{"id":"https://openalex.org/W2280145941","doi":"https://doi.org/10.1096/fasebj.24.1_supplement.681.11","title":"A Robust and Convenient Fluorimetric Assay for Measuring Acetylcholinesterase Activity and Screening Acetylcholinesterase Inhibitors","display_name":"A Robust and Convenient Fluorimetric Assay for Measuring Acetylcholinesterase Activity and Screening Acetylcholinesterase Inhibitors","publication_year":2010,"publication_date":"2010-04-01","ids":{"openalex":"https://openalex.org/W2280145941","doi":"https://doi.org/10.1096/fasebj.24.1_supplement.681.11","mag":"2280145941"},"language":"en","primary_location":{"id":"doi:10.1096/fasebj.24.1_supplement.681.11","doi":"https://doi.org/10.1096/fasebj.24.1_supplement.681.11","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.24.1_supplement.681.11","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5057976112","display_name":"Jinfang Liao","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jinfang Liao","raw_affiliation_strings":["ABD Bioquest, Inc., Sunnyvale, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ABD Bioquest, Inc., Sunnyvale, CA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5047161130","display_name":"Qin Zhao","orcid":"https://orcid.org/0000-0002-7412-3957","observed_orcids":["https://orcid.org/0000-0002-7412-3957"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Qin Zhao","raw_affiliation_strings":["ABD Bioquest, Inc., Sunnyvale, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ABD Bioquest, Inc., Sunnyvale, CA","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5110619228","display_name":"Zhenjun J. Diwu","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Zhenjun Diwu","raw_affiliation_strings":["ABD Bioquest, Inc., Sunnyvale, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ABD Bioquest, Inc., Sunnyvale, CA","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3740,"currency":"USD","value_usd":3740},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.22843474,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"24","issue":"S1","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12023","display_name":"Cholinesterase and Neurodegenerative Diseases","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T12023","display_name":"Cholinesterase and Neurodegenerative Diseases","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12412","display_name":"Enzyme function and inhibition","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10532","display_name":"Molecular Sensors and Ion Detection","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/acetylcholinesterase-activity","display_name":"acetylcholinesterase activity","score":0.8920000195503235},{"id":"https://openalex.org/keywords/acetylcholinesterase-inhibitors","display_name":"acetylcholinesterase inhibitors","score":0.9760000109672546},{"id":"https://openalex.org/keywords/fluorogenic-assay","display_name":"fluorogenic assay","score":0.734000027179718},{"id":"https://openalex.org/keywords/acetylthiocholine-hydrolysis","display_name":"acetylthiocholine hydrolysis","score":0.9890000224113464},{"id":"https://openalex.org/keywords/enzyme-activity-assay","display_name":"enzyme activity assay","score":0.7319999933242798},{"id":"https://openalex.org/keywords/alzheimer-s-disease","display_name":"Alzheimer's disease","score":0.6800000071525574},{"id":"https://openalex.org/keywords/myasthenia-gravis","display_name":"myasthenia gravis","score":0.996999979019165}],"concepts":[{"id":"https://openalex.org/C2778816929","wikidata":"https://www.wikidata.org/wiki/Q22676679","display_name":"Acetylcholinesterase","level":3,"score":0.9495631456375122},{"id":"https://openalex.org/C2909509320","wikidata":"https://www.wikidata.org/wiki/Q27458037","display_name":"Acetylthiocholine","level":5,"score":0.860405445098877},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7722575068473816},{"id":"https://openalex.org/C2778861558","wikidata":"https://www.wikidata.org/wiki/Q3101245","display_name":"Plate reader","level":3,"score":0.6303976774215698},{"id":"https://openalex.org/C2779059548","wikidata":"https://www.wikidata.org/wiki/Q383701","display_name":"Ach\u00e9","level":4,"score":0.6147922277450562},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.5043905973434448},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.48624861240386963},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4156104624271393},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.3981859087944031},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1096/fasebj.24.1_supplement.681.11","doi":"https://doi.org/10.1096/fasebj.24.1_supplement.681.11","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.24.1_supplement.681.11","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.428600013256073}],"sustainable_development_goals_aurora":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.6700000166893005}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1966529826","https://openalex.org/W2026058961","https://openalex.org/W2742701592","https://openalex.org/W2743329990","https://openalex.org/W906050321","https://openalex.org/W4389341197","https://openalex.org/W2048608944","https://openalex.org/W4410844262","https://openalex.org/W2004905110","https://openalex.org/W2073828109"],"abstract_inverted_index":{"Acetylcholinesterase":[0],"(AchE)":[1],"is":[2,31,74,88,95,131,160],"one":[3],"of":[4,23,101,122,127,136],"the":[5,18,37,83,98,119,134,139,166],"most":[6],"crucial":[7],"enzymes":[8],"for":[9,21,40,62,78],"nerve":[10],"responses":[11],"and":[12,26,50,66,147],"neurological":[13],"functions.":[14],"AChE":[15,42,80,140],"inhibitors":[16,43],"are":[17,44],"key":[19],"drugs":[20],"management":[22],"Alzheimer's":[24],"disease":[25],"myasthenia":[27],"gravis.":[28],"Although":[29],"AchE":[30,64,68],"a":[32,45,56,145,177],"proven":[33],"drug":[34],"development":[35],"target,":[36],"existing":[38,84],"technologies":[39],"screening":[41,67],"compromise":[46],"between":[47],"throughput,":[48],"sensitivity":[49],"physiological":[51],"relevance.":[52],"We":[53],"have":[54],"developed":[55],"robust":[57],"fluorimetric":[58,72,149],"Thiolite":[59,102,128],"Green\u2013based":[60],"assay":[61,94,150],"measuring":[63],"activities":[65],"inhibitors.":[69],"This":[70,106],"new":[71],"method":[73],"much":[75],"more":[76,163],"sensitive":[77,164],"detecting":[79],"activity":[81],"than":[82,165],"colorimetric":[85,168],"methods":[86],"that":[87],"generally":[89],"based":[90,96],"on":[91,97],"DNTB.":[92],"The":[93,124,142],"fluorogenic":[99,108],"reaction":[100,109],"Green":[103],"with":[104,181],"thiocholine.":[105],"specific":[107],"can":[110,172],"be":[111,173],"conveniently":[112],"used":[113],"to":[114,133,151],"quantify":[115],"thiocholine":[116],"produced":[117],"from":[118],"AChE\u2013induced":[120],"hydrolysis":[121],"acetylthiocholine.":[123],"fluorescence":[125,178],"intensity":[126],"Green\u2010thiocholine":[129],"adduct":[130],"proportional":[132],"formation":[135],"thiocholine,":[137],"therefore":[138],"activity.":[141],"Assay":[143],"provides":[144],"continuous":[146],"ultrasensitive":[148],"detect":[152],"as":[153,155],"little":[154],"0.1":[156],"mU/mL":[157],"AChE,":[158],"which":[159],"100":[161],"times":[162],"DNTB":[167],"method.":[169],"Its":[170],"signal":[171],"easily":[174],"read":[175],"by":[176],"microplate":[179],"reader":[180],"Ex/Em":[182],"=":[183],"490/520":[184],"nm.":[185]},"counts_by_year":[],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2016-06-24T00:00:00"}
