{"id":"https://openalex.org/W2187311535","doi":null,"title":"More Efficient Tissue Lysis and Protein Digestion with Lower Concentration of Denaturant Using Pressure Cycling Technology","display_name":"More Efficient Tissue Lysis and Protein Digestion with Lower Concentration of Denaturant Using Pressure Cycling Technology","publication_year":2015,"publication_date":"2015-01-01","ids":{"openalex":"https://openalex.org/W2187311535","mag":"2187311535"},"language":"en","primary_location":{"id":"mag:2187311535","doi":null,"is_oa":false,"landing_page_url":"http://www.pressurebiosciences.com/downloads/publications/2015-03/Enzymes-and-urea-US-HUPO-POSTER_Final.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Wen Bing Yan","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Wen Bing Yan","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5042876589","display_name":"Vera Gross","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Vera Gross","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"Shiying Shao","orcid":"https://orcid.org/0000-0001-7077-5184","observed_orcids":["https://orcid.org/0000-0001-7077-5184"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Shiying Shao","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0001-7077-5184","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110607332","display_name":"Alexander V. Lazarev","orcid":"https://orcid.org/0009-0005-1490-9025","observed_orcids":["https://orcid.org/0009-0005-1490-9025"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Alexander Lazarev","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002051017","display_name":"Tiannan Guo","orcid":"https://orcid.org/0000-0003-3869-7651","observed_orcids":["https://orcid.org/0000-0003-3869-7651"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Tiannan Guo","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-3869-7651","affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5053361384","display_name":"Ruedi H. Aebersold","orcid":"https://orcid.org/0000-0002-9576-3267","observed_orcids":["https://orcid.org/0000-0002-9576-3267","https://orcid.org/0000-0003-0827-3495"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Ruedi Aebersold","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-9576-3267","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.05381835,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.9452999830245972,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.9452999830245972,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11455","display_name":"Microbial Inactivation Methods","score":0.006200000178068876,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.0038999998942017555,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/tissue-lysis","display_name":"tissue lysis","score":0.9620000123977661},{"id":"https://openalex.org/keywords/protein-digestion","display_name":"protein digestion","score":0.9779999852180481},{"id":"https://openalex.org/keywords/denaturant-concentration","display_name":"denaturant concentration","score":0.8259999752044678},{"id":"https://openalex.org/keywords/protein-extraction","display_name":"protein extraction","score":0.49799999594688416},{"id":"https://openalex.org/keywords/proteomics-sample-preparation","display_name":"proteomics sample preparation","score":0.6669999957084656}],"concepts":[{"id":"https://openalex.org/C191935318","wikidata":"https://www.wikidata.org/wiki/Q148","display_name":"China","level":2,"score":0.4476524591445923},{"id":"https://openalex.org/C60798267","wikidata":"https://www.wikidata.org/wiki/Q1226939","display_name":"Division (mathematics)","level":2,"score":0.436413049697876},{"id":"https://openalex.org/C161191863","wikidata":"https://www.wikidata.org/wiki/Q199655","display_name":"Library science","level":1,"score":0.3952864408493042},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.3443915843963623},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.14617598056793213},{"id":"https://openalex.org/C205649164","wikidata":"https://www.wikidata.org/wiki/Q1071","display_name":"Geography","level":0,"score":0.1344912350177765},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.12330618500709534},{"id":"https://openalex.org/C166957645","wikidata":"https://www.wikidata.org/wiki/Q23498","display_name":"Archaeology","level":1,"score":0.06810665130615234},{"id":"https://openalex.org/C94375191","wikidata":"https://www.wikidata.org/wiki/Q11205","display_name":"Arithmetic","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:2187311535","doi":null,"is_oa":false,"landing_page_url":"http://www.pressurebiosciences.com/downloads/publications/2015-03/Enzymes-and-urea-US-HUPO-POSTER_Final.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":4,"referenced_works":["https://openalex.org/W2033047611","https://openalex.org/W2050163368","https://openalex.org/W2085180681","https://openalex.org/W2088520276"],"related_works":["https://openalex.org/W2804832840","https://openalex.org/W4290207280","https://openalex.org/W2085180681","https://openalex.org/W2168848859","https://openalex.org/W176970103","https://openalex.org/W2405126578","https://openalex.org/W2088520276","https://openalex.org/W4311652001","https://openalex.org/W1012904387","https://openalex.org/W2237222323"],"abstract_inverted_index":{"Wen":[0],"Yan1,2,":[1],"Vera":[2],"Gross3,":[3],"Shiying":[4],"Shao1,4,":[5],"Alexander":[6],"Lazarev3,":[7],"Tiannan":[8],"Guo":[9],"1,":[10],"Ruedi":[11],"Aebersold1,5":[12],"1":[13],"Department":[14],"of":[15,18,27,33,51,57,67,70],"Biology,":[16,21],"Institute":[17],"Molecular":[19],"Systems":[20],"ETH":[22],"Zurich,":[23,71,72],"Switzerland.":[24],"2":[25],"Division":[26,50],"Geriatrics,":[28],"Tongji":[29,53],"Hospital,":[30,54],"Huazhong":[31,55],"University":[32,56,69],"Science":[34,58],"&":[35,59],"Technology,":[36,60],"Wuhan,":[37,61],"430030,":[38,62],"PR":[39,63],"China.":[40,64],"3":[41],"Pressure":[42],"BioSciences,":[43],"Inc.":[44],"South":[45],"Easton,":[46],"Ma.":[47],"USA":[48],"4":[49],"Endocrinology,":[52],"5":[65],"Faculty":[66],"Science,":[68],"Switzerland":[73]},"counts_by_year":[],"updated_date":"2026-11-05T12:25:35.769452","created_date":"2016-06-24T00:00:00"}