s12575-016-0038-x","pdf_url":"https://biologicalproceduresonline.biomedcentral.com/track/pdf/10.1186/s12575-016-0038-x","source":{"id":"https://openalex.org/S93143966","display_name":"Biological Procedures Online","issn_l":"1480-9222","issn":["1480-9222"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","norway-1","oarl","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biological Procedures Online","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:4797170","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4797170","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biol Proced Online","raw_type":"Text"},{"id":"pmid:26997919","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/26997919","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biological procedures online","raw_type":"Journal Article"},{"id":"pmh:oai:digitalcommons.auctr.edu:caupubs-1021","doi":null,"is_oa":false,"landing_page_url":"http://digitalcommons.auctr.edu/cgi/viewcontent.cgi?article=1021&context=caupubs","pdf_url":null,"source":{"id":"https://openalex.org/S4377196624","display_name":"Atlanta University Center Robert W. Woodruff Library (Atlanta University Center Robert W. Woodruff Library)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210116871","host_organization_name":"Atlanta University Center Robert W. Woodruff Library","host_organization_lineage":["https://openalex.org/I4210116871"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Clark Atlanta University Faculty Publications","raw_type":"text"}],"best_oa_location":{"id":"doi:10.1186/s12575-016-0038-x","doi":"https://doi.org/10.1186/s12575-016-0038-x","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s12575-016-0038-x","pdf_url":"https://biologicalproceduresonline.biomedcentral.com/track/pdf/10.1186/s12575-016-0038-x","source":{"id":"https://openalex.org/S93143966","display_name":"Biological Procedures Online","issn_l":"1480-9222","issn":["1480-9222"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","norway-1","oarl","poland-100","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biological Procedures Online","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.8048999905586243,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G7546212254","display_name":"CNTR OF EXCELLENCE FOR PROSTATE CANCER RESEARCH EDUCATION AND COMM SERVICE","funder_award_id":"P20MD002285","funder_id":"https://openalex.org/F4320337534","funder_display_name":"National Institute on Minority Health and Health Disparities"},{"id":"https://openalex.org/G7737758266","display_name":"CANCER RESEARCH INFRASTRUCTURE DEVELOPMENT","funder_award_id":"G12MD007590","funder_id":"https://openalex.org/F4320337534","funder_display_name":"National Institute on Minority Health and Health Disparities"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337534","display_name":"National Institute on Minority Health and Health Disparities","ror":"https://ror.org/0493hgw16"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2297901518.pdf","grobid_xml":"https://content.openalex.org/works/W2297901518.grobid-xml"},"referenced_works_count":38,"referenced_works":["https://openalex.org/W1525883576","https://openalex.org/W1593536157","https://openalex.org/W1970677867","https://openalex.org/W2010018654","https://openalex.org/W2010532053","https://openalex.org/W2016790921","https://openalex.org/W2022056950","https://openalex.org/W2027022722","https://openalex.org/W2029584311","https://openalex.org/W2032656149","https://openalex.org/W2035117693","https://openalex.org/W2039361749","https://openalex.org/W2048352784","https://openalex.org/W2050575361","https://openalex.org/W2052503751","https://openalex.org/W2055749608","https://openalex.org/W2056133364","https://openalex.org/W2056940370","https://openalex.org/W2057484128","https://openalex.org/W2060325852","https://openalex.org/W2061925892","https://openalex.org/W2063322107","https://openalex.org/W2067171614","https://openalex.org/W2081893364","https://openalex.org/W2087004183","https://openalex.org/W2087417534","https://openalex.org/W2094191346","https://openalex.org/W2096709898","https://openalex.org/W2099336504","https://openalex.org/W2116239878","https://openalex.org/W2122994357","https://openalex.org/W2125238675","https://