{"id":"https://openalex.org/W2188568216","doi":null,"title":"Parkinson\u2019s Disease Associated Protein \u03b1-Synuclein-E46K Demonstrates Strain and Ploidy-Specific Toxicity in Budding Yeast","display_name":"Parkinson\u2019s Disease Associated Protein \u03b1-Synuclein-E46K Demonstrates Strain and Ploidy-Specific Toxicity in Budding Yeast","publication_year":2008,"publication_date":"2008-04-02","ids":{"openalex":"https://openalex.org/W2188568216","mag":"2188568216"},"language":"en","primary_location":{"id":"pmh:oai:publications.lakeforest.edu:eukaryon-1130","doi":null,"is_oa":false,"landing_page_url":"https://publications.lakeforest.edu/eukaryon/vol4/iss1/41","pdf_url":null,"source":{"id":"https://openalex.org/S4306510078","display_name":"Eukaryon","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Eukaryon","raw_type":"text"},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"White, Michael","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"White, Michael","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10085","display_name":"Parkinson's Disease Mechanisms and Treatments","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2728","display_name":"Clinical Neurology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10085","display_name":"Parkinson's Disease Mechanisms and Treatments","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2728","display_name":"Clinical Neurology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10086","display_name":"Alzheimer's disease research and treatments","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13991","display_name":"Nuclear Receptors and Signaling","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/parkinson-s-disease","display_name":"Parkinson's disease","score":0.9190000295639038},{"id":"https://openalex.org/keywords/alpha-synuclein","display_name":"alpha-synuclein","score":0.9879999756813049},{"id":"https://openalex.org/keywords/e46k-mutation","display_name":"E46K mutation","score":0.9860000014305115},{"id":"https://openalex.org/keywords/familial-parkinson-s-disease","display_name":"familial Parkinson's disease","score":0.8579999804496765},{"id":"https://openalex.org/keywords/lewy-bodies","display_name":"Lewy bodies","score":0.640999972820282},{"id":"https://openalex.org/keywords/protein-misfolding","display_name":"protein misfolding","score":0.8180000185966492},{"id":"https://openalex.org/keywords/diploid-yeast","display_name":"diploid yeast","score":0.6579999923706055},{"id":"https://openalex.org/keywords/haploid-yeast","display_name":"haploid yeast","score":0.8130000233650208},{"id":"https://openalex.org/keywords/membrane-localization","display_name":"membrane localization","score":0.7590000033378601},{"id":"https://openalex.org/keywords/a30p-mutation","display_name":"A30P mutation","score":0.6890000104904175},{"id":"https://openalex.org/keywords/a53t-mutation","display_name":"A53T mutation","score":0.996999979019165},{"id":"https://openalex.org/keywords/neurodegeneration","display_name":"neurodegeneration","score":0.49900001287460327},{"id":"https://openalex.org/keywords/saccharomyces-cerevisiae","display_name":"Saccharomyces cerevisiae","score":0.8669999837875366}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7282453775405884},{"id":"https://openalex.org/C143191323","wikidata":"https://www.wikidata.org/wiki/Q118406","display_name":"Ploidy","level":3,"score":0.526434063911438},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.5165725946426392},{"id":"https://openalex.org/C188082640","wikidata":"https://www.wikidata.org/wiki/Q1780899","display_name":"Complementation","level":4,"score":0.4888274073600769},{"id":"https://openalex.org/C46909000","wikidata":"https://www.wikidata.org/wiki/Q3604177","display_name":"Mating of yeast","level":4,"score":0.47993022203445435},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.46979793906211853},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.4277650713920593},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.22895368933677673}],"mesh":[],"locations_count":1,"locations":[{"id":"pmh:oai:publications.lakeforest.edu:eukaryon-1130","doi":null,"is_oa":false,"landing_page_url":"https://publications.lakeforest.edu/eukaryon/vol4/iss1/41","pdf_url":null,"source":{"id":"https://openalex.org/S4306510078","display_name":"Eukaryon","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Eukaryon","raw_type":"text"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.8371999859809875,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":41,"referenced_works":["https://openalex.org/W1499858465","https://openalex.org/W1521854746","https://openalex.org/W1618846043","https://openalex.org/W1637321639","https://openalex.org/W1877873625","https://openalex.org/W1965778124","https://openalex.org/W1967233561","https://openalex.org/W1971389338","https://openalex.org/W1973818136","https://openalex.org/W1986625112","https://openalex.org/W1990726987","https://openalex.org/W1991241