{"id":"https://openalex.org/W228586527","doi":null,"title":"A New Retinoid Signaling Molecule in the Retinal Pigment Epithelium: RPE45","display_name":"A New Retinoid Signaling Molecule in the Retinal Pigment Epithelium: RPE45","publication_year":2008,"publication_date":"2008-05-14","ids":{"openalex":"https://openalex.org/W228586527","mag":"228586527"},"language":"en","primary_location":{"id":"mag:228586527","doi":null,"is_oa":false,"landing_page_url":"https://iovs.arvojournals.org/article.aspx?articleid=2379914","pdf_url":null,"source":{"id":"https://openalex.org/S76304953","display_name":"Investigative Ophthalmology & Visual Science","issn_l":"0146-0404","issn":["0146-0404","1552-5783"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310321950","host_organization_name":"Cadmus Press","host_organization_lineage":["https://openalex.org/P4310321950"],"host_organization_lineage_names":["Cadmus Press"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Investigative Ophthalmology & Visual Science","raw_type":null},"volume":null,"type":"article","indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5069496183","display_name":"Wan Jin Jahng","orcid":"https://orcid.org/0000-0001-8241-7739","observed_orcids":["https://orcid.org/0000-0001-8241-7739"]},"institutions":[{"id":"https://openalex.org/I155781252","display_name":"University of South Carolina","ror":"https://ror.org/02b6qw903","country_code":"US","type":"education","lineage":["https://openalex.org/I155781252"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Wan Jin Jahng","raw_affiliation_strings":["University of South Carolina, Columbia, South Carolina"],"raw_orcid":"https://orcid.org/0000-0001-8241-7739","affiliations":[{"raw_affiliation_string":"University of South Carolina, Columbia, South Carolina","institution_ids":["https://openalex.org/I155781252"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005045781","display_name":"Hilal Arnouk","orcid":"https://orcid.org/0000-0002-8448-0798","observed_orcids":["https://orcid.org/0000-0002-8448-0798"]},"institutions":[{"id":"https://openalex.org/I155781252","display_name":"University of South Carolina","ror":"https://ror.org/02b6qw903","country_code":"US","type":"education","lineage":["https://openalex.org/I155781252"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Hilal Arnouk","raw_affiliation_strings":["University of South Carolina, Columbia, South Carolina"],"raw_orcid":"https://orcid.org/0000-0002-8448-0798","affiliations":[{"raw_affiliation_string":"University of South Carolina, Columbia, South Carolina","institution_ids":["https://openalex.org/I155781252"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060533240","display_name":"Ruiming Zhang","orcid":"https://orcid.org/0009-0007-5491-582X","observed_orcids":["https://orcid.org/0009-0007-5491-582X"]},"institutions":[{"id":"https://openalex.org/I155781252","display_name":"University of South Carolina","ror":"https://ror.org/02b6qw903","country_code":"US","type":"education","lineage":["https://openalex.org/I155781252"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R. Zhang","raw_affiliation_strings":["University of South Carolina, Columbia, South Carolina"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of South Carolina, Columbia, South Carolina","institution_ids":["https://openalex.org/I155781252"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063382050","display_name":"Folami Lamoke","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I155781252","display_name":"University of South Carolina","ror":"https://ror.org/02b6qw903","country_code":"US","type":"education","lineage":["https://openalex.org/I155781252"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Folami Lamoke","raw_affiliation_strings":["University of South Carolina, Columbia, South Carolina"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of South Carolina, Columbia, South Carolina","institution_ids":["https://openalex.org/I155781252"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008551171","display_name":"Richard C. Hunt","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I155781252","display_name":"University of South Carolina","ror":"https://ror.org/02b6qw903","country_code":"US","type":"education","lineage":["https://openalex.org/I155781252"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Richard C. Hunt","raw_affiliation_strings":["Univ of South Carolina, Columbia, South Carolina"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Univ of South Carolina, Columbia, South Carolina","institution_ids":["https://openalex.org/I155781252"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074274548","display_name":"David M. Hunt","orcid":"https://orcid.org/0000-0002-9264-3948","observed_orcids":["https://orcid.org/0000-0002-9264-3948"]},"institutions":[{"id":"https://openalex.org/I155781252","display_name":"University of South Carolina","ror":"https://ror.org/02b6qw903","country_code":"US","type":"education","lineage":["https://openalex.org/I155781252"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"David M. Hunt","raw_affiliation_strings":["Univ of South Carolina, Columbia, South Carolina"],"raw_orcid":"https://orcid.org/0000-0002-9264-3948","affiliations":[{"raw_affiliation_string":"Univ of