{"id":"https://openalex.org/W2193589009","doi":null,"title":"Investigation of the Time Dependent Influence of Extracellular Osmotic Stress on Protein Turnover in Skeletal Muscle Cells","display_name":"Investigation of the Time Dependent Influence of Extracellular Osmotic Stress on Protein Turnover in Skeletal Muscle Cells","publication_year":2015,"publication_date":"2015-09-15","ids":{"openalex":"https://openalex.org/W2193589009","mag":"2193589009"},"language":"en","primary_location":{"id":"pmh:oai:brocku.scholaris.ca:10464/7206","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10464/7206","pdf_url":"https://brocku.scholaris.ca/bitstreams/cf8d8f02-2ffb-489a-9f93-7fffc60267d0/download","source":{"id":"https://openalex.org/S4306401333","display_name":"Brock University Digital Repository (Brock University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I186803428","host_organization_name":"Brock University","host_organization_lineage":["https://openalex.org/I186803428"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Electronic Thesis or Dissertation"},"volume":null,"type":"dissertation","indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://brocku.scholaris.ca/bitstreams/cf8d8f02-2ffb-489a-9f93-7fffc60267d0/download","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Michael C. McAlpine","orcid":"https://orcid.org/0000-0001-7869-7598","observed_orcids":["https://orcid.org/0000-0001-7869-7598"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Michael C. McAlpine","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0001-7869-7598","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11399","display_name":"Muscle metabolism and nutrition","score":0.9922000169754028,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11399","display_name":"Muscle metabolism and nutrition","score":0.9922000169754028,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10441","display_name":"Muscle Physiology and Disorders","score":0.005799999926239252,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hyperosmotic-stress","display_name":"hyperosmotic stress","score":0.8579999804496765},{"id":"https://openalex.org/keywords/hypo-osmotic-stress","display_name":"hypoosmotic stress","score":0.9480000138282776},{"id":"https://openalex.org/keywords/protein-turnover","display_name":"protein turnover","score":0.8550000190734863},{"id":"https://openalex.org/keywords/skeletal-muscle-cells","display_name":"skeletal muscle cells","score":0.8479999899864197},{"id":"https://openalex.org/keywords/l6-myoblasts","display_name":"L6 myoblasts","score":0.824999988079071},{"id":"https://openalex.org/keywords/protein-synthesis","display_name":"protein synthesis","score":0.9789999723434448},{"id":"https://openalex.org/keywords/protein-degradation","display_name":"protein degradation","score":0.9549999833106995},{"id":"https://openalex.org/keywords/p70s6k-phosphorylation","display_name":"p70S6K phosphorylation","score":0.9520000219345093},{"id":"https://openalex.org/keywords/cell-volume","display_name":"cell volume","score":0.6549999713897705},{"id":"https://openalex.org/keywords/tyrosine-incorporation","display_name":"tyrosine incorporation","score":0.8939999938011169}],"concepts":[{"id":"https://openalex.org/C28406088","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Extracellular","level":2,"score":0.7088934183120728},{"id":"https://openalex.org/C2779959927","wikidata":"https://www.wikidata.org/wiki/Q1048687","display_name":"Skeletal muscle","level":2,"score":0.5610024929046631},{"id":"https://openalex.org/C195580219","wikidata":"https://www.wikidata.org/wiki/Q7251511","display_name":"Protein turnover","level":3,"score":0.5539422035217285},{"id":"https://openalex.org/C168529131","wikidata":"https://www.wikidata.org/wiki/Q13656562","display_name":"Osmotic shock","level":3,"score":0.47637006640434265},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4595997929573059},{"id":"https://openalex.org/C21036866","wikidata":"https://www.wikidata.org/wiki/Q181767","display_name":"Stress (linguistics)","level":2,"score":0.44225937128067017},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.42700910568237305},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.32626980543136597},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2913617491722107},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.24214401841163635},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.24198463559150696},{"id":"https://openalex.org/C3675279","wikidata":"https://www.wikidata.org/wiki/Q211935","display_name":"Protein biosynthesis","level":2,"score":0.16074919700622559},{"id":"https://openalex.org/C41895202","wikidata":"https://www.wikidata.org/wiki/Q8162","display_name":"Linguistics","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C138885662","wikidata":"https://www.wikidata.org/wiki/Q5891","display_name":"Philosophy","level":0,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:brocku.scholaris.ca:10464/7206","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10464/7206","pdf_url":"https://brocku.scholaris.ca/bitstreams/cf8d8f02-2ffb-489a-9f93-7fffc60267d0/download","source":{"id":"https://openalex.org/S4306401333","display_name":"Brock University Digital Repository (Brock University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I186803428","host_organization_name":"Brock University","host_organization_lineage":["https://openalex.org/I186803428"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Electronic Thesis or Dissertation"},{"id":"mag:2193589009","doi":null,"is_oa":false,"landing_page_url":"https://dr.library.brocku.ca/bitstream/10464/7206/2/Brock_McAlpine_Michael_2015.