{"id":"https://openalex.org/W2238018570","doi":"https://doi.org/10.1074/jbc.m115.673152","title":"Structure and Properties of a Non-processive, Salt-requiring, and Acidophilic Pectin Methylesterase from Aspergillus niger Provide Insights into the Key Determinants of Processivity Control","display_name":"Structure and Properties of a Non-processive, Salt-requiring, and Acidophilic Pectin Methylesterase from Aspergillus niger Provide Insights into the Key Determinants of Processivity Control","publication_year":2015,"publication_date":"2015-11-16","ids":{"openalex":"https://openalex.org/W2238018570","doi":"https://doi.org/10.1074/jbc.m115.673152","mag":"2238018570","pmid":"https://pubmed.ncbi.nlm.nih.gov/26567911","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/4714216"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.m115.673152","doi":"https://doi.org/10.1074/jbc.m115.673152","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m115.673152","pdf_url":"http://www.jbc.org/article/S0021925820361640/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"http://www.jbc.org/article/S0021925820361640/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5079787628","display_name":"Lisa M. Kent","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210159946","display_name":"Riddet Institute","ror":"https://ror.org/053336823","country_code":"NZ","type":"facility","lineage":["https://openalex.org/I1320288732","https://openalex.org/I154130895","https://openalex.org/I41125697","https://openalex.org/I4210159946","https://openalex.org/I4405260297","https://openalex.org/I51158804","https://openalex.org/I80281795"]},{"id":"https://openalex.org/I51158804","display_name":"Massey University","ror":"https://ror.org/052czxv31","country_code":"NZ","type":"education","lineage":["https://openalex.org/I51158804"]}],"countries":["NZ"],"is_corresponding":false,"raw_author_name":"Lisa M. Kent","raw_affiliation_strings":["From Riddet Institute and Institute of Fundamental Sciences, Massey University, Palmerston North 4442, New Zealand","From Riddet Institute and","Institute of Fundamental Sciences, Massey University, Palmerston North 4442, New Zealand,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From Riddet Institute and Institute of Fundamental Sciences, Massey University, Palmerston North 4442, New Zealand","institution_ids":["https://openalex.org/I4210159946","https://openalex.org/I51158804"]},{"raw_affiliation_string":"From Riddet Institute and","institution_ids":["https://openalex.org/I4210159946"]},{"raw_affiliation_string":"Institute of Fundamental Sciences, Massey University, Palmerston North 4442, New Zealand,","institution_ids":["https://openalex.org/I51158804"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5069764771","display_name":"Trevor S. Loo","orcid":"https://orcid.org/0000-0002-0501-5522","observed_orcids":["https://orcid.org/0000-0002-0501-5522"]},"institutions":[{"id":"https://openalex.org/I4210159946","display_name":"Riddet Institute","ror":"https://ror.org/053336823","country_code":"NZ","type":"facility","lineage":["https://openalex.org/I1320288732","https://openalex.org/I154130895","https://openalex.org/I41125697","https://openalex.org/I4210159946","https://openalex.org/I4405260297","https://openalex.org/I51158804","https://openalex.org/I80281795"]},{"id":"https://openalex.org/I51158804","display_name":"Massey University","ror":"https://ror.org/052czxv31","country_code":"NZ","type":"education","lineage":["https://openalex.org/I51158804"]}],"countries":["NZ"],"is_corresponding":false,"raw_author_name":"Trevor S. Loo","raw_affiliation_strings":["From Riddet Institute and Institute of Fundamental Sciences, Massey University, Palmerston North 4442, New Zealand","From Riddet Institute and","Institute of Fundamental Sciences, Massey University, Palmerston North 4442, New Zealand,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From Riddet Institute and Institute of Fundamental Sciences, Massey University, Palmerston North 4442, New Zealand","institution_ids":["https://openalex.org/I4210159946","https://openalex.org/I51158804"]},{"raw_affiliation_string":"From Riddet Institute and","institution_ids":["https://openalex.org/I4210159946"]},{"raw_affiliation_string":"Institute of Fundamental Sciences, Massey University, Palmerston North 4442, New