{"id":"https://openalex.org/W2286273154","doi":null,"title":"PRODUCTION AND FUNCTIONAL ANALYSIS OF RECOMBINANT BOVINE MORPHOGENIC PROTEIN 15 A THESIS","display_name":"PRODUCTION AND FUNCTIONAL ANALYSIS OF RECOMBINANT BOVINE MORPHOGENIC PROTEIN 15 A THESIS","publication_year":2013,"publication_date":"2013-01-01","ids":{"openalex":"https://openalex.org/W2286273154","mag":"2286273154"},"language":"en","primary_location":{"id":"pmh:oai:oaktrust.library.tamu.edu:1969.1/151804","doi":null,"is_oa":false,"landing_page_url":"https://oaktrust.library.tamu.edu/bitstreams/a6b30369-0c53-44cf-93f3-bcfa939f0c3c/download","pdf_url":null,"source":{"id":"https://openalex.org/S4306400291","display_name":"OakTrust (Texas A&M University Libraries)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I91045830","host_organization_name":"Texas A&M University","host_organization_lineage":["https://openalex.org/I91045830"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5056559576","display_name":"Gregory Willis Burns","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Gregory Willis Burns","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5056559576"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10364","display_name":"Reproductive Biology and Fertility","score":0.8666999936103821,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10364","display_name":"Reproductive Biology and Fertility","score":0.8666999936103821,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12929","display_name":"Animal Genetics and Reproduction","score":0.06270000338554382,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.01850000023841858,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bmp15","display_name":"BMP15","score":0.8220000267028809},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.796999990940094},{"id":"https://openalex.org/keywords/bovine-oocyte-maturation","display_name":"bovine oocyte maturation","score":0.8510000109672546},{"id":"https://openalex.org/keywords/in-vitro-embryo-production","display_name":"in vitro embryo production","score":0.8489999771118164},{"id":"https://openalex.org/keywords/oocyte-secreted-factors","display_name":"oocyte-secreted factors","score":0.800000011920929},{"id":"https://openalex.org/keywords/smad1-5-phosphorylation","display_name":"Smad1/5 phosphorylation","score":0.9409999847412109},{"id":"https://openalex.org/keywords/compound-c","display_name":"compound C","score":0.8209999799728394},{"id":"https://openalex.org/keywords/cyclooxygenase-2","display_name":"cyclooxygenase-2","score":0.6539999842643738},{"id":"https://openalex.org/keywords/tsg-6","display_name":"TSG-6","score":0.9900000095367432},{"id":"https://openalex.org/keywords/cumulus-oocyte-complexes","display_name":"cumulus-oocyte complexes","score":0.6169999837875366},{"id":"https://openalex.org/keywords/bovine-ovarian-tissue","display_name":"bovine ovarian tissue","score":0.4909999966621399},{"id":"https://openalex.org/keywords/post-translational-glycosylation","display_name":"post-translational glycosylation","score":0.7680000066757202}],"concepts":[{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.6338835954666138},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.37536340951919556},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3686178922653198},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2945360839366913},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.18185827136039734}],"mesh":[],"locations_count":3,"locations":[{"id":"pmh:oai:oaktrust.library.tamu.edu:1969.1/151804","doi":null,"is_oa":false,"landing_page_url":"https://oaktrust.library.tamu.edu/bitstreams/a6b30369-0c53-44cf-93f3-bcfa939f0c3c/download","pdf_url":null,"source":{"id":"https://openalex.org/S4306400291","display_name":"OakTrust (Texas A&M University Libraries)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I91045830","host_organization_name":"Texas A&M University","host_organization_lineage":["https://openalex.org/I91045830"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},{"id":"mag:2286273154","doi":null,"is_oa":false,"landing_page_url":"http://oaktrust.library.tamu.edu/bitstream/1969.1/151804/1/BURNS-THESIS-2013.