547","https://openalex.org/W1997597731","https://openalex.org/W2005379695","https://openalex.org/W2009255416","https://openalex.org/W2009556855","https://openalex.org/W2018633688","https://openalex.org/W2023245921","https://openalex.org/W2025922492","https://openalex.org/W2033847972","https://openalex.org/W2036423396","https://openalex.org/W2040706104","https://openalex.org/W2050786534","https://openalex.org/W2064132534","https://openalex.org/W2067137072","https://openalex.org/W2068288454","https://openalex.org/W2087389265","https://openalex.org/W2088546346","https://openalex.org/W2088694143","https://openalex.org/W2089386161","https://openalex.org/W2091214947","https://openalex.org/W2091265794","https://openalex.org/W2097965815","https://openalex.org/W2119676422","https://openalex.org/W2144435948","https://openalex.org/W2149320847","https://openalex.org/W2150656456","https://openalex.org/W2158437989","https://openalex.org/W2166789153","https://openalex.org/W2313186731","https://openalex.org/W2768578200"],"related_works":["https://openalex.org/W2151452488","https://openalex.org/W7095946782","https://openalex.org/W2073773191","https://openalex.org/W2290446142","https://openalex.org/W4225410860","https://openalex.org/W2018627300","https://openalex.org/W2626361698","https://openalex.org/W6982641038","https://openalex.org/W7221517202","https://openalex.org/W2125627689"],"abstract_inverted_index":{"Parkinson's":[0],"disease":[1],"(PD)":[2],"is":[3,51,78],"a":[4,101,181],"fatal":[5],"neurodegenerative":[6],"disorder":[7],"that":[8,91,105,298],"affects":[9],"1":[10],"in":[11,73,100,107,127,169,227,273,315,323],"100":[12],"individuals":[13],"over":[14],"the":[15,27,31,33,52,66,74,81,84,92,96,118,230,233,237,253,258,261,274,309],"age":[16],"of":[17,30,36,48,57,83,98,120,163,189,229,236,260,308],"60.":[18],"PD":[19,58],"results":[20,106],"from":[21,65],"dopaminergic":[22],"neuronal":[23,324],"atrophy":[24],"localized":[25],"within":[26,41],"substantia":[28,42],"nigra":[29],"midbrain;":[32],"pathology":[34],"consists":[35],"fibrillar":[37],"inclusions,":[38],"Lewy":[39,49],"Bodies,":[40],"nigral":[43],"neurons.":[44],"The":[45],"principal":[46],"component":[47],"Bodies":[50],"protein":[53,155],"alpha-synuclein.":[54],"Though":[55],"90-95%":[56],"cases":[59],"are":[60],"sporadic,":[61],"familial":[62,136,158,327],"forms":[63],"result":[64],"missense":[67],"mutations":[68,149],"A30P,":[69],"A53T,":[70],"or":[71],"E46K":[72,87,93,126,141,194,204,223,244,266,268,291,313],"alpha-synuclein":[75,99,109,299],"gene.":[76],"Little":[77],"known":[79],"about":[80],"properties":[82],"recently":[85],"discovered":[86],"mutation.":[88],"We":[89],"hypothesized":[90],"mutation":[94],"alters":[95,112],"conformation":[97],"potentially":[102],"toxic":[103,238,263,272],"manner":[104],"increased":[108],"misfolding":[110],"and":[111,139,147,150,160,173,180,186,211,267,281,326],"its":[113,321],"plasma":[114],"membrane":[115,300],"localization.":[116],"Therefore,":[117,240],"purpose":[119],"this":[121,289],"thesis":[122],"was":[123,206,225,246,302],"to":[124,195,201,241],"characterize":[125],"budding":[128,176],"yeast":[129,177,316],"(Saccharomyces":[130],"cerevisiae).":[131],"To":[132],"further":[133],"compare":[134],"all":[135],"mutants,":[137,159],"double":[138],"triple":[140],"isoforms":[142],"were":[143,167,214,250,270],"synthesized":[144],"with":[145,152,304],"A30P":[146],"A53T":[148],"tagged":[151],"green":[153],"fluorescent":[154],"(GFP).":[156],"All":[157],"control":[161],"GFP":[162,293],"parent":[164],"vector":[165],"pYES2":[166],"expressed":[168],"several":[170,217],"naturally":[171],"occurring":[172],"commonly":[174],"studied":[175],"haploid":[178,191,219],"strains":[179,231,285],"diploid":[182,254],"strain.":[183,239],"Growth":[184],"curves":[185],"viability":[187,212],"assays":[188,213],"one":[190],"strain":[192,207,255,280],"revealed":[193,297],"be":[196],"toxic.":[197],"Consequently,":[198],"we":[199],"wanted":[200],"determind":[202],"if":[203,243],"toxicity":[205,224,245,314,325],"specific.":[208],"Identical":[209],"growth":[210],"performed":[215,251],"on":[216,252],"other":[218],"strains.":[220,264],"Interestingly,":[221],"no":[222],"observed":[226],"any":[228],"except":[232],"opposite-mating":[234],"type":[235],"test":[242],"ploidy-specific,":[247],"these":[248],"analyses":[249],"formed":[256],"by":[257],"mating":[259],"two":[262],"Unexpectedly,":[265],"variations":[269],"not":[271],"nearly":[275],"genetically":[276],"idential":[277],"diploid.":[278],"Thus,":[279],"ploidy":[282],"differences":[283],"between":[284],"must":[286],"account":[287],"for":[288,312],"selective":[290],"toxicity.":[292,305],"fluorescence":[294],"live-cell":[295],"microscopy":[296],"localization":[301],"correlated":[303],"Further":[306],"study":[307],"underlying":[310],"mechanisms":[311],"may":[317],"provide":[318],"insight":[319],"into":[320],"role":[322],"PD.":[328]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2025-10-10T00:00:00"}
