{"id":"https://openalex.org/W2295698","doi":null,"title":"Characterization of a high affinity cocaine binding site in rat brain","display_name":"Characterization of a high affinity cocaine binding site in rat brain","publication_year":1986,"publication_date":"1986-03-05","ids":{"openalex":"https://openalex.org/W2295698","mag":"2295698"},"language":"en","primary_location":{"id":"mag:2295698","doi":null,"is_oa":false,"landing_page_url":"http://www.osti.gov/scitech/biblio/7119424","pdf_url":null,"source":{"id":"https://openalex.org/S4306510388","display_name":"Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)","raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111742993","display_name":"David O. Calligaro","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"David O. Calligaro","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5110322797","display_name":"Mohyee E. Eldefrawi","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Mohyee E. Eldefrawi","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.1305,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":{"value":0.27754519,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10056","display_name":"Neurotransmitter Receptor Influence on Behavior","score":0.9409000277519226,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10056","display_name":"Neurotransmitter Receptor Influence on Behavior","score":0.9409000277519226,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10077","display_name":"Neuroscience and Neuropharmacology Research","score":0.040800001472234726,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.0052999998442828655,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cocaine-binding-site","display_name":"cocaine binding site","score":0.8889999985694885},{"id":"https://openalex.org/keywords/synaptic-membranes","display_name":"synaptic membranes","score":0.8650000095367432},{"id":"https://openalex.org/keywords/cocaine-analogs","display_name":"cocaine analogues","score":0.5630000233650208},{"id":"https://openalex.org/keywords/norcocaine","display_name":"norcocaine","score":0.8119999766349792},{"id":"https://openalex.org/keywords/tetracaine","display_name":"tetracaine","score":0.8899999856948853},{"id":"https://openalex.org/keywords/tricyclic-antidepressants","display_name":"tricyclic antidepressants","score":0.921999990940094},{"id":"https://openalex.org/keywords/phencyclidine","display_name":"phencyclidine","score":0.9950000047683716},{"id":"https://openalex.org/keywords/ph-sensitivity","display_name":"pH sensitivity","score":0.4690000116825104},{"id":"https://openalex.org/keywords/binding-isotherms","display_name":"binding isotherms","score":0.6129999756813049},{"id":"https://openalex.org/keywords/rat-brain","display_name":"rat brain","score":0.5879999995231628}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6967387199401855},{"id":"https://openalex.org/C107824862","wikidata":"https://www.wikidata.org/wiki/Q616005","display_name":"Binding site","level":2,"score":0.6524451375007629},{"id":"https://openalex.org/C2778851735","wikidata":"https://www.wikidata.org/wiki/Q407324","display_name":"Phencyclidine","level":4,"score":0.6178315281867981},{"id":"https://openalex.org/C2780283098","wikidata":"https://www.wikidata.org/wiki/Q4688960","display_name":"Affinities","level":2,"score":0.4379948675632477},{"id":"https://openalex.org/C2778455913","wikidata":"https://www.wikidata.org/wiki/Q419608","display_name":"Tetracaine","level":3,"score":0.4265308380126953},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.4054262340068817},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.20720455050468445},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.20265254378318787},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.10106334090232849},{"id":"https://openalex.org/C67018056","wikidata":"https://www.wikidata.org/wiki/Q414896","display_name":"NMDA receptor","level":3,"score":0.10015752911567688},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.0},{"id":"https://openalex.org/C2780149897","wikidata":"https://www.wikidata.org/wiki/Q216935","display_name":"Lidocaine","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:2295698","doi":null,"is_oa":false,"landing_page_url":"http://www.osti.gov/scitech/biblio/7119424","pdf_url":null,"source":{"id":"https://openalex.org/S4306510388","display_name":"Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.428600013256073,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1655645242","display_name":"GENERAL CLINICAL RESEARCH CENTER","funder_award_id":"M01RR000833","funder_id":"https://openalex.org/F4320337375","funder_display_name":"National Center for Research Resources"},{"id":"https://openalex.org/G4407923860","display_name":null,"funder_award_id":"DK-36639-01","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"},{"id":"https://openalex.org/G83723467","display_name":null,"funder_award_id":"DK 39275-02","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"}],"funders":[{"id":"https://openalex.org/F4320337357","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73"},{"id":"https://openalex.org/F4320337375","display_name":"National Center for Research Resources","ror":"https://ror.org/04pw6fb54"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":3,"referenced_works":["https://openalex.org/W120919092","https://openalex.org/W1714658201","https://openalex.org/W1760439020"],"related_works":["https://openalex.org/W1968940532","https://openalex.org/W1875134538","https://openalex.org/W1994024775","https://openalex.org/W2062665846","https://openalex.org/W2165629378","https://openalex.org/W2023653414","https://openalex.org/W2038430622","https://openalex.org/W1829908275","https://openalex.org/W2021188254","https://openalex.org/W2027908699"],"abstract_inverted_index":{"Binding":[0,99],"of":[1,18,56,64,76,100,110],"(/sup":[2,57,101,125,158],"3/H)cocaine":[3,58,102,126,159],"to":[4,61,79],"synaptic":[5],"membranes":[6,78],"from":[7],"whole":[8],"rat":[9],"brain":[10],"was":[11,59,72,90,105,138,152],"reversible":[12],"and":[13,38,66,71,120,169],"saturable.":[14],"Nonlinear":[15],"regression":[16],"analysis":[17],"binding":[19,23,55,86,127],"isotherms":[20],"indicated":[21],"two":[22],"affinities:":[24],"one":[25],"with":[26,41,131],"k/sub":[27],"d/":[28,43],"=":[29,34,44,49,144],"16":[30],"nM,":[31,46],"B/sub":[32,47],"max/":[33,48],"0.65":[35],"pmoles/mg":[36,51],"protein":[37],"the":[39,62,77,139,148,153],"other":[40],"K/sub":[42],"660":[45],"5.1":[50],"protein.":[52],"The":[53,134],"high-affinity":[54],"sensitive":[60],"actions":[63],"trypsin":[65],"chymotrypsin":[67],"but":[68],"not":[69],"carboxypeptidase,":[70],"eliminated":[73],"by":[74,107],"exposure":[75],"95/sup":[80],"0/C":[81],"for":[82],"5":[83],"min.":[84],"Specific":[85],"at":[87,92,96,128],"2":[88,129],"nM":[89,130],"higher":[91],"pH":[93,97],"8.8":[94],"than":[95],"7.0.":[98],"(15":[103],"nM)":[104],"inhibited":[106],"increasing":[108],"concentrations":[109],"Na/sup":[111],"+/":[112],"ions.":[113],"Several":[114,161],"cocaine":[115,135],"analogues,":[116],"neurotransmitter":[117],"uptake":[118,164],"inhibitors":[119],"local":[121,149],"anesthetics":[122],"displaced":[123],"specific":[124],"various":[132],"potencies.":[133],"analogue":[136],"(-)-norcocaine":[137],"most":[140],"potent":[141,155],"(IC/sub":[142],"50/":[143,173],"10":[145],"nM),":[146],"while":[147],"anesthetic":[150],"tetracaine":[151],"least":[154],"in":[156],"inhibiting":[157],"binding.":[160],"biogenic":[162],"amine":[163],"inhibitors,":[165],"including":[166],"tricyclic":[167],"antidepressants":[168],"phencyclidine,":[170],"had":[171],"IC/sub":[172],"values":[174],"below":[175],"..mu..M":[176],"concentrations.":[177]},"counts_by_year":[],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}