{"id":"https://openalex.org/W2235952887","doi":null,"title":"Carbohydrate-Interacting Proteins from Two Nostoc (Cyanobacteria) Species","display_name":"Carbohydrate-Interacting Proteins from Two Nostoc (Cyanobacteria) Species","publication_year":2004,"publication_date":"2004-02-18","ids":{"openalex":"https://openalex.org/W2235952887","mag":"2235952887"},"language":"en","primary_location":{"id":"pmh:oai:vtechworks.lib.vt.edu:10919/11177","doi":null,"is_oa":true,"landing_page_url":"http://scholar.lib.vt.edu/theses/available/etd-04242004-203124","pdf_url":"http://hdl.handle.net/10919/11177","source":{"id":"https://openalex.org/S4306400248","display_name":"VTechWorks (Virginia Tech)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I859038795","host_organization_name":"Virginia Tech","host_organization_lineage":["https://openalex.org/I859038795"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Dissertation"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://hdl.handle.net/10919/11177","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5083909930","display_name":"Brian R. Jordan","orcid":"https://orcid.org/0000-0003-0280-681X","observed_orcids":["https://orcid.org/0000-0003-0280-681X"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Brian R. Jordan","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5083909930"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":2,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12192","display_name":"Polysaccharides Composition and Applications","score":0.43309998512268066,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12192","display_name":"Polysaccharides Composition and Applications","score":0.43309998512268066,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.3831000030040741,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10869","display_name":"Polysaccharides and Plant Cell Walls","score":0.08749999850988388,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/glycosyltransferases","display_name":"glycosyltransferases","score":0.8740000128746033},{"id":"https://openalex.org/keywords/exopolysaccharides","display_name":"exopolysaccharides","score":0.9079999923706055},{"id":"https://openalex.org/keywords/anabaena-sp-pcc-7120","display_name":"Anabaena sp. PCC 7120","score":0.9470000267028809},{"id":"https://openalex.org/keywords/glucuronosyltransferase","display_name":"glucuronosyltransferase","score":0.9570000171661377},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.8109999895095825},{"id":"https://openalex.org/keywords/protein-aggregation","display_name":"protein aggregation","score":0.5720000267028809},{"id":"https://openalex.org/keywords/glycosylated-proteins","display_name":"glycosylated proteins","score":0.7950000166893005}],"concepts":[{"id":"https://openalex.org/C2779669040","wikidata":"https://www.wikidata.org/wiki/Q93315","display_name":"Cyanobacteria","level":3,"score":0.8706761002540588},{"id":"https://openalex.org/C2779465136","wikidata":"https://www.wikidata.org/wiki/Q311257","display_name":"Nostoc","level":4,"score":0.7650078535079956},{"id":"https://openalex.org/C2778977261","wikidata":"https://www.wikidata.org/wiki/Q11358","display_name":"Carbohydrate","level":2,"score":0.5293508172035217},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.42903733253479004},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3986791670322418},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3813280165195465},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.35513365268707275},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3506172299385071},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.33367788791656494},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.25817781686782837},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.14594808220863342}],"mesh":[],"locations_count":3,"locations":[{"id":"pmh:oai:vtechworks.lib.vt.edu:10919/11177","doi":null,"is_oa":true,"landing_page_url":"http://scholar.lib.vt.edu/theses/available/etd-04242004-203124","pdf_url":"http://hdl.handle.net/10919/11177","source":{"id":"https://openalex.org/S4306400248","display_name":"VTechWorks (Virginia Tech)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I859038795","host_organization_name":"Virginia Tech","host_organization_lineage":["https://openalex.org/I859038795"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Dissertation"},{"id":"pmh:oai:vtechworks.lib.vt.edu:10919/27206","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10919/27206","pdf_url":null,"source":{"id":"https://openalex.org/S4306400248","display_name":"VTechWorks (Virginia Tech)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I859038795","host_organization_name":"Virginia Tech","host_organization_lineage":["https://openalex.org/I859038795"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Dissertation"},{"id":"mag:2235952887","doi":null,"is_oa":false,"landing_page_url":"https://vtechworks.lib.vt.edu/bitstream/10919/27206/1/BrianJordanDissertation052004.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:vtechworks.lib.vt.edu:10919/11177","doi":null,"is_oa":true,"landing_page_url":"http://scholar.lib.vt.edu/theses/available/etd-04242004-203124","pdf_url":"http://hdl.handle.net/10919/11177","source":{"id":"https://openalex.org/S4306400248","display_name":"VTechWorks (Virginia Tech)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I859038795","host_organization_name":"Virginia Tech","host_organization_lineage":["https://openalex.org/I859038795"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Dissertation"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G8228315037","display_name":"BIOMIMETIC STRATEGIES FOR DESICCATION TOLERANT HUMAN CELLS","funder_award_id":"N00173021G016","funder_id":"https://openalex.org/F4320306078","funder_display_name":"U.S. Department of Defense"}],"funders":[{"id":"https://openalex.org/F4320306078","display_name":"U.S. Department of Defense","ror":"https://ror.org/0447fe631"},{"id":"https://openalex.org/F4320332180","display_name":"Defense Advanced Research Projects Agency","ror":"https://ror.org/02caytj08"},{"id":"https://openalex.org/F4320333591","display_name":"Multidisciplinary University Research Initiative","ror":null}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2235952887.pdf","grobid_xml":"https://content.openalex.org/works/W2235952887.grobid-xml"},"referenced_works_count":188,"referenced_works":["https://openalex.org/W187263312","https://openalex.org/W188565921","https://openalex.org/W194655167","https://openalex.org/W562908385","https://openalex.org/W1486602240","https://openalex.org/W1490286156","https://openalex.org/W1500699065","https://openalex.org/W1502542469","https://openalex.org/W1507280750","https://openalex.org/W1507396609","https://openalex.org/W1510403735","https://openalex.org/W1520546125","https://openalex.org/W1521060160","https://openalex.org/W1527158114","https://openalex.org/W1533225291","https://openalex.org/W1534791327","https://openalex.org/W1565706653","https://openalex.org/W1583532427","https://openalex.org/W1605513519","https://openalex.org/W1625885638","https://openalex.org/W1626710467","https://openalex.org/W1629931862","https://openalex.org/W1698181273","https://openalex.org/W1748166210","https://openalex.org/W1751818612","https://openalex.org/W1753402475","https://openalex.org/W1821152284","https://openalex.org/W1862479421","https://openalex.org/W1912870130","https://openalex.org/W1917697267","https://openalex.org/W1923584206","https://openalex.org/W1927693978","https://openalex.org/W1931878801","https://openalex.org/W1963733924","https://openalex.org/W1964660238","https://openalex.org/W1965396828","https://openalex.org/W1965569919","https://openalex.org/W1972615373","https://openalex.org/