{"id":"https://openalex.org/W2253377341","doi":null,"title":"Intein Engineering for Protien Hydrogel Synthesis and Protein Purification","display_name":"Intein Engineering for Protien Hydrogel Synthesis and Protein Purification","publication_year":2013,"publication_date":"2013-11-26","ids":{"openalex":"https://openalex.org/W2253377341","mag":"2253377341"},"language":"en","primary_location":{"id":"pmh:oai:oaktrust.library.tamu.edu:1969.1/151784","doi":null,"is_oa":true,"landing_page_url":"https://hdl.handle.net/1969.1/151784","pdf_url":"https://hdl.handle.net/1969.1/151784","source":{"id":"https://openalex.org/S4306400291","display_name":"OakTrust (Texas A&M University Libraries)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I91045830","host_organization_name":"Texas A&M University","host_organization_lineage":["https://openalex.org/I91045830"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://hdl.handle.net/1969.1/151784","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101879321","display_name":"Miguel A. Ramirez","orcid":"https://orcid.org/0000-0002-9262-8000","observed_orcids":["https://orcid.org/0000-0002-9262-8000"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Miguel A. Ramirez","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-9262-8000","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5101879321"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.0006000000284984708,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/intein","display_name":"intein","score":0.9729999899864197},{"id":"https://openalex.org/keywords/protein-hydrogels","display_name":"protein hydrogels","score":0.890999972820282},{"id":"https://openalex.org/keywords/protein-immobilization","display_name":"protein immobilization","score":0.9330000281333923},{"id":"https://openalex.org/keywords/nostoc-punctiforme","display_name":"Nostoc punctiforme","score":0.9800000190734863},{"id":"https://openalex.org/keywords/protein-trans-splicing","display_name":"protein trans-splicing","score":0.7820000052452087},{"id":"https://openalex.org/keywords/c-terminal-cleavage","display_name":"C-terminal cleavage","score":0.7950000166893005},{"id":"https://openalex.org/keywords/enzyme-immobilization","display_name":"enzyme immobilization","score":0.6620000004768372},{"id":"https://openalex.org/keywords/enzymatic-fuel-cells","display_name":"enzymatic biofuel cells","score":0.7900000214576721},{"id":"https://openalex.org/keywords/disulfide-bond-formation","display_name":"disulfide bond formation","score":0.746999979019165}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5798823237419128},{"id":"https://openalex.org/C2775954774","wikidata":"https://www.wikidata.org/wiki/Q899401","display_name":"Intein","level":5,"score":0.5757175087928772},{"id":"https://openalex.org/C147816474","wikidata":"https://www.wikidata.org/wiki/Q169525","display_name":"Protein engineering","level":3,"score":0.4504956007003784},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.3994792103767395},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3508915305137634},{"id":"https://openalex.org/C54458228","wikidata":"https://www.wikidata.org/wiki/Q237218","display_name":"RNA splicing","level":4,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.0},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:oaktrust.library.tamu.edu:1969.1/151784","doi":null,"is_oa":true,"landing_page_url":"https://hdl.handle.net/1969.1/151784","pdf_url":"https://hdl.handle.net/1969.1/151784","source":{"id":"https://openalex.org/S4306400291","display_name":"OakTrust (Texas A&M University Libraries)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I91045830","host_organization_name":"Texas A&M University","host_organization_lineage":["https://openalex.org/I91045830"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},{"id":"mag:2253377341","doi":null,"is_oa":false,"landing_page_url":"http://oaktrust.library.tamu.edu/bitstream/handle/1969.1/151784/RAMIREZ-DISSERTATION-2013.