South Carolina, Columbia, South Carolina","institution_ids":["https://openalex.org/I155781252"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057956774","display_name":"William J.M. Hrushesky","orcid":"https://orcid.org/0000-0002-0955-3192","observed_orcids":["https://orcid.org/0000-0002-0955-3192"]},"institutions":[{"id":"https://openalex.org/I155781252","display_name":"University of South Carolina","ror":"https://ror.org/02b6qw903","country_code":"US","type":"education","lineage":["https://openalex.org/I155781252"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"William J.M. Hrushesky","raw_affiliation_strings":["Univ of South Carolina, Columbia, South Carolina"],"raw_orcid":"https://orcid.org/0000-0002-0955-3192","affiliations":[{"raw_affiliation_string":"Univ of South Carolina, Columbia, South Carolina","institution_ids":["https://openalex.org/I155781252"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108588689","display_name":"Patricia Ann Wood","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I155781252","display_name":"University of South Carolina","ror":"https://ror.org/02b6qw903","country_code":"US","type":"education","lineage":["https://openalex.org/I155781252"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Patricia A. Wood","raw_affiliation_strings":["Univ of South Carolina, Columbia, South Carolina"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Univ of South Carolina, Columbia, South Carolina","institution_ids":["https://openalex.org/I155781252"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018012990","display_name":"Robert R. Rando","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Robert R. Rando","raw_affiliation_strings":["Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts","institution_ids":["https://openalex.org/I136199984"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5066185175","display_name":"H. Lee","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I155781252","display_name":"University of South Carolina","ror":"https://ror.org/02b6qw903","country_code":"US","type":"education","lineage":["https://openalex.org/I155781252"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"H. Lee","raw_affiliation_strings":["University of South Carolina, Columbia, South Carolina"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of South Carolina, Columbia, South Carolina","institution_ids":["https://openalex.org/I155781252"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":1850,"currency":"USD","value_usd":1850},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.01933259,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"49","issue":"13","first_page":"4531","last_page":"4531"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10691","display_name":"Retinal Development and Disorders","score":0.8148999810218811,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10691","display_name":"Retinal Development and Disorders","score":0.8148999810218811,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10170","display_name":"Retinal Diseases and Treatments","score":0.15970000624656677,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11559","display_name":"Retinoids in leukemia and cellular processes","score":0.008299999870359898,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/rpe65","display_name":"RPE65","score":0.9990000128746033},{"id":"https://openalex.org/keywords/retinal-pigment-epithelium","display_name":"retinal pigment epithelium","score":0.9739999771118164},{"id":"https://openalex.org/keywords/retinoid-signaling","display_name":"retinoid signaling","score":0.8109999895095825},{"id":"https://openalex.org/keywords/visual-cycle","display_name":"visual cycle","score":0.9490000009536743},{"id":"https://openalex.org/keywords/light-exposure","display_name":"light exposure","score":0.5929999947547913},{"id":"https://openalex.org/keywords/oxidative-stress","display_name":"oxidative stress","score":0.9819999933242798},{"id":"https://openalex.org/keywords/reactive-oxygen-species","display_name":"reactive oxygen species","score":0.9789999723434448},{"id":"https://openalex.org/keywords/caspase-cleavage","display_name":"caspase cleavage","score":0.8849999904632568},{"id":"https://openalex.org/keywords/ubiquitination","display_name":"ubiquitination","score":0.9850000143051147},{"id":"https://openalex.org/keywords/post-transcriptional-regulation","display_name":"post-transcriptional regulation","score":0.8939999938011169}],"concepts":[{"id":"https://openalex.org/C2780724529","wikidata":"https://www.wikidata.org/wiki/Q18031223","display_name":"RPE65","level":4,"score":0.9590776562690735},{"id":"https://openalex.org/C2778024200","wikidata":"https://www.wikidata.org/wiki/Q2346242","display_name":"Retinal pigment epithelium","level":3,"score":0.6486839056015015},{"id":"https://openalex.org/C2776415932","wikidata":"https://www.wikidata.org/wiki/Q48743","display_name":"Western blot","level":3,"score":0.5733546018600464},{"id":"https://openalex.org/C46111723","wikidata":"https://www.wikidata.org/wiki/Q471857","display_name":"Proteomics","level":3,"score":0.49531498551368713},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.49527183175086975},{"id":"https://openalex.org/C2776151105","wikidata":"https://www.wikidata.org/wiki/Q898814","display_name":"Oxidative