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null},{"id":"mag:2496227372","doi":null,"is_oa":false,"landing_page_url":"http://oaktrust.library.tamu.edu/bitstream/handle/1969.1/151804/BURNS-THESIS-2013.pdf;sequence=1","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":110,"referenced_works":["https://openalex.org/W142386558","https://openalex.org/W613186813","https://openalex.org/W1488722406","https://openalex.org/W1501791679","https://openalex.org/W1510428462","https://openalex.org/W1540418377","https://openalex.org/W1563690189","https://openalex.org/W1618744733","https://openalex.org/W1639179179","https://openalex.org/W1922816797","https://openalex.org/W1964564069","https://openalex.org/W1968945784","https://openalex.org/W1969640830","https://openalex.org/W1973790057","https://openalex.org/W1974832873","https://openalex.org/W1975317517","https://openalex.org/W1978570830","https://openalex.org/W1978959968","https://openalex.org/W1980645073","https://openalex.org/W1981794598","https://openalex.org/W1983076004","https://openalex.org/W1986193633","https://openalex.org/W1987909360","https://openalex.org/W1990815117","https://openalex.org/W1993410552","https://openalex.org/W1996439082","https://openalex.org/W1996938657","https://openalex.org/W1997305195","https://openalex.org/W2002041730","https://openalex.org/W2005301046","https://openalex.org/W2007796762","https://openalex.org/W2009929121","https://openalex.org/W2010637356","https://openalex.org/W2013961452","https://openalex.org/W2018831893","https://openalex.org/W2023425928","https://openalex.org/W2027467068","https://openalex.org/W2028062152","https://openalex.org/W2030766017","https://openalex.org/W2031133866","https://openalex.org/W2035316898","https://openalex.org/W2039144927","https://openalex.org/W2042114928","https://openalex.org/W2043850967","https://openalex.org/W2045348184","https://openalex.org/W2048584018","https://openalex.org/W2051038137","https://openalex.org/W2056074333","https://openalex.org/W2056511007","https://openalex.org/W2058530663","https://openalex.org/W2058732512","https://openalex.org/W2060515743","https://openalex.org/W2062360693","https://openalex.org/W2062678765","https://openalex.org/W2072203210","https://openalex.org/W2072439295","https://openalex.org/W2073201349","https://openalex.org/W2075067274","https://openalex.org/W2076025380","https://openalex.org/W2076853816","https://openalex.org/W2081637758","https://openalex.org/W2082280903","https://openalex.org/W2083070222","https://openalex.org/W2085684986","https://openalex.org/W2088040832","https://openalex.org/W2089226493","https://openalex.org/W2090183771","https://openalex.org/W2091100378","https://openalex.org/W2093398099","https://openalex.org/W2093467583","https://openalex.org/W2093559962","https://openalex.org/W2093779955","https://openalex.org/W2099068198","https://openalex.org/W2100729936","https://openalex.org/W2109449272","https://openalex.org/W2111508487","https://openalex.org/W2115410748","https://openalex.org/W2116591857","https://openalex.org/W2118162811","https://openalex.org/W2118419827","https://openalex.org/W2124006685","https://openalex.org/W2124506012","https://openalex.org/W2124838379","https://openalex.org/W2126647613","https://openalex.org/W2126722856","https://openalex.org/W2126853519","https://openalex.org/W2130047944","https://openalex.org/W2134590344","https://openalex.org/W2135104064","https://openalex.org/W2139498893","https://openalex.org/W2141427087","https://openalex.org/W2146984594","https://openalex.org/W2149237119","https://openalex.org/W2150083639","https://openalex.org/W2152708819","https://openalex.org/W2153323265","https://openalex.org/W2154128645","https://openalex.org/W2156449575","https://openalex.org/W2164538541","https://openalex.org/W2166161256","https://openalex.org/W2168018194","https://openalex.org/W2168847939","https://openalex.org/W2172867362","https://openalex.org/W2180160894","https://openalex.org/W2199021400","https://openalex.org/W2257417852","https://openalex.org/W2284316917","https://openalex.org/W2313466845","https://openalex.org/W2400698383","https://openalex.org/W2416967550"],"related_works":["https://openalex.org/W2013722372","https://openalex.org/W2164538541","https://openalex.org/W2121685422","https://openalex.org/W4251056382","https://openalex.org/W349802385","https://openalex.org/W2002041730","https://openalex.org/W2057651459","https://openalex.org/W1998689661","https://openalex.org/W2391798062","https://openalex.org/W2013961452"],"abstract_inverted_index":{"After":[0],"40":[1],"years":[2],"of":[3,38,60,69,82,111,169,185,192,231,245