{"id":"https://openalex.org/W2292876430","doi":"https://doi.org/10.4049/jimmunol.182.supp.135.72","title":"NLRC4 is an innate immune detector of the bacterial type III secretion apparatus (135.72)","display_name":"NLRC4 is an innate immune detector of the bacterial type III secretion apparatus (135.72)","publication_year":2009,"publication_date":"2009-04-01","ids":{"openalex":"https://openalex.org/W2292876430","doi":"https://doi.org/10.4049/jimmunol.182.supp.135.72","mag":"2292876430"},"language":"en","primary_location":{"id":"doi:10.4049/jimmunol.182.supp.135.72","doi":"https://doi.org/10.4049/jimmunol.182.supp.135.72","is_oa":false,"landing_page_url":"https://doi.org/10.4049/jimmunol.182.supp.135.72","pdf_url":null,"source":{"id":"https://openalex.org/S38008053","display_name":"The Journal of Immunology","issn_l":"0022-1767","issn":["0022-1767","1550-6606"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4320800377","host_organization_name":"American Association of Immunologists","host_organization_lineage":["https://openalex.org/P4320800377"],"host_organization_lineage_names":["American Association of Immunologists"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Immunology","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5075858306","display_name":"Edward A. Miao","orcid":"https://orcid.org/0000-0001-7295-3490","observed_orcids":["https://orcid.org/0000-0001-7295-3490"]},"institutions":[{"id":"https://openalex.org/I3148997608","display_name":"Institute for Systems Biology","ror":"https://ror.org/02tpgw303","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I3148997608"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Edward A Miao","raw_affiliation_strings":["Institute for Systems Biology , Seattle, WA","1Institute for Systems Biology, Seattle, WA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Systems Biology , Seattle, WA","institution_ids":["https://openalex.org/I3148997608"]},{"raw_affiliation_string":"1Institute for Systems Biology, Seattle, WA","institution_ids":["https://openalex.org/I3148997608"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5025771001","display_name":"Dat P. Mao","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I3148997608","display_name":"Institute for Systems Biology","ror":"https://ror.org/02tpgw303","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I3148997608"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Dat Mao","raw_affiliation_strings":["Institute for Systems Biology , Seattle, WA","1Institute for Systems Biology, Seattle, WA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Systems Biology , Seattle, WA","institution_ids":["https://openalex.org/I3148997608"]},{"raw_affiliation_string":"1Institute for Systems Biology, Seattle, WA","institution_ids":["https://openalex.org/I3148997608"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040048917","display_name":"Natalya Yudkovsky","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I3148997608","display_name":"Institute for Systems Biology","ror":"https://ror.org/02tpgw303","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I3148997608"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Natalya Yudkovsky","raw_affiliation_strings":["Institute for Systems Biology , Seattle, WA","1Institute for Systems Biology, Seattle, WA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Systems Biology , Seattle, WA","institution_ids":["https://openalex.org/I3148997608"]},{"raw_affiliation_string":"1Institute for Systems Biology, Seattle, WA","institution_ids":["https://openalex.org/I3148997608"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5054277889","display_name":"Richard Bonnea","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I57206974","display_name":"New York University","ror":"https://ror.org/0190ak572","country_code":"US","type":"education","lineage":["https://openalex.org/I57206974"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Richard Bonnea","raw_affiliation_strings":["New York University , New York, NY","2New York University, New York, NY"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"New York University , New York, NY","institution_ids":["https://openalex.org/I57206974"]},{"raw_affiliation_string":"2New York University, New York, NY","institution_ids":["https://openalex.org/I57206974"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5063227404","display_name":"Alan A. Aderem","orcid":"https://orcid.org/0000-0003-4031-5499","observed_orcids":["https://orcid.org/0000-0003-4031-5499"]},"institutions":[{"id":"https://openalex.org/I3148997608","display_name":"Institute for Systems Biology","ror":"https://ror.org/02tpgw303","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I3148997608"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alan Aderem","raw_affiliation_strings":["Institute for Systems Biology , Seattle, WA","1Institute