{"id":"https://openalex.org/W22154320","doi":"https://doi.org/10.4049/jimmunol.182.supp.78.14","title":"APLP2 Diverts MHC-Peptide Complexes to Clathrin-Mediated Endocytosis and Lysosomal Degradation (78.14)","display_name":"APLP2 Diverts MHC-Peptide Complexes to Clathrin-Mediated Endocytosis and Lysosomal Degradation (78.14)","publication_year":2009,"publication_date":"2009-04-01","ids":{"openalex":"https://openalex.org/W22154320","doi":"https://doi.org/10.4049/jimmunol.182.supp.78.14","mag":"22154320"},"language":"en","primary_location":{"id":"doi:10.4049/jimmunol.182.supp.78.14","doi":"https://doi.org/10.4049/jimmunol.182.supp.78.14","is_oa":false,"landing_page_url":"https://doi.org/10.4049/jimmunol.182.supp.78.14","pdf_url":null,"source":{"id":"https://openalex.org/S38008053","display_name":"The Journal of Immunology","issn_l":"0022-1767","issn":["0022-1767","1550-6606"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4320800377","host_organization_name":"American Association of Immunologists","host_organization_lineage":["https://openalex.org/P4320800377"],"host_organization_lineage_names":["American Association of Immunologists"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Immunology","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","is_traditional":false,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5068379719","display_name":"Amit Tuli","orcid":"https://orcid.org/0000-0001-5476-4127","observed_orcids":["https://orcid.org/0000-0001-5476-4127"]},"institutions":[{"id":"https://openalex.org/I59130452","display_name":"University of Nebraska Medical Center","ror":"https://ror.org/00thqtb16","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I59130452"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Amit Tuli","raw_affiliation_strings":["Department of Biochemistry and Molecular Biology","Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center , Omaha, Nebraska","1Department of Biochemistry and Molecular Biology","2Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Molecular Biology","institution_ids":[]},{"raw_affiliation_string":"Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center , Omaha, Nebraska","institution_ids":["https://openalex.org/I59130452"]},{"raw_affiliation_string":"1Department of Biochemistry and Molecular Biology","institution_ids":[]},{"raw_affiliation_string":"2Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska","institution_ids":["https://openalex.org/I59130452"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038205163","display_name":"Mahak Sharma","orcid":"https://orcid.org/0000-0002-4117-4790","observed_orcids":["https://orcid.org/0000-0002-4117-4790"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Mahak Sharma","raw_affiliation_strings":["Department of Biochemistry and Molecular Biology","1Department of Biochemistry and Molecular Biology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Molecular Biology","institution_ids":[]},{"raw_affiliation_string":"1Department of Biochemistry and Molecular Biology","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012561771","display_name":"Haley L. Capek","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I59130452","display_name":"University of Nebraska Medical Center","ror":"https://ror.org/00thqtb16","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I59130452"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Haley Capek","raw_affiliation_strings":["Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center , Omaha, Nebraska","2Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center , Omaha, Nebraska","institution_ids":["https://openalex.org/I59130452"]},{"raw_affiliation_string":"2Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska","institution_ids":["https://openalex.org/I59130452"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5085276533","display_name":"Naava Naslavsky","orcid":"https://orcid.org/0009-0004-0965-3881","observed_orcids":["https://orcid.org/0009-0004-0965-3881"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Naava Naslavsky","raw_affiliation_strings":["Department of Biochemistry and Molecular Biology","1Department of Biochemistry and Molecular Biology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Molecular Biology","institution_ids":[]},{"raw_affiliation_string":"1Department