{"id":"https://openalex.org/W222187","doi":"https://doi.org/10.1096/fasebj.22.2_supplement.293","title":"Mapping of Contact Sites Between the M3 Muscarinic Acetylcholine Receptor and Gq by Cross\u2010linking Approaches","display_name":"Mapping of Contact Sites Between the M3 Muscarinic Acetylcholine Receptor and Gq by Cross\u2010linking Approaches","publication_year":2008,"publication_date":"2008-04-01","ids":{"openalex":"https://openalex.org/W222187","doi":"https://doi.org/10.1096/fasebj.22.2_supplement.293","mag":"222187"},"language":"en","primary_location":{"id":"doi:10.1096/fasebj.22.2_supplement.293","doi":"https://doi.org/10.1096/fasebj.22.2_supplement.293","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.22.2_supplement.293","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101909762","display_name":"Jianxin Hu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210090567","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210090567"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jianxin Hu","raw_affiliation_strings":["Laboratory of Bioorganic Chemistry/NIDDK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Bioorganic Chemistry/NIDDK","institution_ids":["https://openalex.org/I4210090567"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110670970","display_name":"Jianhua Li","orcid":"https://orcid.org/0000-0001-7418-9465","observed_orcids":["https://orcid.org/0000-0001-7418-9465"]},"institutions":[{"id":"https://openalex.org/I4210090567","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210090567"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jianhua Li","raw_affiliation_strings":["Laboratory of Bioorganic Chemistry/NIDDK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Bioorganic Chemistry/NIDDK","institution_ids":["https://openalex.org/I4210090567"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5021219075","display_name":"Stefano Costanzi","orcid":"https://orcid.org/0000-0003-3183-7332","observed_orcids":["https://orcid.org/0000-0003-3183-7332"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210090567","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210090567"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Stefano Costanzi","raw_affiliation_strings":["Laboratory of Biological Modeling/NIDDK National Institutes of Health Bethesda MD"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Biological Modeling/NIDDK National Institutes of Health Bethesda MD","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210090567"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Xiaohong Zhang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210090567","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210090567"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Xiaohong Zhang","raw_affiliation_strings":["Laboratory of Bioorganic Chemistry/NIDDK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Bioorganic Chemistry/NIDDK","institution_ids":["https://openalex.org/I4210090567"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5015095365","display_name":"J\u00fcrgen Wess","orcid":"https://orcid.org/0000-0003-0818-1232","observed_orcids":["https://orcid.org/0000-0003-0818-1232"]},"institutions":[{"id":"https://openalex.org/I4210090567","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210090567"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jurgen Wess","raw_affiliation_strings":["Laboratory of Bioorganic Chemistry/NIDDK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Bioorganic Chemistry/NIDDK","institution_ids":["https://openalex.org/I4210090567"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3740,"currency":"USD","value_usd":3740},"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"22","issue":"S2","first_page":"293","last_page":"293"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10077","display_name":"Neuroscience and Neuropharmacology Research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/muscarinic-m3-receptor","display_name":"M3 muscarinic receptor","score":0.9990000128746033},{"id":"https://openalex.org/keywords/receptor-g-protein-coupling","display_name":"receptor-G protein coupling","score":0.8999999761581421},{"id":"https://openalex.org/keywords/cysteine-scanning-mutagenesis","display_name":"cysteine scanning mutagenesis","score":0.8309999704360962},{"id":"https://openalex.org/keywords/galphaq","display_name":"G\u03b1q","score":0.8349999785423279},{"id":"https://openalex.org/keywords/g-protein-coupled-receptors","display_name":"G protein-coupled receptors","score":0.7850000262260437}],"concepts":[{"id":"https://openalex.org/C135285700","wikidata":"https://www.wikidata.org/wiki/Q38173","display_name":"G protein-coupled receptor","level":3,"score":0.8814151883125305},{"id":"https://openalex.org/C64615621","wikidata":"https://www.wikidata.org/wiki/Q15333405","display_name":"Muscarinic