{"id":"https://openalex.org/W2291225357","doi":null,"title":"Identification and characterisation of novel Legionella pneumophila virulence genes","display_name":"Identification and characterisation of novel Legionella pneumophila virulence genes","publication_year":2015,"publication_date":"2015-01-01","ids":{"openalex":"https://openalex.org/W2291225357","mag":"2291225357"},"language":"en","primary_location":{"id":"pmh:oai:jupiter.its.unimelb.edu.au:11343/56471","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/11343/56471","pdf_url":"http://hdl.handle.net/11343/56471","source":{"id":"https://openalex.org/S4377196259","display_name":"Minerva Access (University of Melbourne)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I165779595","host_organization_name":"The University of Melbourne","host_organization_lineage":["https://openalex.org/I165779595"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"PhD thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://hdl.handle.net/11343/56471","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5112463513","display_name":"Adam J. Vogrin","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Adam J. Vogrin","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5112463513"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11916","display_name":"Legionella and Acanthamoeba research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1310","display_name":"Endocrinology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11916","display_name":"Legionella and Acanthamoeba research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1310","display_name":"Endocrinology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11688","display_name":"Microbial infections and disease research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12824","display_name":"Infections and bacterial resistance","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/legionella-pneumophila","display_name":"Legionella pneumophila","score":1.0},{"id":"https://openalex.org/keywords/virulence-genes","display_name":"virulence genes","score":0.9459999799728394},{"id":"https://openalex.org/keywords/dot-icm-type-iv-secretion-system","display_name":"Dot/Icm type IV secretion system","score":0.9010000228881836},{"id":"https://openalex.org/keywords/intracellular-replication","display_name":"intracellular replication","score":0.7440000176429749},{"id":"https://openalex.org/keywords/legionnaires-disease","display_name":"Legionnaires' disease","score":0.8180000185966492},{"id":"https://openalex.org/keywords/acanthamoeba-castellanii","display_name":"Acanthamoeba castellanii","score":0.8989999890327454},{"id":"https://openalex.org/keywords/transposon-mutants","display_name":"transposon mutants","score":0.8569999933242798},{"id":"https://openalex.org/keywords/legionella-containing-vacuole","display_name":"Legionella-containing vacuole","score":0.7929999828338623},{"id":"https://openalex.org/keywords/alveolar-macrophages","display_name":"alveolar macrophages","score":0.8240000009536743},{"id":"https://openalex.org/keywords/mouse-lung-infection","display_name":"mouse lung infection","score":0.42399999499320984}],"concepts":[{"id":"https://openalex.org/C2776118409","wikidata":"https://www.wikidata.org/wiki/Q147885","display_name":"Legionella pneumophila","level":3,"score":0.8611334562301636},{"id":"https://openalex.org/C60987743","wikidata":"https://www.wikidata.org/wiki/Q1460232","display_name":"Virulence","level":3,"score":0.7764050960540771},{"id":"https://openalex.org/C116834253","wikidata":"https://www.wikidata.org/wiki/Q2039217","display_name":"Identification (biology)","level":2,"score":0.7025991678237915},{"id":"https://openalex.org/C2779164008","wikidata":"https://www.wikidata.org/wiki/Q158013","display_name":"Legionella","level":3,"score":0.6401206254959106},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.5996838808059692},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4911305606365204},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.43886834383010864},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.37727177143096924},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.21460074186325073},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.16273069381713867},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.07630273699760437}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:jupiter.its.unimelb.edu.au:11343/56471","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/11343/56471","pdf_url":"http://hdl.handle.net/11343/56471","source":{"id":"https://openalex.org/S4377196259","display_name":"Minerva Access (University of Melbourne)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I165779595","host_organization_name":"The University of Melbourne","host_organization_lineage":["https://openalex.org/I165779595"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"PhD thesis"},{"id":"mag:2291225357","doi":null,"is_oa":false,"landing_page_url":"https://minerva-access.unimelb.edu.au/bitstream/11343/56471/1/VOGRIN-FinalThesis.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:jupiter.its.unimelb.edu.au:11343/56471","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/11343/56471","pdf_url":"http://hdl.handle.net/11343/56471","source":{"id":"https://openalex.org/S4377196259","display_name":"Minerva Access (University of Melbourne)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I165779595","host_organization_name":"The University of Melbourne","host_organization_lineage":["https://openalex.org/I165779595"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"PhD thesis"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.6521000266075134,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2291225357.pdf","grobid_xml":"https://content.openalex.org/works/W2291225357.grobid-xml"},"referenced_works_count":198,"referenced_works":["https://openalex.org/W53087725","https://openalex.org/W89674792","https://openalex.org/W168934269","https://openalex.org/W1501553911","https://openalex.org/W1503022370","https://openalex.org/W1522088230","https://openalex.org/W1558162216","https://openalex.org/W1595083713","https://openalex.org/W1597678601","https://openalex.org/W1676299908","https://openalex.org/W1765700753","https://openalex.org/W1783448612","https://openalex.org/W1790797992","https://openalex.org/W1936725904","https://openalex.org/W1947628375","https://openalex.org/W1948561455","https://openalex.org/W1964833882","https://openalex.org/W1965509064","https://openalex.org/W1966215744","https://openalex.org/W1966454211","https://openalex.org/W1966771851","https://openalex.org/W1971371415","https://openalex.org/W1972001431","https://openalex.org/W1973853916","https://openalex.org/W1974369116","https://openalex.org/W1975152145","https://openalex.org/W1976920903","https://openalex.org/W1977272060","https://openalex.org/W1978100320","https://openalex.org/W1978494873","https://openalex.org/W1980628868","https://openalex.org/W1981180892","https://openalex.org/W1981783746","https://openalex.org/W1982017960","https://openalex.org/W1983230305","https://openalex.org/W1984315016","https://openalex.org/W1987077730","https://openalex.org/W1988062115","https://openalex.org/W1989102699","https://openalex.org/W1992999376","https://openalex.org/W1994404141","https://openalex.org/W1997600163","https://openalex.org/W1999355125","https://openalex.org/W1999461765","https://openalex.org/W1999924463","https://openalex.org/W2000750186","https://openalex.org/W2001197606","https://openalex.org/W2004393319","https://openalex.org/W2004891282","https://openalex.org/W2005077686","https://openalex.org/W2005210354","https://openalex.org/W2006639171","https://openalex.org/W2007349675","https://openalex.org/W2008034134","https://openalex.org/W2008116827","https://openalex.org/W2009600027","https://openalex.org/W2011729533","https://openalex.org/W2012053032","https://openalex.org/W2013425197","https://openalex.org/W2014729052","https://openalex.org/W2016228685","https://openalex.org/W2019410617","https://openalex.org/W2020873236","https://openalex.org/W2021878136","https://openalex.org/W2023581910","https://openalex.org/W2023805832","https://openalex.org/W2023974021","https://openalex.org/W2024