{"id":"https://openalex.org/W2278550528","doi":null,"title":"Evaluation of alternative reporter genes for the yeast two-hybrid system.","display_name":"Evaluation of alternative reporter genes for the yeast two-hybrid system.","publication_year":2003,"publication_date":"2003-03-31","ids":{"openalex":"https://openalex.org/W2278550528","mag":"2278550528","pmid":"https://pubmed.ncbi.nlm.nih.gov/12917809"},"language":"en","primary_location":{"id":"pmid:12917809","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12917809","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Genetics and molecular research : GMR","raw_type":"Evaluation Study"},"volume":null,"type":"article","indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5055175807","display_name":"Starling Al","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I110200422","display_name":"Universidade Federal de Minas Gerais","ror":"https://ror.org/0176yjw32","country_code":"BR","type":"education","lineage":["https://openalex.org/I110200422"]}],"countries":["BR"],"is_corresponding":false,"raw_author_name":"Alessandra L Starling","raw_affiliation_strings":["Departamento de Biologia Geral, Instituto de Ci\u00eancias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil","Departamento de Biologia Geral, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte MG, Brasil"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departamento de Biologia Geral, Instituto de Ci\u00eancias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil","institution_ids":["https://openalex.org/I110200422"]},{"raw_affiliation_string":"Departamento de Biologia Geral, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte MG, Brasil","institution_ids":["https://openalex.org/I110200422"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5059977137","display_name":"Jos\u00e9 Miguel Ortega","orcid":"https://orcid.org/0000-0002-8047-9191","observed_orcids":["https://orcid.org/0000-0002-8047-9191","https://orcid.org/0009-0007-2801-5561"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J Miguel Ortega","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017399345","display_name":"Kenneth J. Gollob","orcid":"https://orcid.org/0000-0003-4184-3867","observed_orcids":["https://orcid.org/0000-0003-4184-3867"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Kenneth J Gollob","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110314409","display_name":"Vicente Ej","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Elisabete J Vicente","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051865731","display_name":"Gisele Maria de Andrade de N\u00f3brega","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Gisele M Andrade-N\u00f3brega","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":null,"display_name":"M\u00f4nica B Rodriguez","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M\u00f4nica B Rodriguez","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.1149,"has_fulltext":false,"cited_by_count":13,"citation_normalized_percentile":{"value":0.46377536,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"2","issue":"1","first_page":"124","last_page":"35"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.9961000084877014,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.9961000084877014,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/yeast-two-hybrid","display_name":"yeast two-hybrid","score":0.9950000047683716},{"id":"https://openalex.org/keywords/reporter-genes","display_name":"reporter genes","score":0.9769999980926514},{"id":"https://openalex.org/keywords/protein-protein-interaction","display_name":"protein-protein interaction","score":0.953000009059906},{"id":"https://openalex.org/keywords/aequorea-victoria","display_name":"Aequorea victoria","score":0.6489999890327454},{"id":"https://openalex.org/keywords/enhanced-green-fluorescent-protein","display_name":"enhanced green fluorescent protein","score":0.9520000219345093},{"id":"https://openalex.org/keywords/flow-cytometry-screening","display_name":"flow cytometry screening","score":0.765999972820282}],"concepts":[{"id":"https://openalex.org/C161733203","wikidata":"https://www.wikidata.org/wiki/Q911828","display_name":"Reporter gene","level":4,"score":0.7458975911140442},{"id":"https://openalex.org/C142613039","wikidata":"https://www.wikidata.org/wiki/Q272983","display_name":"Green fluorescent protein","level":3,"score":0.730316698551178},{"id":"https://openalex.org/C2909003921","wikidata":"https://www.wikidata.org/wiki/Q6763786","display_name":"Aspergillus