acetylcholine receptor M5","level":5,"score":0.6496849656105042},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.6160321235656738},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.6147233843803406},{"id":"https://openalex.org/C17971392","wikidata":"https://www.wikidata.org/wiki/Q18027445","display_name":"5-HT5A receptor","level":3,"score":0.5688906908035278},{"id":"https://openalex.org/C207723603","wikidata":"https://www.wikidata.org/wiki/Q17861926","display_name":"Muscarinic acetylcholine receptor M1","level":4,"score":0.5439327955245972},{"id":"https://openalex.org/C2779201268","wikidata":"https://www.wikidata.org/wiki/Q186474","display_name":"Cysteine","level":3,"score":0.529003918170929},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.5223775506019592},{"id":"https://openalex.org/C80631254","wikidata":"https://www.wikidata.org/wiki/Q407438","display_name":"G protein","level":3,"score":0.49986720085144043},{"id":"https://openalex.org/C33789571","wikidata":"https://www.wikidata.org/wiki/Q410959","display_name":"Muscarinic acetylcholine receptor","level":3,"score":0.4693639576435089},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.44890493154525757},{"id":"https://openalex.org/C116289061","wikidata":"https://www.wikidata.org/wiki/Q17861955","display_name":"Muscarinic acetylcholine receptor M3","level":4,"score":0.4344547390937805},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4066659212112427},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.34102463722229004},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.10454005002975464},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1096/fasebj.22.2_supplement.293","doi":"https://doi.org/10.1096/fasebj.22.2_supplement.293","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.22.2_supplement.293","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337357","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2291782253","https://openalex.org/W2010544588","https://openalex.org/W1985529228","https://openalex.org/W2030175837","https://openalex.org/W2021962189","https://openalex.org/W2084413946","https://openalex.org/W2143879590","https://openalex.org/W2034080363","https://openalex.org/W2056312165","https://openalex.org/W4393112185"],"abstract_inverted_index":{"How":[0],"a":[1,66,71,87,103,114],"G":[2,11,51],"protein\u2010coupled":[3],"receptor":[4,93,149],"(GPCR)":[5],"interacts":[6],"specifically":[7],"with":[8],"its":[9],"cognate":[10,50],"protein(s)":[12],"remains":[13],"largely":[14],"unknown.":[15],"Many":[16],"studies":[17,157],"have":[18,166],"identified":[19],"distinct":[20],"GPCR":[21],"and":[22,48,124,150],"G\u03b1":[23],"regions":[24],"that":[25,94],"are":[26],"predicted":[27],"to":[28,59,80,129,158],"be":[29],"critically":[30],"involved":[31],"in":[32],"productive":[33],"receptor/G":[34],"protein":[35,193],"coupling.":[36],"However,":[37],"little":[38],"is":[39],"known":[40],"about":[41],"the":[42,61,91,179,188],"specific":[43,171],"point\u2010to\u2010point":[44],"interactions":[45],"between":[46],"GPCRs":[47],"their":[49],"proteins.":[52],"To":[53],"address":[54],"this":[55],"question,":[56],"we":[57],"chose":[58],"study":[60],"M3":[62,92,122,148,172,180],"muscarinic":[63],"acetylcholine":[64],"receptor,":[65,69],"prototypic":[67],"Gq\u2010coupled":[68],"as":[70,113],"model":[72],"system.":[73],"Cysteine":[74],"(Cys)":[75],"scanning":[76],"mutagenesis":[77],"was":[78],"applied":[79],"introduce":[81],"thiol":[82],"groups":[83],"for":[84,116,160],"cross\u2010linking":[85,156],"into":[86,187],"mutant":[88,104,121],"version":[89,105],"of":[90,106,146,178,191],"lacked":[95],"most":[96,109],"endogenous":[97],"Cys":[98,111,117,162],"residues.":[99],"We":[100],"also":[101],"generated":[102],"G\u03b1q":[107,125,151],"lacking":[108],"native":[110],"residues":[112],"template":[115],"mutagenesis.":[118],"The":[119],"Cys\u2010substituted":[120,147],"receptors":[123],"proteins":[126],"were":[127,137],"verified":[128],"remain":[130],"functional":[131],"by":[132,154],"established":[133],"biochemical":[134],"techniques.":[135],"Membranes":[136],"prepared":[138],"from":[139],"transfected":[140],"COS\u20107":[141],"cells":[142],"co\u2010expressing":[143],"various":[144],"combinations":[145],"constructs,":[152],"followed":[153],"Cys\u2010Cys":[155],"screen":[159],"vicinal":[161],"pairs.":[163],"Initial":[164],"experiments":[165],"yielded":[167],"interesting":[168],"results":[169],"regarding":[170],"receptor/G\u03b1q":[173,181],"contact":[174],"sites.":[175],"Further":[176],"mapping":[177],"interface":[182],"should":[183],"provide":[184],"detailed":[185],"insights":[186],"molecular":[189],"basis":[190],"receptor\u2010G":[192],"interactions.":[194]},"counts_by_year":[],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2016-06-24T00:00:00"}