{"id":"https://openalex.org/W2188550374","doi":null,"title":"Characterisation of cytosolic prion protein-mediated putative cytotoxicity in neuronal cell lines","display_name":"Characterisation of cytosolic prion protein-mediated putative cytotoxicity in neuronal cell lines","publication_year":2007,"publication_date":"2007-02-20","ids":{"openalex":"https://openalex.org/W2188550374","mag":"2188550374"},"language":"en","primary_location":{"id":"pmh:oai:publikationen.ub.uni-frankfurt.de:1661","doi":null,"is_oa":true,"landing_page_url":"http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/1661","pdf_url":"http://publikationen.ub.uni-frankfurt.de/files/1661/MehlhaseJana.pdf","source":{"id":"https://openalex.org/S4306400432","display_name":"Publication Server of Goethe University Frankfurt am Main (Goethe University Frankfurt)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I114090438","host_organization_name":"Goethe University Frankfurt","host_organization_lineage":["https://openalex.org/I114090438"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"doctoralthesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://publikationen.ub.uni-frankfurt.de/files/1661/MehlhaseJana.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5059785764","display_name":"Jana Mehlhase","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Mehlhase, Jana","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5059785764"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11335","display_name":"Prion Diseases and Protein Misfolding","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11335","display_name":"Prion Diseases and Protein Misfolding","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10086","display_name":"Alzheimer's disease research and treatments","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10855","display_name":"Amyotrophic Lateral Sclerosis Research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2728","display_name":"Clinical Neurology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/prion-disease","display_name":"prion disease","score":0.7400000095367432},{"id":"https://openalex.org/keywords/neurotoxicity","display_name":"neurotoxicity","score":0.8479999899864197},{"id":"https://openalex.org/keywords/proteasomal-degradation","display_name":"proteasomal degradation","score":0.7360000014305115},{"id":"https://openalex.org/keywords/hsc70","display_name":"Hsc70","score":0.6959999799728394},{"id":"https://openalex.org/keywords/endosomal-trafficking","display_name":"endosomal trafficking","score":0.4880000054836273},{"id":"https://openalex.org/keywords/er-associated-degradation","display_name":"ER-associated degradation","score":0.5070000290870667},{"id":"https://openalex.org/keywords/neuro-2a-cells","display_name":"N2a cells","score":0.8109999895095825},{"id":"https://openalex.org/keywords/prion-aggregation","display_name":"prion aggregation","score":0.5920000076293945},{"id":"https://openalex.org/keywords/neurodegeneration","display_name":"neurodegeneration","score":0.5950000286102295},{"id":"https://openalex.org/keywords/molecular-chaperones","display_name":"molecular chaperones","score":0.47699999809265137}],"concepts":[{"id":"https://openalex.org/C109316439","wikidata":"https://www.wikidata.org/wiki/Q246181","display_name":"Cytotoxicity","level":3,"score":0.7452405095100403},{"id":"https://openalex.org/C98539663","wikidata":"https://www.wikidata.org/wiki/Q220599","display_name":"Cytosol","level":3,"score":0.6798439025878906},{"id":"https://openalex.org/C3019804061","wikidata":"https://www.wikidata.org/wiki/Q14881255","display_name":"Prion protein","level":3,"score":0.5076736211776733},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.5027022361755371},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.48185670375823975},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4708178639411926},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.45536014437675476},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.37436532974243164},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3224376440048218},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2672533392906189},{"id":"https://openalex.org/C2779134260","wikidata":"https://www.wikidata.org/wiki/Q12136","display_name":"Disease","level":2,"score":0.14170411229133606},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.12269338965415955},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.12057721614837646},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.0},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"pmh:oai:publikationen.ub.uni-frankfurt.de:1661","doi":null,"is_oa":true,"landing_page_url":"http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/1661","pdf_url":"http://publikationen.ub.uni-frankfurt.de/files/1661/MehlhaseJana.pdf","source":{"id":"https://openalex.org/S4306400432","display_name":"Publication