of Biochemistry and Molecular Biology","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5004821340","display_name":"Steve Caplan","orcid":"https://orcid.org/0000-0001-9445-4297","observed_orcids":["https://orcid.org/0000-0001-9445-4297"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Steve Caplan","raw_affiliation_strings":["Department of Biochemistry and Molecular Biology","1Department of Biochemistry and Molecular Biology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Molecular Biology","institution_ids":[]},{"raw_affiliation_string":"1Department of Biochemistry and Molecular Biology","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5086505077","display_name":"Joyce C. Solheim","orcid":"https://orcid.org/0000-0002-6313-8213","observed_orcids":["https://orcid.org/0000-0002-6313-8213"]},"institutions":[{"id":"https://openalex.org/I59130452","display_name":"University of Nebraska Medical Center","ror":"https://ror.org/00thqtb16","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I59130452"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Joyce Solheim","raw_affiliation_strings":["Department of Biochemistry and Molecular Biology","Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center , Omaha, Nebraska","1Department of Biochemistry and Molecular Biology","2Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Molecular Biology","institution_ids":[]},{"raw_affiliation_string":"Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center , Omaha, Nebraska","institution_ids":["https://openalex.org/I59130452"]},{"raw_affiliation_string":"1Department of Biochemistry and Molecular Biology","institution_ids":[]},{"raw_affiliation_string":"2Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska","institution_ids":["https://openalex.org/I59130452"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3500,"currency":"USD","value_usd":3500},"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"182","issue":"Supplement_1","first_page":"78.14","last_page":"78.14"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10086","display_name":"Alzheimer's disease research and treatments","score":0.0012000000569969416,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10587","display_name":"Autophagy in Disease and Therapy","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/aplp2","display_name":"APLP2","score":0.9390000104904175},{"id":"https://openalex.org/keywords/mhc-class-i","display_name":"MHC class I","score":0.7089999914169312},{"id":"https://openalex.org/keywords/h-2kd","display_name":"H-2Kd","score":0.9869999885559082},{"id":"https://openalex.org/keywords/clathrin-mediated-endocytosis","display_name":"clathrin-mediated endocytosis","score":0.949999988079071},{"id":"https://openalex.org/keywords/lysosomal-degradation","display_name":"lysosomal degradation","score":0.8730000257492065},{"id":"https://openalex.org/keywords/peptide-mhc-complex","display_name":"peptide-MHC complex","score":0.7239999771118164},{"id":"https://openalex.org/keywords/tyrosine-based-motif","display_name":"tyrosine-based motif","score":0.7570000290870667},{"id":"https://openalex.org/keywords/dynamin-ii","display_name":"dynamin II","score":0.8970000147819519},{"id":"https://openalex.org/keywords/ap180","display_name":"AP180","score":0.9300000071525574},{"id":"https://openalex.org/keywords/protein-internalization","display_name":"protein internalization","score":0.6589999794960022},{"id":"https://openalex.org/keywords/plasma-membrane-trafficking","display_name":"plasma membrane trafficking","score":0.5759999752044678}],"concepts":[{"id":"https://openalex.org/C28005876","wikidata":"https://www.wikidata.org/wiki/Q189814","display_name":"Endocytosis","level":3,"score":0.9002073407173157},{"id":"https://openalex.org/C63162447","wikidata":"https://www.wikidata.org/wiki/Q21100804","display_name":"Clathrin","level":4,"score":0.7233138680458069},{"id":"https://openalex.org/C139770010","wikidata":"https://www.wikidata.org/wiki/Q1339807","display_name":"Internalization","level":3,"score":0.7097750902175903},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.6055701375007629},{"id":"https://openalex.org/C156407911","wikidata":"https://www.wikidata.org/wiki/Q420160","display_name":"Dynamin","level":4,"score":0.5755842924118042},{"id":"https://openalex.org/C102747710","wikidata":"https://www.wikidata.org/wiki/Q824237","display_name":"Endosome","level":3,"score":0.5639453530311584},{"id":"https://openalex.org/C117943116","wikidata":"https://www.wikidata.org/wiki/Q1135604","display_name":"Receptor-mediated