{"id":"https://openalex.org/W2292818646","doi":"https://doi.org/10.1371/journal.pone.0149751","title":"The Non-Specific Binding of Fluorescent-Labeled MiRNAs on Cell Surface by Hydrophobic Interaction","display_name":"The Non-Specific Binding of Fluorescent-Labeled MiRNAs on Cell Surface by Hydrophobic Interaction","publication_year":2016,"publication_date":"2016-03-01","ids":{"openalex":"https://openalex.org/W2292818646","doi":"https://doi.org/10.1371/journal.pone.0149751","mag":"2292818646","pmid":"https://pubmed.ncbi.nlm.nih.gov/26930565","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/4773022"},"language":"en","primary_location":{"id":"doi:10.1371/journal.pone.0149751","doi":"https://doi.org/10.1371/journal.pone.0149751","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0149751","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0149751&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLOS ONE","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0149751&type=printable","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Ting Lu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I154099455","display_name":"Shandong University","ror":"https://ror.org/0207yh398","country_code":"CN","type":"education","lineage":["https://openalex.org/I154099455"]},{"id":"https://openalex.org/I4210116517","display_name":"Shandong Provincial Hospital","ror":"https://ror.org/02ar2nf05","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210116517"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Ting Lu","raw_affiliation_strings":["Division of Endocrinology and Metabolism, Shandong Provincial Hospital affiliated to Shandong University, Jinan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Endocrinology and Metabolism, Shandong Provincial Hospital affiliated to Shandong University, Jinan, China","institution_ids":["https://openalex.org/I154099455","https://openalex.org/I4210116517"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5029072358","display_name":"Zongwei Lin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1327237609","display_name":"Ministry of Education of the People's Republic of China","ror":"https://ror.org/01mv9t934","country_code":"CN","type":"government","lineage":["https://openalex.org/I1327237609","https://openalex.org/I4210127390"]},{"id":"https://openalex.org/I4210161528","display_name":"Qilu Hospital of Shandong University","ror":"https://ror.org/056ef9489","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210161528"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Zongwei Lin","raw_affiliation_strings":["The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health; The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Hospital of Shandong University, Jinan, China","The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health","The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Hospital of Shandong University, Jinan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health; The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Hospital of Shandong University, Jinan, China","institution_ids":["https://openalex.org/I4210161528"]},{"raw_affiliation_string":"The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health","institution_ids":["https://openalex.org/I1327237609"]},{"raw_affiliation_string":"The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Hospital of Shandong University, Jinan, China","institution_ids":["https://openalex.org/I4210161528"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102705618","display_name":"Jianwei Ren","orcid":"https://orcid.org/0000-0003-0142-2603","observed_orcids":["https://orcid.org/0000-0003-0142-2603"]},"institutions":[{"id":"https://openalex.org/I4210160531","display_name":"Chinese People's Liberation Army","ror":"https://ror.org/05tf9r976","country_code":"CN","type":"government","lineage":["https://openalex.org/I4210160531"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jianwei Ren","raw_affiliation_strings":["Health Division of Guard Bureau, General Staff Department of Chinese PLA, Beijing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Health Division of Guard Bureau, General Staff Department of Chinese PLA, Beijing, China","institution_ids":["https://openalex.org/I4210160531"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Peng Yao","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Peng Yao","raw_affiliation_strings":["Traditional Chinese Medicine Department, Jinan Firefighting Hospital, Jinan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Traditional Chinese Medicine Department, Jinan Firefighting