{"id":"https://openalex.org/W2231034422","doi":null,"title":"Identification of the prion protein Doppel in spermatozoa and its impact in fertility","display_name":"Identification of the prion protein Doppel in spermatozoa and its impact in fertility","publication_year":2014,"publication_date":"2014-01-01","ids":{"openalex":"https://openalex.org/W2231034422","mag":"2231034422"},"language":"en","primary_location":{"id":"pmh:oai:repositorio.ulisboa.pt:10451/11853","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10451/11853","pdf_url":null,"source":{"id":"https://openalex.org/S4306402433","display_name":"Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"master thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://hdl.handle.net/10451/11853","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5046187379","display_name":"Leonor Matias Ferreira","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Leonor Matias Ferreira","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5046187379"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11335","display_name":"Prion Diseases and Protein Misfolding","score":0.6333000063896179,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11335","display_name":"Prion Diseases and Protein Misfolding","score":0.6333000063896179,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.3481000065803528,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12929","display_name":"Animal Genetics and Reproduction","score":0.00989999994635582,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/doppel-protein","display_name":"doppel protein","score":0.824999988079071},{"id":"https://openalex.org/keywords/prion-protein","display_name":"prion protein","score":0.7300000190734863},{"id":"https://openalex.org/keywords/spermatozoa","display_name":"spermatozoa","score":0.9020000100135803},{"id":"https://openalex.org/keywords/male-fertility","display_name":"male fertility","score":0.9929999709129333},{"id":"https://openalex.org/keywords/male-sterility","display_name":"male sterility","score":0.6470000147819519},{"id":"https://openalex.org/keywords/gametogenesis","display_name":"gametogenesis","score":0.6119999885559082},{"id":"https://openalex.org/keywords/sperm-capacitation","display_name":"sperm capacitation","score":0.6890000104904175},{"id":"https://openalex.org/keywords/fertilisation-biology","display_name":"fertilisation (biology)","score":0.6909999847412109},{"id":"https://openalex.org/keywords/zona-pellucida-penetration","display_name":"zona pellucida penetration","score":0.6930000185966492},{"id":"https://openalex.org/keywords/transposable-elements","display_name":"transposable elements","score":0.5659999847412109},{"id":"https://openalex.org/keywords/transposon-silencing","display_name":"transposon silencing","score":0.492000013589859},{"id":"https://openalex.org/keywords/transposable-element-mobilization","display_name":"transposable element mobilization","score":0.5429999828338623},{"id":"https://openalex.org/keywords/transposable-element-insertion","display_name":"transposable element insertion","score":0.37599998712539673},{"id":"https://openalex.org/keywords/transposable-element-copy-number","display_name":"transposable element copy number","score":0.4350000023841858}],"concepts":[{"id":"https://openalex.org/C518429986","wikidata":"https://www.wikidata.org/wiki/Q964401","display_name":"Fertility","level":3,"score":0.7166874408721924},{"id":"https://openalex.org/C116834253","wikidata":"https://www.wikidata.org/wiki/Q2039217","display_name":"Identification (biology)","level":2,"score":0.694502592086792},{"id":"https://openalex.org/C3019804061","wikidata":"https://www.wikidata.org/wiki/Q14881255","display_name":"Prion