Server of Goethe University Frankfurt am Main (Goethe University Frankfurt)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I114090438","host_organization_name":"Goethe University Frankfurt","host_organization_lineage":["https://openalex.org/I114090438"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"doctoralthesis"}],"best_oa_location":{"id":"pmh:oai:publikationen.ub.uni-frankfurt.de:1661","doi":null,"is_oa":true,"landing_page_url":"http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/1661","pdf_url":"http://publikationen.ub.uni-frankfurt.de/files/1661/MehlhaseJana.pdf","source":{"id":"https://openalex.org/S4306400432","display_name":"Publication Server of Goethe University Frankfurt am Main (Goethe University Frankfurt)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I114090438","host_organization_name":"Goethe University Frankfurt","host_organization_lineage":["https://openalex.org/I114090438"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"doctoralthesis"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.9537000060081482}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2188550374.pdf","grobid_xml":"https://content.openalex.org/works/W2188550374.grobid-xml"},"referenced_works_count":230,"referenced_works":["https://openalex.org/W11395008","https://openalex.org/W11571277","https://openalex.org/W36603652","https://openalex.org/W63859567","https://openalex.org/W136234913","https://openalex.org/W1501494207","https://openalex.org/W1502562397","https://openalex.org/W1517182987","https://openalex.org/W1517480469","https://openalex.org/W1518703223","https://openalex.org/W1525316969","https://openalex.org/W1532356924","https://openalex.org/W1541950149","https://openalex.org/W1550540174","https://openalex.org/W1554099072","https://openalex.org/W1554445829","https://openalex.org/W1558608994","https://openalex.org/W1559478955","https://openalex.org/W1575128625","https://openalex.org/W1580230845","https://openalex.org/W1591693496","https://openalex.org/W1592486646","https://openalex.org/W1601881612","https://openalex.org/W1614056212","https://openalex.org/W1704732331","https://openalex.org/W1815844802","https://openalex.org/W1901873593","https://openalex.org/W1918251645","https://openalex.org/W1964207820","https://openalex.org/W1964376084","https://openalex.org/W1965011234","https://openalex.org/W1967322347","https://openalex.org/W1969192163","https://openalex.org/W1969523109","https://openalex.org/W1969680461","https://openalex.org/W1969758868","https://openalex.org/W1969875668","https://openalex.org/W1972409470","https://openalex.org/W1972647829","https://openalex.org/W1972760023","https://openalex.org/W1972980743","https://openalex.org/W1973868747","https://openalex.org/W1974384579","https://openalex.org/W1974443289","https://openalex.org/W1975822812","https://openalex.org/W1976571465","https://openalex.org/W1978999653","https://openalex.org/W1981250435","https://openalex.org/W1981678822","https://openalex.org/W1982576150","https://openalex.org/W1984191886","https://openalex.org/W1984625499","https://openalex.org/W1984944464","https://openalex.org/W1985630952","https://openalex.org/W1987975569","https://openalex.org/W1988607142","https://openalex.org/W1989081602","https://openalex.org/W1989400359","https://openalex.org/W1991624956","https://openalex.org/W1992084965","https://openalex.org/W1992380356","https://openalex.org/W1992603978","https://openalex.org/W1992659255","https://openalex.org/W1993198483","https://openalex.org/W1994142447","https://openalex.org/W1994583387","https://openalex.org/W1997725693","https://openalex.org/W1998038512","https://openalex.org/W1998974062","https://openalex.org/W2000036908","https://openalex.org/W2000504232","https://openalex.org/W2000819625","https://openalex.org/W2000966016","https://openalex.org/W2001002729","https://openalex.org/W2001464510","https://openalex.org/W2002282211","https://openalex.org/W2006104618","https://openalex.org/W2008004502","https://openalex.org/W2010238780","https://openalex.org/W2010497554","https://openalex.org/W2011824677