,271,292,311,346,352,378,391],"research,":[4],"in":[5,20,28,208,228,283,289,322,353,395],"vitro":[6,29,229,284,354,396],"systems":[7,33],"for":[8,189,237,286,325,388],"mammalian":[9,142],"embryo":[10],"production":[11],"produce":[12],"lower":[13],"quality":[14],"embryos":[15],"than":[16,78],"those":[17],"derived":[18],"from":[19,35,48,99,102,171,200,300],"vivo":[21],"sources.":[22,50],"Recent":[23],"reports":[24],"have":[25],"demonstrated":[26,259],"that":[27,67],"bovine":[30,91,103,153,210,303,393],"oocyte":[31,39,71,277,280,357,394],"maturation":[32],"benefit":[34],"the":[36,79,112,126,139,183,201,238,260,269,290,349,376,389],"addition":[37,377],"secreted":[40,72],"factors,":[41],"specifically":[42],"Bone":[43],"Morphogenic":[44],"Protein":[45],"15":[46],"(BMP15)":[47],"heterologous":[49,83],"However,":[51],"known":[52],"amino":[53],"acid":[54],"sequence":[55,121],"variation":[56],"and":[57,109,150,161,167,182,195,220,234,316,372],"species-specific":[58],"patterns":[59],"post-translational":[61],"glycosylation":[62],"lead":[63],"us":[64],"to":[65,164,341,380],"hypothesize":[66],"utilization":[68],"bovine-specific":[70,274],"factors":[73],"would":[74],"be":[75,386],"more":[76],"beneficial":[77],"observed":[80],"effects":[81],"factors.":[84],"\\n\\nTo":[85,267],"test":[86,333],"this":[87,246,336],"hypothesis,":[88],"wild":[89,127],"type,":[90],"BMP15":[92,129,297,312,329],"was":[93,123,136,174],"cloned":[94,137],"using":[95,227,249],"reverse":[96],"transcriptase":[97],"PCR":[98,309],"RNA":[100],"obtained":[101],"ovarian":[104],"tissue.":[105],"For":[106],"improved":[107],"detection":[108],"purification":[110,168],"biologically":[113],"active":[114],"recombinant":[115,295],"protein,":[116],"a":[117,214,251,331,343,382],"FLAG":[118,187],"tag":[119],"peptide":[120,188],"(Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys)":[122],"incorporated":[124],"into":[125,138],"type":[128],"gene":[130,350],"by":[131,176,330],"PCR.":[132],"This":[133],"modified":[134],"protein":[135,255],"pCDNA":[140],"3":[141],"expression":[143,351],"vector.":[144],"HEK-293":[145,203],"(human":[146],"embryonic":[147],"kidney":[148],"293)":[149],"FBK":[151,211],"(fetal":[152],"kidney)":[154],"cell":[155,205,304],"lines":[156],"were":[157,198,225,282],"transfected":[158,204],"via":[159],"electroporation":[160],"then":[162],"selected":[163],"homogeneity.":[165],"\\n\\nCollection":[166],"rbFL-BMP15":[170,299,347],"conditioned":[172],"medium":[173],"accomplished":[175],"incubation":[177],"with":[178,327,362,375],"anti-FLAG":[179],"affinity":[180],"gel":[181],"use":[184],"3X":[186],"elution.":[190],"Peptides":[191],"15.4":[193],"kDa":[194,197,217],"17":[196,216],"noted":[199],"human":[202,296,301,328],"line,":[206],"while":[207],"contrast,":[209],"cells":[212,233],"produced":[213],"single":[215],"protein.":[218],"Bioactivity":[219],"BMP":[221,239],"receptor":[222,256],"signaling":[223,240,247],"specificity":[224],"ascertained":[226],"treatment":[230,326],"HeLa":[232],"Western":[235],"blotting":[236],"molecule":[241],"phosphorylated-SMAD":[242],"1/5.":[243],"Inhibition":[244],"cascade":[248],"dorsomorphin,":[250],"selective":[252],"bone":[253],"morphogenic":[254],"I":[257],"inhibitor,":[258],"purified":[261],"proteins":[262],"served":[263],"as":[264],"BMP15-like":[265],"agonists.":[266],"examine":[268],"impact":[270],"our":[272],"purified,":[273],"peptides":[275],"on":[276,348],"maturation,":[278],"cumulus":[279,356],"complexes":[281,358],"matured":[285],"24":[287,360],"hours":[288,361],"presence":[291],"100":[293,363],"ng/ml":[294,364],"or":[298,302],"lines.":[305],"Real":[306],"time":[307,370],"quantitative":[308],"analysis":[310],"stimulated":[313],"genes,":[314],"PTGS2":[315],"TSG6,":[317],"revealed":[318],"statistically":[319],"significant":[320,344],"increases":[321],"transcript":[323],"level":[324],"Dunnett\u2019s":[332],"(p<0.05).":[334],"In":[335],"report,":[337],"however,":[338],"we":[339],"failed":[340],"detect":[342],"affect":[345],"mature":[355],"at":[359],"rbFL-BMP15.":[365],"Future":[366],"studies":[367],"including":[368],"differing":[369],"points":[371],"concentrations,":[373],"along":[374],"GDF9":[379],"form":[381],"possible":[383],"heterodimer":[384],"should":[385],"investigated":[387],"possibility":[390],"improving":[392],"maturation.":[397]},"counts_by_year":[],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2016-06-24T00:00:00"}