Hospital, Jinan, China","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100460517","display_name":"Xiaowei Wang","orcid":"https://orcid.org/0000-0003-0940-1882","observed_orcids":["https://orcid.org/0000-0003-0940-1882"]},"institutions":[{"id":"https://openalex.org/I1327237609","display_name":"Ministry of Education of the People's Republic of China","ror":"https://ror.org/01mv9t934","country_code":"CN","type":"government","lineage":["https://openalex.org/I1327237609","https://openalex.org/I4210127390"]},{"id":"https://openalex.org/I4210161528","display_name":"Qilu Hospital of Shandong University","ror":"https://ror.org/056ef9489","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210161528"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xiaowei Wang","raw_affiliation_strings":["The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health; The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Hospital of Shandong University, Jinan, China","The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health","The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Hospital of Shandong University, Jinan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health; The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Hospital of Shandong University, Jinan, China","institution_ids":["https://openalex.org/I4210161528"]},{"raw_affiliation_string":"The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health","institution_ids":["https://openalex.org/I1327237609"]},{"raw_affiliation_string":"The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Hospital of Shandong University, Jinan, China","institution_ids":["https://openalex.org/I4210161528"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100407680","display_name":"Zhe Wang","orcid":"https://orcid.org/0000-0003-4036-7969","observed_orcids":["https://orcid.org/0000-0003-4036-7969"]},"institutions":[{"id":"https://openalex.org/I154099455","display_name":"Shandong University","ror":"https://ror.org/0207yh398","country_code":"CN","type":"education","lineage":["https://openalex.org/I154099455"]},{"id":"https://openalex.org/I4210116517","display_name":"Shandong Provincial Hospital","ror":"https://ror.org/02ar2nf05","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210116517"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Zhe Wang","raw_affiliation_strings":["Division of Endocrinology and Metabolism, Shandong Provincial Hospital affiliated to Shandong University, Jinan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Endocrinology and Metabolism, Shandong Provincial Hospital affiliated to Shandong University, Jinan, China","institution_ids":["https://openalex.org/I154099455","https://openalex.org/I4210116517"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5014902238","display_name":"Qunye Zhang","orcid":"https://orcid.org/0000-0003-1535-9182","observed_orcids":["https://orcid.org/0000-0003-1535-9182"]},"institutions":[{"id":"https://openalex.org/I1327237609","display_name":"Ministry of Education of the People's Republic of China","ror":"https://ror.org/01mv9t934","country_code":"CN","type":"government","lineage":["https://openalex.org/I1327237609","https://openalex.org/I4210127390"]},{"id":"https://openalex.org/I4210161528","display_name":"Qilu Hospital of Shandong University","ror":"https://ror.org/056ef9489","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210161528"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Qunye Zhang","raw_affiliation_strings":["The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health; The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Hospital of Shandong University, Jinan, China","The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health","The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Hospital of Shandong University, Jinan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health; The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Hospital of Shandong University, Jinan, China","institution_ids":["https://openalex.org/I4210161528"]},{"raw_affiliation_string":"The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health","institution_ids":["https://openalex.org/I1327237609"]},{"raw_affiliation_string":"The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Hospital of Shandong