awamori","level":3,"score":0.6594604253768921},{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.6456766724586487},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.6002233624458313},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.510632336139679},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5062812566757202},{"id":"https://openalex.org/C174851938","wikidata":"https://www.wikidata.org/wiki/Q1337844","display_name":"Two-hybrid screening","level":3,"score":0.4781678318977356},{"id":"https://openalex.org/C127716648","wikidata":"https://www.wikidata.org/wiki/Q104053","display_name":"Phenotype","level":3,"score":0.44338247179985046},{"id":"https://openalex.org/C101762097","wikidata":"https://www.wikidata.org/wiki/Q224093","display_name":"Promoter","level":4,"score":0.4378451108932495},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4294233024120331},{"id":"https://openalex.org/C91779695","wikidata":"https://www.wikidata.org/wiki/Q3780824","display_name":"Coding region","level":3,"score":0.41193467378616333},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.32368505001068115},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.320384681224823},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.2854478359222412},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.26264122128486633},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.25565385818481445}],"mesh":[{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005087","descriptor_name":"Glucan 1,4-alpha-Glucosidase","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D005087","descriptor_name":"Glucan 1,4-alpha-Glucosidase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005434","descriptor_name":"Flow Cytometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008164","descriptor_name":"Luminescent Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D008164","descriptor_name":"Luminescent Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015003","descriptor_name":"Yeasts","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D016415","descriptor_name":"Sequence Alignment","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017930","descriptor_name":"Genes, Reporter","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D020798","descriptor_name":"Two-Hybrid System Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D049452","descriptor_name":"Green Fluorescent Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:12917809","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12917809","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Genetics and molecular research : GMR","raw_type":"Evaluation Study"},{"id":"mag:2278550528","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12917809","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2063111442","https://openalex.org/W2009452287","https://openalex.org/W2115124763","https://openalex.org/W2080764609","https://openalex.org/W68826470","https://openalex.org/W64221680","https://openalex.org/W1499960698","https://openalex.org/W2001960791","https://openalex.org/W2413619980","https://openalex.org/W2418001458"],"abstract_inverted_index":{"The":[0],"yeast":[1,30],"two-hybrid":[2,31],"system":[3,111],"is":[4],"a":[5],"powerful":[6],"tool":[7],"for":[8,17,28,77,100,123],"screening":[9,42],"protein-protein":[10],"interactions":[11],"and":[12,62],"has":[13],"also":[14],"been":[15],"used":[16],"large-scale":[18],"studies.":[19],"We":[20],"evaluated":[21],"two":[22],"protein-coding":[23],"sequences":[24],"as":[25,39],"reporter":[26,109],"genes":[27],"the":[29,107,115,126],"system,":[32],"to":[33,96],"determine":[34,97],"if":[35],"it":[36],"was":[37,75,94,112],"suitable":[38],"an":[40],"alternative":[41],"strategy.":[43],"Aspergillus":[44],"awamori":[45],"glucoamylase":[46],"activity":[47],"results":[48],"in":[49],"clear":[50],"haloes":[51],"around":[52],"colonies":[53],"producing":[54],"this":[55,78],"enzyme":[56],"after":[57],"growth":[58],"on":[59,72],"starch":[60],"plates":[61],"staining":[63],"with":[64,103,125],"iodine":[65],"vapors.":[66],"However,":[67],"transcription":[68],"activation":[69,119],"by":[70],"Gal4":[71,117],"Gal-regulated":[73],"promoters":[74],"insufficient":[76],"type":[79],"of":[80,86],"phenotypic":[81],"visualization.":[82],"A":[83],"modified":[84],"gene":[85,110],"Aequoria":[87],"victoria":[88],"enhanced":[89],"green":[90],"fluorescent":[91],"protein":[92],"(EGFP)":[93],"tested":[95],"its":[98],"suitability":[99],"interaction":[101],"screenings":[102],"flow":[104,127],"cytometry.":[105],"When":[106],"EGFP":[108],"incorporated":[113],"into":[114],"cells,":[116],"transcriptional":[118],"produced":[120],"sufficient":[121],"fluorescence":[122],"detection":[124],"cytometer,":[128],"especially":[129],"when":[130],"there":[131],"were":[132],"strong":[133],"interactions.":[134]},"counts_by_year":[{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
