{"id":"https://openalex.org/W2255585271","doi":"https://doi.org/10.1038/srep11333","title":"Production of monoclonal antibodies against GPCR using cell-free synthesized GPCR antigen and biotinylated liposome-based interaction assay","display_name":"Production of monoclonal antibodies against GPCR using cell-free synthesized GPCR antigen and biotinylated liposome-based interaction assay","publication_year":2015,"publication_date":"2015-06-10","ids":{"openalex":"https://openalex.org/W2255585271","doi":"https://doi.org/10.1038/srep11333","mag":"2255585271","pmid":"https://pubmed.ncbi.nlm.nih.gov/26061673","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/4462149"},"language":"en","primary_location":{"id":"doi:10.1038/srep11333","doi":"https://doi.org/10.1038/srep11333","is_oa":true,"landing_page_url":"https://doi.org/10.1038/srep11333","pdf_url":"https://www.nature.com/articles/srep11333.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.nature.com/articles/srep11333.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5078618329","display_name":"Hiroyuki Takeda","orcid":"https://orcid.org/0000-0003-1677-8127","observed_orcids":["https://orcid.org/0000-0003-1677-8127"]},"institutions":[{"id":"https://openalex.org/I43545212","display_name":"Ehime University","ror":"https://ror.org/017hkng22","country_code":"JP","type":"education","lineage":["https://openalex.org/I43545212"]},{"id":"https://openalex.org/I4405287542","display_name":"Proteo-Science Center, Ehime University","ror":"https://ror.org/05ffb9p90","country_code":"JP","type":"facility","lineage":["https://openalex.org/I43545212","https://openalex.org/I4405287542"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hiroyuki Takeda","raw_affiliation_strings":["Proteo-Science Center, Ehime University, Ehime 791-8577, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Proteo-Science Center, Ehime University, Ehime 791-8577, Japan","institution_ids":["https://openalex.org/I43545212","https://openalex.org/I4405287542"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112290324","display_name":"Tomio Ogasawara","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I43545212","display_name":"Ehime University","ror":"https://ror.org/017hkng22","country_code":"JP","type":"education","lineage":["https://openalex.org/I43545212"]},{"id":"https://openalex.org/I4405287542","display_name":"Proteo-Science Center, Ehime University","ror":"https://ror.org/05ffb9p90","country_code":"JP","type":"facility","lineage":["https://openalex.org/I43545212","https://openalex.org/I4405287542"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Tomio Ogasawara","raw_affiliation_strings":["Proteo-Science Center, Ehime University, Ehime 791-8577, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Proteo-Science Center, Ehime University, Ehime 791-8577, Japan","institution_ids":["https://openalex.org/I43545212","https://openalex.org/I4405287542"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077736337","display_name":"Tatsuhiko Ozawa","orcid":"https://orcid.org/0000-0002-3112-452X","observed_orcids":["https://orcid.org/0000-0002-3112-452X"]},"institutions":[{"id":"https://openalex.org/I42766147","display_name":"University of Toyama","ror":"https://ror.org/0445phv87","country_code":"JP","type":"education","lineage":["https://openalex.org/I42766147"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Tatsuhiko Ozawa","raw_affiliation_strings":["Graduate School of Medicine and Pharmaceutical Sciences for Research, University of Toyama, Toyama 930-0194, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Graduate School of Medicine and Pharmaceutical Sciences for Research, University of Toyama, Toyama 930-0194, Japan","institution_ids":["https://openalex.org/I42766147"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027919674","display_name":"Atsushi Muraguchi","orcid":"https://orcid.org/0000-0002-1035-1977","observed_orcids":["https://orcid.org/0000-0002-1035-1977"]},"institutions":[{"id":"https://openalex.org/I42766147","display_name":"University of Toyama","ror":"https://ror.org/0445phv87","country_code":"JP","type":"education","lineage":["https://openalex.org/I42766147"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Atsushi Muraguchi","raw_affiliation_strings":["Graduate School of Medicine and Pharmaceutical Sciences for Research, University of Toyama, Toyama 930-0194, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Graduate School of Medicine and Pharmaceutical Sciences for Research, University of Toyama, Toyama 930-0194, Japan","institution_ids":["https://openalex.org/I42766147"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5071823483","display_name":"Pei-Ju