W1972936923","https://openalex.org/W1975085160","https://openalex.org/W1975288774","https://openalex.org/W1975553694","https://openalex.org/W1978223977","https://openalex.org/W1978722048","https://openalex.org/W1978833558","https://openalex.org/W1980587669","https://openalex.org/W1982011488","https://openalex.org/W1984371592","https://openalex.org/W1984492870","https://openalex.org/W1985771129","https://openalex.org/W1986573797","https://openalex.org/W1988066164","https://openalex.org/W1988396523","https://openalex.org/W1989039684","https://openalex.org/W1991745707","https://openalex.org/W1991787417","https://openalex.org/W1992302912","https://openalex.org/W1993904017","https://openalex.org/W1996064356","https://openalex.org/W1996161348","https://openalex.org/W1997031177","https://openalex.org/W2001282030","https://openalex.org/W2002613342","https://openalex.org/W2003046928","https://openalex.org/W2003117013","https://openalex.org/W2004704197","https://openalex.org/W2004710177","https://openalex.org/W2006077222","https://openalex.org/W2006385716","https://openalex.org/W2006517647","https://openalex.org/W2008245151","https://openalex.org/W2009665244","https://openalex.org/W2011548566","https://openalex.org/W2013419100","https://openalex.org/W2014798355","https://openalex.org/W2014899228","https://openalex.org/W2015292449","https://openalex.org/W2016934035","https://openalex.org/W2016982968","https://openalex.org/W2021398206","https://openalex.org/W2023585807","https://openalex.org/W2025476446","https://openalex.org/W2026024252","https://openalex.org/W2029388219","https://openalex.org/W2029880440","https://openalex.org/W2034535697","https://openalex.org/W2034671083","https://openalex.org/W2036962554","https://openalex.org/W2039568284","https://openalex.org/W2040307553","https://openalex.org/W2045474535","https://openalex.org/W2046746100","https://openalex.org/W2046920238","https://openalex.org/W2048791854","https://openalex.org/W2049443950","https://openalex.org/W2053361853","https://openalex.org/W2053828094","https://openalex.org/W2055553028","https://openalex.org/W2056031907","https://openalex.org/W2056920884","https://openalex.org/W2059144219","https://openalex.org/W2059421871","https://openalex.org/W2060061792","https://openalex.org/W2062806523","https://openalex.org/W2063031901","https://openalex.org/W2065098884","https://openalex.org/W2065815134","https://openalex.org/W2066933820","https://openalex.org/W2067212685","https://openalex.org/W2071221371","https://openalex.org/W2073359632","https://openalex.org/W2074953125","https://openalex.org/W2075307062","https://openalex.org/W2075459790","https://openalex.org/W2076686280","https://openalex.org/W2076777830","https://openalex.org/W2077277234","https://openalex.org/W2078702522","https://openalex.org/W2079754474","https://openalex.org/W2080791625","https://openalex.org/W2086418243","https://openalex.org/W2086420805","https://openalex.org/W2088349791","https://openalex.org/W2090014602","https://openalex.org/W2090677273","https://openalex.org/W2092962465","https://openalex.org/W2093198405","https://openalex.org/W2094696847","https://openalex.org/W2095347983","https://openalex.org/W2095418991","https://openalex.org/W2097648928","https://openalex.org/W2098255923","https://openalex.org/W2099553504","https://openalex.org/W2100532164","https://openalex.org/W2100837269","https://openalex.org/W2102114031","https://openalex.org/W2108216962","https://openalex.org/W2108605550","https://openalex.org/W2110103971","https://openalex.org/W2111006730","https://openalex.org/W2111535806","https://openalex.org/W2112768570","https://openalex.org/W2113151858","https://openalex.org/W2120537063","https://openalex.org/W2120701908","https://openalex.org/W2122400094","https://openalex.org/W2125775385","https://openalex.org/W2125939597","https://openalex.org/W2126384298","https://openalex.org/W2127