Zealand,","institution_ids":["https://openalex.org/I51158804"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5084614375","display_name":"Laurence D. Melton","orcid":"https://orcid.org/0000-0002-0166-9414","observed_orcids":["https://orcid.org/0000-0002-0166-9414"]},"institutions":[{"id":"https://openalex.org/I154130895","display_name":"University of Auckland","ror":"https://ror.org/03b94tp07","country_code":"NZ","type":"education","lineage":["https://openalex.org/I154130895"]},{"id":"https://openalex.org/I4210159946","display_name":"Riddet Institute","ror":"https://ror.org/053336823","country_code":"NZ","type":"facility","lineage":["https://openalex.org/I1320288732","https://openalex.org/I154130895","https://openalex.org/I41125697","https://openalex.org/I4210159946","https://openalex.org/I4405260297","https://openalex.org/I51158804","https://openalex.org/I80281795"]}],"countries":["NZ"],"is_corresponding":false,"raw_author_name":"Laurence D. Melton","raw_affiliation_strings":["From Riddet Institute and School of Chemical Sciences, University of Auckland, Auckland 1142, New Zealand","From Riddet Institute and","School of Chemical Sciences, University of Auckland, Auckland 1142, New Zealand,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From Riddet Institute and School of Chemical Sciences, University of Auckland, Auckland 1142, New Zealand","institution_ids":["https://openalex.org/I154130895","https://openalex.org/I4210159946"]},{"raw_affiliation_string":"From Riddet Institute and","institution_ids":["https://openalex.org/I4210159946"]},{"raw_affiliation_string":"School of Chemical Sciences, University of Auckland, Auckland 1142, New Zealand,","institution_ids":["https://openalex.org/I154130895"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5029024911","display_name":"Davide Mercadante","orcid":"https://orcid.org/0000-0001-6792-7706","observed_orcids":["https://orcid.org/0000-0001-6792-7706"]},"institutions":[{"id":"https://openalex.org/I4210159946","display_name":"Riddet Institute","ror":"https://ror.org/053336823","country_code":"NZ","type":"facility","lineage":["https://openalex.org/I1320288732","https://openalex.org/I154130895","https://openalex.org/I41125697","https://openalex.org/I4210159946","https://openalex.org/I4405260297","https://openalex.org/I51158804","https://openalex.org/I80281795"]},{"id":"https://openalex.org/I71030271","display_name":"Heidelberg Institute for Theoretical Studies","ror":"https://ror.org/01f7bcy98","country_code":"DE","type":"facility","lineage":["https://openalex.org/I102335020","https://openalex.org/I1305996414","https://openalex.org/I223822909","https://openalex.org/I71030271"]}],"countries":["DE","NZ"],"is_corresponding":false,"raw_author_name":"Davide Mercadante","raw_affiliation_strings":["From Riddet Institute and Molecular Biomechanics Group, Heidelberg Institute for Theoretical Studies, Schloss-Wolfsbrunnenweg, 69118 Heidelberg, Germany, and","From Riddet Institute and","Molecular Biomechanics Group, Heidelberg Institute for Theoretical Studies, Schloss-Wolfsbrunnenweg, 69118 Heidelberg, Germany, and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From Riddet Institute and Molecular Biomechanics Group, Heidelberg Institute for Theoretical Studies, Schloss-Wolfsbrunnenweg, 69118 Heidelberg, Germany, and","institution_ids":["https://openalex.org/I71030271"]},{"raw_affiliation_string":"From Riddet Institute and","institution_ids":["https://openalex.org/I4210159946"]},{"raw_affiliation_string":"Molecular Biomechanics Group, Heidelberg Institute for Theoretical Studies, Schloss-Wolfsbrunnenweg, 69118 Heidelberg, Germany, and","institution_ids":["https://openalex.org/I71030271"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5055773574","display_name":"Martin A. K. Williams","orcid":"https://orcid.org/0000-0002-7707-4871","observed_orcids":["https://orcid.org/0000-0002-7707-4871"]},"institutions":[{"id":"https://openalex.org/I4210159946","display_name":"Riddet Institute","ror":"https://ror.org/053336823","country_code":"NZ","type":"facility","lineage":["https://openalex.org/I1320288732","https://openalex.org/I154130895","https://openalex.org/I41125697","https://openalex.org/I4210159946","https://openalex.org/I4405260297","https://openalex.org/I51158804","https://openalex.org/I80281795"]},{"id":"https://openalex.org/I51158804","display_name":"Massey