protein","level":3,"score":0.6627962589263916},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5228503346443176},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3447359502315521},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.16952884197235107},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.15324151515960693},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.10128355026245117},{"id":"https://openalex.org/C2779134260","wikidata":"https://www.wikidata.org/wiki/Q12136","display_name":"Disease","level":2,"score":0.0},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.0},{"id":"https://openalex.org/C99454951","wikidata":"https://www.wikidata.org/wiki/Q932068","display_name":"Environmental health","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:repositorio.ulisboa.pt:10451/11853","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10451/11853","pdf_url":null,"source":{"id":"https://openalex.org/S4306402433","display_name":"Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"master thesis"},{"id":"mag:2231034422","doi":null,"is_oa":false,"landing_page_url":"https://repositorio.ul.pt/bitstream/10451/11853/1/ulfc107695_tm_leonor_ferreira.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:repositorio.ulisboa.pt:10451/11853","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10451/11853","pdf_url":null,"source":{"id":"https://openalex.org/S4306402433","display_name":"Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"master thesis"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.4000000059604645,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":80,"referenced_works":["https://openalex.org/W33695110","https://openalex.org/W148098798","https://openalex.org/W1530759570","https://openalex.org/W1535883711","https://openalex.org/W1540123986","https://openalex.org/W1550540174","https://openalex.org/W1658462258","https://openalex.org/W1704732331","https://openalex.org/W1829344915","https://openalex.org/W1950949146","https://openalex.org/W1964562627","https://openalex.org/W1966464832","https://openalex.org/W1966825036","https://openalex.org/W1973592629","https://openalex.org/W1974764248","https://openalex.org/W1975687397","https://openalex.org/W1979048283","https://openalex.org/W1979695510","https://openalex.org/W1984444880","https://openalex.org/W1985944494","https://openalex.org/W1986563344","https://openalex.org/W1990290617","https://openalex.org/W1990610956","https://openalex.org/W1992111988","https://openalex.org/W1998059695","https://openalex.org/W2001027895","https://openalex.org/W2002402374","https://openalex.org/W2005519900","https://openalex.org/W2005727122","https://openalex.org/W2007687228","https://openalex.org/W2009546460","https://openalex.org/W2011825403","https://openalex.org/W2012109320","https://openalex.org/W2014049347","https://openalex.org/W2014138300","https://openalex.org/W2017342569","https://openalex.org/W2018608889","https://openalex.org/W2019996679","https://openalex.org/W2020258831","https://openalex.org/W2020898625","https://openalex.org/W2027907160","https://openalex.org/W2029812151","https://openalex.org/W2035068806","https://openalex.org/W2035795655","https://openalex.org/W2038744917","https://openalex.org/W2043400793","https://openalex.org/W2045309285","https://openalex.org/W2047700774","https://openalex.org/W2052981776","https://openalex.org/W2058664657","https://openalex.org/W2058864686","https://openalex.org/W2063735329","https://openalex.org/W2065873129","https://openalex.org/W2067790238","https://openalex.org/W2071178358","https://openalex.org/W2077118428","https://openalex.org/W2080222136","https://openalex.org/W2103658478","https://openalex.org/W2104455526","https://openalex.org/W2106306100","https://openalex.org/W2108072126","https://openalex.org/W2108180175","https://openalex.org/W2108645326","https://openalex.org/W2110720522","https://openalex.org/W2120030908","https://openalex.org/W2127185099","https://openalex.org/W2128945609","https://openalex.org/W2129147752","https://openalex.org/W2130486633","https://openalex.org/W2130547480","https://openalex.org/W2135327444","