Jih","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Pei-Ju Jih","raw_affiliation_strings":["Abnova (Taiwan) Corporation, Taoyuan County 320, Taiwan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Abnova (Taiwan) Corporation, Taoyuan County 320, Taiwan","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018230010","display_name":"Ryo Morishita","orcid":"https://orcid.org/0000-0002-7763-5712","observed_orcids":["https://orcid.org/0000-0002-7763-5712"]},"institutions":[{"id":"https://openalex.org/I4210089413","display_name":"CellFree Sciences (Japan)","ror":"https://ror.org/004h24333","country_code":"JP","type":"company","lineage":["https://openalex.org/I4210089413"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Ryo Morishita","raw_affiliation_strings":["Cell-Free Sciences Co., Ltd., Ehime 791-8577, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cell-Free Sciences Co., Ltd., Ehime 791-8577, Japan","institution_ids":["https://openalex.org/I4210089413"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5025749036","display_name":"Motokazu Uchigashima","orcid":"https://orcid.org/0000-0002-0878-2233","observed_orcids":["https://orcid.org/0000-0002-0878-2233"]},"institutions":[{"id":"https://openalex.org/I205349734","display_name":"Hokkaido University","ror":"https://ror.org/02e16g702","country_code":"JP","type":"education","lineage":["https://openalex.org/I205349734"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Motokazu Uchigashima","raw_affiliation_strings":["Graduate School of Medicine, Hokkaido University, Hokkaido 060-8638, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Graduate School of Medicine, Hokkaido University, Hokkaido 060-8638, Japan","institution_ids":["https://openalex.org/I205349734"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5053575988","display_name":"Masahiko Watanabe","orcid":"https://orcid.org/0000-0001-9038-0446","observed_orcids":["https://orcid.org/0000-0001-9038-0446"]},"institutions":[{"id":"https://openalex.org/I205349734","display_name":"Hokkaido University","ror":"https://ror.org/02e16g702","country_code":"JP","type":"education","lineage":["https://openalex.org/I205349734"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Masahiko Watanabe","raw_affiliation_strings":["Graduate School of Medicine, Hokkaido University, Hokkaido 060-8638, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Graduate School of Medicine, Hokkaido University, Hokkaido 060-8638, Japan","institution_ids":["https://openalex.org/I205349734"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5011835411","display_name":"Toyoshi Fujimoto","orcid":"https://orcid.org/0000-0002-3601-7977","observed_orcids":["https://orcid.org/0000-0002-3601-7977"]},"institutions":[{"id":"https://openalex.org/I60134161","display_name":"Nagoya University","ror":"https://ror.org/04chrp450","country_code":"JP","type":"education","lineage":["https://openalex.org/I60134161"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Toyoshi Fujimoto","raw_affiliation_strings":["Graduate School of Medicine, Nagoya University, Aichi 466-8550, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Graduate School of Medicine, Nagoya University, Aichi 466-8550, Japan","institution_ids":["https://openalex.org/I60134161"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5003149070","display_name":"Takahiro Iwasaki","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I43545212","display_name":"Ehime University","ror":"https://ror.org/017hkng22","country_code":"JP","type":"education","lineage":["https://openalex.org/I43545212"]},{"id":"https://openalex.org/I4405287542","display_name":"Proteo-Science Center, Ehime University","ror":"https://ror.org/05ffb9p90","country_code":"JP","type":"facility","lineage":["https://openalex.org/I43545212","https://openalex.org/I4405287542"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Takahiro Iwasaki","raw_affiliation_strings":["Proteo-Science Center, Ehime University, Ehime 791-8577, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Proteo-Science Center, Ehime University, Ehime 791-8577, Japan","institution_ids":["https://openalex.org/I43545212","https://openalex.org/I4405287542"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111660653","display_name":"Yaeta Endo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I43545212","display_name":"Ehime University","ror":"https://ror.org/017hkng22","country_code":"JP","type":"education","lineage":["https://openalex.org/I43545212"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yaeta