{"id":"https://openalex.org/W2247338582","doi":"https://doi.org/10.1182/blood.v124.21.4332.4332","title":"Activation of OCT4 Enhances Ex Vivo Expansion of Phenotypically Defined and Functionally Engraftable Human Cord Blood Hematopoietic Stem and Progenitor Cells By Regulating HOXB4 Expression","display_name":"Activation of OCT4 Enhances Ex Vivo Expansion of Phenotypically Defined and Functionally Engraftable Human Cord Blood Hematopoietic Stem and Progenitor Cells By Regulating HOXB4 Expression","publication_year":2014,"publication_date":"2014-12-06","ids":{"openalex":"https://openalex.org/W2247338582","doi":"https://doi.org/10.1182/blood.v124.21.4332.4332","mag":"2247338582"},"language":"en","primary_location":{"id":"doi:10.1182/blood.v124.21.4332.4332","doi":"https://doi.org/10.1182/blood.v124.21.4332.4332","is_oa":false,"landing_page_url":"https://doi.org/10.1182/blood.v124.21.4332.4332","pdf_url":null,"source":{"id":"https://openalex.org/S200071133","display_name":"Blood","issn_l":"0006-4971","issn":["0006-4971","1528-0020"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Blood","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5051329739","display_name":"Xinxin Huang","orcid":"https://orcid.org/0000-0002-4298-4859","observed_orcids":["https://orcid.org/0000-0002-4298-4859"]},"institutions":[{"id":"https://openalex.org/I4394709130","display_name":"Indiana University School of Medicine","ror":"https://ror.org/02ets8c94","country_code":"US","type":"education","lineage":["https://openalex.org/I4394709130","https://openalex.org/I592451"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Xinxin Huang","raw_affiliation_strings":["Indiana University School of Medicine, Indianapolis, IN"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Indiana University School of Medicine, Indianapolis, IN","institution_ids":["https://openalex.org/I4394709130"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5034118912","display_name":"Scott H. Cooper","orcid":"https://orcid.org/0000-0001-9091-8269","observed_orcids":["https://orcid.org/0000-0001-9091-8269"]},"institutions":[{"id":"https://openalex.org/I4394709130","display_name":"Indiana University School of Medicine","ror":"https://ror.org/02ets8c94","country_code":"US","type":"education","lineage":["https://openalex.org/I4394709130","https://openalex.org/I592451"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Scott Cooper","raw_affiliation_strings":["Indiana University School of Medicine, Indianapolis, IN"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Indiana University School of Medicine, Indianapolis, IN","institution_ids":["https://openalex.org/I4394709130"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5038161052","display_name":"Hal E. Broxmeyer","orcid":"https://orcid.org/0000-0001-6575-0476","observed_orcids":["https://orcid.org/0000-0001-6575-0476"]},"institutions":[{"id":"https://openalex.org/I4394709130","display_name":"Indiana University School of Medicine","ror":"https://ror.org/02ets8c94","country_code":"US","type":"education","lineage":["https://openalex.org/I4394709130","https://openalex.org/I592451"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Hal E. Broxmeyer","raw_affiliation_strings":["Indiana University School of Medicine, Indianapolis, IN"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Indiana University School of Medicine, Indianapolis, IN","institution_ids":["https://openalex.org/I4394709130"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5051329739"],"corresponding_institution_ids":["https://openalex.org/I4394709130"],"apc_list":{"value":5500,"currency":"USD","value_usd":5500},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.18837004,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"124","issue":"21","first_page":"4332","last_page":"4332"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10345","display_name":"Hematopoietic Stem Cell Transplantation","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10345","display_name":"Hematopoietic Stem Cell Transplantation","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10176","display_name":"Mesenchymal stem cell research","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/2716","display_name":"Genetics (clinical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/oct4","display_name":"Oct4","score":0.9470000267028809},{"id":"https://openalex.org/keywords/cord-blood-hematopoietic-stem-cells","display_name":"cord blood hematopoietic stem cells","score":0.8209999799728394},{"id":"https://openalex.org/keywords/ex-vivo-expansion","display_name":"ex vivo