881473","https://openalex.org/W2128433990","https://openalex.org/W2128635872","https://openalex.org/W2130381045","https://openalex.org/W2131686874","https://openalex.org/W2134419144","https://openalex.org/W2134822287","https://openalex.org/W2137581119","https://openalex.org/W2139246769","https://openalex.org/W2140094049","https://openalex.org/W2141708266","https://openalex.org/W2142879174","https://openalex.org/W2145008115","https://openalex.org/W2145301542","https://openalex.org/W2145458242","https://openalex.org/W2148192628","https://openalex.org/W2149320920","https://openalex.org/W2151020563","https://openalex.org/W2151726269","https://openalex.org/W2152951122","https://openalex.org/W2154393572","https://openalex.org/W2157120222","https://openalex.org/W2157563221","https://openalex.org/W2158714788","https://openalex.org/W2160493125","https://openalex.org/W2161530105","https://openalex.org/W2163006245","https://openalex.org/W2163798300","https://openalex.org/W2165256578","https://openalex.org/W2165750119","https://openalex.org/W2168015147","https://openalex.org/W2168421121","https://openalex.org/W2169696545","https://openalex.org/W2170894434","https://openalex.org/W2237782819","https://openalex.org/W2953019130","https://openalex.org/W2988949874","https://openalex.org/W3021509721","https://openalex.org/W4388189296"],"related_works":["https://openalex.org/W1968907063","https://openalex.org/W1986275321","https://openalex.org/W2274229267","https://openalex.org/W1979623447","https://openalex.org/W3207647685","https://openalex.org/W3090037590","https://openalex.org/W2621096870","https://openalex.org/W2151096361","https://openalex.org/W2084133812","https://openalex.org/W2022484626"],"abstract_inverted_index":{"Cyanobacteria":[0],"of":[1,21,27,32,38,62,71,128,241,247,273,279,297,308,346,383,387,416,421,428,464,480,487,531],"the":[2,8,33,50,58,69,136,143,165,214,274,277,290,305,336,380,393,413,422,429,454,459,478,503,513,517,525],"Nostoc":[3,34,63,99,111,178],"genus":[4],"are":[5,24,54,539],"known":[6],"for":[7,56,82,95,139],"thick,":[9],"mucilaginous":[10],"carbohydrate":[11,536],"coatings":[12],"that":[13,53,125,213,289,300,335,356,376,409,438,502,550],"they":[14],"produce.":[15],"In":[16],"this":[17,39,72,140,242,280,352,532],"work,":[18],"two":[19,441],"examples":[20],"cyanobacterial":[22,348,542],"glycobiology":[23],"considered,":[25],"each":[26],"which":[28,90,224,287],"involves":[29],"a":[30,85,119,157,284,310,344,358,362,369,373,407,417,488,553],"cyanobacterium":[31],"genus.\\nThe":[35],"first":[36],"portion":[37,240],"work":[40,243],"details":[41],"attempts":[42],"to":[43,67,74,93,145,163,228,321,325,343,379,467,475],"obtain":[44],"amino":[45,506],"acid":[46,507],"sequence":[47,332],"information":[48],"from":[49,132,177,303,412],"enzymes":[51,130],"(glycosyltransferases)":[52],"responsible":[55,138],"producing":[57],"extracellular":[59],"polysaccharide":[60],"(EPS)":[61],"commune":[64,100,112,179],"DRH1,":[65],"ultimately":[66],"allow":[68],"transfer":[70],"capacity":[73],"another":[75],"organism.":[76],"Two":[77],"artificial":[78],"substrates":[79],"were":[80,161,205,225,301,390,450],"synthesized":[81],"use":[83],"in":[84,98,108,207,351,392,477,512,541],"capillary":[86,152],"electrophoresis-based":[87,153],"enzyme":[88,117,137,154,167,174,216],"assay,":[89],"was":[91,106,150,175,196,217,404,410,462,522],"used":[92],"look":[94],"glycosyltransferase":[96,129],"activity":[97,105,141],"DRH1":[101],"cell":[102,254,424,545,556],"extracts.":[103],"Glucuronosyltransferase":[104],"detected":[107,337,394,468],"association":[109],"with":[110,329,453,524],"membrane":[113,180],"material.":[114,456,527],"The":[115,172,192],"active":[116,173,193,215],"displayed":[118],"divalent":[120],"cation":[121],"metal":[122],"dependence":[123],"(Mg+2)":[124],"is":[126,377],"typical":[127],"purified":[131,187],"other":[133,201],"organisms.":[134],"Because":[135],"held":[142],"potential":[144],"be":[146,322],"EPS-related,":[147],"its":[148],"purification":[149,170,202],"attempted.