University","ror":"https://ror.org/052czxv31","country_code":"NZ","type":"education","lineage":["https://openalex.org/I51158804"]},{"id":"https://openalex.org/I99121787","display_name":"MacDiarmid Institute for Advanced Materials and Nanotechnology","ror":"https://ror.org/04gjfdj81","country_code":"NZ","type":"facility","lineage":["https://openalex.org/I41156924","https://openalex.org/I99121787"]}],"countries":["NZ"],"is_corresponding":true,"raw_author_name":"Martin A.K. Williams","raw_affiliation_strings":["From Riddet Institute and Institute of Fundamental Sciences, Massey University, Palmerston North 4442, New Zealand, MacDiarmid Institute for Advanced Materials and Nanotechnology, Palmerston North 4442, New Zealand m.williams@massey.ac.nz","From Riddet Institute and","Institute of Fundamental Sciences, Massey University, Palmerston North 4442, New Zealand,","MacDiarmid Institute for Advanced Materials and Nanotechnology, Palmerston North 4442, New Zealand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From Riddet Institute and Institute of Fundamental Sciences, Massey University, Palmerston North 4442, New Zealand, MacDiarmid Institute for Advanced Materials and Nanotechnology, Palmerston North 4442, New Zealand m.williams@massey.ac.nz","institution_ids":["https://openalex.org/I4210159946","https://openalex.org/I51158804","https://openalex.org/I99121787"]},{"raw_affiliation_string":"From Riddet Institute and","institution_ids":["https://openalex.org/I4210159946"]},{"raw_affiliation_string":"Institute of Fundamental Sciences, Massey University, Palmerston North 4442, New Zealand,","institution_ids":["https://openalex.org/I51158804"]},{"raw_affiliation_string":"MacDiarmid Institute for Advanced Materials and Nanotechnology, Palmerston North 4442, New Zealand","institution_ids":["https://openalex.org/I99121787"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5028603943","display_name":"Geoffrey B. Jameson","orcid":"https://orcid.org/0000-0003-4839-0784","observed_orcids":["https://orcid.org/0000-0003-4839-0784"]},"institutions":[{"id":"https://openalex.org/I4210159946","display_name":"Riddet Institute","ror":"https://ror.org/053336823","country_code":"NZ","type":"facility","lineage":["https://openalex.org/I1320288732","https://openalex.org/I154130895","https://openalex.org/I41125697","https://openalex.org/I4210159946","https://openalex.org/I4405260297","https://openalex.org/I51158804","https://openalex.org/I80281795"]},{"id":"https://openalex.org/I51158804","display_name":"Massey University","ror":"https://ror.org/052czxv31","country_code":"NZ","type":"education","lineage":["https://openalex.org/I51158804"]},{"id":"https://openalex.org/I99121787","display_name":"MacDiarmid Institute for Advanced Materials and Nanotechnology","ror":"https://ror.org/04gjfdj81","country_code":"NZ","type":"facility","lineage":["https://openalex.org/I41156924","https://openalex.org/I99121787"]}],"countries":["NZ"],"is_corresponding":true,"raw_author_name":"Geoffrey B. Jameson","raw_affiliation_strings":["From Riddet Institute and Institute of Fundamental Sciences, Massey University, Palmerston North 4442, New Zealand, MacDiarmid Institute for Advanced Materials and Nanotechnology, Palmerston North 4442, New Zealand g.b.jameson@massey.ac.nz","From Riddet Institute and","Institute of Fundamental Sciences, Massey University, Palmerston North 4442, New Zealand,","MacDiarmid Institute for Advanced Materials and Nanotechnology, Palmerston North 4442, New Zealand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From Riddet Institute and Institute of Fundamental Sciences, Massey University, Palmerston North 4442, New Zealand, MacDiarmid Institute for Advanced Materials and Nanotechnology, Palmerston North 4442, New Zealand g.b.jameson@massey.ac.nz","institution_ids":["https://openalex.org/I4210159946","https://openalex.org/I51158804","https://openalex.org/I99121787"]},{"raw_affiliation_string":"From Riddet Institute and","institution_ids":["https://openalex.org/I4210159946"]},{"raw_affiliation_string":"Institute of Fundamental Sciences, Massey University, Palmerston North 4442, New Zealand,","institution_ids":["https://openalex.org/I51158804"]},{"raw_affiliation_string":"MacDiarmid Institute for Advanced Materials and Nanotechnology, Palmerston North 4442, New