{"id":"https://openalex.org/W2201135677","doi":"https://doi.org/10.1080/105172301300128880","title":"SPECTROPHOTOMETRIC ASSAY FOR ACETANILIDE 4-HYDROXYLASE, AN ESTIMATE OF CYP1A2 ENZYME ACTIVITY","display_name":"SPECTROPHOTOMETRIC ASSAY FOR ACETANILIDE 4-HYDROXYLASE, AN ESTIMATE OF CYP1A2 ENZYME ACTIVITY","publication_year":2001,"publication_date":"2001-01-01","ids":{"openalex":"https://openalex.org/W2201135677","doi":"https://doi.org/10.1080/105172301300128880","mag":"2201135677"},"language":"en","primary_location":{"id":"doi:10.1080/105172301300128880","doi":"https://doi.org/10.1080/105172301300128880","is_oa":false,"landing_page_url":"https://doi.org/10.1080/105172301300128880","pdf_url":null,"source":{"id":"https://openalex.org/S48645365","display_name":"Toxicology Methods","issn_l":"1051-7235","issn":["1051-7235","1091-7667"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Toxicology Methods","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5025906356","display_name":"Howard G. Shertzer","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I92156820","display_name":"University of Cincinnati Medical Center","ror":"https://ror.org/02p72h367","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I92156820"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Howard G. Shertzer","raw_affiliation_strings":["Department of Environmental Health and Center for Environmental Genetics, University of Cincinnati Medical Center, Cincinnati, Ohio, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Environmental Health and Center for Environmental Genetics, University of Cincinnati Medical Center, Cincinnati, Ohio, USA","institution_ids":["https://openalex.org/I92156820"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5154682623","display_name":"D. Nebert","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I92156820","display_name":"University of Cincinnati Medical Center","ror":"https://ror.org/02p72h367","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I92156820"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Nebert Daniel","raw_affiliation_strings":["Department of Environmental Health and Center for Environmental Genetics, University of Cincinnati Medical Center, Cincinnati, Ohio, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Environmental Health and Center for Environmental Genetics, University of Cincinnati Medical Center, Cincinnati, Ohio, USA","institution_ids":["https://openalex.org/I92156820"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Dingeldein Albert","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I92156820","display_name":"University of Cincinnati Medical Center","ror":"https://ror.org/02p72h367","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I92156820"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Dingeldein Albert","raw_affiliation_strings":["Department of Environmental Health and Center for Environmental Genetics, University of Cincinnati Medical Center, Cincinnati, Ohio, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Environmental Health and Center for Environmental Genetics, University of Cincinnati Medical Center, Cincinnati, Ohio, USA","institution_ids":["https://openalex.org/I92156820"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5044682034","display_name":"Senft Michael","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I92156820","display_name":"University of Cincinnati Medical Center","ror":"https://ror.org/02p72h367","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I92156820"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Senft Michael","raw_affiliation_strings":["Department of Environmental Health and Center for Environmental Genetics, University of Cincinnati Medical Center, Cincinnati, Ohio, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Environmental Health and Center for Environmental Genetics, University of Cincinnati Medical Center, Cincinnati, Ohio, USA","institution_ids":["https://openalex.org/I92156820"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5044559552","display_name":"Mary Beth Genter","orcid":"https://orcid.org/0000-0002-8862-1092","observed_orcids":["https://orcid.org/0000-0002-8862-1092"]},"institutions":[{"id":"https://openalex.org/I92156820","display_name":"University of Cincinnati Medical Center","ror":"https://ror.org/02p72h367","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I92156820"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Genter Mary Beth","raw_affiliation_strings":["Department of Environmental Health and Center for Environmental Genetics, University of Cincinnati Medical Center, Cincinnati, Ohio, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Environmental Health and Center for Environmental Genetics, University of Cincinnati Medical Center, Cincinnati, Ohio, USA","institution_ids":["https://openalex.org/I92156820"]}]},{"author_position":"last","author":{"id":null,"display_name":"Dalton Timothy","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I92156820","display_name":"University of Cincinnati Medical Center","ror":"https://ror.org/02p72h367","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I92156820"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Dalton Timothy","raw_affiliation_strings":["Department of Environmental Health and Center for Environmental Genetics, University of Cincinnati Medical Center, Cincinnati, Ohio, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Environmental Health and Center for Environmental Genetics, University of Cincinnati Medical Center, Cincinnati, Ohio, USA","institution_ids":["https://openalex.org/I92156820"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5025906356"],"corresponding_institution_ids":["https://openalex.org/I92156820"],"apc_list":null,"apc_paid":null,"fwci":0.5365,"has_fulltext":false,"cited_by_count":7,"citation_normalized_percentile":{"value":0.71620029,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"11","issue":"2","first_page":"81","last_page":"88"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10375","display_name":"Pharmacogenetics