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:brocku.scholaris.ca:10464/7206","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10464/7206","pdf_url":"https://brocku.scholaris.ca/bitstreams/cf8d8f02-2ffb-489a-9f93-7fffc60267d0/download","source":{"id":"https://openalex.org/S4306401333","display_name":"Brock University Digital Repository (Brock University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I186803428","host_organization_name":"Brock University","host_organization_lineage":["https://openalex.org/I186803428"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Electronic Thesis or Dissertation"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2193589009.pdf","grobid_xml":"https://content.openalex.org/works/W2193589009.grobid-xml"},"referenced_works_count":187,"referenced_works":["https://openalex.org/W4511272","https://openalex.org/W65533086","https://openalex.org/W79354124","https://openalex.org/W89764641","https://openalex.org/W98528490","https://openalex.org/W101880175","https://openalex.org/W139123032","https://openalex.org/W283379564","https://openalex.org/W1226527519","https://openalex.org/W1481845508","https://openalex.org/W1492555480","https://openalex.org/W1495980998","https://openalex.org/W1536486871","https://openalex.org/W1566734962","https://openalex.org/W1586159393","https://openalex.org/W1587891756","https://openalex.org/W1590930560","https://openalex.org/W1726025741","https://openalex.org/W1762734860","https://openalex.org/W1767790527","https://openalex.org/W1816748024","https://openalex.org/W1860092817","https://openalex.org/W1927258044","https://openalex.org/W1937973444","https://openalex.org/W1941910566","https://openalex.org/W1965611875","https://openalex.org/W1970977779","https://openalex.org/W1973293485","https://openalex.org/W1973333921","https://openalex.org/W1976935047","https://openalex.org/W1981146430","https://openalex.org/W1982376549","https://openalex.org/W1984668528","https://openalex.org/W1984864591","https://openalex.org/W1985212256","https://openalex.org/W1985396526","https://openalex.org/W1988377264","https://openalex.org/W1988517559","https://openalex.org/W1989540103","https://openalex.org/W1990087679","https://openalex.org/W1991841644","https://openalex.org/W1992193255","https://openalex.org/W1993257271","https://openalex.org/W1994206543","https://openalex.org/W1995994950","https://openalex.org/W1996631946","https://openalex.org/W2000686310","https://openalex.org/W2002226709","https://openalex.org/W2004557152","https://openalex.org/W2005066107","https://openalex.org/W2006070968","https://openalex.org/W2006072955","https://openalex.org/W2006652125","https://openalex.org/W2008365039","https://openalex.org/W2008853806","https://openalex.org/W2010182284","https://openalex.org/W2012200567","https://openalex.org/W2012218964","https://openalex.org/W2013320855","https://openalex.org/W2013339424","https://openalex.org/W2013843427","https://openalex.org/W2014899456","https://openalex.org/W2017083140","https://openalex.org/W2018161273","https://openalex.org/W2024058638","https://openalex.org/W2024946243","https://openalex.org/W2025449841","https://openalex.org/W2025999562","https://openalex.org/W2026445365","https://openalex.org/W2028415754","https://openalex.org/W2028695556","https://openalex.org/W2029443499","https://openalex.org/W2029955024","https://openalex.org/W2030542406","https://openalex.org/W2032023073","https://openalex.org/W2032325361","https://openalex.org/W2032440198","https://openalex.org/W2032619610","https://openalex.org/W2033676599","https://openalex.org/W2035901470","https://openalex.org/W2038340941","https://openalex.org/W2039393623","https://openalex.org/W2044213031","https://openalex.org/W2044807445","https://openalex.org/W2046847544","https://openalex.org/W2048639483","https://openalex.org/W2048968441","https://openalex.org/W2049481178","https://openalex.org/W2049855026","https://openalex.org/W2052120376","https://openalex.org/W2052922251","https://openalex.org/W2052960454","https://openalex.org/W2053596672","https://openalex.org/W2054225000","https://openalex.org/W2056405400","https://openalex.org/W2056843562","https://openalex.org/W2058232580","https://openalex.org/W2058645214","https://openalex.org/W2060613642","https://openalex.org/W2062152551","https://openalex.org/W2062808764","https://openalex.org/W2062916647","https://openalex.org/W2064418396","https://openalex.org/W2065691403","https://openalex.org/W2066446604","https://openalex.