{"id":"https://openalex.org/W2263806019","doi":"https://doi.org/10.2144/000114280","title":"Rapid Method for the Isolation of Mammalian Sperm DNA","display_name":"Rapid Method for the Isolation of Mammalian Sperm DNA","publication_year":2015,"publication_date":"2015-06-01","ids":{"openalex":"https://openalex.org/W2263806019","doi":"https://doi.org/10.2144/000114280","mag":"2263806019","pmid":"https://pubmed.ncbi.nlm.nih.gov/26054765","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/4486329"},"language":"en","primary_location":{"id":"doi:10.2144/000114280","doi":"https://doi.org/10.2144/000114280","is_oa":true,"landing_page_url":"https://doi.org/10.2144/000114280","pdf_url":null,"source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.2144/000114280","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5071832001","display_name":"Haotian Wu","orcid":"https://orcid.org/0000-0002-6271-0249","observed_orcids":["https://orcid.org/0000-0002-6271-0249","https://orcid.org/0000-0002-4765-1623","https://orcid.org/0000-0003-2137-6907"]},"institutions":[{"id":"https://openalex.org/I24603500","display_name":"University of Massachusetts Amherst","ror":"https://ror.org/0072zz521","country_code":"US","type":"education","lineage":["https://openalex.org/I24603500"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Haotian Wu","raw_affiliation_strings":["Department of Environmental Health Sciences, School of Public Health and Health Sciences,University of Massachusetts Amherst,\r          Amherst,\r          MA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Environmental Health Sciences, School of Public Health and Health Sciences,University of Massachusetts Amherst,\r          Amherst,\r          MA","institution_ids":["https://openalex.org/I24603500"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5003528718","display_name":"Matthew de Gannes","orcid":"https://orcid.org/0000-0001-8265-3539","observed_orcids":["https://orcid.org/0000-0001-8265-3539"]},"institutions":[{"id":"https://openalex.org/I24603500","display_name":"University of Massachusetts Amherst","ror":"https://ror.org/0072zz521","country_code":"US","type":"education","lineage":["https://openalex.org/I24603500"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Matthew K. de Gannes","raw_affiliation_strings":["Department of Environmental Health Sciences, School of Public Health and Health Sciences,University of Massachusetts Amherst,\r          Amherst,\r          MA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Environmental Health Sciences, School of Public Health and Health Sciences,University of Massachusetts Amherst,\r          Amherst,\r          MA","institution_ids":["https://openalex.org/I24603500"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031341017","display_name":"Gianna Luchetti","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I24603500","display_name":"University of Massachusetts Amherst","ror":"https://ror.org/0072zz521","country_code":"US","type":"education","lineage":["https://openalex.org/I24603500"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Gianna Luchetti","raw_affiliation_strings":["Department of Environmental Health Sciences, School of Public Health and Health Sciences,University of Massachusetts Amherst,\r          Amherst,\r          MA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Environmental Health Sciences, School of Public Health and Health Sciences,University of Massachusetts Amherst,\r          Amherst,\r          MA","institution_ids":["https://openalex.org/I24603500"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5033173769","display_name":"Jonathan Richard Pilsner","orcid":"https://orcid.org/0000-0002-4541-6267","observed_orcids":["https://orcid.org/0000-0002-4541-6267"]},"institutions":[{"id":"https://openalex.org/I24603500","display_name":"University of Massachusetts Amherst","ror":"https://ror.org/0072zz521","country_code":"US","type":"education","lineage":["https://openalex.org/I24603500"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J.Richard Pilsner","raw_affiliation_strings":["Department of Environmental Health Sciences, School of Public Health and Health Sciences,University of Massachusetts Amherst,\r          Amherst,\r          MA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Environmental Health Sciences, School of Public Health and Health Sciences,University of Massachusetts