{"id":"https://openalex.org/W223847686","doi":"https://doi.org/10.1023/a:1024669810540","title":"Trp17 and Glu20 Residues in Conserved WMN(D/E)PN Motif Are Essential for Aspergillus ficuum Endoinulinase (EC 3.2.1.7) Activity","display_name":"Trp17 and Glu20 Residues in Conserved WMN(D/E)PN Motif Are Essential for Aspergillus ficuum Endoinulinase (EC 3.2.1.7) Activity","publication_year":2003,"publication_date":"2003-06-01","ids":{"openalex":"https://openalex.org/W223847686","doi":"https://doi.org/10.1023/a:1024669810540","mag":"223847686","pmid":"https://pubmed.ncbi.nlm.nih.gov/12943511"},"language":"en","primary_location":{"id":"doi:10.1023/a:1024669810540","doi":"https://doi.org/10.1023/a:1024669810540","is_oa":false,"landing_page_url":"https://doi.org/10.1023/a:1024669810540","pdf_url":null,"source":{"id":"https://openalex.org/S71215691","display_name":"Biochemistry (Moscow)","issn_l":"0006-2979","issn":["0006-2979","0320-9725","1608-3040"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320267","host_organization_name":"Pleiades Publishing","host_organization_lineage":["https://openalex.org/P4310320267","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Pleiades Publishing","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry (Moscow)","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5066472160","display_name":"Seockyu Park","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I80611190","display_name":"Jeonbuk National University","ror":"https://ror.org/05q92br09","country_code":"KR","type":"education","lineage":["https://openalex.org/I80611190"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Seockyu Park","raw_affiliation_strings":["Division of Biological Sciences and Research Center for Industrial Development of Biofood Materials, Chonbuk National University, Chonju, Chonbuk 561-756, Republic of Korea","Division of Biological Sciences and Research Center for Industrial Development of Biofood Materials, Chonbuk National University, Chonju, Chonbuk, Republic of Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Biological Sciences and Research Center for Industrial Development of Biofood Materials, Chonbuk National University, Chonju, Chonbuk 561-756, Republic of Korea","institution_ids":["https://openalex.org/I80611190"]},{"raw_affiliation_string":"Division of Biological Sciences and Research Center for Industrial Development of Biofood Materials, Chonbuk National University, Chonju, Chonbuk, Republic of Korea","institution_ids":["https://openalex.org/I80611190"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101082779","display_name":"Yeonsoo Han","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I80611190","display_name":"Jeonbuk National University","ror":"https://ror.org/05q92br09","country_code":"KR","type":"education","lineage":["https://openalex.org/I80611190"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Yeonsoo Han","raw_affiliation_strings":["Division of Biological Sciences and Research Center for Industrial Development of Biofood Materials, Chonbuk National University, Chonju, Chonbuk, 561-756, Republic of Korea","Division of Biological Sciences and Research Center for Industrial Development of Biofood Materials, Chonbuk National University, Chonju, Chonbuk, Republic of Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Biological Sciences and Research Center for Industrial Development of Biofood Materials, Chonbuk National University, Chonju, Chonbuk, 561-756, Republic of Korea","institution_ids":["https://openalex.org/I80611190"]},{"raw_affiliation_string":"Division of Biological Sciences and Research Center for Industrial Development of Biofood Materials, Chonbuk National University, Chonju, Chonbuk, Republic of Korea","institution_ids":["https://openalex.org/I80611190"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5007208136","display_name":"Heeun Kim","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I80611190","display_name":"Jeonbuk National University","ror":"https://ror.org/05q92br09","country_code":"KR","type":"education","lineage":["https://openalex.org/I80611190"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Heeun Kim","raw_affiliation_strings":["Division of Biological Sciences and Research Center for Industrial Development of Biofood Materials, Chonbuk National University, Chonju, Chonbuk, 561-756, Republic of Korea","Division of Biological Sciences and Research Center for Industrial Development of Biofood Materials, Chonbuk National University, Chonju, Chonbuk, Republic of Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Biological Sciences and Research Center for Industrial Development of Biofood Materials, Chonbuk National University, Chonju, Chonbuk, 561-756, Republic of Korea","institution_ids":["https://openalex.org/I80611190"]},{"raw_affiliation_string":"Division of Biological Sciences and Research Center for Industrial Development of Biofood Materials, Chonbuk National University, Chonju, Chonbuk, Republic of