{"id":"https://openalex.org/W2286014395","doi":null,"title":"The physiological role of peroxiredoxin 2 in human erythrocytes: a kinetic analysis","display_name":"The physiological role of peroxiredoxin 2 in human erythrocytes: a kinetic analysis","publication_year":2011,"publication_date":"2011-01-01","ids":{"openalex":"https://openalex.org/W2286014395","mag":"2286014395"},"language":"en","primary_location":{"id":"pmh:oai:repositorio.ulisboa.pt:10451/8168","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10451/8168","pdf_url":null,"source":{"id":"https://openalex.org/S4306402433","display_name":"Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"master thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://hdl.handle.net/10451/8168","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5039489405","display_name":"Rui Benfeitas","orcid":"https://orcid.org/0000-0001-7972-0083","observed_orcids":["https://orcid.org/0000-0001-7972-0083"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Rui Benfeitas","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0001-7972-0083","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5039489405"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11884","display_name":"Redox biology and oxidative stress","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11884","display_name":"Redox biology and oxidative stress","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10445","display_name":"Antioxidant Activity and Oxidative Stress","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2704","display_name":"Biochemistry (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T13013","display_name":"Free Radicals and Antioxidants","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/peroxiredoxin-2","display_name":"peroxiredoxin 2","score":0.9480000138282776},{"id":"https://openalex.org/keywords/human-erythrocytes","display_name":"human erythrocytes","score":0.968999981880188},{"id":"https://openalex.org/keywords/antioxidant-enzymes","display_name":"antioxidant enzymes","score":0.6790000200271606},{"id":"https://openalex.org/keywords/catalase","display_name":"catalase","score":0.9589999914169312},{"id":"https://openalex.org/keywords/glutathione-peroxidase-1","display_name":"glutathione peroxidase 1","score":0.9950000047683716},{"id":"https://openalex.org/keywords/nadph-oxidation","display_name":"NADPH oxidation","score":0.7979999780654907},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.7070000171661377},{"id":"https://openalex.org/keywords/mathematical-modeling","display_name":"mathematical modeling","score":0.6800000071525574},{"id":"https://openalex.org/keywords/oxidative-stress","display_name":"oxidative stress","score":0.5830000042915344},{"id":"https://openalex.org/keywords/hydrogen-peroxide-scavenging","display_name":"hydrogen peroxide scavenging","score":0.6959999799728394},{"id":"https://openalex.org/keywords/enzyme-redundancy","display_name":"enzyme redundancy","score":0.49300000071525574},{"id":"https://openalex.org/keywords/membrane-permeation","display_name":"membrane permeation","score":0.5529999732971191},{"id":"https://openalex.org/keywords/redox-homeostasis","display_name":"redox homeostasis","score":0.5299999713897705}],"concepts":[{"id":"https://openalex.org/C135889238","wikidata":"https://www.wikidata.org/wiki/Q46276","display_name":"Kinetic energy","level":2,"score":0.5550308227539062},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3270992040634155},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.17579656839370728},{"id":"https://openalex.org/C74650414","wikidata":"https://www.wikidata.org/wiki/Q11397","display_name":"Classical mechanics","level":1,"score":0.09666246175765991}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:repositorio.ulisboa.pt:10451/8168","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10451/8168","pdf_url":null,"source":{"id":"https://openalex.org/S4306402433","display_name":"Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"master thesis"},{"id":"mag:2286014395","doi":null,"is_oa":false,"landing_page_url":"https://repositorio.ul.pt/bitstream/10451/8168/1/ulfc103614_tm_Rui_Benfeitas.