Zealand","institution_ids":["https://openalex.org/I99121787"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5028603943","https://openalex.org/A5055773574"],"corresponding_institution_ids":["https://openalex.org/I4210159946","https://openalex.org/I51158804","https://openalex.org/I99121787"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":3.395,"has_fulltext":true,"cited_by_count":41,"citation_normalized_percentile":{"value":0.91607004,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"291","issue":"3","first_page":"1289","last_page":"1306"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10869","display_name":"Polysaccharides and Plant Cell Walls","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10869","display_name":"Polysaccharides and Plant Cell Walls","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/pectin-methylesterase","display_name":"pectin methylesterase","score":0.9980000257492065},{"id":"https://openalex.org/keywords/aspergillus-niger","display_name":"Aspergillus niger","score":0.9819999933242798},{"id":"https://openalex.org/keywords/homogalacturonan","display_name":"homogalacturonan","score":0.7369999885559082},{"id":"https://openalex.org/keywords/pectin-demethylesterification","display_name":"pectin demethylesterification","score":0.8600000143051147},{"id":"https://openalex.org/keywords/salt-requirement","display_name":"salt requirement","score":0.4970000088214874},{"id":"https://openalex.org/keywords/acidophilic-enzymes","display_name":"acidophilic enzymes","score":0.8610000014305115},{"id":"https://openalex.org/keywords/electrostatic-potential","display_name":"electrostatic potential","score":0.7269999980926514},{"id":"https://openalex.org/keywords/substrate-binding-groove","display_name":"substrate-binding groove","score":0.703000009059906},{"id":"https://openalex.org/keywords/glycosidic-bond-rotation","display_name":"glycosidic bond rotation","score":0.8849999904632568},{"id":"https://openalex.org/keywords/x-ray-crystal-structure","display_name":"X-ray crystal structure","score":0.5609999895095825},{"id":"https://openalex.org/keywords/molecular-dynamics-simulation","display_name":"molecular dynamics simulation","score":0.6909999847412109}],"concepts":[{"id":"https://openalex.org/C2775960407","wikidata":"https://www.wikidata.org/wiki/Q132014","display_name":"Aspergillus niger","level":2,"score":0.8739439845085144},{"id":"https://openalex.org/C131140672","wikidata":"https://www.wikidata.org/wiki/Q2031164","display_name":"Processivity","level":4,"score":0.8370422720909119},{"id":"https://openalex.org/C2780756937","wikidata":"https://www.wikidata.org/wiki/Q188154","display_name":"Pectin","level":2,"score":0.6149821877479553},{"id":"https://openalex.org/C26517878","wikidata":"https://www.wikidata.org/wiki/Q228039","display_name":"Key (lock)","level":2,"score":0.5226618647575378},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.46342313289642334},{"id":"https://openalex.org/C2779175654","wikidata":"https://www.wikidata.org/wiki/Q335130","display_name":"Aspergillus","level":2,"score":0.4296703040599823},{"id":"https://openalex.org/C2776371256","wikidata":"https://www.wikidata.org/wiki/Q12370","display_name":"Salt (chemistry)","level":2,"score":0.42916417121887207},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3634546995162964},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.32203859090805054},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2899837791919708},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2847704291343689},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.12741631269454956},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.07794246077537537},{"id":"https://openalex.org/C82381507","wikidata":"https://www.wikidata.org/wiki/Q416878","display_name":"Polymerase","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001234","descriptor_name":"Aspergillus niger","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D002265","descriptor_name":"Carboxylic Ester Hydrolases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D002265","descriptor_name":"Carboxylic Ester Hydrolases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002265","descriptor_name":"Carboxylic Ester Hydrolases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D005656","descriptor_name":"Fungal Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005656","descriptor_name":"Fungal Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005656","descriptor_name":"Fungal Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009994","descriptor_name":"Osmolar Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010446","descriptor_name":"Peptide