{"id":"https://openalex.org/W2239800698","doi":"https://doi.org/10.1007/978-1-60327-475-3_5","title":"Purification of Ribonucleoproteins Using Peptide-Elutable Antibodies and Other Affinity Techniques","display_name":"Purification of Ribonucleoproteins Using Peptide-Elutable Antibodies and Other Affinity Techniques","publication_year":2008,"publication_date":"2008-01-01","ids":{"openalex":"https://openalex.org/W2239800698","doi":"https://doi.org/10.1007/978-1-60327-475-3_5","mag":"2239800698","pmid":"https://pubmed.ncbi.nlm.nih.gov/18982284"},"language":"en","primary_location":{"id":"doi:10.1007/978-1-60327-475-3_5","doi":"https://doi.org/10.1007/978-1-60327-475-3_5","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-60327-475-3_5","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W589602034","display_name":"RNA-Protein Interaction Protocols"},"type":"book-chapter","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5060853964","display_name":"Scott W. Stevens","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I86519309","display_name":"The University of Texas at Austin","ror":"https://ror.org/00hj54h04","country_code":"US","type":"education","lineage":["https://openalex.org/I86519309"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Scott W. Stevens","raw_affiliation_strings":["Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas, USA","Section in Molecular Genetics and Microbiology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas, USA","institution_ids":["https://openalex.org/I86519309"]},{"raw_affiliation_string":"Section in Molecular Genetics and Microbiology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas, USA","institution_ids":["https://openalex.org/I86519309"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5060853964"],"corresponding_institution_ids":["https://openalex.org/I86519309"],"apc_list":null,"apc_paid":null,"fwci":0.4319,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":{"value":0.67325139,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"488","issue":null,"first_page":"65","last_page":"84"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.7544000148773193,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.7544000148773193,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.07670000195503235,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T13127","display_name":"Biotin and Related Studies","score":0.0544000007212162,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/ribonucleoprotein","display_name":"ribonucleoprotein","score":0.9869999885559082},{"id":"https://openalex.org/keywords/affinity-purification","display_name":"affinity purification","score":0.7620000243186951},{"id":"https://openalex.org/keywords/epitope-tags","display_name":"epitope tags","score":0.9380000233650208},{"id":"https://openalex.org/keywords/saccharomyces-cerevisiae","display_name":"Saccharomyces cerevisiae","score":0.847000002861023},{"id":"https://openalex.org/keywords/protein-rna-complex","display_name":"protein-RNA complex","score":0.5199999809265137}],"concepts":[{"id":"https://openalex.org/C75934600","wikidata":"https://www.wikidata.org/wiki/Q419245","display_name":"Ribonucleoprotein","level":4,"score":0.7727496027946472},{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.5548650026321411},{"id":"https://openalex.org/C179247698","wikidata":"https://www.wikidata.org/wiki/Q382799","display_name":"Affinity chromatography","level":3,"score":0.5521963834762573},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.5440948605537415},{"id":"https://openalex.org/C2777576037","wikidata":"https://www.wikidata.org/wiki/Q719725","display_name":"Saccharomyces cerevisiae","level":3,"score":0.5439465045928955},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.5226790308952332},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4785485863685608},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.46521711349487305},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.45170390605926514},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4429478049278259},{"id":"https://openalex.org/C195616568","wikidata":"https://www.wikidata.org/wiki/Q128711","display_name":"Epitope","level":3,"score":0.42316657304763794},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.40429794788360596},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.3773098587989807},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3471049666404724},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.21341654658317566},