\\nThe":[151],"assay":[155],"and":[156,185,199,232,267,372,434,448,472,492,498,510],"32P-labeled":[158],"affinity":[159],"tag":[160],"utilized":[162],"follow":[164],"glucuronosyltransferase":[166],"through":[168],"successive":[169],"steps.":[171],"extracted":[176],"material":[181,461,533],"using":[182],"Triton":[183],"X-100,":[184],"then":[186,493],"by":[188,235,258,368,432,446,469,496,516],"anion":[189],"exchange":[190],"chromatography.":[191],"detergent":[194],"extract":[195,291],"extremely":[197],"unstable,":[198],"consequently,":[200],"techniques":[203],"tested":[204],"unsuccessful":[206],"enriching":[208],"activity.":[209],"Affinity-labeling":[210],"experiments":[211],"indicated":[212,334,437],"forming":[218],"protein":[219,331,483,514],"aggregates":[220],"during":[221],"these":[222,388],"procedures,":[223],"not":[226],"amenable":[227],"in-gel":[229],"protease":[230],"digestion":[231,486],"peptide":[233],"analysis":[234,495],"tandem":[236],"mass":[237,435,499],"spectrometry.\\nThe":[238],"second":[239],"describes":[244],"an":[245,248,481],"investigation":[246],"Anabaena":[249,395],"(Nostoc)":[250,396],"PCC":[251,397],"7120":[252,398],"soluble":[253,423],"extract.":[255,425],"Upon":[256],"separation":[257],"sodium":[259],"dodecyl":[260],"sulfate":[261],"\u00a1V":[262],"polyacrylamide":[263],"gel":[264,311,420,470],"electrophoresis":[265],"(SDS-PAGE)":[266],"subsequent":[268,494],"periodic":[269],"acid-Schiff":[270],"(PAS)":[271],"staining":[272],"resulting":[275,430],"gel,":[276],"components":[278],"cellular":[281],"fraction":[282],"produce":[283],"ladder-like":[285],"pattern,":[286],"suggests":[288],"may":[292],"contain":[293],"glycosylated":[294],"protein.":[295],"Analyses":[296],"several":[298],"samples":[299],"taken":[302],"within":[304],"PAS-staining":[306,414,455,460,526],"region":[307,375,382,415,504],"such":[309],"revealed":[312,501,534],"surface":[313],"layer":[314],"homology":[315],"(SLH)":[316],"domain-containing":[317,400,443],"proteins,":[318,444],"likely":[319],"candidates":[320],"covalently":[323],"attached":[324],"or":[326],"non-covalently":[327,451],"interacting":[328,452,482,523],"carbohydrate.\\nVarious":[330],"analyses":[333,427,530],"SLH":[338,364,399,442],"domain":[339,365],"containing":[340],"proteins":[341],"belong":[342],"family":[345,353],"(putative)":[347],"porins.":[349,385],"Proteins":[350],"possess":[354],"features":[355,389],"include":[357],"N-terminal":[359],"signal":[360],"sequence,":[361],"single":[363],"motif,":[366],"followed":[367],"coiled-coil":[370],"region,":[371],"C-terminal":[374],"homologous":[378],"b-barrel-forming":[381],"bacterial":[384],"All":[386],"identified":[391],"proteins.":[401],"Smith":[402],"degradation":[403],"performed":[405],"on":[406],"sample":[408,431],"electroeluted":[411],"preparative-scale":[418],"SDS-PAGE":[419,433,497],"Subsequent":[426],"spectrometry":[436],"at":[439],"least":[440],"encoded":[445,515],"all4499":[447],"alr4550,":[449],"Following":[457],"degradation,":[458],"still":[463],"sufficient":[465],"size":[466],"electrophoresis,":[471],"it":[473,551],"continued":[474],"migrate":[476],"absence":[479],"component.":[484],"Protease":[485],"similarly":[489],"prepared":[490],"sample,":[491],"spectrometry,":[500],"between":[505],"residues":[508],"#504":[509],"#536,":[511],"alr4550":[518],"open":[519],"reading":[520],"frame,":[521],"Monosaccharide":[528],"composition":[529],"more":[535],"constituents":[537],"than":[538],"found":[540],"primary":[543],"(peptidoglycan)":[544],"wall":[546,557],"polymer":[547,558],"alone,":[548],"indicating":[549],"contained":[552],"significant":[554],"secondary":[555],"component":[559],"as":[560],"well.":[561]},"counts_by_year":[{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