Fragments","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010446","descriptor_name":"Peptide Fragments","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010446","descriptor_name":"Peptide Fragments","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014961","descriptor_name":"X-Ray Diffraction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016384","descriptor_name":"Consensus Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016415","descriptor_name":"Sequence Alignment","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019943","descriptor_name":"Amino Acid Substitution","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020134","descriptor_name":"Catalytic Domain","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D040681","descriptor_name":"Structural Homology, Protein","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D056004","descriptor_name":"Molecular Dynamics Simulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1074/jbc.m115.673152","doi":"https://doi.org/10.1074/jbc.m115.673152","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m115.673152","pdf_url":"http://www.jbc.org/article/S0021925820361640/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:4127308","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4714216","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:4714216","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4714216","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Biol Chem","raw_type":"Text"},{"id":"pmid:26567911","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/26567911","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Comparative Study"}],"best_oa_location":{"id":"doi:10.1074/jbc.m115.673152","doi":"https://doi.org/10.1074/jbc.m115.673152","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m115.673152","pdf_url":"http://www.jbc.org/article/S0021925820361640/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320330278","display_name":"Riddet Institute","ror":"https://ror.org/053336823"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2238018570.pdf","grobid_xml":"https://content.openalex.org/works/W2238018570.grobid-xml"},"referenced_works_count":100,"referenced_works":["https://openalex.org/W1487019814","https://openalex.org/W1515226261","https://openalex.org/W1535444533","https://openalex.org/W1575265895","https://openalex.org/W1615351854","https://openalex.org/W1890950143","https://openalex.org/W1967632262","https://openalex.org/W1969547178","https://openalex.org/W1973599327","https://openalex.org/W1975078013","https://openalex.org/W1975856799","https://openalex.org/W1975976514","https://openalex.org/W1980286892","https://openalex.org/W1989646149","https://openalex.org/W1989776372","https://openalex.org/W1991366673","https://openalex.org/W1996319727","https://openalex.org/W1998713720","https://openalex.org/W1998783353","https://openalex.org/W1998884733","https://openalex.org/W1999577767","https://openalex.org/W2001571163","https://openalex.org/W2001641653","https://openalex.org/W2004580504","https://openalex.org/W2006260586","https://openalex.org/W2007306716","https://openalex.org/W2013550509","https://openalex.org/W2014682260","https://openalex.org/W2016446798","https://openalex.org/W2016740325","https://openalex.org/W2016997088","https://openalex.org/W2020398662","https://openalex.org/W2024937427","https://openalex.org/W2025002672","https://openalex.org/W2026993811","https://openalex.org/W2028646481","https://openalex.org/W2033766616","https://openalex.org/W2034996163","https://openalex.org/W2038184529","https://openalex.org/W2038747144","https://openalex.org/W2040474140","https://openalex.org/W2041334380","https://openalex.org/W2043827982","https://openalex.org/W2043844194","https://openalex.org/W2045078163","https://openalex.org/W2046241405","https://openalex.org/W2048803160","https://openalex.org/W2050093771","https://openalex.org/W2051558036","https://openalex.org/W2059277134","https://openalex.org/W2061926198","https://openalex.org/W2063178767","https://openalex.org/W2063916577","https://openalex.org/W2065922524","https://openalex.org/W2072475028","https://openalex.org/W2074509994","https