org/W2072214489","https://openalex.org/W2073248102","https://openalex.org/W2073762862","https://openalex.org/W2074825472","https://openalex.org/W2075511135","https://openalex.org/W2078644939","https://openalex.org/W2079741120","https://openalex.org/W2080255536","https://openalex.org/W2081500116","https://openalex.org/W2082880323","https://openalex.org/W2085911340","https://openalex.org/W2090144945","https://openalex.org/W2090600204","https://openalex.org/W2092637029","https://openalex.org/W2094941511","https://openalex.org/W2094957750","https://openalex.org/W2096882143","https://openalex.org/W2100688137","https://openalex.org/W2100823015","https://openalex.org/W2105548092","https://openalex.org/W2105555459","https://openalex.org/W2106003770","https://openalex.org/W2111853402","https://openalex.org/W2113312001","https://openalex.org/W2114436789","https://openalex.org/W2114802823","https://openalex.org/W2114901715","https://openalex.org/W2120138780","https://openalex.org/W2122189817","https://openalex.org/W2123772979","https://openalex.org/W2124213369","https://openalex.org/W2124306225","https://openalex.org/W2125588561","https://openalex.org/W2126806931","https://openalex.org/W2128035214","https://openalex.org/W2131996349","https://openalex.org/W2132097251","https://openalex.org/W2143112776","https://openalex.org/W2143152659","https://openalex.org/W2146415848","https://openalex.org/W2147314381","https://openalex.org/W2148023511","https://openalex.org/W2148802014","https://openalex.org/W2151078743","https://openalex.org/W2153306295","https://openalex.org/W2154009044","https://openalex.org/W2156523912","https://openalex.org/W2157644555","https://openalex.org/W2160011238","https://openalex.org/W2162851594","https://openalex.org/W2164297374","https://openalex.org/W2165038904","https://openalex.org/W2165360511","https://openalex.org/W2166079697","https://openalex.org/W2167033587","https://openalex.org/W2168612956","https://openalex.org/W2169980814","https://openalex.org/W2171798308","https://openalex.org/W2178029086","https://openalex.org/W2197557695","https://openalex.org/W2219963396","https://openalex.org/W2224021986","https://openalex.org/W2230472805","https://openalex.org/W2239676257","https://openalex.org/W2252312839","https://openalex.org/W2267746238","https://openalex.org/W2277402206","https://openalex.org/W2317164129","https://openalex.org/W2342744565","https://openalex.org/W2395135685","https://openalex.org/W2396229307","https://openalex.org/W2397060233","https://openalex.org/W2411014906","https://openalex.org/W2412211578","https://openalex.org/W2412342807","https://openalex.org/W2414225446","https://openalex.org/W2414468783","https://openalex.org/W2417176285","https://openalex.org/W2432116985","https://openalex.org/W2437575637","https://openalex.org/W2474062789","https://openalex.org/W4241709163"],"related_works":["https://openalex.org/W2724721603","https://openalex.org/W2264431635","https://openalex.org/W2285716872","https://openalex.org/W89764641","https://openalex.org/W257778956","https://openalex.org/W2164297374","https://openalex.org/W7099546904","https://openalex.org/W2017703988","https://openalex.org/W2017518715","https://openalex.org/W2099345045"],"abstract_inverted_index":{"Acute":[0],"alterations":[1,15],"in":[2,16,43,51,101,144],"cell":[3],"volume":[4],"can":[5],"substantively":[6],"modulate":[7,19],"subsequent":[8],"metabolism":[9,21,143],"of":[10,26,36,80,92,133],"substrates.":[11],"However,":[12],"how":[13],"such":[14],"skeletal":[17,44],"muscle":[18,45],"protein":[20,41,86,93,142],"is":[22],"limited.":[23],"The":[24],"purpose":[25],"this":[27],"study":[28],"was":[29,82,109],"to":[30,84],"determine":[31],"the":[32,76],"time":[33],"dependent":[34],"influence":[35],"extracellular":[37,135],"osmotic":[38,136],"stress":[39,137],"on":[40],"turnover":[42],"cells.":[46,146],"L6":[47,145],"cells":[48],"were":[49,99],"incubated":[50],"hyperosmotic":[52],"(HYPER;":[53],"425.3":[54],"\u00b1":[55,60,66],"1.8mmol/kg),":[56],"hypo-osmotic":[57],"(HYPO;":[58],"235.4":[59],"1.0mmol/kg)":[61],"or":[62,73],"control":[63],"(CON;":[64],"333.5":[65],"1.4mmol/kg)":[67],"media":[68],"for":[69,120,126],"4,":[70],"8,":[71],"12,":[72],"24hrs.":[74],"During":[75],"final":[77],"4hrs,":[78],"incorporation":[79],"L-[ring-3,5-3H]-tyrosine":[81],"measured":[83,90],"estimate":[85],"synthesis.":[87],"Western":[88],"blotting":[89],"markers":[91],"synthesis":[94],"and":[95,114],"degradation.":[96],"No":[97],"differences":[98],"observed":[100],"any":[102],"outcomes":[103],"except":[104,119],"p70S6K":[105],"phosphorylation":[106],"whereby":[107],"HYPO":[108],"lower":[110],"(p<0.05)":[111],"than":[112],"CON":[113],"HYPER;":[115],"which":[116],"remained":[117],"similar":[118],"a":[121],"large":[122],"increase":[123],"at":[124],"8hrs":[125],"HYPER.":[127],"These":[128],"findings":[129],"suggest":[130],"that":[131],"regardless":[132],"duration,":[134],"does":[138],"not":[139],"significantly":[140],"affect":[141]},"counts_by_year":[],"updated_date":"2026-10-12T09:40:04.860316","created_date":"2016-06-24T00:00:00"}