https://openalex.org/W2135829328","https://openalex.org/W2138392822","https://openalex.org/W2143378615","https://openalex.org/W2150456208","https://openalex.org/W2159525719","https://openalex.org/W2160142215","https://openalex.org/W2167884034","https://openalex.org/W2348951445","https://openalex.org/W4388157634"],"related_works":["https://openalex.org/W2089019621","https://openalex.org/W2120030908","https://openalex.org/W1979695510","https://openalex.org/W2110720522","https://openalex.org/W7098503097","https://openalex.org/W1986563344","https://openalex.org/W2098401678","https://openalex.org/W2104455526","https://openalex.org/W33942336","https://openalex.org/W7018052554"],"abstract_inverted_index":{"O":[0,54,375],"gene":[1,132,160,295,326,431,465,529],"PRNP":[2],"\u00e9":[3,56,81,90,516],"respons\u00e1vel":[4,57],"pela":[5,58,455],"express\u00e3o":[6,59,130,143,158,184,406,572,605],"da":[7,49,69,126,129,251,277,279,283,360,362,412,484,571,592,603],"prote\u00edna":[8,62,79,201,252,272,314,551,574,593,617],"pri\u00f3nica":[9,64],"celular":[10],"(PrPC),":[11],"que,":[12],"ap\u00f3s":[13,488,606],"sofrer":[14],"uma":[15,61,289,569,612],"convers\u00e3o":[16],"p\u00f3s-tradu\u00e7\u00e3o":[17],"numa":[18],"isoforma":[19],"anormal":[20],"e":[21,28,99,107,121,189,209,235,282,298,303,334,336,340,354,366,371,396,420,437,473,537,631],"insol\u00favel":[22],"(PrPSc),":[23],"est\u00e1":[24,72],"associada":[25,73],"\u00e0":[26,29,74,87,166,170,373,398],"transmiss\u00e3o":[27],"patog\u00e9nese":[30],"das":[31,146,225],"encefalopatias":[32],"espongiformes":[33],"transmiss\u00edveis":[34],"(EETs).":[35],"Mais":[36],"recentemente":[37],"descobriu-se":[38],"um":[39,204,216,474],"outro":[40,177],"gene,":[41],"o":[42,196,425,482,494,607],"PRND,":[43],"que":[44,66,197,199,248,519,589],"se":[45],"julga":[46],"ter":[47,203,273],"resultado":[48],"duplica\u00e7\u00e3o":[50],"ancestral":[51],"do":[52,117,131,174,294,330,427,430,464,486,497,506,522,525,528,535],"PRNP.":[53],"PRND":[55,133,296,327,432,466,530],"de":[60,76,100,111,139,157,185,222,230,236,256,261,265,301,304,368,387,394,401,407,422,434,438,440,477,540,557,567,581,609,626,637,640],"tipo":[63],"Doppel,":[65,186],"ao":[67,144,254,275,383,391,503,579],"contr\u00e1rio":[68],"PrPSc,":[70],"n\u00e3o":[71,164,546],"propaga\u00e7\u00e3o":[75],"EETs.":[77],"A":[78,405],"Doppel":[80,253,408,552,594],"pouco":[82],"expressa":[83,91],"no":[84,92,325,470,635],"c\u00e9rebro,":[85],"contrariamente":[86],"PrPC,":[88],"mas":[89,168,191,227],"cora\u00e7\u00e3o":[93],"e,":[94],"principalmente,":[95],"nas":[96,109,114,137],"c\u00e9lulas":[97,110,115,138,147],"germinativas":[98,148],"Sertoli":[101],"(ratinho,":[102],"ratazana,":[103],"su\u00ednos,":[104],"humanos,":[105],"ovinos":[106,577],"bovinos),":[108],"Leydig":[112],"(caprinos),":[113],"epiteliais":[116],"epid\u00eddimo":[118],"(javali,":[119],"su\u00ednos),":[120],"nos":[122,347],"fol\u00edculos":[123],"ov\u00e1ricos":[124],"(c\u00e9lulas":[125],"granulosa).":[127],"Apesar":[128],"aparentemente":[134],"ser":[135],"constante":[136],"Sertoli,":[140],"a":[141,232,249,259,269,299,310,338,489,520,533,538,550,563,590,601],"sua":[142,399,604],"n\u00edvel":[145,276],"masculinas":[149],"parece":[150],"variar":[151],"bastante.":[152],"Como":[153],"tal,":[154],"diferentes":[155,348],"padr\u00f5es":[156],"deste":[159,452,511],"podem":[161],"estar":[162],"associados":[163],"s\u00f3":[165,547],"esp\u00e9cie,":[167],"tamb\u00e9m":[169,379,417,561],"fertilidade":[171,210,284,619],"ou":[172,634],"infertilidade":[173],"macho.":[175],"Por":[176],"lado,":[178],"os":[179,192,238,292],"ratinhos":[180],"f\u00eameas":[181],"PRNDknockout,":[182],"sem":[183,228],"s\u00e3o":[187,194],"vi\u00e1veis":[188],"f\u00e9rteis,":[190],"machos":[193,213,627],"est\u00e9reis,":[195],"sugere":[198],"esta":[200],"possa":[202],"papel":[205],"importante":[206,613],"na":[207,242,459,618,624],"gametog\u00e9nese":[208],"masculina.