{"id":"https://openalex.org/W2188396331","doi":null,"title":"Functional Mapping of Mu Transposition Machinery : MuA Protein Modification and Engineering for Hyperactivity","display_name":"Functional Mapping of Mu Transposition Machinery : MuA Protein Modification and Engineering for Hyperactivity","publication_year":2013,"publication_date":"2013-05-17","ids":{"openalex":"https://openalex.org/W2188396331","mag":"2188396331"},"language":"en","primary_location":{"id":"pmh:oai:helda.helsinki.fi:10138/38927","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10138/38927","pdf_url":"http://hdl.handle.net/10138/38927","source":{"id":"https://openalex.org/S4210213322","display_name":"Ty\u00f6v\u00e4entutkimus Vuosikirja","issn_l":"0784-1272","issn":["0784-1272","1459-7780"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["ddh","jufo-1"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"doctoralThesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://hdl.handle.net/10138/38927","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5054646166","display_name":"Tiina S. Rasila","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Tiina Rasila","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5054646166"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.6664999723434448,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.6664999723434448,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.3075999915599823,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.0066999997943639755,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bacteriophage-mu","display_name":"bacteriophage Mu","score":0.8550000190734863},{"id":"https://openalex.org/keywords/transpososome","display_name":"transpososome","score":0.8790000081062317},{"id":"https://openalex.org/keywords/structure-function-relationship","display_name":"structure-function relationship","score":0.593999981880188},{"id":"https://openalex.org/keywords/random-mutagenesis","display_name":"random mutagenesis","score":0.8059999942779541},{"id":"https://openalex.org/keywords/protein-modification-sites","display_name":"protein modification sites","score":0.7310000061988831},{"id":"https://openalex.org/keywords/hiv-integrase","display_name":"HIV integrase","score":0.7839999794960022},{"id":"https://openalex.org/keywords/v-d-j-recombination","display_name":"V(D)J recombination","score":0.8999999761581421}],"concepts":[{"id":"https://openalex.org/C12455157","wikidata":"https://www.wikidata.org/wiki/Q7835331","display_name":"Transposition (logic)","level":2,"score":0.6061809062957764},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.3505566716194153},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.3239249587059021},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.18655672669410706}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:helda.helsinki.fi:10138/38927","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10138/38927","pdf_url":"http://hdl.handle.net/10138/38927","source":{"id":"https://openalex.org/S4210213322","display_name":"Ty\u00f6v\u00e4entutkimus Vuosikirja","issn_l":"0784-1272","issn":["0784-1272","1459-7780"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["ddh","jufo-1"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"doctoralThesis"},{"id":"mag:2188396331","doi":null,"is_oa":false,"landing_page_url":"https://helda.helsinki.fi/bitstream/handle/10138/38927/rasila_dissertation.pdf?sequence=1","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:helda.helsinki.fi:10138/38927","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10138/38927","pdf_url":"http://hdl.handle.net/10138/38927","source":{"id":"https://openalex.org/S4210213322","display_name":"Ty\u00f6v\u00e4entutkimus Vuosikirja","issn_l":"0784-1272","issn":["0784-1272","1459-7780"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["ddh","jufo-1"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"doctoralThesis"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320310086","display_name":"Helsingin