University, Jinan, China","institution_ids":["https://openalex.org/I4210161528"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5014902238","https://openalex.org/A5100407680"],"corresponding_institution_ids":["https://openalex.org/I1327237609","https://openalex.org/I154099455","https://openalex.org/I4210116517","https://openalex.org/I4210161528"],"apc_list":{"value":2382,"currency":"USD","value_usd":2382},"apc_paid":{"value":2382,"currency":"USD","value_usd":2382},"fwci":0.5196,"has_fulltext":true,"cited_by_count":11,"citation_normalized_percentile":{"value":0.68349807,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":"11","issue":"3","first_page":"e0149751","last_page":"e0149751"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.986299991607666,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.986299991607666,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10062","display_name":"MicroRNA in disease regulation","score":0.01119999960064888,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/microrna","display_name":"microRNA","score":0.8690000176429749},{"id":"https://openalex.org/keywords/fluorescent-labeling","display_name":"fluorescent labeling","score":0.7730000019073486},{"id":"https://openalex.org/keywords/lipofection","display_name":"lipofection","score":0.8790000081062317},{"id":"https://openalex.org/keywords/nonspecific-binding","display_name":"non-specific binding","score":0.8059999942779541},{"id":"https://openalex.org/keywords/hydrophobic-interactions","display_name":"hydrophobic interactions","score":0.9340000152587891},{"id":"https://openalex.org/keywords/cell-surface","display_name":"cell surface","score":0.777999997138977},{"id":"https://openalex.org/keywords/transfection-efficiency","display_name":"transfection efficiency","score":0.9039999842643738},{"id":"https://openalex.org/keywords/fluorescent-dyes","display_name":"fluorescent dyes","score":0.753000020980835},{"id":"https://openalex.org/keywords/cy5","display_name":"Cy5","score":0.7319999933242798},{"id":"https://openalex.org/keywords/fam","display_name":"FAM","score":0.8849999904632568},{"id":"https://openalex.org/keywords/adherent-cells","display_name":"adherent cells","score":0.6100000143051147},{"id":"https://openalex.org/keywords/suspension-cultured-cells","display_name":"suspension-cultured cells","score":0.8840000033378601},{"id":"https://openalex.org/keywords/electrostatic-interactions","display_name":"electrostatic interactions","score":0.5049999952316284},{"id":"https://openalex.org/keywords/organic-solvents","display_name":"organic solvents","score":0.6819999814033508}],"concepts":[{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.7478866577148438},{"id":"https://openalex.org/C2777366897","wikidata":"https://www.wikidata.org/wiki/Q847482","display_name":"HeLa","level":3,"score":0.6619768142700195},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.5913670063018799},{"id":"https://openalex.org/C49923114","wikidata":"https://www.wikidata.org/wiki/Q17139621","display_name":"Lipofectamine","level":5,"score":0.5673559308052063},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5018031597137451},{"id":"https://openalex.org/C24107716","wikidata":"https://www.wikidata.org/wiki/Q123619","display_name":"Nucleic acid","level":2,"score":0.48799625039100647},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.4758322238922119},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.46766507625579834},{"id":"https://openalex.org/C2777324032","wikidata":"https://www.wikidata.org/wiki/Q412922","display_name":"Cyanine","level":3,"score":0.4671221375465393},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.4486081600189209},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.43905413150787354},{"id":"https://openalex.org/C2778015030","wikidata":"https://www.wikidata.org/wiki/Q410922","display_name":"Fluorescein","level":3,"score":0.4380652904510498},{"id":"https://openalex.org/C145059251","wikidata":"https://www.wikidata.org/wiki/Q310899","display_name":"microRNA","level":3,"score":0.4360671043395996},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.42225217819213867},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.3831423819065094},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3314036726951599},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.11142414808273315},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.08023202419281006},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.07169482111930847},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C92087593","wikidata":"https://www.wikidata.org/wiki/Q427389","display_name":"Vector (molecular biology)","level":4,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D002232","descriptor_name":"Carbocyanines","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002232","descriptor_name":"Carbocyanines","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D002462","descriptor_name":"Cell