stress","level":2,"score":0.48948729038238525},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4760686755180359},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4637070298194885},{"id":"https://openalex.org/C2910190745","wikidata":"https://www.wikidata.org/wiki/Q5122112","display_name":"Cis-trans-Isomerases","level":5,"score":0.45682859420776367},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.39254990220069885},{"id":"https://openalex.org/C2780827179","wikidata":"https://www.wikidata.org/wiki/Q422001","display_name":"Retinal","level":2,"score":0.3855621814727783},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.34994634985923767},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.06375443935394287},{"id":"https://openalex.org/C2781264208","wikidata":"https://www.wikidata.org/wiki/Q118026","display_name":"Isomerase","level":3,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C2910677434","wikidata":"https://www.wikidata.org/wiki/Q426411","display_name":"Peptidylprolyl isomerase","level":4,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:228586527","doi":null,"is_oa":false,"landing_page_url":"https://iovs.arvojournals.org/article.aspx?articleid=2379914","pdf_url":null,"source":{"id":"https://openalex.org/S76304953","display_name":"Investigative Ophthalmology & Visual Science","issn_l":"0146-0404","issn":["0146-0404","1552-5783"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310321950","host_organization_name":"Cadmus Press","host_organization_lineage":["https://openalex.org/P4310321950"],"host_organization_lineage_names":["Cadmus Press"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Investigative Ophthalmology & Visual Science","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.504800021648407,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2025000308","https://openalex.org/W2051943009","https://openalex.org/W1991596247","https://openalex.org/W2014978982","https://openalex.org/W2375559938","https://openalex.org/W2046258960","https://openalex.org/W43468052","https://openalex.org/W2981898784","https://openalex.org/W748990171","https://openalex.org/W1509311142"],"abstract_inverted_index":{"Purpose:The":[0],"control":[1],"elements":[2],"that":[3,114,146,166,194,212,269],"regulate":[4],"the":[5,45,48,167,199,213,235,256,261,276],"overall":[6],"biosynthetic":[7],"pathway":[8],"to":[9,22,43,106,222,271],"11-cis-retinal":[10],"under":[11],"light":[12,49,129,161,204,220,236,253,272],"and":[13,37,50,56,80,94,131,162,189,205,224,252,273],"oxidative":[14,51,132,240,250],"stress":[15,52,251],"are":[16,174],"not":[17],"well":[18],"understood.":[19],"In":[20],"order":[21],"study":[23,44,107],"this":[24,181],"important":[25],"isomerization/hydrolysis":[26],"step,":[27],"we":[28],"used":[29],"a":[30,58,115,122,141,177,226,247,266],"differential":[31,168],"proteomics":[32,184],"approach":[33],"with":[34,155],"bovine,":[35],"murine":[36],"human":[38],"retinal":[39],"pigment":[40],"epithelial":[41],"cells":[42],"effects":[46],"of":[47,62,110,125,152,170,198,215,229,263],"on":[53],"RPE65":[54,156,172,195,216,264],"expression":[55,65,173],"RPE45,":[57,121,225],"caspase-mediated":[59],"cleavage":[60],"form":[61],"RPE65.":[63],"Methods:RPE65":[64],"levels":[66,169],"were":[67,104],"tested":[68],"in":[69,72,81,207,219,234,238,255,275],"various":[70],"conditions":[71],"vitro":[73],"(RPE":[74],"D407),":[75],"ex":[76],"vivo":[77,82],"(bovine,":[78],"mouse),":[79],"(mouse).":[83],"Proteomics":[84],"tools":[85],"such":[86],"as":[87,120,246,265],"2D":[88,186],"SDS-PAGE,":[89],"MALDITOF-TOF":[90],"mass":[91,190],"spectrometry":[92,191],"analysis":[93,192],"biochemical":[95],"methods":[96],"including":[97],"Western":[98],"blot,":[99],"immunohistochemistry,":[100],"retinoid":[101],"binding":[102],"assay":[103],"employed":[105],"functional":[108],"role":[109,262],"RPE45.":[111],"Results:We":[112],"found":[113],"new":[116],"45kDa":[117,227],"protein,":[118],"designated":[119],"truncated":[123],"fragment":[124,228],"RPE65,":[126,230],"appears":[127],"upon":[128],"exposure":[130,254],"stress.":[133,241],"RPE45":[134,147,243],"is":[135,196,217,231],"formed":[136],"by":[137,160,203],"recombinant":[138],"caspases":[139],"using":[140,185],"ubiquitination":[142,158],"mechanism.":[143],"We":[144,210],"show":[145],"results":[148],"from":[149],"an":[150],"interaction":[151],"specific":[153],"proteases":[154],"through":[157],"induced":[159],"ROS.":[163],"RT-PCR":[164],"revealed":[165],"stress-induced":[171],"regulated":[175,202],"at":[176],"posttranscriptional":[178],"level.":[179],"Conclusions:In":[180],"study,":[182],"comparative":[183],"gel":[187],"electrophoresis":[188],"reveal":[193],"one":[197],"major":[200],"proteins":[201],"ROS":[206,274],"RPE":[208],"cells.":[209],"find":[211],"level":[214],"increased":[218],"compared":[221],"dark,":[223],"present":[232],"only":[233],"or":[237],"high":[239],"Therefore,":[242],"can":[244],"serve":[245],"biomarker":[248],"for":[249],"eye.":[257],"These":[258],"findings":[259],"demonstrate":[260],"key":[267],"regulator":[268],"responds":[270],"visual":[277],"cycle.":[278]},"counts_by_year":[],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2016-06-24T00:00:00"}