for Systems Biology, Seattle, WA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Systems Biology , Seattle, WA","institution_ids":["https://openalex.org/I3148997608"]},{"raw_affiliation_string":"1Institute for Systems Biology, Seattle, WA","institution_ids":["https://openalex.org/I3148997608"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3500,"currency":"USD","value_usd":3500},"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"182","issue":"Supplement_1","first_page":"135.72","last_page":"135.72"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10371","display_name":"Immune Response and Inflammation","score":0.8765000104904175,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10371","display_name":"Immune Response and Inflammation","score":0.8765000104904175,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11078","display_name":"Inflammasome and immune disorders","score":0.10779999941587448,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10943","display_name":"Escherichia coli research studies","score":0.002400000113993883,"subfield":{"id":"https://openalex.org/subfields/1310","display_name":"Endocrinology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/nlrc4","display_name":"NLRC4","score":0.9819999933242798},{"id":"https://openalex.org/keywords/interstitial-pneumonia-with-autoimmune-features","display_name":"interstitial pneumonia with autoimmune features","score":0.9290000200271606},{"id":"https://openalex.org/keywords/type-iii-secretion-system","display_name":"type III secretion system","score":0.8939999938011169},{"id":"https://openalex.org/keywords/innate-immune-recognition","display_name":"innate immune recognition","score":0.5989999771118164},{"id":"https://openalex.org/keywords/salmonella-typhimurium","display_name":"Salmonella typhimurium","score":0.8619999885559082},{"id":"https://openalex.org/keywords/salmonella-pathogenicity-island-1","display_name":"Salmonella pathogenicity island 1","score":0.765999972820282},{"id":"https://openalex.org/keywords/spi-2","display_name":"SPI-2","score":0.8989999890327454},{"id":"https://openalex.org/keywords/caspase-1","display_name":"caspase-1","score":0.6679999828338623},{"id":"https://openalex.org/keywords/interleukin-1beta","display_name":"IL-1\u03b2","score":0.6990000009536743},{"id":"https://openalex.org/keywords/il-18","display_name":"IL-18","score":0.953000009059906},{"id":"https://openalex.org/keywords/flagellin","display_name":"flagellin","score":0.6510000228881836},{"id":"https://openalex.org/keywords/macrophages","display_name":"macrophages","score":0.609000027179718},{"id":"https://openalex.org/keywords/nod-like-receptors","display_name":"NOD-like receptors","score":0.5149999856948853},{"id":"https://openalex.org/keywords/burkholderia-pseudomallei","display_name":"Burkholderia pseudomallei","score":0.746999979019165},{"id":"https://openalex.org/keywords/shigella-flexneri","display_name":"Shigella flexneri","score":0.9359999895095825},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.9869999885559082}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7909030914306641},{"id":"https://openalex.org/C136449434","wikidata":"https://www.wikidata.org/wiki/Q428253","display_name":"Innate immune system","level":3,"score":0.7619084119796753},{"id":"https://openalex.org/C51785407","wikidata":"https://www.wikidata.org/wiki/Q2627568","display_name":"Effector","level":2,"score":0.7365331053733826},{"id":"https://openalex.org/C60987743","wikidata":"https://www.wikidata.org/wiki/Q1460232","display_name":"Virulence","level":3,"score":0.7218838930130005},{"id":"https://openalex.org/C38117629","wikidata":"https://www.wikidata.org/wiki/Q24788421","display_name":"Flagellin","level":3,"score":0.7176594734191895},{"id":"https://openalex.org/C130211564","wikidata":"https://www.wikidata.org/wiki/Q21199165","display_name":"Type three secretion system","level":4,"score":0.6988808512687683},{"id":"https://openalex.org/C49039625","wikidata":"https://www.wikidata.org/wiki/Q84230","display_name":"Secretion","level":2,"score":0.6420947909355164},{"id":"https://openalex.org/C56432331","wikidata":"https://www.wikidata.org/wiki/Q18044860","display_name":"NLRC4","level":5,"score":0.5787283778190613},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.575532078742981},{"id":"https://openalex.org/C2781217973","wikidata":"https://www.wikidata.org/wiki/Q1644417","display_name":"Shigella flexneri","level":4,"score":0.5366799831390381},{"id":"https://openalex.org/C62130838","wikidata":"https://www.wikidata.org/wiki/Q5196163","display_name":"Pathogenicity island","level":4,"score":0.4521194100379944},{"id":"https://openalex.org/C2777209026","wikidata":"https://www.wikidata.org/wiki/Q412405","display_name":"Inflammasome","level":3,"score":0.39910680055618286},{"id":"https://openalex.org/C8891405","wikidata":"https://www.wikidata.org/wiki/Q1059","display_name":"Immune