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:repositorio.ulisboa.pt:10451/8168","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10451/8168","pdf_url":null,"source":{"id":"https://openalex.org/S4306402433","display_name":"Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"master thesis"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.6240000128746033,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":178,"referenced_works":["https://openalex.org/W11285659","https://openalex.org/W78159807","https://openalex.org/W148527043","https://openalex.org/W160949418","https://openalex.org/W174942032","https://openalex.org/W191793640","https://openalex.org/W291480391","https://openalex.org/W579754915","https://openalex.org/W1270671049","https://openalex.org/W1483721839","https://openalex.org/W1484180915","https://openalex.org/W1495200985","https://openalex.org/W1498464386","https://openalex.org/W1520661220","https://openalex.org/W1529662354","https://openalex.org/W1535086203","https://openalex.org/W1541649582","https://openalex.org/W1554607334","https://openalex.org/W1554849134","https://openalex.org/W1563384847","https://openalex.org/W1563709336","https://openalex.org/W1567108311","https://openalex.org/W1572688882","https://openalex.org/W1579364249","https://openalex.org/W1585735064","https://openalex.org/W1589043152","https://openalex.org/W1589413892","https://openalex.org/W1595015475","https://openalex.org/W1595716614","https://openalex.org/W1607500403","https://openalex.org/W1630599512","https://openalex.org/W1651185880","https://openalex.org/W1782880405","https://openalex.org/W1787410800","https://openalex.org/W1796522495","https://openalex.org/W1809723326","https://openalex.org/W1965525795","https://openalex.org/W1966041739","https://openalex.org/W1966162689","https://openalex.org/W1971679379","https://openalex.org/W1972875046","https://openalex.org/W1975655866","https://openalex.org/W1975984844","https://openalex.org/W1976842211","https://openalex.org/W1976913935","https://openalex.org/W1981419673","https://openalex.org/W1982583342","https://openalex.org/W1984400536","https://openalex.org/W1988175750","https://openalex.org/W1989407459","https://openalex.org/W1989486155","https://openalex.org/W1993055408","https://openalex.org/W1993058298","https://openalex.org/W1993509416","https://openalex.org/W1995000575","https://openalex.org/W1995731823","https://openalex.org/W1998214455","https://openalex.org/W1999382188","https://openalex.org/W1999704754","https://openalex.org/W2001923529","https://openalex.org/W2002449018","https://openalex.org/W2004110672","https://openalex.org/W2004595749","https://openalex.org/W2005766024","https://openalex.org/W2006313098","https://openalex.org/W2006817878","https://openalex.org/W2008228060","https://openalex.org/W2009938478","https://openalex.org/W2013356870","https://openalex.org/W2013557200","https://openalex.org/W2013947447","https://openalex.org/W2021872718","https://openalex.org/W2022239851","https://openalex.org/W2022312170","https://openalex.org/W2023111791","https://openalex.org/W2025636236","https://openalex.org/W2026365242","https://openalex.org/W2029130174","https://openalex.org/W2030596036","https://openalex.org/W2031002652","https://openalex.org/W2033897696","https://openalex.org/W2034260628","https://openalex.org/W2034430205","https://openalex.org/W2035800570","https://openalex.org/W2037163903","https://openalex.org/W2041489973","https://openalex.org/W2041583275","https://openalex.org/W2043065121","https://openalex.org/W2046483489","https://openalex.org/W2047110122","https://openalex.org/W2047995322","https://openalex.org/W2049608614","https://openalex.org/W2049713754","https://openalex.org/W2049960582","https://openalex.org/W2050795431","https://openalex.org/W2051648478","https://openalex.org/W2051687003","https://openalex.org/W2053077887","https://openalex.org/W2053985315","https://openalex.org/W2056135909","https://openalex.org/W2060771197","https://openalex.org/W2061698776","https://openalex.org/W2062219599","https://openalex.org/W2062901635","https://openalex.org/W2065351980","https://openalex.org/W2065891531","https://openalex.org/W2067305246","https://openalex.org/W2067632283","https://openalex.org/W2068131861","https://openalex.org/W2068146717","https://openalex.org/W2069463594","https://openalex.org/W2069518570","https://openalex.org/W2069578064","https://openalex.org/W2070309383","https://openalex.org/W2080163237","https://openalex.org/W2080632862","https://openalex.org/W2080678575","https://openalex.org/W2080770592","https://openalex.org/W2081153915","https://openalex.org/W2084781219","https://openalex.org/W2085312268