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:oaktrust.library.tamu.edu:1969.1/151784","doi":null,"is_oa":true,"landing_page_url":"https://hdl.handle.net/1969.1/151784","pdf_url":"https://hdl.handle.net/1969.1/151784","source":{"id":"https://openalex.org/S4306400291","display_name":"OakTrust (Texas A&M University Libraries)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I91045830","host_organization_name":"Texas A&M University","host_organization_lineage":["https://openalex.org/I91045830"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2253377341.pdf","grobid_xml":"https://content.openalex.org/works/W2253377341.grobid-xml"},"referenced_works_count":217,"referenced_works":["https://openalex.org/W138094741","https://openalex.org/W1512009760","https://openalex.org/W1513208225","https://openalex.org/W1577445029","https://openalex.org/W1592537807","https://openalex.org/W1598301669","https://openalex.org/W1603782424","https://openalex.org/W1652052270","https://openalex.org/W1946972831","https://openalex.org/W1964117837","https://openalex.org/W1964549610","https://openalex.org/W1966070420","https://openalex.org/W1966092511","https://openalex.org/W1967149839","https://openalex.org/W1967287081","https://openalex.org/W1969131096","https://openalex.org/W1969542242","https://openalex.org/W1971100514","https://openalex.org/W1971447541","https://openalex.org/W1973639461","https://openalex.org/W1975895069","https://openalex.org/W1976175443","https://openalex.org/W1977097697","https://openalex.org/W1977743433","https://openalex.org/W1978381255","https://openalex.org/W1981015633","https://openalex.org/W1981076832","https://openalex.org/W1981159786","https://openalex.org/W1981293691","https://openalex.org/W1981864850","https://openalex.org/W1982116729","https://openalex.org/W1982446221","https://openalex.org/W1982656848","https://openalex.org/W1982807734","https://openalex.org/W1983573214","https://openalex.org/W1984921654","https://openalex.org/W1985034470","https://openalex.org/W1985154059","https://openalex.org/W1985367101","https://openalex.org/W1988327710","https://openalex.org/W1989150705","https://openalex.org/W1990952318","https://openalex.org/W1991508224","https://openalex.org/W1991918935","https://openalex.org/W1993016783","https://openalex.org/W1993922236","https://openalex.org/W1994113816","https://openalex.org/W1994181793","https://openalex.org/W1995288466","https://openalex.org/W1996155302","https://openalex.org/W1997119204","https://openalex.org/W1997143754","https://openalex.org/W1998262280","https://openalex.org/W1998834713","https://openalex.org/W1998848630","https://openalex.org/W1999138945","https://openalex.org/W2000250139","https://openalex.org/W2000510381","https://openalex.org/W2000630217","https://openalex.org/W2000687868","https://openalex.org/W2001579035","https://openalex.org/W2001634956","https://openalex.org/W2001684833","https://openalex.org/W2001739179","https://openalex.org/W2002184487","https://openalex.org/W2004785721","https://openalex.org/W2004789826","https://openalex.org/W2007527881","https://openalex.org/W2008841659","https://openalex.org/W2009181661","https://openalex.org/W2009215675","https://openalex.org/W2010301288","https://openalex.org/W2011159612","https://openalex.org/W2012030417","https://openalex.org/W2014849029","https://openalex.org/W2015859382","https://openalex.org/W2017204412","https://openalex.org/W2020613566","https://openalex.org/W2021459287","https://openalex.org/W2021714443","https://openalex.org/W2022113741","https://openalex.org/W2022120527","https://openalex.org/W2025583051","https://openalex.org/W2025686590","https://openalex.org/W2026231072","https://openalex.org/W2027075135","https://openalex.org/W2028073797","https://openalex.org/W2028954462","https://openalex.org/W2029148276","https://openalex.org/W2029667189","https://openalex.org/W2029812059","https://openalex.org/W2030346153","https