Korea","institution_ids":["https://openalex.org/I80611190"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5090549376","display_name":"Song-Yi Song","orcid":"https://orcid.org/0009-0004-0665-6219","observed_orcids":["https://orcid.org/0009-0004-0665-6219"]},"institutions":[{"id":"https://openalex.org/I80611190","display_name":"Jeonbuk National University","ror":"https://ror.org/05q92br09","country_code":"KR","type":"education","lineage":["https://openalex.org/I80611190"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Songyi Song","raw_affiliation_strings":["Division of Biological Sciences and Research Center for Industrial Development of Biofood Materials, Chonbuk National University, Chonju, Chonbuk, 561-756, Republic of Korea","Division of Biological Sciences and Research Center for Industrial Development of Biofood Materials, Chonbuk National University, Chonju, Chonbuk, Republic of Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Biological Sciences and Research Center for Industrial Development of Biofood Materials, Chonbuk National University, Chonju, Chonbuk, 561-756, Republic of Korea","institution_ids":["https://openalex.org/I80611190"]},{"raw_affiliation_string":"Division of Biological Sciences and Research Center for Industrial Development of Biofood Materials, Chonbuk National University, Chonju, Chonbuk, Republic of Korea","institution_ids":["https://openalex.org/I80611190"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038953973","display_name":"Tai-Boong Uhm","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I80611190","display_name":"Jeonbuk National University","ror":"https://ror.org/05q92br09","country_code":"KR","type":"education","lineage":["https://openalex.org/I80611190"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Tai-Boong Uhm","raw_affiliation_strings":["Division of Biological Sciences and Research Center for Industrial Development of Biofood Materials, Chonbuk National University, Chonju, Chonbuk, 561-756, Republic of Korea","Division of Biological Sciences and Research Center for Industrial Development of Biofood Materials, Chonbuk National University, Chonju, Chonbuk, Republic of Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Biological Sciences and Research Center for Industrial Development of Biofood Materials, Chonbuk National University, Chonju, Chonbuk, 561-756, Republic of Korea","institution_ids":["https://openalex.org/I80611190"]},{"raw_affiliation_string":"Division of Biological Sciences and Research Center for Industrial Development of Biofood Materials, Chonbuk National University, Chonju, Chonbuk, Republic of Korea","institution_ids":["https://openalex.org/I80611190"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5108543617","display_name":"Keon\u2010Sang Chae","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I80611190","display_name":"Jeonbuk National University","ror":"https://ror.org/05q92br09","country_code":"KR","type":"education","lineage":["https://openalex.org/I80611190"]}],"countries":["KR"],"is_corresponding":true,"raw_author_name":"Keon-Sang Chae","raw_affiliation_strings":["Division of Biological Sciences and Research Center for Industrial Development of Biofood Materials, Chonbuk National University, Chonju, Chonbuk, 561-756, Republic of Korea","Division of Biological Sciences and Research Center for Industrial Development of Biofood Materials, Chonbuk National University, Chonju, Chonbuk, Republic of Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Biological Sciences and Research Center for Industrial Development of Biofood Materials, Chonbuk National University, Chonju, Chonbuk, 561-756, Republic of Korea","institution_ids":["https://openalex.org/I80611190"]},{"raw_affiliation_string":"Division of Biological Sciences and Research Center for Industrial Development of Biofood Materials, Chonbuk National University, Chonju, Chonbuk, Republic of Korea","institution_ids":["https://openalex.org/I80611190"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5108543617"],"corresponding_institution_ids":["https://openalex.org/I80611190"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":7,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":95},"biblio":{"volume":"68","issue":"6","first_page":"658","last_page":"661"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12489","display_name":"Microbial Metabolites in Food Biotechnology","score":0.5145999789237976,"subfield":{"id":"https://openalex.org/subfields/2916","display_name":"Nutrition and Dietetics"},"field":{"id":"https://openalex.org/fields/29","display_name":"Nursing"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T12489","display_name":"Microbial Metabolites in Food Biotechnology","score":0.5145999789237976,"subfield":{"id":"https://openalex.org/subfields/2916","display_name":"Nutrition and Dietetics"},"field":{"id":"https://openalex.org/fields/29","display_name":"Nursing"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.37229999899864197,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.0494999997317791,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/endoinulinase","display_name":"endoinulinase","score":0.878000020980835},{"id":"https://openalex.org/keywords/aspergillus-ficuum","display_name":"Aspergillus