Amherst,\r          Amherst,\r          MA","institution_ids":["https://openalex.org/I24603500"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I24603500"],"apc_list":{"value":1415,"currency":"USD","value_usd":1415},"apc_paid":{"value":1415,"currency":"USD","value_usd":1415},"fwci":3.2696,"has_fulltext":false,"cited_by_count":70,"citation_normalized_percentile":{"value":0.91499061,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"58","issue":"6","first_page":"293","last_page":"300"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.9580000042915344,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.9580000042915344,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10269","display_name":"Epigenetics and DNA Methylation","score":0.022600000724196434,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.007799999788403511,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/spermatozoa","display_name":"spermatozoa","score":0.8270000219345093},{"id":"https://openalex.org/keywords/dna-methylation","display_name":"DNA methylation","score":0.8970000147819519},{"id":"https://openalex.org/keywords/genomic-imprinting","display_name":"genomic imprinting","score":0.9549999833106995},{"id":"https://openalex.org/keywords/human-spermatozoa","display_name":"human spermatozoa","score":0.4449999928474426},{"id":"https://openalex.org/keywords/mammalian-sperm","display_name":"mammalian sperm","score":0.6439999938011169},{"id":"https://openalex.org/keywords/sample-storage","display_name":"sample storage","score":0.45899999141693115}],"concepts":[{"id":"https://openalex.org/C2775941552","wikidata":"https://www.wikidata.org/wiki/Q25212305","display_name":"Isolation (microbiology)","level":2,"score":0.6900638341903687},{"id":"https://openalex.org/C2781087480","wikidata":"https://www.wikidata.org/wiki/Q17145","display_name":"Sperm","level":2,"score":0.64336097240448},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6193684935569763},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.6027036905288696},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.4501186013221741},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3339729309082031},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.13242489099502563}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002469","descriptor_name":"Cell Separation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D004586","descriptor_name":"Electrophoresis","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010720","descriptor_name":"Phosphines","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012822","descriptor_name":"Silicon Dioxide","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013094","descriptor_name":"Spermatozoa","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D019163","descriptor_name":"Reducing Agents","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D019175","descriptor_name":"DNA Methylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":5,"locations":[{"id":"doi:10.2144/000114280","doi":"https://doi.org/10.2144/000114280","is_oa":true,"landing_page_url":"https://doi.org/10.2144/000114280","pdf_url":null,"source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},{"id":"pmid:26054765","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/26054765","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"Journal Article"},{"id":"pmh:oai:europepmc.org:3453829","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4486329","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:4486329","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4486329","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Biotechniques","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:10287e361c9c4665986c5b6ae41a4c02","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/10287e361c9c4665986c5b6ae41a4c02","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BioTechniques, Vol 58, Iss 6, Pp 293-300 (2015)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.2144/000114280","doi":"https://doi.org/10.2144/000114280","is_oa":true,"landing_page_url":"https://doi.org/10.2144/000114280","pdf_url":null,"source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G5847286372","display_name":"Phthalate Exposure, Sperm DNA Methylation and Early-life Development","funder_award_id":"K22ES023085","funder_id":"https://openalex.org/F4320337361","funder_display_name":"National Institute of