Membrane","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005434","descriptor_name":"Flow Cytometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005456","descriptor_name":"Fluorescent Dyes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005456","descriptor_name":"Fluorescent Dyes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D006367","descriptor_name":"HeLa Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008081","descriptor_name":"Liposomes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D018613","descriptor_name":"Microscopy, Confocal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020014","descriptor_name":"K562 Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D035683","descriptor_name":"MicroRNAs","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D035683","descriptor_name":"MicroRNAs","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D035683","descriptor_name":"MicroRNAs","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D036681","descriptor_name":"Fluorescence Recovery After Photobleaching","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D055672","descriptor_name":"Static Electricity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D057927","descriptor_name":"Hydrophobic and Hydrophilic Interactions","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":5,"locations":[{"id":"doi:10.1371/journal.pone.0149751","doi":"https://doi.org/10.1371/journal.pone.0149751","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0149751","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0149751&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLOS ONE","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:4773022","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4773022","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS One","raw_type":"Text"},{"id":"pmid:26930565","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/26930565","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PloS one","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:0b615083a77c4e2984bb04d9f7850d03","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/0b615083a77c4e2984bb04d9f7850d03","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"PLoS ONE, Vol 11, Iss 3, p e0149751 (2016)","raw_type":"article"},{"id":"pmh:oai:figshare.com:article/3087880","doi":null,"is_oa":true,"landing_page_url":"https://figshare.com/articles/dataset/The_Non_Specific_Binding_of_Fluorescent_Labeled_MiRNAs_on_Cell_Surface_by_Hydrophobic_Interaction/3087880","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Dataset"}],"best_oa_location":{"id":"doi:10.1371/journal.pone.0149751","doi":"https://doi.org/10.1371/journal.pone.0149751","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0149751","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0149751&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLOS ONE","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.4043000042438507,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G2070218498","display_name":null,"funder_award_id":"ZR2010HL012","funder_id":"https://openalex.org/F4320324174","funder_display_name":"Natural Science Foundation of Shandong Province"},{"id":"https://openalex.org/G4441838823","display_name":null,"funder_award_id":"81570712","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G4465470754","display_name":null,"funder_award_id":"81370270","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G4821505058","display_name":null,"funder_award_id":"91439111","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G5416941839","display_name":null,"funder_award_id":"JQ201519","funder_id":"https://openalex.org/F4320324174","funder_display_name":"Natural Science Foundation of Shandong Province"},{"id":"https://openalex.org/G7142450366","display_name":"\u4ee5miR-363\u4e3a\u91cd\u8981\u8282\u70b9\u7684miRNA\u7f51\u7edc\u5f02\u5e38\u5728Graves\u75c5Treg\u7ec6\u80de\u529f\u80fd\u5931\u8c03\u4e2d\u7684\u4f5c\u7528\u53ca\u673a\u5236","funder_award_id":"81370890","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"}],"funders":[{"id":"https://openalex.org/F4320321001","display_name":"National Natural Science Foundation of China","ror":"https://ror.org/01h0zpd94"},{"id":"https://openalex.org/F4320324174","display_name":"Natural Science Foundation of Shandong