://openalex.org/W2075369230","https://openalex.org/W2076046125","https://openalex.org/W2077697000","https://openalex.org/W2084858774","https://openalex.org/W2087589668","https://openalex.org/W2088313631","https://openalex.org/W2091468419","https://openalex.org/W2094919538","https://openalex.org/W2095231689","https://openalex.org/W2095632226","https://openalex.org/W2097182393","https://openalex.org/W2097546452","https://openalex.org/W2097573838","https://openalex.org/W2106728647","https://openalex.org/W2106882534","https://openalex.org/W2108921801","https://openalex.org/W2109612279","https://openalex.org/W2116947993","https://openalex.org/W2120093636","https://openalex.org/W2124716473","https://openalex.org/W2133070242","https://openalex.org/W2133534930","https://openalex.org/W2135862107","https://openalex.org/W2137043026","https://openalex.org/W2140483228","https://openalex.org/W2144081223","https://openalex.org/W2144998676","https://openalex.org/W2146080841","https://openalex.org/W2148086604","https://openalex.org/W2157199045","https://openalex.org/W2158504652","https://openalex.org/W2158794108","https://openalex.org/W2159211495","https://openalex.org/W2163341755","https://openalex.org/W2163450836","https://openalex.org/W2166578555","https://openalex.org/W2313274246","https://openalex.org/W2315051068","https://openalex.org/W2330799739","https://openalex.org/W2473717171","https://openalex.org/W2786774014","https://openalex.org/W2950658938","https://openalex.org/W4285719527","https://openalex.org/W6720746794"],"related_works":["https://openalex.org/W2046241405","https://openalex.org/W2140483228","https://openalex.org/W4293171725","https://openalex.org/W2249996269","https://openalex.org/W2097182393","https://openalex.org/W2043827982","https://openalex.org/W3134050158","https://openalex.org/W2026993811","https://openalex.org/W4308327201","https://openalex.org/W7218196286"],"abstract_inverted_index":{"Many":[0],"pectin":[1],"methylesterases":[2],"(PMEs)":[3],"are":[4,19,129],"expressed":[5],"in":[6,74,142],"plants":[7],"to":[8,86,145],"modify":[9],"plant":[10],"cell-wall":[11],"pectins":[12,18],"for":[13],"various":[14],"physiological":[15],"roles.":[16],"These":[17],"also":[20],"attacked":[21],"by":[22,31],"PMEs":[23,32],"from":[24,71],"phytopathogens":[25],"and":[26,76,95,107,112,120,152],"phytophagous":[27],"insects.":[28],"The":[29,63],"de-methylesterification":[30,57],"of":[33,38,42,68,135,149],"the":[34,39,132,146,153],"O6-methyl":[35],"ester":[36],"groups":[37],"homogalacturonan":[40],"component":[41],"pectin,":[43],"exposing":[44],"galacturonic":[45],"acids,":[46],"can":[47],"occur":[48],"processively":[49],"or":[50],"non-processively,":[51],"respectively,":[52],"describing":[53],"sequential":[54],"versus":[55],"single":[56],"events":[58],"occurring":[59],"before":[60],"enzyme-substrate":[61],"dissociation.":[62],"high":[64],"resolution":[65],"x-ray":[66],"structures":[67],"a":[69,81,136],"PME":[70,103],"Aspergillus":[72],"niger":[73],"deglycosylated":[75],"Asn-linked":[77],"N-acetylglucosamine-stub":[78],"forms":[79],"reveal":[80,116],"10\u2154-turn":[82],"parallel":[83],"\u03b2-helix":[84],"(similar":[85],"but":[87],"with":[88,123],"less":[89],"extensive":[90],"loops":[91],"than":[92],"bacterial,":[93],"plant,":[94],"insect":[96],"PMEs).":[97],"Capillary":[98],"electrophoresis":[99],"shows":[100],"that":[101],"this":[102],"is":[104,156],"non-processive,":[105],"halophilic,":[106],"acidophilic.":[108],"Molecular":[109],"dynamics":[110],"simulations":[111],"electrostatic":[113],"potential":[114],"calculations":[115],"very":[117],"different":[118],"behavior":[119],"properties":[121],"compared":[122],"processive":[124,150],"PMEs.":[125],"Specifically,":[126],"uncorrelated":[127],"rotations":[128,148],"observed":[130],"about":[131],"glycosidic":[133],"bonds":[134],"partially":[137],"de-methyl-esterified":[138],"decasaccharide":[139],"model":[140],"substrate,":[141],"sharp":[143],"contrast":[144],"correlated":[147],"PMEs,":[151],"substrate-binding":[154],"groove":[155],"negatively":[157],"not":[158],"positively":[159],"charged.":[160]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":9},{"year":2022,"cited_by_count":6},{"year":2021,"cited_by_count":4},{"year":2020,"cited_by_count":4},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":6}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}