{"id":"https://openalex.org/W2228575296","doi":null,"title":"Construction of Saturated Mutation Library for \u03b2-Galactosidase and a Rapid Screening Method for Mutants with High Activity","display_name":"Construction of Saturated Mutation Library for \u03b2-Galactosidase and a Rapid Screening Method for Mutants with High Activity","publication_year":2014,"publication_date":"2014-01-01","ids":{"openalex":"https://openalex.org/W2228575296","mag":"2228575296"},"language":"en","primary_location":{"id":"mag:2228575296","doi":null,"is_oa":false,"landing_page_url":"https://www.cabdirect.org/cabdirect/abstract/20143172757","pdf_url":null,"source":{"id":"https://openalex.org/S4306515304","display_name":"Zhongguo nongye ke-ji daobao","issn_l":"1008-0864","issn":["1008-0864"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Zhongguo nongye ke-ji daobao","raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5141559749","display_name":"Xing Qin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210138501","display_name":"Chinese Academy of Agricultural Sciences","ror":"https://ror.org/0313jb750","country_code":"CN","type":"government","lineage":["https://openalex.org/I4210127390","https://openalex.org/I4210138501","https://openalex.org/I4210151987"]},{"id":"https://openalex.org/I4210167153","display_name":"Biotechnology Research Institute","ror":"https://ror.org/05vm76w92","country_code":"CN","type":"facility","lineage":["https://openalex.org/I4210127390","https://openalex.org/I4210138501","https://openalex.org/I4210151987","https://openalex.org/I4210167153"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xing Qin","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5141684933","display_name":"Sun Ning","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Sun Ning","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100738620","display_name":"Fenghua Zhang","orcid":"https://orcid.org/0000-0002-1666-9548","observed_orcids":["https://orcid.org/0000-0002-1666-9548"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Fenghua Zhang","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-1666-9548","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101426409","display_name":"Yuhong Zhang","orcid":"https://orcid.org/0009-0002-6704-5919","observed_orcids":["https://orcid.org/0009-0002-6704-5919"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Yuhong Zhang","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0009-0002-6704-5919","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5141482628","display_name":"Wu Kun","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Wu Kun","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100609021","display_name":"Wei Zhang","orcid":"https://orcid.org/0000-0003-2154-2485","observed_orcids":["https://orcid.org/0000-0003-2154-2485"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Wei Zhang","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-2154-2485","affiliations":[]},{"author_position":"last","author":{"id":null,"display_name":"Bo Liu","orcid":"https://orcid.org/0000-0003-1685-7968","observed_orcids":["https://orcid.org/0000-0003-1685-7968"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Bo Liu","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-1685-7968","affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"16","issue":"2","first_page":"135","last_page":"142"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.5396000146865845,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.5396000146865845,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.44510000944137573,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10835","display_name":"Carbohydrate Chemistry and Synthesis","score":0.0019000000320374966,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/beta-galactosidase","display_name":"beta-galactosidase","score":0.9300000071525574},{"id":"https://openalex.org/keywords/mutant-screening","display_name":"mutant screening","score":0.7089999914169312},{"id":"https://openalex.org/keywords/enzyme-activity","display_name":"enzyme activity","score":0.781000018119812}],"concepts":[{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.6529300212860107},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.5799945592880249},{"id":"https://openalex.org/C163565370","wikidata":"https://www.wikidata.org/wiki/Q4308623","display_name":"Mutation testing","level":4,"score":0.5310186743736267},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.37631309032440186},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.36900150775909424},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.364474892616272},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.33387261629104614},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2931945323944092},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.15605652332305908}],"mesh":[],"locations_count":2,"locations":[{"id":"mag:2228575296","doi":null,"is_oa":false,"landing_page_url":"https://www.cabdirect.org/cabdirect/abstract/20143172757","pdf_url":null,"source":{"id":"https://openalex.org/S4306515304","display_name":"Zhongguo nongye ke-ji daobao","issn_l":"1008-0864","issn":["1008-0864"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Zhongguo nongye ke-ji daobao","raw_type":null},{"id":"mag:3146874803","doi":null,"is_oa":false,"landing_page_url":"https://en.cnki.com.cn/Article_en/CJFDTOTAL-NKDB201402028.htm","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Journal of Agricultural Science and Technology","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W900666882","https://openalex.org/W3203028698","https://openalex.org/W4231809575","https://openalex.org/W1975601145","https://openalex.org/W6927271399","https://openalex.org/W7084405856","https://openalex.org/W4200125924","https://openalex.org/W1964597525","https://openalex.org/W6991562162","https://openalex.org/W7066552952"],"abstract_inverted_index":null,"counts_by_year":[],"updated_date":"2026-09-27T08:07:27.782135","created_date":"2016-06-24T00:00:00"}