":[211,285],"Os":[212,450],"PRND-knockout":[214],"t\u00eam":[215],"comportamento":[217],"sexual":[218],"normal,":[219],"com":[220,345,424,493,628],"concentra\u00e7\u00f5es":[221],"espermatoz\u00f3ides":[223,266,344,388,411,576,597],"pr\u00f3ximas":[224],"normais,":[226],"capacidade":[229,260,300,400,632],"realizar":[231],"reac\u00e7\u00e3o":[233],"across\u00f3mica":[234,281],"fertilizar":[237],"o\u00f3citos":[239],"(n\u00e3o":[240],"penetram":[241],"zona":[243],"pel\u00facida).":[244],"Estudos":[245],"recentes":[246],"demonstram":[247],"adi\u00e7\u00e3o":[250],"meio":[255],"capacita\u00e7\u00e3o":[257,395],"aumenta":[258],"fertiliza\u00e7\u00e3o":[262,302,402],"in":[263,403],"vitro":[264],"ovinos,":[267,543],"refor\u00e7ando":[268],"hip\u00f3tese":[270],"desta":[271,313,573,616],"fun\u00e7\u00f5es":[274],"regula\u00e7\u00e3o":[278],"fun\u00e7\u00e3o":[280,311,614],"Resultados":[286],"anteriores":[287],"evidenciaram":[288],"associa\u00e7\u00e3o":[290],"entre":[291],"polimorfismos":[293,324,349,463,469],"ovino":[297,377,409,531],"crio-resist\u00eancia":[305,534,630],"dos":[306,342],"espermatoz\u00f3ides.":[307],"No":[308],"entanto,":[309],"espec\u00edfica":[312],"permanece":[315],"por":[316,565],"esclarecer.":[317],"Este":[318,584],"trabalho":[319,453,585],"tem":[320],"como":[321,481,560,600],"objectivo":[322],"identificar":[323,549],"em":[328,410,491,553,575,596],"indiv\u00edduos":[329],"sexo":[331],"masculino":[332],"(humanos":[333],"ovinos)":[335],"comparar":[337],"qualidade":[339],"crioresist\u00eancia":[341],"seus":[343],"base":[346],"encontrados":[350],"(humano:":[351],"cod\u00e3o":[352,357,428,471,498,526],"174":[353,472,499],"3\u2019UTR;":[355],"ovino:":[356],"26)":[358],"atrav\u00e9s":[359,433,556],"an\u00e1lise":[361],"motilidade,":[363],"vitalidade,":[364],"concentra\u00e7\u00e3o":[365],"morfologia":[367],"s\u00e9men":[369,376,487,536,554],"fresco":[370],"submetido":[372],"criopreserva\u00e7\u00e3o.":[374],"crio-preservado":[378],"foi":[380,418],"avaliado":[381],"quanto":[382,390,397],"potencial":[384],"mitocondrial":[385],"transmembranar":[386],"p\u00f3s-swim-up,":[389],"seu":[392],"estadio":[393],"vitro.":[404],"ra\u00e7a":[413],"Churra":[414],"Galega":[415],"Mirandesa":[416],"caracterizada":[419],"quantificada":[421],"acordo":[423],"gen\u00f3tipo":[426,495,504,523],"26":[429,527],"imunofluoresc\u00eancia":[435,558],"indirecta":[436],"citometria":[439,566],"fluxo,":[441,568],"usando":[442],"anticorpos":[443],"monoclonais":[444],"produzidos":[445],"especificamente":[446],"contra":[447],"este":[448],"p\u00e9ptido.":[449],"resultados":[451,545],"permitiram,":[454],"primeira":[456],"vez,":[457],"detectar":[458],"popula\u00e7\u00e3o":[460],"portuguesa":[461],"tr\u00eas":[462],"humano":[467],"(dois":[468],"38":[475],"pares":[476],"bases":[478],"3\u2019UTR),":[479],"bem":[480,599],"aumento":[483],"viabilidade":[485],"descongela\u00e7\u00e3o":[490],"dadores":[492],"CT":[496],"(gen\u00f3tipo":[500,509],"wildtype)":[501],"associado":[502],"TT":[505],"polimorfismo":[507],"3\u2019UTR":[508],"wild-type)":[510],"gene.":[512],"Para":[513],"al\u00e9m":[514],"disto,":[515],"poss\u00edvel":[517],"concluir":[518,588],"presen\u00e7a":[521],"AA":[524],"melhora":[532],"produ\u00e7\u00e3o":[539],"embri\u00f5es.":[541],"Em":[542],"estes":[544],"permitiram":[548,562],"fresco,":[555],"indirecta,":[559],"detectar,":[564],"diminui\u00e7\u00e3o":[570,602],"submetidos":[578],"processo":[580,608],"criopreserva\u00e7\u00e3o":[582],".":[583],"permitiu":[586],"ainda":[587],"identifica\u00e7\u00e3o":[591],"ovina":[595],"frescos,":[598],"criopreserva\u00e7\u00e3o,":[610],"revelam":[611],"fisiol\u00f3gica":[615],"masculina,":[620],"abrindo":[621],"novos":[622],"horizontes":[623],"selec\u00e7\u00e3o":[625],"melhor":[629],"reprodutiva,":[633],"desenvolvimento":[636],"tratamentos":[638],"cl\u00ednicos":[639],"infertilidade.":[641]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