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.19759926199913025},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.1614328920841217},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.12267491221427917},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000906","descriptor_name":"Antibodies","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D010455","descriptor_name":"Peptides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012261","descriptor_name":"Ribonucleoproteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012261","descriptor_name":"Ribonucleoproteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D012261","descriptor_name":"Ribonucleoproteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016681","descriptor_name":"Genome, Fungal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1007/978-1-60327-475-3_5","doi":"https://doi.org/10.1007/978-1-60327-475-3_5","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-60327-475-3_5","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},{"id":"pmid:18982284","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18982284","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in molecular biology (Clifton, N.J.)","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":25,"referenced_works":["https://openalex.org/W175807111","https://openalex.org/W1483456869","https://openalex.org/W1525575010","https://openalex.org/W1594218135","https://openalex.org/W1776247902","https://openalex.org/W1873199501","https://openalex.org/W1964313477","https://openalex.org/W1987666378","https://openalex.org/W1992660876","https://openalex.org/W2015816723","https://openalex.org/W2017125983","https://openalex.org/W2026465178","https://openalex.org/W2035398832","https://openalex.org/W2040108963","https://openalex.org/W2044853699","https://openalex.org/W2049438727","https://openalex.org/W2070324964","https://openalex.org/W2094920550","https://openalex.org/W2128635872","https://openalex.org/W2144634347","https://openalex.org/W2146503498","https://openalex.org/W2149676146","https://openalex.org/W2153375374","https://openalex.org/W2210103237","https://openalex.org/W4240065971"],"related_works":["https://openalex.org/W59286015","https://openalex.org/W2106403358","https://openalex.org/W121636211","https://openalex.org/W2069104829","https://openalex.org/W2066740300","https://openalex.org/W2484788967","https://openalex.org/W2052952174","https://openalex.org/W1973223491","https://openalex.org/W2414879650","https://openalex.org/W4320923539"],"abstract_inverted_index":{"Recently":[0],"developed":[1],"affinity":[2,75,112],"purification":[3,23],"methods":[4],"have":[5],"revolutionized":[6],"our":[7],"understanding":[8],"of":[9,13,39,46,51,56,69,80,94,106,127],"the":[10,37,40,47,78,81,107,110],"higher-ordered":[11,49],"structures":[12],"multisubunit,":[14],"often":[15],"low-abundance":[16],"macromolecular":[17],"complexes,":[18],"including":[19,73],"ribonucleoproteins":[20],"(RNPs).":[21],"Often,":[22],"by":[24,134],"classical,":[25],"non-affinity-based":[26],"techniques":[27],"subjects":[28],"salt-labile":[29],"complexes":[30],"to":[31,43,65],"an":[32],"ionic":[33],"strength":[34],"incompatible":[35],"with":[36,124],"integrity":[38],"RNP,":[41],"leading":[42],"a":[44,67,125],"misrepresentation":[45],"true":[48],"structure":[50],"these":[52,86],"complexes.":[53],"A":[54],"family":[55],"plasmids":[57,87],"has":[58],"been":[59],"generated":[60],"that":[61],"can":[62,114],"be":[63,89,115],"used":[64,90],"introduce":[66],"number":[68],"different":[70],"epitope":[71],"tags,":[72,76],"peptide-elutable":[74],"into":[77],"genome":[79],"yeast":[82,99],"Saccharomyces":[83],"cerevisiae.":[84],"Alternatively,":[85],"may":[88],"for":[91,141],"plasmid-borne":[92],"expression":[93],"epitope-tagged":[95],"proteins":[96],"in":[97],"either":[98],"or":[100,150],"Escherichia":[101],"coli.":[102],"The":[103],"gentle":[104],"elution":[105],"complex":[108],"from":[109],"antibody":[111],"matrix":[113],"performed":[116],"at":[117],"4":[118],"degrees":[119],"C":[120],"and":[121,129,139],"is":[122],"compatible":[123],"range":[126],"salt":[128],"pH":[130],"conditions.":[131],"RNPs":[132],"purified":[133],"this":[135],"method":[136],"are":[137],"active":[138],"suitable":[140],"downstream":[142],"analyses":[143],"such":[144],"as":[145],"RNA":[146],"sequencing,":[147],"structural":[148],"analysis,":[149],"mass":[151],"spectrometry":[152],"peptide":[153],"identification.":[154]},"counts_by_year":[{"year":2014,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}