{"id":"https://openalex.org/W2289453066","doi":"https://doi.org/10.1016/j.jviromet.2016.02.011","title":"Scalable chromatography-based purification of virus-like particle carrier for epitope based influenza A vaccine produced in Escherichia coli","display_name":"Scalable chromatography-based purification of virus-like particle carrier for epitope based influenza A vaccine produced in Escherichia coli","publication_year":2016,"publication_date":"2016-03-05","ids":{"openalex":"https://openalex.org/W2289453066","doi":"https://doi.org/10.1016/j.jviromet.2016.02.011","mag":"2289453066","pmid":"https://pubmed.ncbi.nlm.nih.gov/26947397"},"language":"en","primary_location":{"id":"doi:10.1016/j.jviromet.2016.02.011","doi":"https://doi.org/10.1016/j.jviromet.2016.02.011","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.jviromet.2016.02.011","pdf_url":null,"source":{"id":"https://openalex.org/S7140881","display_name":"Journal of Virological Methods","issn_l":"0166-0934","issn":["0166-0934","1879-0984"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Virological Methods","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5080311405","display_name":"Priscillia Lagoutte","orcid":"https://orcid.org/0000-0002-5117-820X","observed_orcids":["https://orcid.org/0000-0002-5117-820X"]},"institutions":[{"id":"https://openalex.org/I4210140983","display_name":"BIOASTER","ror":"https://ror.org/04awzyg03","country_code":"FR","type":"nonprofit","lineage":["https://openalex.org/I4210140983"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Priscillia Lagoutte","raw_affiliation_strings":["BIOASTER, Protein and Expression System Engineering unit, 40 avenue Tony Garnier, 69007 Lyon, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"BIOASTER, Protein and Expression System Engineering unit, 40 avenue Tony Garnier, 69007 Lyon, France","institution_ids":["https://openalex.org/I4210140983"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018365507","display_name":"Charlotte Mignon","orcid":"https://orcid.org/0009-0001-1725-5339","observed_orcids":["https://orcid.org/0009-0001-1725-5339"]},"institutions":[{"id":"https://openalex.org/I4210140983","display_name":"BIOASTER","ror":"https://ror.org/04awzyg03","country_code":"FR","type":"nonprofit","lineage":["https://openalex.org/I4210140983"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Charlotte Mignon","raw_affiliation_strings":["BIOASTER, Protein and Expression System Engineering unit, 40 avenue Tony Garnier, 69007 Lyon, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"BIOASTER, Protein and Expression System Engineering unit, 40 avenue Tony Garnier, 69007 Lyon, France","institution_ids":["https://openalex.org/I4210140983"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5047295527","display_name":"St\u00e9phanie Donnat","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210140983","display_name":"BIOASTER","ror":"https://ror.org/04awzyg03","country_code":"FR","type":"nonprofit","lineage":["https://openalex.org/I4210140983"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"St\u00e9phanie Donnat","raw_affiliation_strings":["BIOASTER, Protein and Expression System Engineering unit, 40 avenue Tony Garnier, 69007 Lyon, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"BIOASTER, Protein and Expression System Engineering unit, 40 avenue Tony Garnier, 69007 Lyon, France","institution_ids":["https://openalex.org/I4210140983"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5019085817","display_name":"Gustavo Stadthagen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210140983","display_name":"BIOASTER","ror":"https://ror.org/04awzyg03","country_code":"FR","type":"nonprofit","lineage":["https://openalex.org/I4210140983"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Gustavo Stadthagen","raw_affiliation_strings":["BIOASTER, Protein and Expression System Engineering unit, 40 avenue Tony Garnier, 69007 Lyon, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"BIOASTER, Protein and Expression System Engineering unit, 40 avenue Tony Garnier, 69007 Lyon, France","institution_ids":["https://openalex.org/I4210140983"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5061324725","display_name":"Jan Mast","orcid":"https://orcid.org/0000-0002-4879-3367","observed_orcids":["https://orcid.org/0000-0002-4879-3367"]},"institutions":[{"id":"https://openalex.org/I4210108698","display_name":"Veterinary and Agrochemical Research Centre","ror":"https://ror.org/0153sam53","country_code":"BE","type":"facility","lineage":["https://openalex.org/I4210108698"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"Jan Mast","raw_affiliation_strings":["EM Service, CODA-CERVA, Groeselenbergstraat 99, B-1180 Brussels, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"EM Service, CODA-CERVA, Groeselenbergstraat 99, B-1180 Brussels, Belgium","institution_ids":["https://openalex.org/I4210108698"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108468319","display_name":"R\u00e9gis Sodoyer","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210140983","display_name":"BIOASTER","ror":"https://ror.org/04awzyg03","country_code":"FR","type":"nonprofit","lineage":["https://openalex.org/I4210140983"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"R\u00e9gis Sodoyer","raw_affiliation_strings":["BIOASTER, Protein and Expression System Engineering unit, 40 avenue Tony Garnier, 69007 Lyon, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"BIOASTER, Protein