","https://openalex.org/W2089772634","https://openalex.org/W2090383198","https://openalex.org/W2090884527","https://openalex.org/W2091091295","https://openalex.org/W2092463196","https://openalex.org/W2092570328","https://openalex.org/W2092597744","https://openalex.org/W2094341424","https://openalex.org/W2095635206","https://openalex.org/W2096471386","https://openalex.org/W2097155911","https://openalex.org/W2098048169","https://openalex.org/W2098651583","https://openalex.org/W2098688509","https://openalex.org/W2098795538","https://openalex.org/W2099409085","https://openalex.org/W2099675490","https://openalex.org/W2101166931","https://openalex.org/W2102934052","https://openalex.org/W2102944887","https://openalex.org/W2112645157","https://openalex.org/W2114467922","https://openalex.org/W2116012545","https://openalex.org/W2118940991","https://openalex.org/W2120353932","https://openalex.org/W2121221059","https://openalex.org/W2121926391","https://openalex.org/W2124013207","https://openalex.org/W2125542444","https://openalex.org/W2127750862","https://openalex.org/W2129997331","https://openalex.org/W2132826785","https://openalex.org/W2136772857","https://openalex.org/W2137511741","https://openalex.org/W2146989945","https://openalex.org/W2147228426","https://openalex.org/W2151454486","https://openalex.org/W2154393775","https://openalex.org/W2154761807","https://openalex.org/W2155376494","https://openalex.org/W2156861923","https://openalex.org/W2158496331","https://openalex.org/W2161901377","https://openalex.org/W2162494662","https://openalex.org/W2168721627","https://openalex.org/W2171913440","https://openalex.org/W2270677749","https://openalex.org/W2286498409","https://openalex.org/W2314203153","https://openalex.org/W2331302513","https://openalex.org/W2397988498","https://openalex.org/W2400444806","https://openalex.org/W2414168452","https://openalex.org/W2414326059","https://openalex.org/W2415357864","https://openalex.org/W2765517870","https://openalex.org/W3130674902"],"related_works":["https://openalex.org/W2921931161","https://openalex.org/W7097855163","https://openalex.org/W2315350508","https://openalex.org/W2327810509","https://openalex.org/W2078985977","https://openalex.org/W1999382188","https://openalex.org/W2104406973","https://openalex.org/W2035492487","https://openalex.org/W2970118216","https://openalex.org/W7207943568"],"abstract_inverted_index":{"Peroxiredoxin":[0],"2":[1],"(Prx2)":[2],"is":[3,29,49,57,75,101,258,265,283,452,497,516,547,576],"a":[4,30,186,354,385,445,535],"hydrogen":[5,24,41,78],"peroxide":[6,25],"reducing":[7,67,375],"protein":[8],"abundant":[9],"in":[10,135,227,328,350,389,448,467,493,498,510,524,538,560,595,644],"human":[11,81,136,155,351,581],"erythrocytes.":[12,352],"Due":[13],"to":[14,69,104,145,203,232,343,370,394,416,435,459,488,531,556,566,612],"its":[15,44,70,599],"large":[16],"concentration":[17,191,198,323,391,404],"(3.7\u00d710-4":[18],"M)":[19],"and":[20,142,163,242,248,309,324,366,405,475,639,649],"high":[21],"reactivity":[22],"with":[23,471],"(k~108":[26],"M-1s-1)":[27],"it":[28,74],"potentially":[31],"very":[32,548],"relevant":[33],"antioxidant":[34,53,90,637],"for":[35,46,66,132,246,285,311,379,396,507,564,601,623,633],"the":[36,60,121,151,168,172,180,214,217,222,251,276,289,320,348,376,382,397,417,455,461,472,494,499,502,521,527,577,609,624,634],"defense":[37,48,134,224,312,579,625],"of":[38,55,167,171,213,250,279,347,399,422,430,444,501,544,580,636],"erythrocytes":[39,64,82,110,156,228,264,272,302,364,518,582],"against":[40,157,225,313,583,626],"peroxide.":[42,79],"However,":[43,534],"contribution":[45,600],"H2O2":[47,87,99,122,133,189,196,226,252,322,403,492,584,602,615],"not":[50,116,304,607],"clear.":[51],"The":[52,165,441],"activity":[54,91,125,421],"Prx2":[56,68,130,173,202,207,237,257,282,291,390,439,480,515,539,565,575,590,648],"affected":[58],"by":[59,77,263,267,362,374,454],"limited":[61,266],"capacity":[62],"that":[63,234,301,428,574],"have":[65,630],"active":[71],"form":[72,523],"after":[73],"oxidized":[76],"Further,":[80],"also":[83,299,426],"possess":[84],"catalase,":[85,120],"an":[86,449],"scavenger":[88,123],"whose":[89,124],"entails":[92],"minimal":[93],"NADPH":[94,105],"consumption.":[95,603],"In":[96],"contrast,":[97],"Prx2-mediated":[98],"reduction":[100],"stoichiometrically":[102],"coupled":[103],"oxidation.":[106,440],"Why":[107,113],"do":[108,114],"then":[109,208,244],"need":[111],"