Endo","raw_affiliation_strings":["Institute for the Promotion of Science and Technology, Ehime University, Ehime 791-8577, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for the Promotion of Science and Technology, Ehime University, Ehime 791-8577, Japan","institution_ids":["https://openalex.org/I43545212"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5019414527","display_name":"Tatsuya Sawasaki","orcid":"https://orcid.org/0000-0002-7952-0556","observed_orcids":["https://orcid.org/0000-0002-7952-0556"]},"institutions":[{"id":"https://openalex.org/I43545212","display_name":"Ehime University","ror":"https://ror.org/017hkng22","country_code":"JP","type":"education","lineage":["https://openalex.org/I43545212"]},{"id":"https://openalex.org/I4405287542","display_name":"Proteo-Science Center, Ehime University","ror":"https://ror.org/05ffb9p90","country_code":"JP","type":"facility","lineage":["https://openalex.org/I43545212","https://openalex.org/I4405287542"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Tatsuya Sawasaki","raw_affiliation_strings":["Proteo-Science Center, Ehime University, Ehime 791-8577, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Proteo-Science Center, Ehime University, Ehime 791-8577, Japan","institution_ids":["https://openalex.org/I43545212","https://openalex.org/I4405287542"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":6,"corresponding_author_ids":["https://openalex.org/A5019414527"],"corresponding_institution_ids":["https://openalex.org/I43545212","https://openalex.org/I4405287542"],"apc_list":{"value":2690,"currency":"USD","value_usd":2690},"apc_paid":{"value":2690,"currency":"USD","value_usd":2690},"fwci":3.9226,"has_fulltext":true,"cited_by_count":71,"citation_normalized_percentile":{"value":0.95248376,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":93,"max":99},"biblio":{"volume":"5","issue":"1","first_page":"11333","last_page":"11333"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.8676999807357788,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.8676999807357788,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.12129999697208405,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.0032999999821186066,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/g-protein-coupled-receptors","display_name":"G protein-coupled receptors","score":0.9179999828338623},{"id":"https://openalex.org/keywords/monoclonal-antibodies","display_name":"monoclonal antibodies","score":0.8849999904632568},{"id":"https://openalex.org/keywords/cell-free-protein-synthesis","display_name":"cell-free protein synthesis","score":0.9419999718666077},{"id":"https://openalex.org/keywords/dopamine-d1-receptor","display_name":"dopamine D1 receptor","score":0.8109999895095825},{"id":"https://openalex.org/keywords/growth-hormone-secretagogue-receptor","display_name":"growth hormone secretagogue receptor","score":0.8399999737739563},{"id":"https://openalex.org/keywords/t1r1","display_name":"T1R1","score":0.7400000095367432},{"id":"https://openalex.org/keywords/extracellular-loop-2","display_name":"second extracellular loop","score":0.7630000114440918},{"id":"https://openalex.org/keywords/antibody-selection","display_name":"antibody selection","score":0.46299999952316284}],"concepts":[{"id":"https://openalex.org/C135285700","wikidata":"https://www.wikidata.org/wiki/Q38173","display_name":"G protein-coupled receptor","level":3,"score":0.8599902391433716},{"id":"https://openalex.org/C542903549","wikidata":"https://www.wikidata.org/wiki/Q422248","display_name":"Monoclonal antibody","level":3,"score":0.8045827746391296},{"id":"https://openalex.org/C204628709","wikidata":"https://www.wikidata.org/wiki/Q864854","display_name":"Biotinylation","level":2,"score":0.7578976154327393},{"id":"https://openalex.org/C185154212","wikidata":"https://www.wikidata.org/wiki/Q410454","display_name":"Liposome","level":2,"score":0.6074421405792236},{"id":"https://openalex.org/C195616568","wikidata":"https://www.wikidata.org/wiki/Q128711","display_name":"Epitope","level":3,"score":0.5884228944778442},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.49042221903800964},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.4688105881214142},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.44167065620422363},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.43872272968292236},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.4186924993991852},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.37861257791519165},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3759062588214874},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.35942649841308594},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3386967182159424},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.12829893827438354},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.1110055148601532}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D000915","descriptor_name":"Antibody