://openalex.org/W2030437460","https://openalex.org/W2030756659","https://openalex.org/W2031261222","https://openalex.org/W2032736963","https://openalex.org/W2035379837","https://openalex.org/W2036521212","https://openalex.org/W2037955590","https://openalex.org/W2039797753","https://openalex.org/W2041508914","https://openalex.org/W2043451183","https://openalex.org/W2043843363","https://openalex.org/W2045128521","https://openalex.org/W2045795803","https://openalex.org/W2046172734","https://openalex.org/W2046769337","https://openalex.org/W2047944454","https://openalex.org/W2048052722","https://openalex.org/W2048829256","https://openalex.org/W2049711126","https://openalex.org/W2049890972","https://openalex.org/W2050173459","https://openalex.org/W2050801417","https://openalex.org/W2051267614","https://openalex.org/W2051893857","https://openalex.org/W2055462077","https://openalex.org/W2055496019","https://openalex.org/W2057530446","https://openalex.org/W2058933664","https://openalex.org/W2060491938","https://openalex.org/W2060835907","https://openalex.org/W2061088187","https://openalex.org/W2061636126","https://openalex.org/W2062560106","https://openalex.org/W2065716758","https://openalex.org/W2068929850","https://openalex.org/W2069858346","https://openalex.org/W2070698203","https://openalex.org/W2071101400","https://openalex.org/W2071256768","https://openalex.org/W2074276621","https://openalex.org/W2075902092","https://openalex.org/W2077080679","https://openalex.org/W2078669099","https://openalex.org/W2079039462","https://openalex.org/W2081211357","https://openalex.org/W2083368345","https://openalex.org/W2083532852","https://openalex.org/W2083727294","https://openalex.org/W2084327297","https://openalex.org/W2085101776","https://openalex.org/W2086864633","https://openalex.org/W2087147260","https://openalex.org/W2087208805","https://openalex.org/W2087751600","https://openalex.org/W2089465540","https://openalex.org/W2089653670","https://openalex.org/W2090044711","https://openalex.org/W2092473371","https://openalex.org/W2092904335","https://openalex.org/W2094385207","https://openalex.org/W2099439711","https://openalex.org/W2099938627","https://openalex.org/W2104220374","https://openalex.org/W2105199969","https://openalex.org/W2105453906","https://openalex.org/W2106731625","https://openalex.org/W2108128254","https://openalex.org/W2109050242","https://openalex.org/W2109280361","https://openalex.org/W2111433955","https://openalex.org/W2112103769","https://openalex.org/W2112404993","https://openalex.org/W2112988974","https://openalex.org/W2114522205","https://openalex.org/W2115644711","https://openalex.org/W2116423211","https://openalex.org/W2117978571","https://openalex.org/W2119558921","https://openalex.org/W2119651277","https://openalex.org/W2121534263","https://openalex.org/W2124144156","https://openalex.org/W2124684134","https://openalex.org/W2125223059","https://openalex.org/W2125268636","https://openalex.org/W2125665330","https://openalex.org/W2126050310","https://openalex.org/W2126528399","https://openalex.org/W2130554676","https://openalex.org/W2132912202","https://openalex.org/W2133523998","https://openalex.org/W2134622589","https://openalex.org/W2135414262","https://openalex.org/W2137112302","https://openalex.org/W2139749717","https://openalex.org/W2144081123","https://openalex.org/W2144318457","https://openalex.org/W2145035916","https://openalex.org/W2146804295","https://openalex.org/W2147516046","https://openalex.org/W2148313902","https://openalex.org/W2148405034","https://openalex.org/W2148574813","https://openalex.org/W2152194244","https://openalex.org/W2152458717","https://openalex.org/W2154157809","https://openalex.org/W2154232844","https://openalex.org/W2154733173","https://openalex.org/W2155151146","https://openalex.org/W2155614790","https://openalex.org/W2155880545","https://openalex.org/W2156492800","https://openalex.org/W2158928488","https://openalex.org/W2159230616","https