expansion","score":0.7789999842643738},{"id":"https://openalex.org/keywords/hoxb4","display_name":"HOXB4","score":0.9380000233650208},{"id":"https://openalex.org/keywords/hematopoietic-progenitor-cells","display_name":"hematopoietic progenitor cells","score":0.8709999918937683},{"id":"https://openalex.org/keywords/allogeneic-hematopoietic-stem-cell-transplantation","display_name":"allogeneic hematopoietic stem cell transplantation","score":0.8479999899864197},{"id":"https://openalex.org/keywords/cd34-cells","display_name":"CD34+ cells","score":0.7310000061988831},{"id":"https://openalex.org/keywords/cfu-gemm","display_name":"CFU-GEMM","score":0.671999990940094},{"id":"https://openalex.org/keywords/transcriptional-regulation","display_name":"transcriptional regulation","score":0.460999995470047},{"id":"https://openalex.org/keywords/hematopoietic-stem-cell-niche","display_name":"hematopoietic niche","score":0.4740000069141388},{"id":"https://openalex.org/keywords/nsg-mice","display_name":"NSG mice","score":0.35899999737739563},{"id":"https://openalex.org/keywords/cytokine-cocktail","display_name":"cytokine cocktail","score":0.37700000405311584}],"concepts":[{"id":"https://openalex.org/C26291073","wikidata":"https://www.wikidata.org/wiki/Q310484","display_name":"Ex vivo","level":3,"score":0.7245553731918335},{"id":"https://openalex.org/C201750760","wikidata":"https://www.wikidata.org/wiki/Q1896218","display_name":"Progenitor cell","level":3,"score":0.6594852209091187},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.6540886163711548},{"id":"https://openalex.org/C2780914630","wikidata":"https://www.wikidata.org/wiki/Q914952","display_name":"Cord blood","level":2,"score":0.6363067626953125},{"id":"https://openalex.org/C109159458","wikidata":"https://www.wikidata.org/wiki/Q919283","display_name":"Haematopoiesis","level":3,"score":0.6089897155761719},{"id":"https://openalex.org/C10205521","wikidata":"https://www.wikidata.org/wiki/Q4035578","display_name":"CD34","level":3,"score":0.6060991287231445},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6047694087028503},{"id":"https://openalex.org/C2911091166","wikidata":"https://www.wikidata.org/wiki/Q106419912","display_name":"Transplantation","level":2,"score":0.5431725978851318},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.46270751953125},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.43785330653190613},{"id":"https://openalex.org/C2778086970","wikidata":"https://www.wikidata.org/wiki/Q514525","display_name":"Hematopoietic stem cell","level":4,"score":0.42545604705810547},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3076610267162323},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.24959081411361694},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.17257198691368103},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.14366137981414795},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0719938576221466}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1182/blood.v124.21.4332.4332","doi":"https://doi.org/10.1182/blood.v124.21.4332.4332","is_oa":false,"landing_page_url":"https://doi.org/10.1182/blood.v124.21.4332.4332","pdf_url":null,"source":{"id":"https://openalex.org/S200071133","display_name":"Blood","issn_l":"0006-4971","issn":["0006-4971","1528-0020"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Blood","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.8999999761581421,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1812483684","https://openalex.org/W1974082660","https://openalex.org/W2021550815","https://openalex.org/W1967203530","https://openalex.org/W2122837254","https://openalex.org/W2254073648","https://openalex.org/W7061739853","https://openalex.org/W3026935488","https://openalex.org/W2559478920","https://openalex.org/W2523294549"],"abstract_inverted_index":{"Abstract":[0],"Allogeneic":[1],"hematopoietic":[2,30],"cell":[3,127],"transplantation":[4],"(HCT)":[5],"is":[6,25,77,97,143,354,380,500],"well":[7,43],"established":[8],"as":[9,42,44,279],"a":[10,26,99,116,174,202,355,485],"clinical":[11,86,532],"means":[12,67,105],"to":[13,68,78,106,183,327,361,386,416,444,469,484,538],"treat":[14],"patients":[15,61],"with":[16,62,196,441],"hematologic":[17],"disorders":[18],"and":[19,113,125,148,150,180,224,236,263,305,526],"cancer.":[20],"Human":[21],"cord":[22],"blood":[23],"(CB)":[24],"viable":[27],"source":[28],"of":[29,38,47,59,72,75,110,132,157,166,173,176,211,217,250,276,289,309,323,336,347,366,370,390,395,407,413,426,437,450,457,472,480,491,505,522,534,543,556],"stem":[31],"cells":[32,40,49,83,169,194,301,440,463,496],"(HSC)":[33],"for":[34,57,84,103,198,502],"transplantation.":[35],"However,":[36],"numbers":[37,46,74,216,249],"nucleated":[39],"retrieved,":[41],"limited":[45],"HSC/progenitor":[48],"(HPC)":[50],"present,":[51],"during":[52],"collection":[53],"may":[54],"be":[55,362],"problematic":[56],"treatment":[58,467,537],"adult":[60],"single":[63],"CB":[64,167,192,299,351,438,524],"HCT.":[65],"One":[66],"address":[69],"the":[70,130,155,171,215,287,321,387,393,399,424,454,489,503,515,530],"problem":[71],"limiting":[73],"HSC/HPC":[76],"ex":[79,107,163,184,344,375,417,519,540],"vivo":[80,108,164,185,226,345,376,418,520,541],"expand":[81,186,419],"these":[82],"potential":[85,531],"use.":[87],"While":[88],"progress":[89],"has":[90,513],"been":[91],"made":[92],"in":[93,123,134,145,170,214,225,229,291,298,313,320,373,453,461,475,488,494,508,518],"this":[94],"endeavor,":[95],"there":[96],"still":[98],"clinically":[100],"relevant":[101],"need":[102],"additional":[104],"expansion":[109,346,521,542],"human":[111,187,292,350,523,546],"HSC":[112,135,146,220,325,367,377,493,507,525],"HPC.":[114],"OCT4,":[115],"transcriptional":[117,357],"factor,":[118],"plays":[119],"an":[120,363,553],"essential":[121,364,501],"role":[122,288],"pluripotency":[124],"somatic":[126],"reprogramming,":[128],"however,":[129],"functions":[131],"OCT4":[133,142,290,296,310,337,383,408,451],"are":[136],"largely":[137,429],"unexplored.":[138],"We":[139,189,403,421],"hypothesized":[140,404],"that":[141,191,210,275,307,334,359,382,405,423,465],"involved":[144],"function":[147],"expansion,":[149],"thus":[151],"we":[152,294],"first":[153],"evaluated":[154],"effects":[156],"OAC1":[158,197,245,339,442,466,536],"(Oct4-activating":[159],"compound":[160],"1)":[161],"on":[162,529,552],"culture":[165,436],"CD34+":[168,193,300,439],"presence":[172],"cocktail":[175,213],"cytokines":[177],"(SCF,":[178],"TPO":[179],"Flt3L)":[181],"known":[182],"HSC.":[188,352,420,476,547],"found":[190,306],"treated":[195],"4":[199],"days":[200],"showed":[201],"significant":[203,314,486],"increase":[204,315],"(2.8":[205],"fold":[206,233,239,254,260,270,317,432],"increase,":[207,234,240,255,261,271,318,433],"p<0.01)":[208,235,241,272,319,434],"above":[209,274],"cytokine":[212,277,348,544],"rigorously":[218],"defined":[219],"by":[221,281,338],"phenotype":[222],"(Lin-CD34+CD38-CD45RA-CD90+CD49f+)":[223],"repopulating":[227],"capacity":[228],"both":[230],"primary":[231],"(3.1":[232],"secondary":[237],"(1.9":[238],"recipient":[242],"NSG":[243],"mice.":[244],"also":[246,311],"significantly":[247],"increased":[248,430],"granulocyte/macrophage":[251],"(CFU-GM,":[252],"2.7":[253],"p<0.01),":[256,262],"erythroid":[257],"(BFU-E,":[258],"2.2":[259],"granulocyte,":[264],"erythroid,":[265],"macrophage,":[266],"megakaryocyte":[267],"(CFU-GEMM,":[268],"2.6":[269],"progenitors":[273],"combinations":[278],"determined":[280],"colony":[282],"assays.":[283],"To":[284],"further":[285],"confirm":[286],"HSC,":[293],"performed":[295],"overexpression":[297,308],"using":[302,535],"lentiviral":[303,341],"vectors":[304,342],"resulted":[312,452],"(2.6":[316],"number":[322,490],"phenotypic":[324],"compared":[326,443],"control":[328],"vectors.":[329],"Together,":[330],"our":[331],"data":[332],"indicate":[333],"activation":[335,406],"or":[340,558],"enhances":[343],"stimulated":[349,545],"HOXB4":[353,371,391,414,427,458,473,481,499],"homeobox":[356],"factor":[358],"appears":[360],"regulator":[365],"self-renewal.":[368],"Overexpression":[369],"results":[372],"high-level":[374],"expansion.":[378],"It":[379],"reported":[381],"can":[384],"bind":[385],"promoter":[388],"region":[389],"at":[392],"site":[394],"2952":[396],"bp":[397],"from":[398],"transcription":[400],"start":[401],"point.":[402],"might":[409],"work":[410],"through":[411],"upregulation":[412,471],"expression":[415,425,459,474,482],"observed":[422],"was":[428],"(2.3":[431],"after":[435],"vehicle":[445],"control.":[446],"siRNA":[447],"mediated":[448],"inhibition":[449],"marked":[455],"reduction":[456,487],"(p<0.01)":[460],"OAC1-treated":[462,495,509],"indicating":[464],"lead":[468],"OCT4-mediated":[470],"Consistently,":[477],"siRNA-mediated":[478],"knockdown":[479],"led":[483],"Lin-CD34+CD38-CD45RA-CD90+CD49f+":[492],"(p<0.05),":[497],"suggesting":[498],"generation":[504],"primitive":[506],"cells.":[510],"Our":[511],"study":[512],"identified":[514],"OCT4-HOXB4":[516],"axis":[517],"sheds":[527],"light":[528],"application":[533],"enhance":[539],"Disclosures":[548],"Broxmeyer:":[549],"CordUse:":[550],"Membership":[551],"entity's":[554],"Board":[555],"Directors":[557],"advisory":[559],"committees.":[560]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}