Affinity","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D000939","descriptor_name":"Epitopes","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D002474","descriptor_name":"Cell-Free System","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004797","descriptor_name":"Enzyme-Linked Immunosorbent Assay","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008081","descriptor_name":"Liposomes","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D011817","descriptor_name":"Rabbits","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000138","qualifier_name":"chemical synthesis","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D017447","descriptor_name":"Receptors, Dopamine D1","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D018604","descriptor_name":"Epitope Mapping","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019921","descriptor_name":"Biotinylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D043562","descriptor_name":"Receptors, G-Protein-Coupled","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D054440","descriptor_name":"Receptors, Ghrelin","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D058306","descriptor_name":"Receptors, Prostaglandin E, EP1 Subtype","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1038/srep11333","doi":"https://doi.org/10.1038/srep11333","is_oa":true,"landing_page_url":"https://doi.org/10.1038/srep11333","pdf_url":"https://www.nature.com/articles/srep11333.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:4462149","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4462149","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Sci Rep","raw_type":"Text"},{"id":"pmid:26061673","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/26061673","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific reports","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1038/srep11333","doi":"https://doi.org/10.1038/srep11333","is_oa":true,"landing_page_url":"https://doi.org/10.1038/srep11333","pdf_url":"https://www.nature.com/articles/srep11333.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.6240000128746033}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1421616179","display_name":"Cell biological studies on membrane lipid distribution and dynamics","funder_award_id":"15H02500","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G1756435240","display_name":"Development of assays and antibodies for functional analysis of taste receptors.","funder_award_id":"26750365","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G2882303231","display_name":"Basic technology development for Shark nanobody using antigen protein produced by wheat cell-free system","funder_award_id":"26640130","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G3434587448","display_name":"Assay development for analyzing high performance  antibody  selection based on cell-free synthesized GPCR proteoliposome.","funder_award_id":"24710251","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G6477792928","display_name":"Study of the molecular-anatomical basis common to neuromodulatory synapses","funder_award_id":"15K06732","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G8126295772","display_name":"Challenge to establish new preventive and therapeutic methods for rheumatoid arthritis","funder_award_id":"15K09522","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G8326805713","display_name":"Construction of novel methods for monomerization and aggregation of amyloid peptides","funder_award_id":"26750375","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G8596147609","display_name":null,"funder_award_id":"15H05897","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G8795106680","display_name":"Discovery of a novel caspase-1 substrate using a cell-free based protein array","funder_award_id":"25290077","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"}],"funders":[{"id":"https://openalex.org/F4320320912","display_name":"Ministry of Education, Culture, Sports, Science and Technology","ror":"https://ror.org/048rj2z13"},{"id":"https://openalex.org/F4320334764","display_name":"Japan Society for the Promotion of