Yliopisto","ror":"https://ror.org/040af2s02"},{"id":"https://openalex.org/F4320321726","display_name":"Suomen Kulttuurirahasto","ror":"https://ror.org/027xav248"},{"id":"https://openalex.org/F4320322861","display_name":"Emil Aaltosen S\u00e4\u00e4ti\u00f6","ror":"https://ror.org/005rt3g54"},{"id":"https://openalex.org/F4320323313","display_name":"Turun Yliopisto","ror":"https://ror.org/05vghhr25"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2188396331.pdf","grobid_xml":"https://content.openalex.org/works/W2188396331.grobid-xml"},"referenced_works_count":315,"referenced_works":["https://openalex.org/W5758259","https://openalex.org/W142445110","https://openalex.org/W194348575","https://openalex.org/W233486025","https://openalex.org/W263204766","https://openalex.org/W318942828","https://openalex.org/W759901999","https://openalex.org/W801609692","https://openalex.org/W1484058837","https://openalex.org/W1498284589","https://openalex.org/W1501132333","https://openalex.org/W1519479476","https://openalex.org/W1528861111","https://openalex.org/W1532359037","https://openalex.org/W1539795437","https://openalex.org/W1554685521","https://openalex.org/W1563552185","https://openalex.org/W1570184892","https://openalex.org/W1574702239","https://openalex.org/W1575524688","https://openalex.org/W1581746888","https://openalex.org/W1588066949","https://openalex.org/W1591378180","https://openalex.org/W1595914273","https://openalex.org/W1603242590","https://openalex.org/W1612971777","https://openalex.org/W1617957008","https://openalex.org/W1645874374","https://openalex.org/W1652529314","https://openalex.org/W1705448411","https://openalex.org/W1738249403","https://openalex.org/W1804262888","https://openalex.org/W1860484030","https://openalex.org/W1903286920","https://openalex.org/W1931692559","https://openalex.org/W1948490957","https://openalex.org/W1953270925","https://openalex.org/W1964588419","https://openalex.org/W1964958456","https://openalex.org/W1966765851","https://openalex.org/W1967060042","https://openalex.org/W1967864930","https://openalex.org/W1968020427","https://openalex.org/W1969113289","https://openalex.org/W1970062083","https://openalex.org/W1971632262","https://openalex.org/W1972416948","https://openalex.org/W1972669919","https://openalex.org/W1972812440","https://openalex.org/W1972824210","https://openalex.org/W1972929796","https://openalex.org/W1973039618","https://openalex.org/W1973410399","https://openalex.org/W1974451402","https://openalex.org/W1974919845","https://openalex.org/W1975890187","https://openalex.org/W1978375191","https://openalex.org/W1979647307","https://openalex.org/W1979822004","https://openalex.org/W1979839738","https://openalex.org/W1980137879","https://openalex.org/W1980829286","https://openalex.org/W1981036200","https://openalex.org/W1981266474","https://openalex.org/W1984336657","https://openalex.org/W1984409688","https://openalex.org/W1985220915","https://openalex.org/W1985611354","https://openalex.org/W1985661898","https://openalex.org/W1985954201","https://openalex.org/W1986118467","https://openalex.org/W1986407852","https://openalex.org/W1986592873","https://openalex.org/W1987752798","https://openalex.org/W1988037036","https://openalex.org/W1988913719","https://openalex.org/W1990644693","https://openalex.org/W1991945355","https://openalex.org/W1992644222","https://openalex.org/W1994777317","https://openalex.org/W1997015356","https://openalex.org/W1997327174","https://openalex.org/W1999078569","https://openalex.org/W1999196532","https://openalex.org/W1999510588","https://openalex.org/W2001109802","https://openalex.org/W2001650493","https://openalex.org/W2002149565","https://openalex.org/W2002500132","https://openalex.org/W2002876883","https://openalex.org/W2003390978","https://openalex.org/W2003392521","https://openalex.org/W2005270270","https://openalex.org/W2006444198","https://openalex.org/W2006572311","https://openalex.org/W2007332550","https://openalex.org/W2007605657","https://openalex.org/W2009448271","https://openalex.org/W2009780091