{"id":"https://openalex.org/W2292064586","doi":null,"title":"Characterization of the alginate lyases from Vibrio","display_name":"Characterization of the alginate lyases from Vibrio","publication_year":2015,"publication_date":"2015-01-01","ids":{"openalex":"https://openalex.org/W2292064586","mag":"2292064586"},"language":"en","primary_location":{"id":"mag:2292064586","doi":null,"is_oa":false,"landing_page_url":"https://www.scs.illinois.edu/~zhaogrp/publications/HZ195.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5014837616","display_name":"Ahmet H. Badur","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Ahmet H. Badur","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089743153","display_name":"Sujit Sadashiv Jagtap","orcid":"https://orcid.org/0000-0001-9402-2259","observed_orcids":["https://orcid.org/0000-0001-9402-2259"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Sujit Sadashiv Jagtap","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0001-9402-2259","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113211203","display_name":"Geethika Yalamanchili","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Geethika Yalamanchili","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5022372411","display_name":"Jung-Kul Lee","orcid":"https://orcid.org/0000-0001-7384-5301","observed_orcids":["https://orcid.org/0000-0001-7384-5301"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jung-Kul Lee","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0001-7384-5301","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002071430","display_name":"Huimin","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Huimin","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5005829958","display_name":"Christopher V. Rao","orcid":"https://orcid.org/0000-0001-5606-4204","observed_orcids":["https://orcid.org/0000-0001-5606-4204","https://orcid.org/0000-0001-5470-3981"]},"institutions":[{"id":"https://openalex.org/I157725225","display_name":"University of Illinois Urbana-Champaign","ror":"https://ror.org/047426m28","country_code":"US","type":"education","lineage":["https://openalex.org/I157725225"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Christopher V. Rao","raw_affiliation_strings":["***University of Illinois at Urbana-Champaign"],"raw_orcid":"https://orcid.org/0000-0001-5606-4204","affiliations":[{"raw_affiliation_string":"***University of Illinois at Urbana-Champaign","institution_ids":["https://openalex.org/I157725225"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.8628000020980835,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.8628000020980835,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12192","display_name":"Polysaccharides Composition and Applications","score":0.0697999969124794,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10869","display_name":"Polysaccharides and Plant Cell Walls","score":0.009999999776482582,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/alginate-lyase","display_name":"alginate lyase","score":0.984000027179718},{"id":"https://openalex.org/keywords/vibrio-splendidus","display_name":"Vibrio splendidus","score":0.9570000171661377},{"id":"https://openalex.org/keywords/alginate-degradation","display_name":"alginate degradation","score":0.9610000252723694},{"id":"https://openalex.org/keywords/marine-bacterioplankton","display_name":"marine bacterioplankton","score":0.7979999780654907},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.6460000276565552},{"id":"https://openalex.org/keywords/substrate-specificity","display_name":"substrate specificity","score":0.7639999985694885},{"id":"https://openalex.org/keywords/beta-1-4-glycosidic-bond-cleavage","display_name":"\u03b2-1,4-glycosidic bond cleavage","score":0.8299999833106995},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.7710000276565552}],"concepts":[{"id":"https://openalex.org/C19014433","wikidata":"https://www.wikidata.org/wiki/Q5130995","display_name":"Cleave","level":3,"score":0.8271005153656006},{"id":"https://openalex.org/C150487720","wikidata":"https://www.wikidata.org/wiki/Q422553","display_name":"Glycosidic bond","level":3,"score":0.716781497001648},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5655361413955688},{"id":"https://openalex.org/C2776154503","wikidata":"https://www.wikidata.org/wiki/Q753490","display_name":"Vibrio","level":3,"score":0.5355373024940491},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5300785303115845},{"id":"https://openalex.org/C2776422119","wikidata":"https://www.wikidata.org/wiki/Q910922","display_name":"Vibrionaceae","level":3,"score":0.486827552318573},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4732906222343445},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.4633754789829254},{"id":"https://openalex.org/C56856141","wikidata":"https://www.wikidata.org/wiki/Q883112","display_name":"Enzyme kinetics","level":4,"score":0.44784632325172424},{"id":"https://openalex.org/C153568844","wikidata":"https://www.wikidata.org/wiki/Q96392018","display_name":"Marine bacteriophage","level":3,"score":0.44360560178756714},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.4027113914489746},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.28906530141830444},{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.11256951093673706},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:2292064586","doi":null,"is_oa":false,"landing_page_url":"https://www.scs.illinois.edu/~zhaogrp/publications/HZ195.