Prx2?":[112],"they":[115,617],"rely":[117,305,620],"only":[118],"on":[119,307,402,621],"requires":[126],"less":[127,368],"NADPH?":[128],"Is":[129],"redundant":[131],"erythrocytes?":[137],"We":[138,149],"used":[139],"kinetic":[140],"analyses":[141,298,425],"mathematical":[143],"modeling":[144],"clarify":[146],"these":[147,464],"issues.":[148],"considered":[150],"main":[152,223,277,578],"processes":[153],"protecting":[154],"H2O2:":[158],"glutathione":[159],"peroxidase":[160],"(GPx1),":[161],"catalase":[162,220,308,333,400,622,650],"Prx2.":[164,423],"analysis":[166],"dynamic":[169],"behavior":[170],"system":[174],"under":[175,293,585],"various":[176],"oxidative":[177,183,295,410,528],"conditions":[178],"indicates":[179],"following.":[181],"Basal":[182],"conditions,":[184],"assuming":[185],"sustained":[187,230],"extracellular":[188],"(eH2O2)":[190],"<10-7":[192],"M":[193,200,491],"or":[194,373],"intracellular":[195],"(iH2O2)":[197],"<2\u00d710-11":[199],"allow":[201],"remain":[204],"fully":[205,235,259,316,568],"reduced.":[206,569],"consumes":[209],"more":[210],"than":[211],"99%":[212],"iH2O2.":[215],"On":[216,381],"other":[218,383,511],"hand,":[219,384],"becomes":[221],"facing":[229],"exposure":[231],"loads":[233,504],"oxidize":[236],"(eH2O2>":[238],"1.4\u00d710-6":[239],"M).":[240],"Catalase":[241],"GPx1":[243],"contribute":[245],"84%":[247],"16%":[249],"consumption,":[253],"respectively.":[254],"Even":[255],"when":[256],"oxidized,":[260,517],"eH2O2":[261],"consumption":[262],"membrane":[268],"permeation.":[269],"Thus,":[270],"although":[271],"are":[273,414,466,554,651],"quite":[274],"likely":[275],"sink":[278],"plasma":[280,614],"H2O2,":[281],"dispensable":[284],"this":[286,605],"role.":[287],"Despite":[288],"slow":[290],"regeneration":[292],"strong":[294],"loads,":[296],"our":[297,571],"suggest":[300,427],"could":[303,356],"solely":[306],"Gpx1":[310],"H2O2.":[314,627],"To":[315],"replace":[317],"Prx2,":[318],"maintaining":[319],"same":[321],"response":[325,406],"time":[326,407,443,456,562],"as":[327],"normal":[329],"erythrocytes,":[330],"~11\u00d710-12":[331],"g":[332,335],"(6\u00d710-12":[334],"GPx1)":[336],"would":[337,341,392],"be":[338,567,593],"required.":[339],"This":[340,550],"amount":[342],"32%":[344],"(18":[345],"%)":[346],"hemoglobin":[349],"Such":[353],"solution":[355],"therefore":[357],"impair":[358,608],"oxygen":[359,641],"transport":[360],"either":[361],"making":[363],"bigger,":[365],"thus":[367],"able":[369],"cross":[371,460],"capillaries,":[372,462],"space":[377],"available":[378],"hemoglobin.":[380],"<1":[386],"%":[387],"increase":[388,537],"suffice":[393],"compensate":[395],"effect":[398],"elimination":[401],"at":[408],"basal":[409,532],"loads.":[411],"These":[412],"differences":[413],"due":[415],"much":[418],"higher":[419],"specific":[420],"Our":[424,628],"crossing":[429,543],"inflammation":[431,450,485,545,596],"sites":[432,546],"may":[433,592],"lead":[434,487],"substantial":[436],"but":[437],"brief":[438],"residence":[442],"circulating":[446],"erythrocyte":[447],"site":[451],"determined":[453,506],"(~2":[457],"s)":[458],"where":[463,647],"cells":[465],"most":[468,477,586],"intimate":[469],"contact":[470],"vasculature":[473],"walls":[474],"circulate":[476],"slowly.":[478],"Full":[479],"oxidation":[481,540,591],"occurs":[482],"if":[483,526],"those":[484],"foci":[486,509],">":[489],"25\u00d710-6":[490],"plasma,":[495],"which":[496],"scale":[500],"strongest":[503],"experimentally":[505],"inflammatory":[508],"organisms.":[512],"Once":[513],"all":[514],"can":[519,618],"regenerate":[520],"reduced":[522],"minutes":[525],"load":[529],"returns":[530],"values.":[533],"stepwise":[536],"through":[541],"repeated":[542],"unlikely.":[549],"because":[551,616],"such":[552],"crossings":[553],"expected":[555],"occur":[557],"many":[558],"hours":[559],"between,":[561],"enough":[563],"Altogether,":[570],"results":[572,629],"show":[573],"physiological":[587],"circumstances.":[588],"Extensive":[589],"attained":[594],"sites,":[597],"affecting":[598],"Yet,":[604],"does":[606],"erythrocytes\u2019":[610],"ability":[611],"scavenge":[613],"still":[619],"important":[631],"implications":[632],"study":[635],"protection":[638],"reactive":[640],"species-associated":[642],"diseases":[643],"mammalian":[645],"cells,":[646],"found.":[652]},"counts_by_year":[{"year":2025,"cited_by_count":1}],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2016-06-24T00:00:00"}