","https://openalex.org/W2011513747","https://openalex.org/W2011624846","https://openalex.org/W2011804687","https://openalex.org/W2011814921","https://openalex.org/W2012563603","https://openalex.org/W2012602999","https://openalex.org/W2012819319","https://openalex.org/W2013558642","https://openalex.org/W2013745116","https://openalex.org/W2013755414","https://openalex.org/W2014732955","https://openalex.org/W2015393318","https://openalex.org/W2015691943","https://openalex.org/W2016189415","https://openalex.org/W2017738521","https://openalex.org/W2018218643","https://openalex.org/W2018388983","https://openalex.org/W2018450306","https://openalex.org/W2020038980","https://openalex.org/W2020663725","https://openalex.org/W2021225519","https://openalex.org/W2021933858","https://openalex.org/W2022940274","https://openalex.org/W2025344323","https://openalex.org/W2026862785","https://openalex.org/W2026952154","https://openalex.org/W2028094900","https://openalex.org/W2028182962","https://openalex.org/W2028966629","https://openalex.org/W2029130767","https://openalex.org/W2029162247","https://openalex.org/W2029357243","https://openalex.org/W2030150331","https://openalex.org/W2030735657","https://openalex.org/W2031250303","https://openalex.org/W2031673350","https://openalex.org/W2031977279","https://openalex.org/W2032412801","https://openalex.org/W2032542798","https://openalex.org/W2033134651","https://openalex.org/W2034232197","https://openalex.org/W2034741116","https://openalex.org/W2036223507","https://openalex.org/W2036994672","https://openalex.org/W2037671516","https://openalex.org/W2038264207","https://openalex.org/W2040064091","https://openalex.org/W2040865473","https://openalex.org/W2042086471","https://openalex.org/W2043468227","https://openalex.org/W2044251005","https://openalex.org/W2044418786","https://openalex.org/W2045553263","https://openalex.org/W2045680907","https://openalex.org/W2047543139","https://openalex.org/W2047736683","https://openalex.org/W2048017191","https://openalex.org/W2048604274","https://openalex.org/W2048843107","https://openalex.org/W2049577511","https://openalex.org/W2050228332","https://openalex.org/W2050854523","https://openalex.org/W2052453497","https://openalex.org/W2053066561","https://openalex.org/W2054458141","https://openalex.org/W2054622780","https://openalex.org/W2054875735","https://openalex.org/W2055475784","https://openalex.org/W2055793859","https://openalex.org/W2056019385","https://openalex.org/W2056183396","https://openalex.org/W2057173230","https://openalex.org/W2058697565","https://openalex.org/W2059992267","https://openalex.org/W2060012575","https://openalex.org/W2060425871","https://openalex.org/W2060851629","https://openalex.org/W2061081201","https://openalex.org/W2062783103","https://openalex.org/W2063337115","https://openalex.org/W2063527435","https://openalex.org/W2064092755","https://openalex.org/W2067321301","https://openalex.org/W2070073612","https://openalex.org/W2070239760","https://openalex.org/W2070330648","https://openalex.org/W2070537581","https://openalex.org/W2070831180","https://openalex.org/W2071086763","https://openalex.org/W2071441005","https://openalex.org/W2071501400","https://openalex.org/W2072533027","https://openalex.org/W2072857739","https://openalex.org/W2073664268","https://openalex.org/W2075070130","https://openalex.org/W2075175017","https://openalex.org/W2075544898","https://openalex.org/W2076387647","https://openalex.org/W2077166550","https://openalex.org/W2079308685","https://openalex.org/W2079523764","https://openalex.org/W2081433490","https://openalex.org/W2081473078","https://openalex.org/W2081820384","https://openalex.org/W2082244953","https://openalex.org/W2083729524","https://openalex.org/W2084839776","https://openalex.org/W2085066785","https://openalex.org/W2085534722","https://openalex.org/W2085637348","https://openalex.org/W2091136828","https://openalex.org/W2091155265","https://openalex.org/W2091699835","https://openalex.org/W2092026879","https://openalex.org/W2092175057","https://