and Expression System Engineering unit, 40 avenue Tony Garnier, 69007 Lyon, France","institution_ids":["https://openalex.org/I4210140983"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5070907593","display_name":"Adrien Lugari","orcid":"https://orcid.org/0000-0001-8337-6043","observed_orcids":["https://orcid.org/0000-0001-8337-6043"]},"institutions":[{"id":"https://openalex.org/I4210140983","display_name":"BIOASTER","ror":"https://ror.org/04awzyg03","country_code":"FR","type":"nonprofit","lineage":["https://openalex.org/I4210140983"]}],"countries":["FR"],"is_corresponding":true,"raw_author_name":"Adrien Lugari","raw_affiliation_strings":["BIOASTER, Protein and Expression System Engineering unit, 40 avenue Tony Garnier, 69007 Lyon, France. Electronic address: adrien.lugari@bioaster.org","BIOASTER, Protein and Expression System Engineering unit, 40 avenue Tony Garnier, 69007 Lyon, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"BIOASTER, Protein and Expression System Engineering unit, 40 avenue Tony Garnier, 69007 Lyon, France. Electronic address: adrien.lugari@bioaster.org","institution_ids":["https://openalex.org/I4210140983"]},{"raw_affiliation_string":"BIOASTER, Protein and Expression System Engineering unit, 40 avenue Tony Garnier, 69007 Lyon, France","institution_ids":["https://openalex.org/I4210140983"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5029588569","display_name":"Bettina Werle","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210140983","display_name":"BIOASTER","ror":"https://ror.org/04awzyg03","country_code":"FR","type":"nonprofit","lineage":["https://openalex.org/I4210140983"]}],"countries":["FR"],"is_corresponding":true,"raw_author_name":"Bettina Werle","raw_affiliation_strings":["BIOASTER, Protein and Expression System Engineering unit, 40 avenue Tony Garnier, 69007 Lyon, France. Electronic address: bettina.werle@bioaster.org","BIOASTER, Protein and Expression System Engineering unit, 40 avenue Tony Garnier, 69007 Lyon, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"BIOASTER, Protein and Expression System Engineering unit, 40 avenue Tony Garnier, 69007 Lyon, France. Electronic address: bettina.werle@bioaster.org","institution_ids":["https://openalex.org/I4210140983"]},{"raw_affiliation_string":"BIOASTER, Protein and Expression System Engineering unit, 40 avenue Tony Garnier, 69007 Lyon, France","institution_ids":["https://openalex.org/I4210140983"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5029588569","https://openalex.org/A5070907593"],"corresponding_institution_ids":["https://openalex.org/I4210140983"],"apc_list":{"value":3210,"currency":"USD","value_usd":3210},"apc_paid":null,"fwci":2.0209,"has_fulltext":false,"cited_by_count":58,"citation_normalized_percentile":{"value":0.86697935,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":99},"biblio":{"volume":"232","issue":null,"first_page":"8","last_page":"11"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.9961000084877014,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.9961000084877014,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10167","display_name":"Influenza Virus Research Studies","score":0.00139999995008111,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.0010000000474974513,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/virus-like-particles","display_name":"virus-like particles","score":0.9850000143051147},{"id":"https://openalex.org/keywords/influenza-a-vaccine","display_name":"influenza A vaccine","score":0.9829999804496765},{"id":"https://openalex.org/keywords/epitope-based-vaccine","display_name":"epitope-based vaccine","score":0.8090000152587891},{"id":"https://openalex.org/keywords/size-exclusion-chromatography","display_name":"size exclusion chromatography","score":0.8190000057220459},{"id":"https://openalex.org/keywords/vlp-purification","display_name":"VLP purification","score":0.6899999976158142},{"id":"https://openalex.org/keywords/escherichia-coli-expression","display_name":"Escherichia coli expression","score":0.8379999995231628},{"id":"https://openalex.org/keywords/bacteriophage-ms2","display_name":"bacteriophage MS2","score":0.8519999980926514},{"id":"https://openalex.org/keywords/detergent-extraction","display_name":"detergent extraction","score":0.7170000076293945},{"id":"https://openalex.org/keywords/downstream-processing","display_name":"downstream processing","score":0.531000018119812},{"id":"https://openalex.org/keywords/vaccine-production","display_name":"vaccine production","score":0.6520000100135803},{"id":"https://openalex.org/keywords/veterinary-vaccines","display_name":"veterinary vaccines","score":0.7409999966621399}],"concepts":[{"id":"https://openalex.org/C2776387124","wikidata":"https://www.wikidata.org/wiki/Q1475781","display_name":"Virus-like particle","level":4,"score":0.7899324893951416},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6483726501464844},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.6248384714126587},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.6158168911933899},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.5769542455673218},{"id":"https://openalex.org/C195616568","wikidata":"https://www.wikidata.org/wiki/Q128711","display_name":"Epitope","level":3,"score":0.5510254502296448},{"id":"https://openalex.org/C67568554","wikidata":"https://www.wikidata.org/wiki/Q385442","display_name":"Downstream