and Drug Metabolism","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/3004","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10375","display_name":"Pharmacogenetics and Drug Metabolism","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/3004","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11465","display_name":"Drug-Induced Hepatotoxicity and Protection","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/3004","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10122","display_name":"Toxic Organic Pollutants Impact","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2307","display_name":"Health, Toxicology and Mutagenesis"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cyp1a2","display_name":"CYP1A2","score":0.8809999823570251},{"id":"https://openalex.org/keywords/spectrophotometric-method","display_name":"spectrophotometric assay","score":0.9330000281333923},{"id":"https://openalex.org/keywords/enzyme-activity-estimation","display_name":"enzyme activity estimation","score":0.640999972820282}],"concepts":[{"id":"https://openalex.org/C2777476671","wikidata":"https://www.wikidata.org/wiki/Q421761","display_name":"Acetanilide","level":2,"score":0.9435316324234009},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7631603479385376},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.6816222667694092},{"id":"https://openalex.org/C179998833","wikidata":"https://www.wikidata.org/wiki/Q381233","display_name":"High-performance liquid chromatography","level":2,"score":0.6354787349700928},{"id":"https://openalex.org/C2776923573","wikidata":"https://www.wikidata.org/wiki/Q2294884","display_name":"Derivatization","level":3,"score":0.5803908109664917},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5478876829147339},{"id":"https://openalex.org/C2776568683","wikidata":"https://www.wikidata.org/wiki/Q23005640","display_name":"Moiety","level":2,"score":0.5476303696632385},{"id":"https://openalex.org/C86745063","wikidata":"https://www.wikidata.org/wiki/Q3737406","display_name":"CYP1A2","level":4,"score":0.4775484502315521},{"id":"https://openalex.org/C2777702071","wikidata":"https://www.wikidata.org/wiki/Q130336","display_name":"Phenol","level":2,"score":0.44276851415634155},{"id":"https://openalex.org/C28212737","wikidata":"https://www.wikidata.org/wiki/Q5381543","display_name":"Enzyme assay","level":3,"score":0.4371527433395386},{"id":"https://openalex.org/C40875361","wikidata":"https://www.wikidata.org/wiki/Q2356542","display_name":"Reagent","level":2,"score":0.41612663865089417},{"id":"https://openalex.org/C526171541","wikidata":"https://www.wikidata.org/wiki/Q407693","display_name":"Cytochrome P450","level":3,"score":0.2439749538898468},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2314852774143219},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.2101786732673645}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1080/105172301300128880","doi":"https://doi.org/10.1080/105172301300128880","is_oa":false,"landing_page_url":"https://doi.org/10.1080/105172301300128880","pdf_url":null,"source":{"id":"https://openalex.org/S48645365","display_name":"Toxicology Methods","issn_l":"1051-7235","issn":["1051-7235","1091-7667"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Toxicology Methods","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":18,"referenced_works":["https://openalex.org/W186679341","https://openalex.org/W1963155846","https://openalex.org/W1982589515","https://openalex.org/W1998943812","https://openalex.org/W2002719254","https://openalex.org/W2006668832","https://openalex.org/W2026802456","https://openalex.org/W2027717019","https://openalex.org/W2037008348","https://openalex.org/W2044017822","https://openalex.org/W2061047555","https://openalex.org/W2066026684","https://openalex.org/W2075649289","https://openalex.org/W2082159810","https://openalex.org/W2100648785","https://openalex.org/W2136223951","https://openalex.org/W2402298356","https://openalex.org/W2405773117"],"related_works":["https://openalex.org/W2026802456","https://openalex.org/W2376209156","https://openalex.org/W1747643686","https://openalex.org/W2094706816","https://openalex.org/W1979439067","https://openalex.org/W2061047555","https://openalex.org/W2049358184","https://openalex.org/W4254039497","https://openalex.org/W2072195371","https://openalex.org/W2413404479"],"abstract_inverted_index":{"Acetanilide":[0],"4-hydroxylase":[1,65],"provides":[2],"the":[3,31,35,41,70,74],"most":[4],"reliable":[5],"enzyme":[6],"activity":[7,19],"estimate":[8],"for":[9,37,63],"cytochrome":[10],"P450":[11],"1A2.":[12],"Current":[13],"methods":[14],"used":[15],"to":[16],"assess":[17],"CYP1A2":[18],"employ":[20],"high":[21],"performance":[22],"liquid":[23],"chromatography":[24],"(HPLC)":[25],"or":[26],"radiolabeled":[27],"substrates.":[28],"Drawbacks":[29],"of":[30,69],"HPLC":[32],"method":[33],"include":[34],"need":[36],"expensive":[38],"equipment":[39],"and":[40],"slow":[42],"throughput,":[43],"while":[44],"radioisotope":[45],"use":[46],"is":[47],"associated":[48],"with":[49,80],"health":[50],"hazards":[51],"as":[52,54],"well":[53],"extensive":[55],"record-keeping.":[56],"We":[57],"describe":[58],"a":[59,81],"new":[60],"spectrophotometric":[61],"assay":[62,85],"acetanilide":[64],"using":[66],"acid":[67],"cleavage":[68],"N-acetyl":[71],"moiety":[72],"from":[73],"reaction":[75],"product,":[76],"followed":[77],"by":[78],"derivatization":[79],"phenol":[82],"reagent.":[83],"The":[84],"detects":[86],"0.1":[87],"nmol":[88],"4-hydroxyacetanilide.":[89]},"counts_by_year":[],"updated_date":"2026-10-27T15:51:10.671819","created_date":"2016-06-24T00:00:00"}