system","level":2,"score":0.37796860933303833},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.347430944442749},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3328832685947418},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.24509435892105103},{"id":"https://openalex.org/C139964883","wikidata":"https://www.wikidata.org/wiki/Q14901964","display_name":"Caspase 1","level":4,"score":0.18240958452224731},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.17316842079162598},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.15776270627975464},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.4049/jimmunol.182.supp.135.72","doi":"https://doi.org/10.4049/jimmunol.182.supp.135.72","is_oa":false,"landing_page_url":"https://doi.org/10.4049/jimmunol.182.supp.135.72","pdf_url":null,"source":{"id":"https://openalex.org/S38008053","display_name":"The Journal of Immunology","issn_l":"0022-1767","issn":["0022-1767","1550-6606"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4320800377","host_organization_name":"American Association of Immunologists","host_organization_lineage":["https://openalex.org/P4320800377"],"host_organization_lineage_names":["American Association of Immunologists"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Immunology","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.46810001134872437,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2155538302","https://openalex.org/W2028371610","https://openalex.org/W2154796864","https://openalex.org/W6962658096","https://openalex.org/W2747632774","https://openalex.org/W4225879965","https://openalex.org/W2156120317","https://openalex.org/W4299996350","https://openalex.org/W4404470018","https://openalex.org/W7118601424"],"abstract_inverted_index":{"Abstract":[0],"The":[1],"mammalian":[2],"innate":[3],"immune":[4],"system":[5],"uses":[6],"Toll-like":[7],"receptors":[8],"(TLRs)":[9],"and":[10,40,105,110,156,173,256],"Nod-LRRs":[11],"(NLRs)":[12],"to":[13,62,66,77,188],"detect":[14],"microbial":[15],"components":[16],"during":[17],"infection.":[18],"Often":[19],"these":[20],"molecules":[21],"work":[22],"in":[23,133,180],"concert;":[24],"for":[25,124,178,240],"example,":[26],"the":[27,31,34,37,67,79,87,93,152,160,189,196,205,215,244,248,252],"TLRs":[28],"can":[29],"stimulate":[30],"production":[32],"of":[33,36,69,92,191,217,247],"pro-forms":[35],"cytokines":[38],"IL-1\u03b2":[39],"IL-18,":[41],"whereas":[42],"certain":[43],"NLRs":[44],"trigger":[45],"their":[46],"subsequent":[47],"proteolytic":[48],"processing":[49],"via":[50],"caspase":[51],"1.":[52],"Gram":[53],"negative":[54],"bacteria":[55],"utilize":[56],"type":[57],"III":[58],"secretion":[59],"systems":[60],"(T3SS)":[61],"deliver":[63],"virulence":[64,179,199,249],"factors":[65],"cytosol":[68],"host":[70,218],"cells,":[71],"where":[72,232],"they":[73],"modulate":[74],"cell":[75],"physiology":[76],"favour":[78],"pathogen.":[80],"We":[81],"show":[82],"here":[83],"that":[84,121,185,208,224,228],"NLRC4/Ipaf":[85],"detects":[86],"basal":[88],"body":[89],"rod":[90,115,153,161],"component":[91],"T3SS":[94],"apparatus":[95,207],"(rod":[96],"protein)":[97],"from":[98,227],"S.":[99,141],"typhimurium":[100,142],"(PrgJ),":[101],"Escherichia":[102],"coli":[103],"(EprJ":[104],"EscI),":[106],"Burkholderia":[107],"pseudomallei":[108],"(BsaK),":[109],"Shigella":[111],"flexneri":[112],"(MxiI).":[113],"These":[114],"proteins":[116],"share":[117],"a":[118,128,221,238],"sequence":[119],"motif":[120,130],"is":[122,131,136,166,171,176,209,225],"essential":[123],"detection":[125,246],"by":[126,139,168,194,203,230],"NLRC4;":[127],"similar":[129],"found":[132],"flagellin":[134],"which":[135,150,158],"also":[137],"detected":[138,167,236],"NLRC4.":[140],"has":[143],"two":[144],"T3SS:":[145],"Salmonella":[146],"pathogenicity":[147],"island-1":[148],"(SPI-1),":[149],"encodes":[151,159],"protein":[154,162],"PrgJ,":[155],"SPI-2,":[157],"SsaI.":[163],"While":[164],"PrgJ":[165],"NLRC4,":[169],"SsaI":[170],"not,":[172],"this":[174],"evasion":[175],"required":[177],"mice.":[181],"Our":[182],"results":[183],"indicate":[184],"macrophages":[186],"respond":[187],"activity":[190],"T3SS,":[192],"not":[193],"sensing":[195],"specific":[197,245],"translocated":[198,233],"factors,":[200],"but":[201],"rather":[202],"detecting":[204],"translocation":[206],"conserved":[210],"between":[211,254],"pathogens.":[212],"This":[213],"reduces":[214],"number":[216],"detectors":[219],"required,":[220],"survival":[222],"strategy":[223],"divergent":[226],"used":[229],"plants,":[231],"effectors":[234],"are":[235],"on":[237],"gene":[239,241],"basis.":[242],"Furthermore,":[243],"machinery":[250],"permits":[251],"discrimination":[253],"pathogenic":[255,258],"non":[257],"bacteria.":[259]},"counts_by_year":[],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2016-06-24T00:00:00"}