{"id":"https://openalex.org/W2189486427","doi":"https://doi.org/10.21608/ejgc.2013.10454","title":"GENETIC TRANSFORMATION OF COMMON BEANS (Phaseolus vulgaris L.) CALLI BY BIOLISTIC GUN AND VIA Agrobacterium tumefaciens","display_name":"GENETIC TRANSFORMATION OF COMMON BEANS (Phaseolus vulgaris L.) CALLI BY BIOLISTIC GUN AND VIA Agrobacterium tumefaciens","publication_year":2013,"publication_date":"2013-01-01","ids":{"openalex":"https://openalex.org/W2189486427","doi":"https://doi.org/10.21608/ejgc.2013.10454","mag":"2189486427"},"language":"en","primary_location":{"id":"doi:10.21608/ejgc.2013.10454","doi":"https://doi.org/10.21608/ejgc.2013.10454","is_oa":true,"landing_page_url":"https://doi.org/10.21608/ejgc.2013.10454","pdf_url":null,"source":{"id":"https://openalex.org/S2764740768","display_name":"Egyptian Journal of Genetics and Cytology","issn_l":"0046-161X","issn":["0046-161X"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310318978","host_organization_name":"The Egyptian Society of Genetics","host_organization_lineage":["https://openalex.org/P4310318978"],"host_organization_lineage_names":["The Egyptian Society of Genetics"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Egyptian Journal of Genetics and Cytology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://doi.org/10.21608/ejgc.2013.10454","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5005776392","display_name":"E. A. Eissa","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"E. EISSA","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5005776392"],"corresponding_institution_ids":[],"apc_list":{"value":750,"currency":"EGP","value_usd":null},"apc_paid":null,"fwci":0.3745,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":{"value":0.63492945,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":93},"biblio":{"volume":"42","issue":"1","first_page":"1","last_page":"20"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12856","display_name":"Transgenic Plants and Applications","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11426","display_name":"Genetically Modified Organisms Research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/common-bean","display_name":"Phaseolus vulgaris","score":1.0},{"id":"https://openalex.org/keywords/genetic-transformation","display_name":"genetic transformation","score":0.9459999799728394},{"id":"https://openalex.org/keywords/particle-bombardment","display_name":"particle bombardment","score":0.7239999771118164},{"id":"https://openalex.org/keywords/agrobacterium-mediated-transformation","display_name":"Agrobacterium-mediated transformation","score":0.8529999852180481},{"id":"https://openalex.org/keywords/callus-induction","display_name":"callus induction","score":0.7739999890327454},{"id":"https://openalex.org/keywords/hypocotyl-explants","display_name":"hypocotyl explants","score":0.7979999780654907},{"id":"https://openalex.org/keywords/transient-expression","display_name":"transient expression","score":0.6380000114440918},{"id":"https://openalex.org/keywords/transgenic-callus","display_name":"transgenic callus","score":0.6209999918937683},{"id":"https://openalex.org/keywords/neomycin-phosphotransferase-ii","display_name":"neomycin phosphotransferase II","score":0.8519999980926514},{"id":"https://openalex.org/keywords/mannitol-1-phosphate-dehydrogenase","display_name":"mannitol-1-phosphate dehydrogenase","score":0.9269999861717224},{"id":"https://openalex.org/keywords/gus-reporter","display_name":"GUS reporter","score":0.859000027179718},{"id":"https://openalex.org/keywords/double-selection","display_name":"double selection","score":0.4230000078678131}],"concepts":[{"id":"https://openalex.org/C2776286235","wikidata":"https://www.wikidata.org/wiki/Q310438","display_name":"Phaseolus","level":2,"score":0.925865113735199},{"id":"https://openalex.org/C2776162087","wikidata":"https://www.wikidata.org/wiki/Q131472","display_name":"Agrobacterium tumefaciens","level":4,"score":0.8590831160545349},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8426926732063293},{"id":"https://openalex.org/C204241405","wikidata":"https://www.wikidata.org/wiki/Q461499","display_name":"Transformation (genetics)","level":3,"score":0.6389472484588623},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.585241436958313},{"id":"https://openalex.org/C2779656422","wikidata":"https://www.wikidata.org/wiki/Q2700446","display_name":"Agrobacterium","level":4,"score":0.4343183636665344},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.24232956767082214},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.22995156049728394}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.21608/ejgc.2013.10454","doi":"https://doi.org/10.21608/ejgc.2013.10454","is_oa":true,"landing_page_url":"https://doi.org/10.21608/ejgc.2013.10454","pdf_url":null,"source":{"id":"https://openalex.org/S2764740768","display_name":"Egyptian Journal of Genetics and Cytology","issn_l":"0046-161X","issn":["0046-161X"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310318978","host_organization_name":"The Egyptian Society of Genetics","host_organization_lineage":["https://openalex.org/P4310318978"],"host_organization_lineage_names":["The Egyptian Society of Genetics"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Egyptian Journal of Genetics and Cytology","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.21608/ejgc.2013.10454","doi":"https://doi.org/10.21608/ejgc.2013.10454","is_oa":true,"landing_page_url":"https://doi.org/10.21608/ejgc.2013.10454","pdf_url":null,"source":{"id":"https://openalex.org/S2764740768","display_name":"Egyptian