{"id":"https://openalex.org/W2270601028","doi":"https://doi.org/10.1007/s12010-015-1961-z","title":"The Stability Enhancement of Nitrile Hydratase from Bordetella petrii by Swapping the C-terminal Domain of \u03b2 subunit","display_name":"The Stability Enhancement of Nitrile Hydratase from Bordetella petrii by Swapping the C-terminal Domain of \u03b2 subunit","publication_year":2015,"publication_date":"2015-12-21","ids":{"openalex":"https://openalex.org/W2270601028","doi":"https://doi.org/10.1007/s12010-015-1961-z","mag":"2270601028","pmid":"https://pubmed.ncbi.nlm.nih.gov/26686500"},"language":"en","primary_location":{"id":"doi:10.1007/s12010-015-1961-z","doi":"https://doi.org/10.1007/s12010-015-1961-z","is_oa":false,"landing_page_url":"https://doi.org/10.1007/s12010-015-1961-z","pdf_url":null,"source":{"id":"https://openalex.org/S153285774","display_name":"Applied Biochemistry and Biotechnology","issn_l":"0273-2289","issn":["0273-2289","1559-0291"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied Biochemistry and Biotechnology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101625160","display_name":"Weifeng Sun","orcid":"https://orcid.org/0000-0002-6571-7243","observed_orcids":["https://orcid.org/0000-0002-6571-7243"]},"institutions":[{"id":"https://openalex.org/I102345215","display_name":"Xihua University","ror":"https://ror.org/04gwtvf26","country_code":"CN","type":"education","lineage":["https://openalex.org/I102345215"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Weifeng Sun","raw_affiliation_strings":["Key Laboratory of Food and Biotechnology, School of Food and Biotechnology, Xihua University, Chengdu, Sichuan, 610039, People's Republic of China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Food and Biotechnology, School of Food and Biotechnology, Xihua University, Chengdu, Sichuan, 610039, People's Republic of China","institution_ids":["https://openalex.org/I102345215"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101043413","display_name":"Longbao Zhu","orcid":"https://orcid.org/0000-0003-2259-9387","observed_orcids":["https://orcid.org/0000-0003-2259-9387"]},"institutions":[{"id":"https://openalex.org/I70908550","display_name":"Anhui Polytechnic University","ror":"https://ror.org/041sj0284","country_code":"CN","type":"education","lineage":["https://openalex.org/I70908550"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Longbao Zhu","raw_affiliation_strings":["School of Biochemical Engineering, Anhui Polytechnic University, Anhui, 241000, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Biochemical Engineering, Anhui Polytechnic University, Anhui, 241000, China","institution_ids":["https://openalex.org/I70908550"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5022047992","display_name":"Xianggui Chen","orcid":"https://orcid.org/0000-0002-4167-8825","observed_orcids":["https://orcid.org/0000-0002-4167-8825"]},"institutions":[{"id":"https://openalex.org/I102345215","display_name":"Xihua University","ror":"https://ror.org/04gwtvf26","country_code":"CN","type":"education","lineage":["https://openalex.org/I102345215"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xianggui Chen","raw_affiliation_strings":["Key Laboratory of Food and Biotechnology, School of Food and Biotechnology, Xihua University, Chengdu, Sichuan, 610039, People's Republic of China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Food and Biotechnology, School of Food and Biotechnology, Xihua University, Chengdu, Sichuan, 610039, People's Republic of China","institution_ids":["https://openalex.org/I102345215"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089916822","display_name":"Lunjie Wu","orcid":"https://orcid.org/0000-0001-7802-6372","observed_orcids":["https://orcid.org/0000-0001-7802-6372"]},"institutions":[{"id":"https://openalex.org/I102345215","display_name":"Xihua University","ror":"https://ror.org/04gwtvf26","country_code":"CN","type":"education","lineage":["https://openalex.org/I102345215"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Lunjie Wu","raw_affiliation_strings":["Key Laboratory of Food and Biotechnology, School of Food and Biotechnology, Xihua University, Chengdu, Sichuan, 610039, People's Republic of China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Food and Biotechnology, School of Food and Biotechnology, Xihua University, Chengdu, Sichuan, 610039, People's Republic of China","institution_ids":["https://openalex.org/I102345215"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5067135999","display_name":"Zhemin Zhou","orcid":"https://orcid.org/0000-0003-0604-1885","observed_orcids":["https://orcid.org/0000-0003-0604-1885"]},"institutions":[{"id":"https://openalex.org/I111599522","display_name":"Jiangnan University","ror":"https://ror.org/04mkzax54","country_code":"CN","type":"education","lineage":["https://openalex.org/I111599522"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Zhemin Zhou","raw_affiliation_strings":["Key Laboratory of Industrial Biotechnology, School of Biotechnology, Jiangnan University, Wuxi, 214122, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Industrial Biotechnology, School of Biotechnology, Jiangnan University, Wuxi, 214122, China","institution_ids":["https://openalex.org/I111599522"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5103216660","display_name":"Yi Liu","orcid":"https://orcid.org/0000-0003-3110-3654","observed_orcids":["https://orcid.org/0000-0003-3110-3654"]},"institutions":[{"id":"https://openalex.org/I102345215","display_name":"Xihua University","ror":"https://ror.org/04gwtvf26","country_code":"CN","type":"education","lineage":["https://openalex.org/I102345215"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Yi Liu","raw_affiliation_strings":["Key Laboratory of Food and Biotechnology, School of Food and Biotechnology, Xihua University, Chengdu, Sichuan, 610039, People's Republic of China. puter_scholar@163.com","Key Laboratory of Food and Biotechnology, School of Food and Biotechnology, Xihua University, Chengdu, Sichuan, 