://openalex.org/W2160898059","https://openalex.org/W2162967244","https://openalex.org/W2164105551","https://openalex.org/W2164623788","https://openalex.org/W2165694476","https://openalex.org/W2168106956","https://openalex.org/W2168113028","https://openalex.org/W2169000704","https://openalex.org/W2171377206","https://openalex.org/W2171475828","https://openalex.org/W2324165416","https://openalex.org/W2335760320"],"related_works":["https://openalex.org/W2335760320","https://openalex.org/W4200539326","https://openalex.org/W2529109291","https://openalex.org/W7100211623","https://openalex.org/W2997830655","https://openalex.org/W3001924496","https://openalex.org/W2121482133","https://openalex.org/W2389404962","https://openalex.org/W2790103409","https://openalex.org/W2400838500"],"abstract_inverted_index":{"Inteins":[0],"are":[1,126,174,200],"proteins":[2,17],"encoded":[3],"within":[4],"a":[5,19,164,228],"precursor":[6],"gene":[7],"that":[8],"excise":[9],"themselves":[10],"after":[11],"translation":[12],"and":[13,43,53,80,88,119,169,173,199,225],"ligate":[14],"the":[15,41,75,91,98,203,209,214,256],"surrounding":[16],"with":[18,176],"peptide":[20],"bond.":[21],"Since":[22],"their":[23],"discovery":[24],"two":[25],"decades":[26],"ago,":[27],"many":[28],"inteins":[29],"have":[30],"been":[31,113],"engineered":[32,81,213],"for":[33,48,74,108,202,233,262],"various":[34],"biotechnology":[35],"applications.":[36],"This":[37],"dissertation":[38],"focuses":[39],"on":[40],"use":[42],"development":[44],"of":[45,77,94,128,167,205,242],"intein-based":[46],"technologies":[47],"applications":[49],"in":[50,90,133,195,250],"protein":[51,71,78,103,109,117,130,142,151,157,181,235,245,263],"purification":[52,93,236,241],"immobilization.":[54,110],"The":[55,154],"highly":[56],"efficient":[57,240],"naturally":[58],"split":[59,229],"DnaE":[60,216],"intein":[61,217,230,260],"from":[62,187,246],"Nostoc":[63],"punctiforme":[64],"(Npu":[65],"DnaE)":[66],"was":[67],"incorporated":[68],"into":[69],"synthetic":[70],"building":[72,152],"blocks":[73],"synthesis":[76],"hydrogels,":[79],"to":[82,115,218],"catalyze":[83,219],"rapid":[84,92,220],"C-terminal":[85,222],"cleavage":[86,223],"reaction":[87,224],"used":[89,194],"tag-less":[95,243],"protein.":[96],"\\n\\nIn":[97,208],"first":[99],"application,":[100,211],"we":[101,212],"developed":[102,227],"hydrogels":[104,125,158,182],"as":[105],"general":[106],"scaffolds":[107],"Immobilization":[111],"has":[112],"shown":[114],"increase":[116],"stability":[118,162],"facilitate":[120],"enzyme":[121],"recovery-and-recycle":[122],"tasks.":[123],"These":[124,179],"composed":[127],"artificial":[129,156],"building-blocks":[131],"expressed":[132],"bacterial":[134],"hosts.":[135],"Hydrogel":[136],"gelation":[137],"is":[138],"catalyzed":[139],"by":[140],"intein-mediated":[141],"trans-splicing":[143],"reactions":[144],"or":[145],"disulfide":[146],"bond":[147],"formation":[148],"between":[149],"different":[150],"blocks.":[153],"resulting":[155],"possess":[159],"high":[160],"solution":[161],"at":[163],"wide":[165],"range":[166],"pHs":[168],"temperatures,":[170],"undergo":[171],"shear-thinning,":[172],"compatible":[175],"organic":[177,188],"solvents.":[178],"self-assembled":[180],"can":[183],"protect":[184],"immobilized":[185],"enzymes":[186],"solvent":[189],"denaturation":[190],"during":[191],"biosynthesis,":[192],"be":[193],"enzymatic":[196],"biofuel":[197],"cells,":[198],"suitable":[201],"immobilization":[204],"multiple":[206],"enzymes.":[207],"second":[210],"Npu":[215],"thio-induced":[221],"subsequently":[226],"mediated":[231],"technology":[232,261],"recombinant":[234,244],"(SIRP).":[237],"SIRP":[238],"enables":[239],"E.":[247],"coli":[248],"lysate":[249],"less":[251],"than":[252],"1":[253],"hour":[254],"\u2013":[255],"hitherto":[257],"fastest":[258],"reported":[259],"purification.":[264]},"counts_by_year":[],"updated_date":"2026-08-31T07:19:18.363097","created_date":"2016-06-24T00:00:00"}