openalex.org/W2126916706","https://openalex.org/W2127304974","https://openalex.org/W2129499324","https://openalex.org/W2138316859","https://openalex.org/W2155246535","https://openalex.org/W2201257844"],"related_works":["https://openalex.org/W1500943849","https://openalex.org/W2128702837","https://openalex.org/W2252684662","https://openalex.org/W2938461592","https://openalex.org/W2125238675","https://openalex.org/W7009707487","https://openalex.org/W1972327398","https://openalex.org/W7095378284","https://openalex.org/W1974032862","https://openalex.org/W2128966128"],"abstract_inverted_index":{"BACKGROUND:":[0],"2'-5'":[1],"oligoadenylate":[2,148],"synthetases":[3],"(OAS)":[4,150],"are":[5,27,108],"interferon":[6],"inducible":[7],"enzymes":[8,26,74,84,315],"that":[9,47,104],"polymerizes":[10],"ATP":[11],"to":[12,145,241],"2'-5'-linked":[13],"oligomers":[14],"of":[15,20,55,119,191,194,231,264,277,287,313],"adenylate":[16],"(2-5As).":[17],"As":[18],"part":[19],"the":[21,198,219,223,232,265,317],"innate":[22],"immune":[23],"response,":[24],"these":[25],"activated":[28,178,182],"by":[29,222],"viral":[30,112],"double":[31,38],"stranded":[32,39,50],"RNA":[33],"or":[34],"mRNAs":[35],"with":[36,65,111,123,229,304],"significant":[37,88],"structure.":[40],"The":[41,72,161,181,262,274,296],"2-5As":[42],"in":[43,58,90,128,133,153,203,211,280,289,311],"turn":[44],"activate":[45],"RNaseL":[46],"degrade":[48],"single":[49],"RNAs.":[51],"Three":[52],"distinct":[53],"forms":[54],"OAS":[56,73,83,106,120,162,183,201,224,288,301,314],"exist":[57],"human":[59],"cells":[60,129],"(OAS1,":[61],"2":[62],"and":[63,75,98,115,247,306,319],"3)":[64],"each":[66],"form":[67],"having":[68],"multiple":[69,92],"spliced":[70,77],"variants.":[71],"their":[76],"variants":[78],"have":[79],"different":[80],"enzyme":[81,121,151,163,184,302],"activities.":[82],"also":[85,109],"play":[86],"a":[87,124,140,158,237,242,250,257],"role":[89],"regulating":[91],"cellular":[93],"processes":[94],"such":[95],"as":[96,157],"proliferation":[97],"apoptosis.":[99],"Moreover,":[100],"Single":[101],"nucleotide":[102],"polymorphisms":[103],"alter":[105],"activity":[107,122,152,286,303],"associated":[110],"infection,":[113],"diabetes":[114],"cancer.":[116],"Thus":[117],"detection":[118,312],"simple":[125,298],"spectrophotometric":[126,143],"method":[127,299],"will":[130],"be":[131],"important":[132],"clinical":[134],"research.":[135],"RESULTS:":[136],"Here":[137],"we":[138],"propose":[139],"modified":[141,297],"coupled":[142,228],"assay":[144,218],"detect":[146],"2-5":[147],"synthetase":[149],"prostate":[154,165,213,291],"cell":[155,167,207,214,282,293],"lines":[156],"model":[159],"system.":[160],"from":[164,186,205],"cancer":[166,292],"lysates":[168],"was":[169],"purified":[170],"using":[171,256],"Polyinosinic:":[172],"polycytidylic":[173],"acid":[174],"(poly":[175],"I:C)":[176],"bound":[177],"sepharose":[179],"beads.":[180],"eluted":[185],"Sepharose":[187],"beads":[188],"showed":[189],"expression":[190],"p46":[192],"isoform":[193,202],"OAS1,":[195],"generally":[196],"considered":[197],"most":[199],"abundant":[200],"elutes":[204],"DU14":[206],"line":[208,283],"but":[209],"not":[210],"other":[212],"line.":[215,294],"In":[216],"this":[217,290],"phosphates":[220,278],"generated":[221],"enzymatic":[225],"reaction":[226],"is":[227,269],"conversion":[230],"substrate":[233],"2-amino-6-mercapto-7-methylpurine":[234,246],"ribonucleoside":[235],"(methylthioguanosine,":[236],"guanosine":[238],"analogue;":[239],"MESG)":[240],"purine":[243,252,266],"base":[244,267],"product,":[245],"ribose1-phosphate":[248],"via":[249],"catalyst":[251],"nucleoside":[253],"phosphorylase":[254],"(phosphorylase)":[255],"commercially":[258],"available":[259],"pyrophosphate":[260],"kit.":[261],"absorbance":[263],"product":[268],"measured":[270],"at":[271],"360":[272],"nm.":[273],"higher":[275],"levels":[276],"detected":[279,300],"DU145":[281],"indicates":[284],"more":[285],"CONCLUSION:":[295],"sensitivity":[305],"specificity,":[307],"which":[308],"could":[309],"help":[310],"avoiding":[316],"laborious":[318],"radioactive":[320],"methods.":[321]},"counts_by_year":[{"year":2019,"cited_by_count":1}],"updated_date":"2026-11-01T11:40:01.658920","created_date":"2016-06-24T00:00:00"}
