{"id":"https://openalex.org/W2256973399","doi":null,"title":"[Purification and characterization of trichokirin-S1, a novel ribosome-inactivating peptide from seeds of Trichosanthes kirilowii].","display_name":"[Purification and characterization of trichokirin-S1, a novel ribosome-inactivating peptide from seeds of Trichosanthes kirilowii].","publication_year":2003,"publication_date":"2003-09-01","ids":{"openalex":"https://openalex.org/W2256973399","mag":"2256973399","pmid":"https://pubmed.ncbi.nlm.nih.gov/12958658"},"language":"zh","primary_location":{"id":"pmid:12958658","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12958658","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","raw_type":"English Abstract"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5027386261","display_name":"\u674e\u4e30","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I4210106477","display_name":"Shanghai Institutes for Biological Sciences","ror":"https://ror.org/011rf9d86","country_code":"CN","type":"facility","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210106477"]},{"id":"https://openalex.org/I4210121447","display_name":"Center for Excellence in Molecular Cell Science","ror":"https://ror.org/02rrdvm96","country_code":"CN","type":"facility","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210121447"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Feng Li","raw_affiliation_strings":["Key Laboratory of Proteomics Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Proteomics Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China","institution_ids":["https://openalex.org/I19820366","https://openalex.org/I4210106477","https://openalex.org/I4210121447"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5069171555","display_name":"\u6768\u6b23\u79c0","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Xin-Xiu Yang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5082692266","display_name":"\u80e1\u7ef4\u56fd","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Wei-Guo Hu","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035703099","display_name":"\u590f\u6052\u4f20","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Hen-Chuan Xia","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006000658","display_name":"\u674e\u81fb","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Zhen Li","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5087951106","display_name":"\u5f20\u7956\u4f20","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Zu-Chuan Zhang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.2076,"has_fulltext":false,"cited_by_count":10,"citation_normalized_percentile":{"value":0.5211004,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":93},"biblio":{"volume":"35","issue":"9","first_page":"841","last_page":"6"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12477","display_name":"Toxin Mechanisms and Immunotoxins","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12477","display_name":"Toxin Mechanisms and Immunotoxins","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11103","display_name":"Antimicrobial Peptides and Activities","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/trichosanthes-kirilowii","display_name":"Trichosanthes kirilowii","score":0.9950000047683716},{"id":"https://openalex.org/keywords/trichosanthin","display_name":"trichosanthin","score":0.8550000190734863},{"id":"https://openalex.org/keywords/protein-purification","display_name":"protein purification","score":0.6800000071525574},{"id":"https://openalex.org/keywords/protein-synthesis-inhibition","display_name":"protein synthesis inhibition","score":0.8199999928474426},{"id":"https://openalex.org/keywords/immunotoxins","display_name":"immunotoxins","score":0.8299999833106995}],"concepts":[{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.33817124366760254}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002474","descriptor_name":"Cell-Free System","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004305","descriptor_name":"Dose-Response Relationship, Drug","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010936","descriptor_name":"Plant Extracts","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D010936","descriptor_name":"Plant Extracts","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D010936","descriptor_name":"Plant Extracts","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D010940","descriptor_name":"Plant Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D010940","descriptor_name":"Plant Proteins","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D010940","descriptor_name":"Plant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011817","descriptor_name":"Rabbits","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012270","descriptor_name":"Ribosomes","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D012270","descriptor_name":"Ribosomes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012639","descriptor_name":"Seeds","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D014176","descriptor_name":"Protein Biosynthesis","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D019032","descriptor_name":"Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020539","descriptor_name":"Sequence Analysis, Protein","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D029778","descriptor_name":"Trichosanthes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"pmid:12958658","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12958658","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","raw_type":"English Abstract"},{"id":"mag:2256973399","doi":null,"is_oa":false,"landing_page_url":"http://www.cqvip.com/QK/90160X/200309/8290528.html","pdf_url":null,"source":{"id":"https://openalex.org/S4306555117","display_name":"\u751f\u7269\u5316\u5b66\u4e0e\u751f\u7269\u7269\u7406\u5b66\u62a5\uff1a\u82f1\u6587\u7248","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"\u751f\u7269\u5316\u5b66\u4e0e\u751f\u7269\u7269\u7406\u5b66\u62a5\uff1a\u82f1\u6587\u7248","raw_type":null},{"id":"mag:2370329606","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12958658","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1135989296","https://openalex.org/W2467641475","https://openalex.org/W1591875435","https://openalex.org/W2037032875","https://openalex.org/W2422578269","https://openalex.org/W2615939731","https://openalex.org/W2062267781","https://openalex.org/W2065367477","https://openalex.org/W2055996570","https://openalex.org/W915421167"],"abstract_inverted_index":{"A":[0],"novel":[1],"peptide":[2],"from":[3],"the":[4,53,58],"seeds":[5],"of":[6,14,57,84,97],"Trichosanthes":[7],"kirilowii,":[8],"trichokirin-S1,":[9],"was":[10,37,52],"purified":[11,66],"by":[12,42],"extraction":[13],"protein":[15,60,74],"body,":[16],"ammonia":[17],"sulfate":[18],"precipitation,":[19],"Blue-gel":[20],"affinity":[21],"chromatography,":[22],"FPLC":[23],"Mono":[24],"S":[25],"ion":[26],"exchange":[27],"chromatography":[28],"and":[29,93],"Superose12":[30],"gel":[31],"filtration":[32],"chromatography.":[33],"Its":[34,46],"molecular":[35],"weight":[36],"determined":[38],"to":[39,49],"be":[40,90],"11,426":[41],"MALDI-TOF":[43],"MS":[44],"analysis.":[45],"reaction":[47],"mechanism":[48],"inactive":[50],"ribosome":[51],"same":[54],"as":[55],"that":[56],"ribosome-inactivating":[59],"trichosanthin,":[61],"a":[62,69,91],"rRNA":[63],"N-glycosidase.":[64],"The":[65],"trichokirin-S1":[67,88],"showed":[68],"strong":[70],"inhibitory":[71],"activity":[72],"on":[73],"synthesis":[75],"in":[76],"cell-free":[77],"rabbit":[78],"reticulocyte":[79],"lysate":[80],"system,":[81],"with":[82],"IC(50)":[83],"0.7":[85],"nmol/L.":[86],"Therefore,":[87],"may":[89],"promising":[92],"efficient":[94],"toxin":[95],"moiety":[96],"immunotoxins.":[98]},"counts_by_year":[{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1}],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2016-06-24T00:00:00"}