openalex.org/W2092230974","https://openalex.org/W2092351890","https://openalex.org/W2093002075","https://openalex.org/W2093427592","https://openalex.org/W2095624868","https://openalex.org/W2096697992","https://openalex.org/W2096844540","https://openalex.org/W2098647073","https://openalex.org/W2100472054","https://openalex.org/W2101479257","https://openalex.org/W2102599235","https://openalex.org/W2102917730","https://openalex.org/W2103767591","https://openalex.org/W2104702732","https://openalex.org/W2106498825","https://openalex.org/W2108349365","https://openalex.org/W2111249775","https://openalex.org/W2111356152","https://openalex.org/W2114024667","https://openalex.org/W2114508542","https://openalex.org/W2114579439","https://openalex.org/W2115171894","https://openalex.org/W2116213179","https://openalex.org/W2117072114","https://openalex.org/W2117173394","https://openalex.org/W2117330188","https://openalex.org/W2117434487","https://openalex.org/W2119311998","https://openalex.org/W2120967544","https://openalex.org/W2121066085","https://openalex.org/W2121985216","https://openalex.org/W2124583402","https://openalex.org/W2124750119","https://openalex.org/W2125365332","https://openalex.org/W2126018286","https://openalex.org/W2127277431","https://openalex.org/W2128375100","https://openalex.org/W2128692282","https://openalex.org/W2129042241","https://openalex.org/W2129652422","https://openalex.org/W2132100443","https://openalex.org/W2132249622","https://openalex.org/W2133701300","https://openalex.org/W2133767887","https://openalex.org/W2135636849","https://openalex.org/W2136434181","https://openalex.org/W2136739054","https://openalex.org/W2137633625","https://openalex.org/W2139301092","https://openalex.org/W2140206032","https://openalex.org/W2142325518","https://openalex.org/W2142466527","https://openalex.org/W2143559788","https://openalex.org/W2144121899","https://openalex.org/W2144164884","https://openalex.org/W2144343392","https://openalex.org/W2144666203","https://openalex.org/W2144794655","https://openalex.org/W2148207593","https://openalex.org/W2148388239","https://openalex.org/W2148581165","https://openalex.org/W2149452073","https://openalex.org/W2149614036","https://openalex.org/W2152343731","https://openalex.org/W2153382500","https://openalex.org/W2153810573","https://openalex.org/W2154021943","https://openalex.org/W2154652266","https://openalex.org/W2155439940","https://openalex.org/W2156452836","https://openalex.org/W2158033500","https://openalex.org/W2158266568","https://openalex.org/W2158771952","https://openalex.org/W2159298101","https://openalex.org/W2159577916","https://openalex.org/W2161052699","https://openalex.org/W2161643159","https://openalex.org/W2162074340","https://openalex.org/W2162918149","https://openalex.org/W2163502722","https://openalex.org/W2164058746","https://openalex.org/W2164134751","https://openalex.org/W2164643779","https://openalex.org/W2165239123","https://openalex.org/W2167863101","https://openalex.org/W2167985627","https://openalex.org/W2168730873","https://openalex.org/W2168909179","https://openalex.org/W2169144108","https://openalex.org/W2169903394","https://openalex.org/W2170624303","https://openalex.org/W2170740776","https://openalex.org/W2171017866","https://openalex.org/W2171253958","https://openalex.org/W2174944208","https://openalex.org/W2175531321","https://openalex.org/W2314717281","https://openalex.org/W2337369895","https://openalex.org/W2435054337","https://openalex.org/W2443671149","https://openalex.org/W2999074976"],"related_works":["https://openalex.org/W2136538133","https://openalex.org/W6957924182","https://openalex.org/W2544864348","https://openalex.org/W2777582776","https://openalex.org/W2034741116","https://openalex.org/W2050397302","https://openalex.org/W7098124582","https://openalex.org/W2100472054","https://openalex.org/W1592465044","https://openalex.org/W2268508334"],"abstract_inverted_index":{"Transposable":[0],"DNA":[1,55,79,171,182],"elements":[2],"constitute":[3],"a":[4,82,89,101,113,118,176,327],"class