Science","ror":"https://ror.org/00hhkn466"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2255585271.pdf","grobid_xml":"https://content.openalex.org/works/W2255585271.grobid-xml"},"referenced_works_count":40,"referenced_works":["https://openalex.org/W1547923480","https://openalex.org/W1585331224","https://openalex.org/W1970613671","https://openalex.org/W1970663569","https://openalex.org/W1971500138","https://openalex.org/W1981307309","https://openalex.org/W1984767654","https://openalex.org/W1987959660","https://openalex.org/W1990440524","https://openalex.org/W1992496728","https://openalex.org/W1994077336","https://openalex.org/W1998715722","https://openalex.org/W2001725041","https://openalex.org/W2005174659","https://openalex.org/W2006719424","https://openalex.org/W2013833085","https://openalex.org/W2015266107","https://openalex.org/W2020601776","https://openalex.org/W2022047402","https://openalex.org/W2027425640","https://openalex.org/W2028820426","https://openalex.org/W2035171463","https://openalex.org/W2036183103","https://openalex.org/W2042525343","https://openalex.org/W2043582503","https://openalex.org/W2046849514","https://openalex.org/W2074370114","https://openalex.org/W2075262579","https://openalex.org/W2089764479","https://openalex.org/W2089896750","https://openalex.org/W2092083087","https://openalex.org/W2104477830","https://openalex.org/W2105852442","https://openalex.org/W2108222684","https://openalex.org/W2123061071","https://openalex.org/W2123491442","https://openalex.org/W2148404862","https://openalex.org/W2150238414","https://openalex.org/W2157663874","https://openalex.org/W2160854785"],"related_works":["https://openalex.org/W2740185469","https://openalex.org/W7171495775","https://openalex.org/W3021913813","https://openalex.org/W7160832252","https://openalex.org/W4309862447","https://openalex.org/W7171510873","https://openalex.org/W2475348408","https://openalex.org/W1996175175","https://openalex.org/W2084327277","https://openalex.org/W4367839798"],"abstract_inverted_index":{"G-protein-coupled":[0],"receptors":[1],"(GPCRs)":[2],"are":[3,78],"one":[4],"of":[5,23,39,45,59,76,106,125,171,184],"the":[6,57,172],"most":[7],"important":[8],"drug":[9],"targets,":[10],"and":[11,43,69,86,99,117,127,144,154,177],"anti-GPCR":[12],"monoclonal":[13],"antibody":[14,116],"(mAb)":[15],"is":[16,27,192],"an":[17],"essential":[18],"tool":[19],"for":[20,56,102,194],"functional":[21],"analysis":[22],"GPCRs.":[24],"However,":[25],"it":[26],"very":[28],"difficult":[29],"to":[30,35,148],"develop":[31],"GPCR-specific":[32],"mAbs":[33,60,139,153,157,173,179],"due":[34],"difficulties":[36],"in":[37],"production":[38,58],"recombinant":[40],"GPCR":[41,62,195],"antigens,":[42],"lack":[44],"efficient":[46],"mAb":[47,196],"screening":[48],"method.":[49],"Here":[50],"we":[51,109,136],"describe":[52],"a":[53],"novel":[54],"approach":[55,191],"against":[61,140],"using":[63,80],"two":[64,178],"original":[65],"methods,":[66],"bilayer-dialysis":[67,90],"method":[68],"biotinylated":[70],"liposome-based":[71],"interaction":[72,114],"assay":[73],"(BiLIA),":[74],"both":[75],"which":[77,160],"developed":[79],"wheat":[81],"cell-free":[82],"protein":[83,119],"synthesis":[84],"system":[85],"liposome":[87],"technology.":[88],"Using":[89,133],"method,":[91],"various":[92],"GPCRs":[93],"were":[94,158,174],"successfully":[95,137],"synthesized":[96],"with":[97,165],"quality":[98],"quantity":[100],"sufficient":[101],"immunization.":[103],"For":[104],"selection":[105],"specific":[107],"mAb,":[108,150,176],"designed":[110],"BiLIA":[111,122],"that":[112,189],"detects":[113],"between":[115],"membrane":[118],"on":[120],"liposome.":[121],"prevented":[123],"denaturation":[124],"GPCR,":[126],"then":[128],"preferably":[129],"selected":[130],"conformation-sensitive":[131,175],"antibodies.":[132],"this":[134,190],"approach,":[135],"obtained":[138,159],"DRD1,":[141],"GHSR,":[142],"PTGER1":[143],"T1R1.":[145],"With":[146],"respect":[147],"DRD1":[149,164],"36":[151],"mouse":[152],"6":[155],"rabbit":[156],"specifically":[161],"recognized":[162,180],"native":[163],"high":[166],"affinity.":[167],"Among":[168],"them,":[169],"half":[170],"extracellular":[181],"loop":[182],"2":[183],"DRD1.":[185],"These":[186],"results":[187],"indicated":[188],"useful":[193],"production.":[197]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":7},{"year":2024,"cited_by_count":6},{"year":2023,"cited_by_count":6},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":4},{"year":2020,"cited_by_count":7},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":12},{"year":2017,"cited_by_count":11},{"year":2016,"cited_by_count":9},{"year":2015,"cited_by_count":2}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}