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":49,"referenced_works":["https://openalex.org/W236283112","https://openalex.org/W1498490482","https://openalex.org/W1499500424","https://openalex.org/W1518694802","https://openalex.org/W1543282543","https://openalex.org/W1870332663","https://openalex.org/W1963751067","https://openalex.org/W1975932545","https://openalex.org/W1976789868","https://openalex.org/W1983125435","https://openalex.org/W1985164519","https://openalex.org/W1988012549","https://openalex.org/W1992263762","https://openalex.org/W1999558053","https://openalex.org/W2001229509","https://openalex.org/W2010911897","https://openalex.org/W2013387016","https://openalex.org/W2016796480","https://openalex.org/W2018440937","https://openalex.org/W2024314886","https://openalex.org/W2027491421","https://openalex.org/W2029667189","https://openalex.org/W2035625052","https://openalex.org/W2038239133","https://openalex.org/W2048701241","https://openalex.org/W2051922476","https://openalex.org/W2055595832","https://openalex.org/W2055636276","https://openalex.org/W2065687317","https://openalex.org/W2066013240","https://openalex.org/W2078847935","https://openalex.org/W2088607306","https://openalex.org/W2090655671","https://openalex.org/W2094201044","https://openalex.org/W2095183256","https://openalex.org/W2100897835","https://openalex.org/W2104229815","https://openalex.org/W2122337332","https://openalex.org/W2125152270","https://openalex.org/W2132366841","https://openalex.org/W2135005420","https://openalex.org/W2136250029","https://openalex.org/W2137885656","https://openalex.org/W2144444084","https://openalex.org/W2146237557","https://openalex.org/W2149525061","https://openalex.org/W2168621094","https://openalex.org/W2169522128","https://openalex.org/W2320203601"],"related_works":["https://openalex.org/W2016629278","https://openalex.org/W2269907786","https://openalex.org/W2608518163","https://openalex.org/W2135005420","https://openalex.org/W2326369533","https://openalex.org/W2055636276","https://openalex.org/W4367047770","https://openalex.org/W4310195199","https://openalex.org/W2314733326","https://openalex.org/W1543282543"],"abstract_inverted_index":{"18":[0],"Alginate":[1],"lyases":[2,22,54,69,203],"are":[3],"enzymes":[4],"that":[5,32],"degrade":[6,205],"alginate":[7,21,36,107,146,202,206],"through":[8],"\u03b2-elimination":[9],"of":[10,108,139,210],"the":[11,20,175,194],"glycosidic":[12],"bond":[13],"19":[14],"into":[15,207],"smaller":[16],"oligomers.":[17,211],"We":[18,42],"investigated":[19],"from":[23,63],"Vibrio":[24],"splendidus":[25,65],"12B01,":[26],"a":[27,88,101],"20":[28],"marine":[29,89],"bacterioplankton":[30],"species":[31],"can":[33],"grow":[34],"on":[35],"as":[37],"its":[38],"sole":[39],"carbon":[40],"source.":[41],"21":[43],"identified,":[44],"purified,":[45],"and":[46,60,80,95,127,143,161,168,181,183,186],"characterized":[47],"four":[48,68,201],"polysaccharide":[49],"lyase":[50],"family":[51],"7":[52,150],"alginates":[53],"\u2013":[55,62],"22":[56,152],"AlyA,":[57,92,140],"AlyB,":[58,93,141],"AlyD,":[59,94,142],"AlyE":[61,96,144,187],"V.":[64],"12B01.":[66],"The":[67,135,200],"were":[70,97,147,170,188],"found":[71,98,171,189],"to":[72,99,173,190],"have":[73],"23":[74],"optimal":[75],"activity":[76],"between":[77,179],"pH":[78],"7.5-8.5":[79],"at":[81],"20-25\u00b0C,":[82],"consistent":[83],"with":[84],"their":[85],"use":[86],"in":[87],"24":[90],"environment.":[91],"exhibit":[100],"turnover":[102],"number":[103],"(kcat)":[104],"for":[105],"25":[106],"0.60":[109],"\u00b1":[110,116,122,129,149,153,157,163],"0.02":[111],"s":[112,118,124,131],"-1":[113,119,125,132],",":[114,120,126,133],"3.7":[115],"0.3":[117],"4.5":[121],"0.5":[123],"7.1":[128],"0.2":[130],"respectively.":[134,166],"Km":[136],"26":[137],"values":[138],"toward":[145],"36":[148],"\u03bcM,":[151,155,159,165],"5":[154],"60":[156],"2":[158],"27":[160],"123":[162],"6":[164],"AlyA":[167],"AlyB":[169],"principally":[172,192],"cleave":[174,193],"\u03b2-1,4":[176],"bonds":[177,196],"28":[178],"\u03b2-D-mannuronate":[180],"\u03b1-L-guluronate":[182,198],"subunits;":[184],"AlyD":[185],"29":[191],"\u03b1-1,4":[195],"involving":[197],"subunits.":[199],"30":[204],"longer":[208],"chains":[209],"31":[212]},"counts_by_year":[],"updated_date":"2026-10-16T09:59:22.235173","created_date":"2016-06-24T00:00:00"}