{"id":"https://openalex.org/W2287665703","doi":null,"title":"Investigation of Protein and Inhibitor Binding to O-Acetylpeptidoglycan Esterase 1 from Neisseria gonorrhoeae","display_name":"Investigation of Protein and Inhibitor Binding to O-Acetylpeptidoglycan Esterase 1 from Neisseria gonorrhoeae","publication_year":2015,"publication_date":"2015-10-09","ids":{"openalex":"https://openalex.org/W2287665703","mag":"2287665703"},"language":"en","primary_location":{"id":"pmh:oai:atrium.lib.uoguelph.ca:10214/9293","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10214/9293","pdf_url":"http://atrium.lib.uoguelph.ca/xmlui/bitstream/10214/9293/1/Ecclestone_Mark_201510_Msc.pdf","source":{"id":"https://openalex.org/S4306401043","display_name":"The Atrium (University of Guelph)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I79817857","host_organization_name":"University of Guelph","host_organization_lineage":["https://openalex.org/I79817857"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://atrium.lib.uoguelph.ca/xmlui/bitstream/10214/9293/1/Ecclestone_Mark_201510_Msc.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5054457043","display_name":"Mark Ecclestone","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Mark Ecclestone","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5054457043"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9092000126838684,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9092000126838684,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10147","display_name":"Antibiotic Resistance in Bacteria","score":0.04729999974370003,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":0.006200000178068876,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/neisseria-gonorrhoeae","display_name":"Neisseria gonorrhoeae","score":0.9639999866485596},{"id":"https://openalex.org/keywords/purpurin","display_name":"purpurin","score":0.8479999899864197},{"id":"https://openalex.org/keywords/alizarin","display_name":"alizarin","score":0.8230000138282776},{"id":"https://openalex.org/keywords/multienzyme-complex","display_name":"multienzyme complex","score":0.8519999980926514},{"id":"https://openalex.org/keywords/peptidoglycan-turnover","display_name":"peptidoglycan turnover","score":0.9399999976158142},{"id":"https://openalex.org/keywords/lytic-transglycosylase","display_name":"lytic transglycosylase","score":0.9269999861717224},{"id":"https://openalex.org/keywords/aminoglycoside-synergy","display_name":"aminoglycoside synergy","score":0.890999972820282},{"id":"https://openalex.org/keywords/bacillus-cereus","display_name":"Bacillus cereus","score":0.9769999980926514},{"id":"https://openalex.org/keywords/protein-ligand-binding","display_name":"protein-ligand binding","score":0.6940000057220459}],"concepts":[{"id":"https://openalex.org/C2776634448","wikidata":"https://www.wikidata.org/wiki/Q206920","display_name":"Peptidoglycan","level":3,"score":0.9117031693458557},{"id":"https://openalex.org/C2777261171","wikidata":"https://www.wikidata.org/wiki/Q418750","display_name":"Esterase","level":3,"score":0.6798752546310425},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6607183814048767},{"id":"https://openalex.org/C2779166590","wikidata":"https://www.wikidata.org/wiki/Q131129","display_name":"Neisseria gonorrhoeae","level":2,"score":0.5621523261070251},{"id":"https://openalex.org/C201663137","wikidata":"https://www.wikidata.org/wiki/Q21198990","display_name":"Periplasmic space","level":4,"score":0.5067091584205627},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.4844382107257843},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.4511338174343109},{"id":"https://openalex.org/C2777118263","wikidata":"https://www.wikidata.org/wiki/Q131307","display_name":"Bacillus cereus","level":3,"score":0.44794347882270813},{"id":"https://openalex.org/C2780867653","wikidata":"https://www.wikidata.org/wiki/Q574302","display_name":"Anthraquinones","level":2,"score":0.42390909790992737},{"id":"https://openalex.org/C145770059","wikidata":"https://www.wikidata.org/wiki/Q751908","display_name":"Catalytic triad","level":4,"score":0.418631911277771},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3942251205444336},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3924437165260315},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.30441492795944214},{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.22716394066810608},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.08695781230926514},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:atrium.lib.uoguelph.ca:10214/9293","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10214/9293","pdf_url":"http://atrium.lib.uoguelph.ca/xmlui/bitstream/10214/9293/1/Ecclestone_Mark_201510_Msc.pdf","source":{"id":"https://openalex.org/S4306401043","display_name":"The Atrium (University of Guelph)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I79817857","host_organization_name":"University of Guelph","host_organization_lineage":["https://openalex.org/I79817857"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},{"id":"mag:2287665703","doi":null,"is_oa":false,"landing_page_url":"https://atrium.lib.uoguelph.ca/xmlui/bitstream/handle/10214/9293/Ecclestone_Mark_201510_Msc.pdf;sequence=1","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:atrium.lib.uoguelph.ca:10214/9293","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10214/9293","pdf_url":"http://atrium.lib.uoguelph.ca/xmlui/bitstream/10214/9293/1/Ecclestone_Mark_201510_Msc.pdf","source":{"id":"https://openalex.org/S4306401043","display_name":"The