Journal of Genetics and Cytology","issn_l":"0046-161X","issn":["0046-161X"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310318978","host_organization_name":"The Egyptian Society of Genetics","host_organization_lineage":["https://openalex.org/P4310318978"],"host_organization_lineage_names":["The Egyptian Society of Genetics"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Egyptian Journal of Genetics and Cytology","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Zero hunger","id":"https://metadata.un.org/sdg/2","score":0.9208999872207642}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":42,"referenced_works":["https://openalex.org/W1493797255","https://openalex.org/W1513590677","https://openalex.org/W1514821737","https://openalex.org/W1981101372","https://openalex.org/W1982723846","https://openalex.org/W1983032728","https://openalex.org/W1983420339","https://openalex.org/W1986587865","https://openalex.org/W2017808305","https://openalex.org/W2019461002","https://openalex.org/W2021185056","https://openalex.org/W2024556021","https://openalex.org/W2034475237","https://openalex.org/W2036462208","https://openalex.org/W2042523761","https://openalex.org/W2051863077","https://openalex.org/W2068303746","https://openalex.org/W2071690799","https://openalex.org/W2072402961","https://openalex.org/W2073645741","https://openalex.org/W2077545872","https://openalex.org/W2088081576","https://openalex.org/W2090608609","https://openalex.org/W2095236068","https://openalex.org/W2097289497","https://openalex.org/W2097324472","https://openalex.org/W2110321970","https://openalex.org/W2121181026","https://openalex.org/W2146883202","https://openalex.org/W2168255317","https://openalex.org/W2175442085","https://openalex.org/W2180412090","https://openalex.org/W2182007649","https://openalex.org/W2188403284","https://openalex.org/W2188977276","https://openalex.org/W2315179028","https://openalex.org/W2350684343","https://openalex.org/W2589543966","https://openalex.org/W2991569205","https://openalex.org/W3135186761","https://openalex.org/W4249197373","https://openalex.org/W4285719527"],"related_works":["https://openalex.org/W6998483439","https://openalex.org/W1614453059","https://openalex.org/W2566183896","https://openalex.org/W2131254922","https://openalex.org/W2322967752","https://openalex.org/W2971200208","https://openalex.org/W1601188354","https://openalex.org/W3010201584","https://openalex.org/W2051863077","https://openalex.org/W7007778157"],"abstract_inverted_index":{"Common":[0],"bean":[1,28,268],"is":[2],"one":[3],"of":[4,123,204,242],"the":[5,63,97,101,124,174,177,181,205],"most":[6],"recalcitrant":[7],"species":[8],"for":[9,65,239,258],"in":[10,26,93,173],"vitro":[11],"manipulation":[12],"and":[13,24,38,55,68,83,96,116,121,130,144,176,195,202,209,219,224,235,250],"transformation.":[14],"In":[15],"this":[16],"study":[17],"efficient":[18,232],"genetic":[19],"transformation":[20,237],"methods":[21,238],"were":[22,72,91,142,152,171,217],"developed":[23],"performed":[25],"commercialized":[27],"varieties":[29],"calli":[30,71,94,151,175,184],"by":[31,104,146,221],"utilized":[32],"microparticle":[33],"bombardment":[34],"(direct":[35],"biolistic":[36,105,233],"gun)":[37],"indirect":[39],"A.":[40],"tumefaciens-based":[41],"technique.":[42],"Hypocotyl":[43],"segments":[44],"cultured":[45],"on":[46,75,159,261],"Gamborgs":[47],"medium":[48,77,161],"supplemented":[49],"with":[50,156,186],"1":[51],"mg":[52,57,80,164],"l-l":[53,58,81,86,165],"kinetin":[54],"2":[56],"2,4-dichlorophenoxy":[59],"acetic":[60],"acid":[61],"proved":[62],"best":[64],"callus":[66],"induction":[67],"maintenance.":[69],"Co-transformed":[70],"double":[73,147],"selected":[74,153],"selective":[76,160],"using":[78],"150":[79],"kanamycin":[82,114],"0.8":[84],"mol":[85],"mannitol.":[87],"Transient":[88,168],"genes":[89,136,169,211,245,265],"expressions":[90,170],"obtained":[92,172],"cells":[95,102],"results":[98,178,229],"showed":[99,179],"that":[100,180,251],"shot":[103],"DNA":[106],"delivery":[107],"method":[108,158],"can":[109,188],"be":[110,189,256],"transformed":[111,190],"to":[112,191],"get":[113,192],"resistant":[115,194],"mannitol":[117],"tolerant.":[118],"The":[119,200],"presence":[120,201],"integration":[122,203,241],"neomycin":[125],"phosphotransferase":[126],"II":[127],"(npt":[128],"II)":[129],"osmosis":[131],"protector":[132],"mannitol-1-phosphate":[133],"dehydrogenase":[134],"(mtlD)":[135],"into":[137,212,246,266],"P.":[138,213],"vulgaris":[139,214],"L.":[140,215],"genome":[141,216],"confirmed":[143,218],"verified":[145,220],"selection":[148,223],"tests.":[149,227],"Transgenic":[150],"after":[154],"co-cultivated":[155,185],"Agrobacterium":[157,187],"containing":[162],"5":[163],"phosphinotricin":[166,193,206],"(PPT).":[167],"common":[182,247,267],"beans":[183,248],"\u03b2-glucuronidase":[196,210],"reporter":[197],"positive":[198],"tissues.":[199],"acetyl":[207],"transferase":[208],"marker":[222],"histochemical":[225],"assay":[226],"These":[228],"suggest":[230],"an":[231],"gun,":[234],"Agrobacterium-mediated":[236],"stable":[240],"economically":[243,263],"important":[244,264],"calli,":[249],"these":[252],"transformations":[253],"systems":[254],"could":[255],"useful":[257],"future":[259],"studies":[260],"transferring":[262],"plants.":[269]},"counts_by_year":[{"year":2021,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}