405559","https://openalex.org/W2024746774","https://openalex.org/W2027519905","https://openalex.org/W2027618375","https://openalex.org/W2029526066","https://openalex.org/W2031541480","https://openalex.org/W2033828066","https://openalex.org/W2034836766","https://openalex.org/W2035333716","https://openalex.org/W2036151553","https://openalex.org/W2036631152","https://openalex.org/W2036679605","https://openalex.org/W2037566791","https://openalex.org/W2039629288","https://openalex.org/W2040516599","https://openalex.org/W2040871602","https://openalex.org/W2043807816","https://openalex.org/W2044376487","https://openalex.org/W2045072282","https://openalex.org/W2047410466","https://openalex.org/W2050204065","https://openalex.org/W2050834086","https://openalex.org/W2052996021","https://openalex.org/W2056756764","https://openalex.org/W2058782620","https://openalex.org/W2060518890","https://openalex.org/W2060702462","https://openalex.org/W2062562477","https://openalex.org/W2063718050","https://openalex.org/W2063839610","https://openalex.org/W2066210800","https://openalex.org/W2066220084","https://openalex.org/W2066974855","https://openalex.org/W2067600997","https://openalex.org/W2070479570","https://openalex.org/W2070549262","https://openalex.org/W2071905511","https://openalex.org/W2073941299","https://openalex.org/W2076490464","https://openalex.org/W2077903776","https://openalex.org/W2079017226","https://openalex.org/W2079580539","https://openalex.org/W2080563387","https://openalex.org/W2081578405","https://openalex.org/W2082291851","https://openalex.org/W2084917885","https://openalex.org/W2085389244","https://openalex.org/W2085753387","https://openalex.org/W2086799637","https://openalex.org/W2087770859","https://openalex.org/W2089223791","https://openalex.org/W2091751687","https://openalex.org/W2091884933","https://openalex.org/W2092179351","https://openalex.org/W2094670332","https://openalex.org/W2096466814","https://openalex.org/W2097062479","https://openalex.org/W2097278543","https://openalex.org/W2097551453","https://openalex.org/W2097755834","https://openalex.org/W2098231476","https://openalex.org/W2100668965","https://openalex.org/W2103741616","https://openalex.org/W2105537192","https://openalex.org/W2105836254","https://openalex.org/W2107674270","https://openalex.org/W2108051298","https://openalex.org/W2108244474","https://openalex.org/W2108819945","https://openalex.org/W2109910885","https://openalex.org/W2111356152","https://openalex.org/W2111873576","https://openalex.org/W2114475032","https://openalex.org/W2114499229","https://openalex.org/W2115644464","https://openalex.org/W2116936521","https://openalex.org/W2118261313","https://openalex.org/W2118759489","https://openalex.org/W2119041176","https://openalex.org/W2120117120","https://openalex.org/W2122800099","https://openalex.org/W2124781105","https://openalex.org/W2126155394","https://openalex.org/W2127092072","https://openalex.org/W2128236754","https://openalex.org/W2129374544","https://openalex.org/W2130156413","https://openalex.org/W2131401167","https://openalex.org/W2133013758","https://openalex.org/W2133631293","https://openalex.org/W2135541591","https://openalex.org/W2136000113","https://openalex.org/W2136246773","https://openalex.org/W2136494401","https://openalex.org/W2137144854","https://openalex.org/W2141885858","https://openalex.org/W2144168531","https://openalex.org/W2145251715","https://openalex.org/W2145410393","https://openalex.org/W2147849194","https://openalex.org/W2149063136","https://openalex.org/W2150869803","https://openalex.org/W2151829625","https://openalex.org/W2153023449","https://openalex.org/W2153167722","https://openalex.org/W2153724411","https://openalex.org/W2154077252","https://openalex.org/W2154631013","https://openalex.org/W2155407905","https://openalex.org/W2155795106","https://openalex.org/W2156241274","https://openalex.org/W2157778652","https://openalex.org/W2158958374","https://openalex.org/W2160246570","https://openalex.org/W2161269876","https://openalex.org/W2163505606","https://openalex.org/W2163524709","https://openalex.org/W2164069152","https://openalex.org/W2164