endocytosis","level":4,"score":0.4899989664554596},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.44959110021591187},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.39283281564712524},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3836187720298767},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.2025170922279358}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.4049/jimmunol.182.supp.78.14","doi":"https://doi.org/10.4049/jimmunol.182.supp.78.14","is_oa":false,"landing_page_url":"https://doi.org/10.4049/jimmunol.182.supp.78.14","pdf_url":null,"source":{"id":"https://openalex.org/S38008053","display_name":"The Journal of Immunology","issn_l":"0022-1767","issn":["0022-1767","1550-6606"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4320800377","host_organization_name":"American Association of Immunologists","host_organization_lineage":["https://openalex.org/P4320800377"],"host_organization_lineage_names":["American Association of Immunologists"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Immunology","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.8048999905586243,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1967040338","https://openalex.org/W1533529945","https://openalex.org/W84123251","https://openalex.org/W2072566106","https://openalex.org/W2017127553","https://openalex.org/W1974156090","https://openalex.org/W1969686812","https://openalex.org/W2094028293","https://openalex.org/W2136101171","https://openalex.org/W4313333710"],"abstract_inverted_index":{"Abstract":[0],"The":[1],"amyloid":[2],"precursor-like":[3],"protein":[4,10,113],"2":[5],"(APLP2)":[6],"is":[7,12,44,75],"a":[8,47,51,95,179,194,210],"secreted":[9],"that":[11,19,42,86,107,144],"ubiquitously":[13],"expressed.":[14],"We":[15],"have":[16],"shown":[17],"previously":[18],"APLP2":[20,43,100,128,145,160,183,208],"associates":[21],"with":[22,82],"peptide-bound":[23],"forms":[24],"of":[25,36,61,65,73,116,125,138,191,218],"H-2Kd,":[26],"and":[27,34,57,146,154,197],"influences":[28,215],"the":[29,58,99,117,136,150,168,174,187,199,203,216,222],"endocytosis,":[30],"surface":[31],"expression,":[32,161],"stability,":[33],"turnover":[35],"Kd.":[37],"Our":[38],"new":[39],"findings":[40,83,177],"indicate":[41],"internalized":[45,92,156],"in":[46,119,181,193],"clathrin-dependent":[48,66,195],"manner,":[49,196],"as":[50],"dominant":[52],"negative":[53],"dynamin":[54],"II":[55],"mutant":[56],"C-terminal":[59],"tail":[60,102],"AP180":[62],"(both":[63],"inhibitors":[64],"endocytosis)":[67],"block":[68],"its":[69],"internalization.":[70],"However,":[71],"endocytosis":[72,124,137],"Kd":[74,139,147,162,185,192,201],"not":[76],"blocked":[77],"by":[78,84],"these":[79],"inhibitors,":[80],"consistent":[81],"others":[85],"MHC":[87],"class":[88],"I":[89],"molecules":[90,163],"are":[91,155],"mainly":[93],"through":[94],"clathrin-independent":[96],"pathway.":[97,206],"Furthermore,":[98],"cytoplasmic":[101],"contains":[103],"overlapping":[104],"tyrosine-based":[105],"motifs":[106],"can":[108],"potentially":[109],"bind":[110,148],"to":[111,134,167,173,202],"adaptor":[112],"AP-2.":[114],"Mutation":[115],"tyrosine":[118],"this":[120],"sequence":[121],"severely":[122],"impaired":[123],"APLP2.":[126],"This":[127],"mutant,":[129],"unlike":[130],"wild-type":[131],"APLP2,":[132],"failed":[133],"enhance":[135],"molecules.":[140],"Also,":[141],"we":[142],"found":[143],"at":[149,186,221],"plasma":[151],"membrane":[152],"(PM)":[153],"together.":[157],"Upon":[158],"increased":[159],"were":[164],"predominantly":[165],"directed":[166],"lysosomes":[169],"rather":[170],"than":[171],"recycled":[172],"PM.":[175,223],"These":[176],"suggest":[178],"model":[180],"which":[182,214],"binds":[184],"PM,":[188],"facilitates":[189],"uptake":[190],"routes":[198],"endocytosed":[200],"lysosomal":[204],"degradation":[205],"Thus,":[207],"has":[209],"multi-step":[211],"trafficking":[212],"function":[213],"expression":[217],"MHC-peptide":[219],"complexes":[220],"[NIH":[224],"GM57428":[225],"&":[226],"GM74876]":[227]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}