Province","ror":null}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2292818646.pdf","grobid_xml":"https://content.openalex.org/works/W2292818646.grobid-xml"},"referenced_works_count":32,"referenced_works":["https://openalex.org/W1801213493","https://openalex.org/W1969338185","https://openalex.org/W1975332416","https://openalex.org/W1980226233","https://openalex.org/W1985325702","https://openalex.org/W1986137107","https://openalex.org/W1996447938","https://openalex.org/W2002061154","https://openalex.org/W2004739654","https://openalex.org/W2012068153","https://openalex.org/W2020710960","https://openalex.org/W2021414543","https://openalex.org/W2023620453","https://openalex.org/W2029287872","https://openalex.org/W2037483637","https://openalex.org/W2047082928","https://openalex.org/W2052445951","https://openalex.org/W2062595050","https://openalex.org/W2070380977","https://openalex.org/W2077373908","https://openalex.org/W2091220084","https://openalex.org/W2091510202","https://openalex.org/W2097233439","https://openalex.org/W2104544316","https://openalex.org/W2115093090","https://openalex.org/W2119675020","https://openalex.org/W2130400901","https://openalex.org/W2133968430","https://openalex.org/W2135836598","https://openalex.org/W2148273027","https://openalex.org/W2157029838","https://openalex.org/W2168858738"],"related_works":["https://openalex.org/W2345132869","https://openalex.org/W2392804297","https://openalex.org/W1032894858","https://openalex.org/W4212788518","https://openalex.org/W7096106970","https://openalex.org/W2134214562","https://openalex.org/W2966549453","https://openalex.org/W2090349722","https://openalex.org/W1990874508","https://openalex.org/W7096838991"],"abstract_inverted_index":{"BACKGROUND:":[0],"MicroRNAs":[1],"are":[2,26],"small":[3],"noncoding":[4],"RNAs":[5],"about":[6,44,53],"22":[7],"nt":[8],"long":[9],"that":[10,103,225],"play":[11],"key":[12],"roles":[13],"in":[14,148,245],"almost":[15],"all":[16],"biological":[17],"processes":[18],"and":[19,24,34,57,78,88,96,121,266],"diseases.":[20],"The":[21,100,129],"fluorescent":[22,72,109,269],"labeling":[23],"lipofection":[25,60,82,127],"two":[27],"common":[28],"methods":[29,260],"for":[30,279],"changing":[31],"the":[32,36,45,54,85,115,158,164,175,187,193,199,205,210,216,226,233,246,254,263,276,280],"levels":[33],"locating":[35],"position":[37],"of":[38,47,59,61,117,131,157,166,174,181,189,248,282],"cellular":[39,255],"miRNAs.":[40,63],"Despite":[41],"many":[42],"studies":[43],"mechanism":[46,90],"DNA/RNA":[48],"lipofection,":[49],"little":[50],"is":[51],"known":[52],"characteristics,":[55],"mechanisms":[56],"specificity":[58],"fluorescent-labeled":[62,227],"METHODS":[64],"AND":[65],"RESULTS:":[66],"Therefore,":[67,258],"miRNAs":[68,104,132,161,228],"labeled":[69,105,133,160],"with":[70,106,134],"different":[71],"dyes":[73,270],"were":[74,91,169,183],"transfected":[75,170],"into":[76,171],"adherent":[77,118],"suspension":[79],"cells":[80,119,123,140,168],"using":[81],"reagent.":[83,128],"Then,":[84],"non-specific":[86,207],"binding":[87,130,162,185],"its":[89],"investigated":[92],"by":[93,236],"flow":[94],"cytometer":[95],"laser":[97],"confocal":[98],"microscopy.":[99],"results":[101,223],"showed":[102],"Cy5":[107],"(cyanine":[108],"dye)":[110],"could":[111,197,214,229],"firmly":[112],"bind":[113,231],"to":[114,138,163,186,232,242,253,261,275],"surface":[116,165,188,235],"(Hela)":[120],"suspended":[122],"(K562)":[124],"even":[125],"without":[126],"FAM":[135],"(carboxyl":[136],"fluorescein)":[137],"K562":[139,190],"was":[141,145,154],"obvious,":[142],"but":[143,209],"it":[144],"not":[146,203],"significant":[147,243],"Hela":[149,167],"cells.":[150,191],"After":[151],"lipofectamine":[152],"reagent":[153],"added,":[155],"most":[156,180],"fluorescently":[159],"intra-cell":[172],"because":[173],"high":[176],"transfection":[177,249,264],"efficiency,":[178],"however,":[179],"them":[182],"still":[184],"Moreover,":[192],"high-salt":[194],"buffer":[195],"which":[196,213],"destroy":[198,215],"electrostatic":[200],"interactions":[201,218],"did":[202],"affect":[204],"above-mentioned":[206],"binding,":[208],"organic":[211],"solvent":[212],"hydrophobic":[217,237],"eliminated":[219],"it.":[220],"CONCLUSIONS:":[221],"These":[222],"implied":[224],"non-specifically":[230],"cell":[234,277],"interaction.":[238],"It":[239],"would":[240],"lead":[241],"errors":[244],"estimation":[247],"efficiency":[250,265],"only":[251],"according":[252,274],"fluorescence":[256],"intensity.":[257],"other":[259],"evaluate":[262],"more":[267],"appropriate":[268],"should":[271],"be":[272],"used":[273],"types":[278],"accuracy":[281],"results.":[283]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":1},{"year":2021,"cited_by_count":4},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2016,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}