075646","https://openalex.org/W2164097345","https://openalex.org/W2165155657","https://openalex.org/W2165437729","https://openalex.org/W2165956093","https://openalex.org/W2168701991","https://openalex.org/W2170419834","https://openalex.org/W2170488656","https://openalex.org/W2213964329","https://openalex.org/W2310680479","https://openalex.org/W2398112608","https://openalex.org/W2460392402","https://openalex.org/W4236470188"],"related_works":["https://openalex.org/W2578422426","https://openalex.org/W7097675400","https://openalex.org/W7100694355","https://openalex.org/W2802329132","https://openalex.org/W7095811847","https://openalex.org/W2523437513","https://openalex.org/W2922177984","https://openalex.org/W4412366277","https://openalex.org/W2153023449","https://openalex.org/W4400180265"],"abstract_inverted_index":{"Legionella":[0,36],"pneumophila,":[1],"the":[2,7,35,43,59,63,80,87,122,135,160,172,187,192,204,210,216,227,233,244,267,286],"major":[3,98],"causative":[4],"agent":[5],"of":[6,65,111,130,159,174,196,246,259,269],"severely":[8],"pneumonic":[9],"Legionnaires'":[10],"disease,":[11],"is":[12,18,73],"an":[13,197,224],"intracellular":[14,101,119,211],"bacterial":[15,70],"pathogen":[16],"that":[17,68,95,203,279],"able":[19],"to":[20,55,58,142,249,284],"exploit":[21],"aquatic":[22],"protozoa":[23],"as":[24,26,30],"well":[25],"human":[27],"alveolar":[28],"macrophages":[29,264],"hosts":[31],"for":[32,117,186,191,215,226,232,256,289],"replication.After":[33],"internalisation,":[34],"containing":[37],"vacuole":[38,67],"(LCV)":[39],"avoids":[40],"fusion":[41],"with":[42,275,293],"endosomal":[44],"pathway":[45],"and":[46,72,107,153,180,241,265],"intercepts":[47],"endoplasmic":[48],"reticulum":[49],"(ER)-derived":[50],"early":[51],"secretory":[52],"vesicles":[53],"prior":[54],"their":[56,290],"transport":[57],"Golgi.This":[60],"aids":[61],"in":[62,100,167,262,266],"formation":[64],"a":[66,97,109,156,168,237],"supports":[69],"replication":[71,120,188,212,228,258],"driven":[74],"by":[75,79],"effector":[76,240],"proteins":[77],"translocated":[78,239],"Dot/Icm":[81,88,136,238],"type":[82],"IV":[83,138],"secretion":[84,139],"system.Apart":[85],"from":[86],"system,":[89],"few":[90],"genes":[91,148],"have":[92],"been":[93],"identified":[94,166,175],"play":[96],"role":[99],"replication.In":[102],"this":[103,247,294],"study,":[104],"we":[105,242],"constructed":[106],"screened":[108],"library":[110],"10,006":[112],"L.":[113,250,260],"pneumophila":[114,251,261],"transposon":[115,193,217,234],"mutants":[116,126],"attenuated":[118],"within":[121],"amoebae":[123],"Acanthamoeba":[124],"castellanii.34":[125],"were":[127,149,165,177],"identified,":[128],"21":[129],"which":[131],"carried":[132],"insertions":[133,144],"into":[134,145],"Type":[137],"system.In":[140],"addition":[141],"these,":[143],"seven":[146],"novel":[147],"discovered,":[150],"including":[151],"lpw27511":[152,199],"sdeC.lpw27511":[154],"encodes":[155,236],"transcriptional":[157],"regulator":[158],"LuxR":[161],"family.Its":[162],"regulatory":[163],"targets":[164,176],"DNA":[169],"microarray,":[170],"however,":[171],"majority":[173],"weakly":[178],"affected":[179],"there":[181],"was":[182,254],"no":[183],"obvious":[184],"explanation":[185,225],"defect":[189,213,229],"observed":[190,231],"mutant.Further":[194],"analysis":[195],"in-frame":[198],"deletion":[200],"mutant":[201,206],"showed":[202,278],"defined":[205],"did":[207],"not":[208,222],"show":[209],"seen":[214],"mutant.Unfortunately":[218],"subsequent":[219],"investigations":[220],"could":[221],"find":[223],"originally":[230],"mutant.sdeC":[235],"investigated":[243],"contribution":[245],"protein":[248],"pathogenesis":[252],"further.sdeC":[253],"required":[255],"full":[257],"amoebae,":[263],"lungs":[268],"A":[270],"strain":[271],"mice.HEK293T":[272],"cells":[273],"transfected":[274],"GFP-tagged":[276],"SdeC":[277,280],"co-localised":[281],"I":[282],"wish":[283],"acknowledge":[285],"following":[287],"people":[288],"invaluable":[291],"assistance":[292],"study.":[295]},"counts_by_year":[],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2016-06-24T00:00:00"}