610039, People's Republic of China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Food and Biotechnology, School of Food and Biotechnology, Xihua University, Chengdu, Sichuan, 610039, People's Republic of China. puter_scholar@163.com","institution_ids":["https://openalex.org/I102345215"]},{"raw_affiliation_string":"Key Laboratory of Food and Biotechnology, School of Food and Biotechnology, Xihua University, Chengdu, Sichuan, 610039, People's Republic of China","institution_ids":["https://openalex.org/I102345215"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5103216660"],"corresponding_institution_ids":["https://openalex.org/I102345215"],"apc_list":{"value":3890,"currency":"USD","value_usd":3890},"apc_paid":null,"fwci":0.1059,"has_fulltext":false,"cited_by_count":10,"citation_normalized_percentile":{"value":0.38636211,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"178","issue":"8","first_page":"1481","last_page":"1487"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.7577999830245972,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.7577999830245972,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.23569999635219574,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.0013000000035390258,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/nitrile-hydratase","display_name":"nitrile hydratase","score":1.0},{"id":"https://openalex.org/keywords/bordetella-petrii","display_name":"Bordetella petrii","score":0.9779999852180481},{"id":"https://openalex.org/keywords/pseudonocardia-thermophila","display_name":"Pseudonocardia thermophila","score":0.9829999804496765},{"id":"https://openalex.org/keywords/thermal-stability","display_name":"thermal stability","score":0.7360000014305115},{"id":"https://openalex.org/keywords/c-terminal-domain","display_name":"C-terminal domain","score":0.8769999742507935},{"id":"https://openalex.org/keywords/beta-subunit","display_name":"beta subunit","score":0.8019999861717224},{"id":"https://openalex.org/keywords/chimeric-enzymes","display_name":"chimeric enzymes","score":0.875},{"id":"https://openalex.org/keywords/domain-swapping","display_name":"domain swapping","score":0.7289999723434448},{"id":"https://openalex.org/keywords/melting-point","display_name":"melting point","score":0.4690000116825104}],"concepts":[{"id":"https://openalex.org/C2779122190","wikidata":"https://www.wikidata.org/wiki/Q7041403","display_name":"Nitrile hydratase","level":3,"score":0.9649113416671753},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.667911946773529},{"id":"https://openalex.org/C59061564","wikidata":"https://www.wikidata.org/wiki/Q7783071","display_name":"Thermal stability","level":2,"score":0.6430799961090088},{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.5414957404136658},{"id":"https://openalex.org/C36503486","wikidata":"https://www.wikidata.org/wiki/Q11235244","display_name":"Domain (mathematical analysis)","level":2,"score":0.46941858530044556},{"id":"https://openalex.org/C2779240715","wikidata":"https://www.wikidata.org/wiki/Q333936","display_name":"Nitrile","level":2,"score":0.46568599343299866},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4220086336135864},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.3774172067642212},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.20564165711402893},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.1377391517162323},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C134306372","wikidata":"https://www.wikidata.org/wiki/Q7754","display_name":"Mathematical analysis","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000072417","descriptor_name":"Protein Domains","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001884","descriptor_name":"Bordetella","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D004795","descriptor_name":"Enzyme Stability","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D006836","descriptor_name":"Hydro-Lyases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D006836","descriptor_name":"Hydro-Lyases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D021122","descriptor_name":"Protein Subunits","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1007/s12010-015-1961-z","doi":"https://doi.org/10.1007/s12010-015-1961-z","is_oa":false,"landing_page_url":"https://doi.org/10.1007/s12010-015-1961-z","pdf_url":null,"source":{"id":"https://openalex.org/S153285774","display_name":"Applied Biochemistry and Biotechnology","issn_l":"0273-2289","issn":["0273-2289","1559-0291"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied Biochemistry and Biotechnology","raw_type":"journal-article"},{"id":"pmid:26686500","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/26686500","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied biochemistry and biotechnology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G694388650","display_name":null,"funder_award_id":"21506172","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"}],"funders":[{"id":"https://openalex.org/F4320321001","display_name":"National Natural Science Foundation of China","ror":"https://ror.org/01h0zpd94"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":11,"referenced_works":["https://openalex.org/W1977877548","https://openalex.org/W1978280518","https://openalex.org/W1981691884","https://openalex.org/W2023686356","https://openalex.org/W2040708326","https://openalex.org/W2080817938","https://openalex.org/W2088496676","https://openalex.org/W2088863819","https://openalex.org/W2123509270","https://openalex.org/W2149020682","https://openalex.org/W3192366276"],"related_works":["https://openalex.org/W2814851942","https://openalex.org/W2770014334","https://openalex.org/W4294846956","https://openalex.org/W2123509270","https://openalex.org/W4226318330","https://openalex.org/W2080817938","https://openalex.org/W3138355099","https://openalex.org/W2748838133","https://openalex.org/W3093073693","https://openalex.org/W4400699405"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":2},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}