Atrium (University of Guelph)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I79817857","host_organization_name":"University of Guelph","host_organization_lineage":["https://openalex.org/I79817857"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2287665703.pdf","grobid_xml":"https://content.openalex.org/works/W2287665703.grobid-xml"},"referenced_works_count":79,"referenced_works":["https://openalex.org/W2372988","https://openalex.org/W159235474","https://openalex.org/W1502692131","https://openalex.org/W1511030774","https://openalex.org/W1523447849","https://openalex.org/W1893905942","https://openalex.org/W1921782533","https://openalex.org/W1962852480","https://openalex.org/W1964499067","https://openalex.org/W1968610738","https://openalex.org/W1978514212","https://openalex.org/W1980705976","https://openalex.org/W1981619865","https://openalex.org/W1988775133","https://openalex.org/W1989171083","https://openalex.org/W1989966734","https://openalex.org/W1990043420","https://openalex.org/W2006387962","https://openalex.org/W2007163142","https://openalex.org/W2012526340","https://openalex.org/W2026442758","https://openalex.org/W2029562819","https://openalex.org/W2035728933","https://openalex.org/W2036054788","https://openalex.org/W2036574878","https://openalex.org/W2040895520","https://openalex.org/W2044562813","https://openalex.org/W2045404157","https://openalex.org/W2049395582","https://openalex.org/W2050523158","https://openalex.org/W2052779789","https://openalex.org/W2056014377","https://openalex.org/W2061953031","https://openalex.org/W2062082885","https://openalex.org/W2064358027","https://openalex.org/W2064573072","https://openalex.org/W2065311355","https://openalex.org/W2066480710","https://openalex.org/W2069295776","https://openalex.org/W2073950027","https://openalex.org/W2077225701","https://openalex.org/W2077358324","https://openalex.org/W2077914268","https://openalex.org/W2078064634","https://openalex.org/W2079740756","https://openalex.org/W2085536363","https://openalex.org/W2085593604","https://openalex.org/W2086806574","https://openalex.org/W2091367128","https://openalex.org/W2092788509","https://openalex.org/W2097067733","https://openalex.org/W2097402634","https://openalex.org/W2101108802","https://openalex.org/W2101809821","https://openalex.org/W2102153654","https://openalex.org/W2103072608","https://openalex.org/W2103301321","https://openalex.org/W2105472305","https://openalex.org/W2108588612","https://openalex.org/W2112580008","https://openalex.org/W2118761676","https://openalex.org/W2119711877","https://openalex.org/W2120673715","https://openalex.org/W2127893070","https://openalex.org/W2135301585","https://openalex.org/W2139532707","https://openalex.org/W2141099568","https://openalex.org/W2143867657","https://openalex.org/W2144337101","https://openalex.org/W2150568514","https://openalex.org/W2152316711","https://openalex.org/W2157719203","https://openalex.org/W2159131031","https://openalex.org/W2165647053","https://openalex.org/W2166450440","https://openalex.org/W2168452238","https://openalex.org/W2541785787","https://openalex.org/W2593672338","https://openalex.org/W2739853558"],"related_works":["https://openalex.org/W2541785787","https://openalex.org/W2007163142","https://openalex.org/W7052340328","https://openalex.org/W1989171083","https://openalex.org/W2593672338","https://openalex.org/W7220834694","https://openalex.org/W4366131934","https://openalex.org/W2313511418","https://openalex.org/W2077358324","https://openalex.org/W2315276711"],"abstract_inverted_index":{"O-Acetylpeptidoglycan":[0],"esterase":[1,7,85],"1":[2],"(Ape1)":[3],"is":[4,48],"a":[5,18,109,130],"periplasmic":[6],"present":[8],"in":[9,21,89,108],"pathogenic":[10],"organisms":[11],"that":[12,97,103],"produce":[13,98],"O-acetylated":[14],"peptidoglycan.":[15,44],"Ape1a":[16,46],"plays":[17],"crucial":[19],"role":[20],"the":[22,29,37,42,54,65,72,84,93],"growth":[23,94],"of":[24,31,36,41,67,75,87,95,116,133],"these":[25],"bacteria":[26,96],"by":[27,53,64],"regulating":[28],"turnover":[30,52],"peptidoglycan":[32,51],"through":[33],"catalytic":[34],"removal":[35],"C-6":[38,73],"acetyl":[39,69],"group":[40,70,115],"modified":[43],"Specifically,":[45],"activity":[47,86],"necessary":[49],"for":[50],"main":[55],"autolytic":[56],"enzymes,":[57],"lytic":[58],"transglycosylases":[59],"(LTs);":[60],"LTs":[61],"are":[62],"blocked":[63],"presence":[66],"an":[68],"on":[71],"hydroxyl":[74],"N-acetylmuramic":[76],"acid.":[77],"Purpurin":[78,135],"has":[79],"been":[80],"observed":[81],"to":[82,106,124,127,138],"inhibit":[83],"Ape1":[88,104],"vitro":[90],"and":[91],"inhibits":[92],"O-acetylpeptidoglycan.":[99],"This":[100],"study":[101],"found":[102],"binds":[105],"FtsZ":[107],"presumed":[110],"multi-enzyme":[111],"complex.":[112],"The":[113],"2-hydroxyl":[114],"anthraquinones,":[117],"such":[118],"as":[119,129],"purpurin":[120],"or":[121],"alizarin,":[122],"needs":[123],"be":[125],"deprotonated":[126],"work":[128,139],"suitable":[131],"inhibitor":[132],"Ape1.":[134],"was":[136],"demonstrated":[137],"synergistically":[140],"with":[141],"aminoglycosides":[142],"against":[143],"Bacillus":[144],"cereus":[145],"species.":[146]},"counts_by_year":[],"updated_date":"2026-08-21T09:56:20.448147","created_date":"2016-06-24T00:00:00"}