":[5],"of":[6,42,49,66,105,123,138,155,160,190,250,289,323,331,357,366,378],"discrete":[7],"genome":[8],"segments,":[9],"which":[10,99,124],"use":[11],"fundamentally":[12],"similar":[13],"reactions":[14],"for":[15,25,201,213,257,270,315,354,363],"their":[16,74,367],"movement":[17],"within":[18,112],"and":[19,35,129,174,198,341,374],"between":[20],"genomes":[21,41],"without":[22],"the":[23,29,40,67,76,106,120,136,158,161,209,247,262,287,293,300,303,335,342,364,375,379],"need":[24],"sequence":[26,248],"homology":[27],"to":[28,51,179,227,292,312],"target":[30],"site.":[31],"Transposons":[32],"are":[33,143],"ubiquitous":[34],"abundant":[36],"residents":[37],"scattered":[38],"throughout":[39],"virtually":[43],"all":[44],"living":[45],"organisms.":[46],"The":[47,64,93,149,238,321],"ability":[48],"transposons":[50],"integrate":[52],"into":[53],"various":[54],"sequences":[56],"is":[57,71,100],"utilized":[58],"as":[59,117,371],"tools":[60,141],"in":[61,146,157,235,317,338,347],"molecular":[62],"biology.":[63],"similarity":[65],"transposition":[68,110,183,211,272,319],"reaction":[69],"mechanisms":[70],"reflected":[72],"by":[73,222],"enzymes,":[75],"transposases.":[77,108],"Many":[78],"transposases":[80,360],"share":[81],"conserved":[83],"RNase":[84,348],"H-like":[85,349],"fold":[86,350],"that":[87,142,276],"contains":[88,125],"catalytic":[90],"DDE":[91,107,359],"motif.":[92],"bacteriophage":[94],"Mu":[95,109,139,294,316],"encodes":[96],"MuA":[97,127,156,202,219,236,265,277,308,332],"transposase,":[98],"well":[102],"characterized":[103,187],"member":[104],"proceeds":[111],"nucleoprotein":[114],"complex":[115],"known":[116],"transpososome,":[119],"minimal":[121],"core":[122],"four":[126],"molecules":[128],"two":[130],"synapsed":[131],"transposon":[132,140],"ends.":[133],"It":[134],"forms":[135],"basis":[137],"widely":[144],"used":[145,194,199,353,362],"genetics/genomics":[147],"applications.":[148,320],"study":[150,166,325,340],"reveals":[151],"important":[152],"structure-function":[153,220,240,329],"relationships":[154,221],"context":[159],"transpososome.":[162],"In":[163,260],"addition,":[164,261],"this":[165,324,339],"critically":[167],"evaluates":[168],"different":[169,263],"random":[170,195,264],"mutagenesis":[172,196],"methods":[173,197,336],"describes":[175],"universal":[177],"assay":[178,212],"quantitatively":[180],"analyse":[181],"events.":[184],"Initially,":[185],"we":[186,207,297],"mutagenic":[188],"properties":[189],"several":[191,307],"most":[192],"commonly":[193],"them":[200],"mutant":[203,214,266,278],"library":[204,226],"generation.":[205],"Then,":[206],"established":[208],"quantitative":[210],"screening":[215],"purposes.":[216],"We":[217,253,274],"analysed":[218],"scanning":[223],"pentapeptide":[224],"insertion":[225,229,232],"dissect":[228],"tolerant":[230],"against":[231],"intolerant":[233],"regions":[234],"structure.":[237],"comprehensive":[239,328],"analysis":[241],"was":[242],"complemented":[243],"with":[244,280],"data":[245],"from":[246],"alignment":[249],"protein":[251,258],"homologues.":[252],"identified":[254],"potential":[255],"sites":[256],"modifications.":[259],"libraries":[267],"were":[268,310],"screened":[269],"increased":[271],"frequency.":[273],"show":[275],"variants":[279],"enhanced":[281],"activity":[282,304,343],"could":[283,298],"be":[284,313,352],"generated.":[285],"By":[286],"mapping":[288],"hyperactivity-inducing":[290],"substitutions":[291,345],"transpososome":[295],"structure,":[296],"characterize":[299],"factors":[301],"behind":[302],"enhancement.":[305],"Furthermore,":[306],"mutants":[309],"discovered":[311,346],"valuable":[314],"vitro":[318],"results":[322],"provide":[326],"map":[330],"transposase.":[333],"Moreover,":[334],"described":[337],"enhancing":[344],"can":[351],"mechanistic":[355],"analyses":[356,365],"other":[358],"or":[361],"close":[368],"relatives":[369],"such":[370],"HIV":[372],"integrase":[373],"RAG":[376],"recombinase":[377],"V(D)J":[380],"recombination.":[381]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}