processing","level":2,"score":0.5150815844535828},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.49070680141448975},{"id":"https://openalex.org/C18743360","wikidata":"https://www.wikidata.org/wiki/Q1208096","display_name":"Diafiltration","level":4,"score":0.45064857602119446},{"id":"https://openalex.org/C179247698","wikidata":"https://www.wikidata.org/wiki/Q382799","display_name":"Affinity chromatography","level":3,"score":0.41631385684013367},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.16595500707626343},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.15383273363113403},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.15381336212158203},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.0},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.0},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.0},{"id":"https://openalex.org/C48314217","wikidata":"https://www.wikidata.org/wiki/Q1753040","display_name":"Microfiltration","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D000086082","descriptor_name":"Viroporin Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000939","descriptor_name":"Epitopes","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000939","descriptor_name":"Epitopes","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D002850","descriptor_name":"Chromatography, Gel","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007252","descriptor_name":"Influenza Vaccines","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D007252","descriptor_name":"Influenza Vaccines","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D009976","descriptor_name":"Orthomyxoviridae Infections","qualifier_ui":"Q000517","qualifier_name":"prevention & control","is_major_topic":false},{"descriptor_ui":"D009976","descriptor_name":"Orthomyxoviridae Infections","qualifier_ui":"Q000662","qualifier_name":"veterinary","is_major_topic":false},{"descriptor_ui":"D009980","descriptor_name":"Influenza A virus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D009980","descriptor_name":"Influenza A virus","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D014614","descriptor_name":"Vaccines, Synthetic","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D014614","descriptor_name":"Vaccines, Synthetic","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D014730","descriptor_name":"Veterinary Medicine","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D014763","descriptor_name":"Viral Matrix Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D014763","descriptor_name":"Viral Matrix Proteins","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D017909","descriptor_name":"Levivirus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D058425","descriptor_name":"Vaccines, Virus-Like Particle","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D058425","descriptor_name":"Vaccines, Virus-Like Particle","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1016/j.jviromet.2016.02.011","doi":"https://doi.org/10.1016/j.jviromet.2016.02.011","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.jviromet.2016.02.011","pdf_url":null,"source":{"id":"https://openalex.org/S7140881","display_name":"Journal of Virological Methods","issn_l":"0166-0934","issn":["0166-0934","1879-0984"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Virological Methods","raw_type":"journal-article"},{"id":"pmid:26947397","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/26947397","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of virological methods","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.8048999905586243}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":29,"referenced_works":["https://openalex.org/W1949772429","https://openalex.org/W1967781933","https://openalex.org/W1973540262","https://openalex.org/W1978401933","https://openalex.org/W1991701295","https://openalex.org/W1999106655","https://openalex.org/W2003413689","https://openalex.org/W2005014362","https://openalex.org/W2007816485","https://openalex.org/W2035851795","https://openalex.org/W2039048600","https://openalex.org/W2054243692","https://openalex.org/W2056913645","https://openalex.org/W2060807469","https://openalex.org/W2066007237","https://openalex.org/W2072698306","https://openalex.org/W2074312008","https://openalex.org/W2081983857","https://openalex.org/W2107930497","https://openalex.org/W2120321866","https://openalex.org/W2129178711","https://openalex.org/W2139053022","https://openalex.org/W2145457261","https://openalex.org/W2146700406","https://openalex.org/W2147026505","https://openalex.org/W2149913957","https://openalex.org/W2151583667","https://openalex.org/W2161609692","https://openalex.org/W4210960157"],"related_works":["https://openalex.org/W2774749710","https://openalex.org/W6921042286","https://openalex.org/W2126013345","https://openalex.org/W2417585619","https://openalex.org/W2003004506","https://openalex.org/W2129178711","https://openalex.org/W2898596097","https://openalex.org/W2763475437","https://openalex.org/W2617521955","https://openalex.org/W2040211968"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":5},{"year":2025,"cited_by_count":7},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":8},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":6},{"year":2020,"cited_by_count":8},{"year":2019,"cited_by_count":9},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":4}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}