","https://openalex.org/W2013310295","https://openalex.org/W2013944211","https://openalex.org/W2014194922","https://openalex.org/W2014388432","https://openalex.org/W2014742609","https://openalex.org/W2015233583","https://openalex.org/W2015905498","https://openalex.org/W2017014906","https://openalex.org/W2017267117","https://openalex.org/W2017886031","https://openalex.org/W2018739152","https://openalex.org/W2021489997","https://openalex.org/W2021517653","https://openalex.org/W2022680168","https://openalex.org/W2022993326","https://openalex.org/W2023462803","https://openalex.org/W2023680254","https://openalex.org/W2024954984","https://openalex.org/W2025270890","https://openalex.org/W2026314272","https://openalex.org/W2027262540","https://openalex.org/W2028295349","https://openalex.org/W2028413113","https://openalex.org/W2028476667","https://openalex.org/W2029265132","https://openalex.org/W2031445552","https://openalex.org/W2031567073","https://openalex.org/W2031740592","https://openalex.org/W2034359525","https://openalex.org/W2036645353","https://openalex.org/W2036887368","https://openalex.org/W2037426803","https://openalex.org/W2037491812","https://openalex.org/W2039456360","https://openalex.org/W2040504790","https://openalex.org/W2041867303","https://openalex.org/W2042978244","https://openalex.org/W2043387126","https://openalex.org/W2043526072","https://openalex.org/W2044004977","https://openalex.org/W2046241482","https://openalex.org/W2047728873","https://openalex.org/W2048512065","https://openalex.org/W2049125266","https://openalex.org/W2049510318","https://openalex.org/W2049596606","https://openalex.org/W2049627175","https://openalex.org/W2049713657","https://openalex.org/W2049987625","https://openalex.org/W2050331820","https://openalex.org/W2050717506","https://openalex.org/W2053222717","https://openalex.org/W2053636966","https://openalex.org/W2055684980","https://openalex.org/W2057293794","https://openalex.org/W2057491765","https://openalex.org/W2058527659","https://openalex.org/W2058569537","https://openalex.org/W2059891559","https://openalex.org/W2062453540","https://openalex.org/W2063300419","https://openalex.org/W2063880942","https://openalex.org/W2064303517","https://openalex.org/W2068424399","https://openalex.org/W2068947562","https://openalex.org/W2069148762","https://openalex.org/W2069227225","https://openalex.org/W2070364123","https://openalex.org/W2071718698","https://openalex.org/W2072057268","https://openalex.org/W2072718213","https://openalex.org/W2073057312","https://openalex.org/W2073991273","https://openalex.org/W2076020654","https://openalex.org/W2076072586","https://openalex.org/W2077281287","https://openalex.org/W2077404304","https://openalex.org/W2077672823","https://openalex.org/W2078117880","https://openalex.org/W2080514032","https://openalex.org/W2081333099","https://openalex.org/W2081395125","https://openalex.org/W2081852339","https://openalex.org/W2084287054","https://openalex.org/W2084833442","https://openalex.org/W2089199332","https://openalex.org/W2089507595","https://openalex.org/W2090084666","https://openalex.org/W2090394275","https://openalex.org/W2090429649","https://openalex.org/W2090501101","https://openalex.org/W2093468942","https://openalex.org/W2094478098","https://openalex.org/W2095402560","https://openalex.org/W2095619100","https://openalex.org/W2096510701","https://openalex.org/W2096966529","https://openalex.org/W2098561880","https://openalex.org/W2100837269","https://openalex.org/W2101908803","https://openalex.org/W2103613160","https://openalex.org/W2106200048","https://openalex.org/W2106369820","https://openalex.org/W2106494704","https://openalex.org/W2106874569","https://openalex.org/W2107767510","https://openalex.org/W2109401365","https://openalex.org/W2111278119","https://openalex.org/W2114277918","https://openalex.org/W2116299484","https://openalex.org/W2116366663","https://openalex.org/W2118342916","https://openalex.org/W2118365019","https://openalex.org/W2118510702","https://openalex.org/W2119616506","https://openalex.org/W2121583082","https://openalex.org/W2122511788","https://openalex.org/W2122708921","https://openalex.org/W2123465568","https://openalex.org/W2127540247","https://openalex.org/W2129159114","https://openalex.org/W2131020698","https://openalex.org/W2133301860","https://openalex.org/W2135378430","https://openalex.org/W2136681370","https://openalex.org/W2139174538","https://openalex.org/W2142094544","https://openalex.org/W2143795630","https://openalex.org/W2148396143","https://openalex.org/W2149348969","https://openalex.org/W2152693222","https://openalex.org/W2157234645","https://openalex.org/W2157295566","https://openalex.org/W2158707059","https://openalex.org/W2161396884","https://openalex.org/W2161844942","https://openalex.org/W2162766104","https://openalex.org/W2163981524","https://openalex.org/W2164599456","https://openalex.org/W2167503783","https://openalex.org/W2168129906","https://openalex.org/W2168620413","https://openalex.org/W2169043398","https://openalex.org/W2169896854","https://openalex.org/W2171825278","https://openalex.org/W2218155072","https://openalex.org/W2253907259","https://openalex.org/W2304202484","https://openalex.org/W2416524527"],"related_works":["https://openalex.org/W1982576150","https://openalex.org/W2049125266","https://openalex.org/W2127486078","https://openalex.org/W1978220525","https://openalex.org/W2079724400","https://openalex.org/W2077404304","https://openalex.org/W2048099178","https://openalex.org/W202230252","https://openalex.org/W2123465568","https://openalex.org/W2033268132"],"abstract_inverted_index":{"Prion":[0],"diseases":[1],"are":[2,16,34],"a":[3,11,123,142,256],"complex":[4],"group":[5],"of":[6,31,54,63,83,90,129,139,145,178,190,209,262,284],"fatal":[7],"neurodegenerative":[8],"disorders":[9],"with":[10,185,234],"broad":[12],"host":[13],"spectrum,":[14],"which":[15],"characterised":[17],"by":[18,51,217],"strong":[19],"neuronal":[20,45],"cell":[21,46,191],"loss,":[22],"spongiform":[23],"vacuolation":[24],"and":[25,102,232,266],"astrocytic":[26],"proliferation.":[27],"The":[28,158],"molecular":[29,137],"mechanisms":[30,138],"prion-mediated":[32],"neurodegeneration":[33],"not":[35,167,215],"yet":[36],"fully":[37],"understood.":[38],"Recently,":[39],"it":[40,247],"has":[41],"been":[42,116],"proposed":[43,249],"that":[44,250],"death":[47],"might":[48],"be":[49,97],"triggered":[50],"cytosolic":[52,91,109,150,236],"accumulation":[53,283],"misfolded":[55],"cellular":[56],"prion":[57,130],"protein":[58],"(PrPC)":[59],"due":[60],"to":[61,96,134,171,269,278],"impairment":[62],"proteasomal":[64,219],"degradation.":[65],"Cytosolic":[66,118],"PrPC":[67,84],"could":[68],"result":[69],"from":[70],"either":[71],"retro-translocation":[72],"via":[73],"the":[74,86,127,136,188,198,207,218,235,251,259,287],"endoplasmatic":[75],"reticulum-associated":[76],"degradation":[77,189,205],"system":[78],"(ERAD)":[79],"or":[80],"abortive":[81],"translocation":[82],"into":[85],"ER.":[87],"Indeed,":[88],"expression":[89,165],"PrP":[92,151],"(Cy-PrP)":[93,152],"was":[94,153,201,248],"shown":[95],"neurotoxic":[98],"both":[99],"in":[100,103,112,126,155,174,224,239,286],"vivo":[101],"vitro.":[104],"However,":[105],"contradicting":[106],"results":[107,160],"on":[108],"PrP-mediated":[110],"neurotoxicity":[111],"cultured":[113],"cells":[114,147,176,280],"have":[115],"reported.":[117],"PrP\u2013mediated":[119],"cytotoxicity":[120,173],"may":[121,275],"play":[122],"central":[124],"role":[125],"pathogenesis":[128],"diseases.":[131],"In":[132,195],"order":[133],"investigate":[135],"this":[140,156,196],"process,":[141,197],"detailed":[143],"analysis":[144],"N2a":[146,175,279],"conditionally":[148],"expressing":[149],"performed":[154],"study.":[157],"following":[159],"were":[161],"obtained:":[162],"First,":[163],"Cy-PrP":[164,182,204,222,264,285],"is":[166,183,214],"per":[168],"se":[169],"sufficient":[170],"trigger":[172],"independently":[177],"proteasome":[179,200],"inhibition.":[180],"Second,":[181],"degraded":[184],"kinetics":[186],"resembling":[187],"membrane-anchored":[192],"full-length":[193],"PM-PrP.":[194],"20/26S":[199],"responsible":[202],"for":[203,258],"while":[206],"proteolysis":[208],"matured":[210],"full":[211],"length":[212],"PM-PrP":[213],"affected":[216],"system.":[220],"Third,":[221],"accumulates":[223],"fine":[225],"foci":[226],"when":[227],"expressed":[228],"at":[229],"high":[230],"levels":[231],"co-localises":[233],"chaperone":[237,252],"Hsc70":[238,253],"EEA-1":[240],"positive":[241],"endocytic":[242],"vesicles.":[243,271],"From":[244],"these":[245],"data":[246],"acts":[254],"as":[255],"regulator":[257],"controlled":[260],"formation":[261],"amorphous":[263],"aggregates":[265],"their":[267],"transport":[268],"endosomal":[270],"This":[272],"Hsc70-dependent":[273],"mechanism":[274],"confer":[276],"protection":[277],"against":[281],"toxic":[282],"cytosol.":[288]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2025-10-10T00:00:00"}