ficuum","score":0.9900000095367432},{"id":"https://openalex.org/keywords/invertase","display_name":"invertase","score":0.9010000228881836},{"id":"https://openalex.org/keywords/site-directed-mutagenesis","display_name":"site-directed mutagenesis","score":0.9079999923706055},{"id":"https://openalex.org/keywords/enzyme-activity","display_name":"enzyme activity","score":0.8610000014305115}],"concepts":[{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.7926351428031921},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.635373592376709},{"id":"https://openalex.org/C32276052","wikidata":"https://www.wikidata.org/wiki/Q908349","display_name":"Motif (music)","level":2,"score":0.5612787008285522},{"id":"https://openalex.org/C199216141","wikidata":"https://www.wikidata.org/wiki/Q4995178","display_name":"Conserved sequence","level":4,"score":0.5375497341156006},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5232455134391785},{"id":"https://openalex.org/C207583985","wikidata":"https://www.wikidata.org/wiki/Q128723","display_name":"Wild type","level":4,"score":0.5088934898376465},{"id":"https://openalex.org/C103408237","wikidata":"https://www.wikidata.org/wiki/Q2642668","display_name":"Site-directed mutagenesis","level":4,"score":0.48284560441970825},{"id":"https://openalex.org/C28212737","wikidata":"https://www.wikidata.org/wiki/Q5381543","display_name":"Enzyme assay","level":3,"score":0.44322219491004944},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4425750970840454},{"id":"https://openalex.org/C117745874","wikidata":"https://www.wikidata.org/wiki/Q901612","display_name":"Sequence motif","level":3,"score":0.432317316532135},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.35926955938339233},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.34181827306747437},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.1964757740497589},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.168157696723938},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C24890656","wikidata":"https://www.wikidata.org/wiki/Q82811","display_name":"Acoustics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001230","descriptor_name":"Aspergillus","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D006026","descriptor_name":"Glycoside Hydrolases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D006026","descriptor_name":"Glycoside Hydrolases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014364","descriptor_name":"Tryptophan","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D016297","descriptor_name":"Mutagenesis, Site-Directed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017124","descriptor_name":"Conserved Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D018698","descriptor_name":"Glutamic Acid","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D019943","descriptor_name":"Amino Acid Substitution","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020816","descriptor_name":"Amino Acid Motifs","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1023/a:1024669810540","doi":"https://doi.org/10.1023/a:1024669810540","is_oa":false,"landing_page_url":"https://doi.org/10.1023/a:1024669810540","pdf_url":null,"source":{"id":"https://openalex.org/S71215691","display_name":"Biochemistry (Moscow)","issn_l":"0006-2979","issn":["0006-2979","0320-9725","1608-3040"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320267","host_organization_name":"Pleiades Publishing","host_organization_lineage":["https://openalex.org/P4310320267","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Pleiades Publishing","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry (Moscow)","raw_type":"journal-article"},{"id":"pmid:12943511","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12943511","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry. Biokhimiia","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":15,"referenced_works":["https://openalex.org/W55257032","https://openalex.org/W321641302","https://openalex.org/W1543832775","https://openalex.org/W1606988944","https://openalex.org/W1973782344","https://openalex.org/W1977906958","https://openalex.org/W1985257078","https://openalex.org/W1987250042","https://openalex.org/W1994311403","https://openalex.org/W1997395050","https://openalex.org/W2052389444","https://openalex.org/W2069824712","https://openalex.org/W2092118173","https://openalex.org/W2106121486","https://openalex.org/W2950469006"],"related_works":["https://openalex.org/W2075631102","https://openalex.org/W1986460389","https://openalex.org/W2378528012","https://openalex.org/W1990085947","https://openalex.org/W7039599843","https://openalex.org/W2046242540","https://openalex.org/W2085575539","https://openalex.org/W1988204388","https://openalex.org/W2330505095","https://openalex.org/W7033978708"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2016-06-24T00:00:00"}