Environmental Health Sciences"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337361","display_name":"National Institute of Environmental Health Sciences","ror":"https://ror.org/00j4k1h63"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":30,"referenced_works":["https://openalex.org/W11909344","https://openalex.org/W65623264","https://openalex.org/W1553479965","https://openalex.org/W1964444099","https://openalex.org/W1990195106","https://openalex.org/W1995239849","https://openalex.org/W2012603112","https://openalex.org/W2020331089","https://openalex.org/W2024663595","https://openalex.org/W2027889155","https://openalex.org/W2037365087","https://openalex.org/W2037818631","https://openalex.org/W2045896091","https://openalex.org/W2048918307","https://openalex.org/W2064801165","https://openalex.org/W2073122969","https://openalex.org/W2103336074","https://openalex.org/W2103410123","https://openalex.org/W2116046187","https://openalex.org/W2131104106","https://openalex.org/W2133988692","https://openalex.org/W2134726260","https://openalex.org/W2137291580","https://openalex.org/W2138116401","https://openalex.org/W2150663160","https://openalex.org/W2151372165","https://openalex.org/W2152343110","https://openalex.org/W2157231577","https://openalex.org/W2163670586","https://openalex.org/W2397837047"],"related_works":["https://openalex.org/W2157231577","https://openalex.org/W95658002","https://openalex.org/W4244527569","https://openalex.org/W2340274347","https://openalex.org/W65623264","https://openalex.org/W1995239849","https://openalex.org/W2781378988","https://openalex.org/W4403998693","https://openalex.org/W4311358967","https://openalex.org/W4297992029"],"abstract_inverted_index":{"The":[0],"unique":[1],"DNA":[2,10,73,82,109],"packaging":[3],"of":[4,57,99,159,173],"spermatozoa":[5],"renders":[6],"them":[7],"resistant":[8],"to":[9],"isolation":[11,89,95],"techniques":[12],"used":[13],"for":[14,32,48,120,165,180],"somatic":[15],"cells,":[16],"requiring":[17],"alternative":[18],"methods":[19],"that":[20],"are":[21,140,155],"slow":[22],"and":[23,94,148,161],"labor":[24],"intensive.":[25],"Here":[26],"we":[27],"present":[28],"a":[29],"rapid":[30],"method":[31,69,176],"isolating":[33],"high-quality":[34,72],"sperm":[35,39],"DNA.":[36],"Isolated":[37],"human":[38],"cells":[40],"were":[41],"homogenized":[42],"with":[43,63],"0.2":[44],"mm":[45],"steel":[46],"beads":[47],"5":[49],"min":[50],"at":[51,102,116,132,150],"room":[52,103,133,151],"temperature":[53,104],"in":[54],"the":[55,142,171],"presence":[56],"guanidine":[58],"thiocyanate":[59],"lysis":[60],"buffer":[61],"supplemented":[62],"50":[64],"mM":[65],"tris(2-carboxyethyl)phosphine":[66],"(TCEP).":[67],"Our":[68,124],"yielded":[70],">90%":[71],"using":[74],"3":[75],"different":[76],"commercially":[77],"available":[78],"silica-based":[79],"spin":[80],"columns.":[81],"yields":[83],"did":[84],"not":[85],"differ":[86],"between":[87],"immediate":[88],"(2.84":[90],"\u00b1":[91,106],"0.04":[92],"pg/cell)":[93],"after":[96],"2":[97],"weeks":[98],"homogenate":[100],"storage":[101,158],"(2.91":[105],"0.13":[107],"pg/cell).":[108],"methylation":[110,114],"analyses":[111],"revealed":[112],"similar":[113],"levels":[115],"both":[117],"time":[118],"points":[119],"three":[121],"imprinted":[122],"loci.":[123],"protocol":[125],"has":[126],"many":[127],"advantages:":[128],"it":[129,162],"is":[130,146,163],"conducted":[131],"temperature;":[134,152],"lengthy":[135],"proteinase":[136],"K":[137],"(ProK)":[138],"digestions":[139],"eliminated;":[141],"reducing":[143],"agent,":[144],"TCEP,":[145],"odorless":[147],"stable":[149],"nucleic":[153],"acids":[154],"stabilized,":[156],"allowing":[157],"homogenate;":[160],"adaptable":[164],"other":[166],"mammalian":[167],"species.":[168],"Taken":[169],"together,":[170],"benefits":[172],"our":[174],"improved":[175],"have":[177],"important":[178],"implications":[179],"settings":[181],"where":[182],"sample":[183],"processing":[184],"constraints":[185],"exist.":[186]},"counts_by_year":[{"year":2026,"cited_by_count":6},{"year":2025,"cited_by_count":7},{"year":2024,"cited_by_count":13},{"year":2023,"cited_by_count":8},{"year":2022,"cited_by_count":8},{"year":2021,"cited_by_count":8},{"year":2020,"cited_by_count":4},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":6},{"year":2017,"cited_by_count":5},{"year":2015,"cited_by_count":1}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}