{"id":"https://openalex.org/W2187293186","doi":"https://doi.org/10.5858/2000-124-802-aihcat","title":"Apoptosis in HL-60 Cells and Topoisomerase I In Vivo","display_name":"Apoptosis in HL-60 Cells and Topoisomerase I In Vivo","publication_year":2000,"publication_date":"2000-06-01","ids":{"openalex":"https://openalex.org/W2187293186","doi":"https://doi.org/10.5858/2000-124-802-aihcat","mag":"2187293186","pmid":"https://pubmed.ncbi.nlm.nih.gov/10835507"},"language":"en","primary_location":{"id":"doi:10.5858/2000-124-802-aihcat","doi":"https://doi.org/10.5858/2000-124-802-aihcat","is_oa":false,"landing_page_url":"https://doi.org/10.5858/2000-124-802-aihcat","pdf_url":null,"source":{"id":"https://openalex.org/S139062638","display_name":"Archives of Pathology & Laboratory Medicine","issn_l":"0003-9985","issn":["0003-9985","1543-2165"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320259","host_organization_name":"American Medical Association","host_organization_lineage":["https://openalex.org/P4310320259"],"host_organization_lineage_names":["American Medical Association"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Archives of Pathology & Laboratory Medicine","raw_type":"journal-article"},"volume":null,"type":"letter","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5014597522","display_name":"Keith R. Jerome","orcid":"https://orcid.org/0000-0002-8212-3789","observed_orcids":["https://orcid.org/0000-0002-8212-3789"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Keith R. Jerome","raw_affiliation_strings":["Virology DivisionDepartment of Laboratory MedicineUniversity of WashingtonSeattle, WA 98109"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Virology DivisionDepartment of Laboratory MedicineUniversity of WashingtonSeattle, WA 98109","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5100370673","display_name":"Zheng Chen","orcid":"https://orcid.org/0000-0002-6582-847X","observed_orcids":["https://orcid.org/0000-0002-6582-847X"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Zheng Chen","raw_affiliation_strings":["Virology DivisionDepartment of Laboratory MedicineUniversity of WashingtonSeattle, WA 98109"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Virology DivisionDepartment of Laboratory MedicineUniversity of WashingtonSeattle, WA 98109","institution_ids":["https://openalex.org/I201448701"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I201448701"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":7,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"124","issue":"6","first_page":"802","last_page":"803"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10294","display_name":"Cell death mechanisms and regulation","score":0.9574999809265137,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10294","display_name":"Cell death mechanisms and regulation","score":0.9574999809265137,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11845","display_name":"Cancer therapeutics and mechanisms","score":0.03799999877810478,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12356","display_name":"Bioactive Compounds and Antitumor Agents","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/3005","display_name":"Toxicology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/apoptosis","display_name":"apoptosis","score":0.9990000128746033},{"id":"https://openalex.org/keywords/hl-60-cells","display_name":"HL-60 cells","score":0.996999979019165},{"id":"https://openalex.org/keywords/topoisomerase-i","display_name":"topoisomerase I","score":0.9929999709129333},{"id":"https://openalex.org/keywords/topoisomerase-i-inhibition","display_name":"topoisomerase I inhibition","score":0.7160000205039978},{"id":"https://openalex.org/keywords/in-vivo-drug-effects","display_name":"in vivo drug effects","score":0.4830000102519989}],"concepts":[{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.8002901077270508},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.7079671621322632},{"id":"https://openalex.org/C147897179","wikidata":"https://www.wikidata.org/wiki/Q407828","display_name":"Topoisomerase","level":3,"score":0.5713424682617188},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.47318291664123535},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4166216552257538},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.40810444951057434},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.36888980865478516},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.36109960079193115},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.32354289293289185},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.19093051552772522},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.17186444997787476}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001562","descriptor_name":"Benzimidazoles","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005347","descriptor_name":"Fibroblasts","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D005456","descriptor_name":"Fluorescent Dyes","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014407","descriptor_name":"Tumor Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017209","descriptor_name":"Apoptosis","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D018922","descriptor_name":"HL-60 Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D059004","descriptor_name":"Topoisomerase I Inhibitors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.5858/2000-124-802-aihcat","doi":"https://doi.org/10.5858/2000-124-802-aihcat","is_oa":false,"landing_page_url":"https://doi.org/10.5858/2000-124-802-aihcat","pdf_url":null,"source":{"id":"https://openalex.org/S139062638","display_name":"Archives of Pathology & Laboratory Medicine","issn_l":"0003-9985","issn":["0003-9985","1543-2165"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320259","host_organization_name":"American Medical Association","host_organization_lineage":["https://openalex.org/P4310320259"],"host_organization_lineage_names":["American Medical Association"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Archives of Pathology & Laboratory Medicine","raw_type":"journal-article"},{"id":"pmid:10835507","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10835507","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Archives of pathology & laboratory medicine","raw_type":"Letter"},{"id":"mag:2187293186","doi":null,"is_oa":false,"landing_page_url":"http://europepmc.org/abstract/MED/10835507","pdf_url":null,"source":{"id":"https://openalex.org/S139062638","display_name":"Archives of Pathology & Laboratory Medicine","issn_l":"0003-9985","issn":["0003-9985","1543-2165"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320259","host_organization_name":"American Medical Association","host_organization_lineage":["https://openalex.org/P4310320259"],"host_organization_lineage_names":["American Medical Association"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Archives of Pathology & Laboratory Medicine","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.5683000087738037}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":5,"referenced_works":["https://openalex.org/W265942891","https://openalex.org/W1898156904","https://openalex.org/W2118793944","https://openalex.org/W2383183184","https://openalex.org/W6709936283"],"related_works":["https://openalex.org/W1898156904","https://openalex.org/W2383183184","https://openalex.org/W3029072627","https://openalex.org/W7097948517","https://openalex.org/W4250214697","https://openalex.org/W1944457483","https://openalex.org/W1985370516","https://openalex.org/W1607033379","https://openalex.org/W2509845469","https://openalex.org/W2015181708"],"abstract_inverted_index":{"To":[0],"the":[1,8,38,47,79,152,180,230,233,239,243,269,272,280,297,300,368,392,429],"Editor.\u2014Zhang":[2],"et":[3,332,381],"al1":[4],"recently":[5],"reported":[6,329],"that":[7,50,78,90,182,328,390,428],"bisbenzimide":[9],"DNA":[10,160],"stain":[11,235,424],"Hoechst":[12,31,53,71,91,171,189,213,236,324,351,399,433],"33342":[13,32,54,72,172,214,237,400,434],"induces":[14],"apoptosis":[15,57,69,93,114,144,191,322,374,406,443,461],"in":[16,33,37,58,85,98,249,260,310,350,365,396,453],"HL-60":[17],"leukemic":[18],"cells.":[19,105],"This":[20],"follows":[21],"similar":[22,326],"reports":[23],"from":[24],"this":[25,340,423],"group":[26],"showing":[27],"proapoptotic":[28,430],"activity":[29],"of":[30,68,82,113,130,155,166,199,202,246,274,321,339,347,367,371,379,405,413,417,432],"hepatoma":[34],"cells2":[35],"and":[36,124,178,241,313,407],"differentiation-arrested":[39],"mouse":[40],"myocyte":[41],"line":[42],"BC3H-1.3":[43],"We":[44],"agree":[45],"with":[46,215],"authors'":[48,80],"conclusion":[49],"experiments":[51],"using":[52,170,212,398,422],"to":[55,65,143,188,194,303,327,362,373,384,438,460],"evaluate":[56],"these":[59,86,308],"cells":[60,107,184,353,439],"must":[61],"be":[62,174,186,266,385,426],"well":[63],"controlled":[64],"avoid":[66],"induction":[67,320,375],"by":[70,255,323,330,345,376],"itself.":[73],"However,":[74,420],"we":[75,204,315],"are":[76,108,118,139,383],"concerned":[77],"choice":[81],"cell":[83,240,270,281,455],"lines":[84,138],"studies":[87,112,198,211,389,397],"might":[88,185],"suggest":[89],"33342\u2013induced":[92,190],"is":[94,287,292,435,445],"only":[95],"a":[96,175,304,318,377,403,410,449],"concern":[97],"transformed":[99],"or":[100,103,223],"otherwise":[101],"immature":[102],"growth-arrested":[104],"Such":[106],"often":[109],"chosen":[110],"for":[111,387,394,408],"simply":[115],"because":[116],"they":[117,147],"easily":[119,440],"induced":[120,441],"into":[121,442],"programmed":[122,275],"death":[123,341],"typically":[125],"show":[126,150],"highly":[127],"stereotypical":[128],"features":[129,154],"apoptosis.":[131],"In":[132,229],"contrast,":[133],"many":[134],"nontransformed":[135],"primary":[136,220,224,311],"human":[137,221,225],"much":[140],"more":[141],"resistant":[142,187,459],"induction.":[145],"Furthermore,":[146],"may":[148],"not":[149,436],"all":[151],"classic":[153],"apoptosis,":[156],"such":[157,163,183,463],"as":[158,268,279,402,464],"oligonucleosomal":[159],"fragmentation.":[161],"For":[162],"cells,":[164],"evaluation":[165],"nuclear":[167,251,261,301],"morphologic":[168,262],"structure":[169,263],"can":[173,264],"useful":[176],"technique,":[177],"thus":[179,265],"assumption":[181],"would":[192],"lead":[193],"erroneous":[195],"conclusions.In":[196],"our":[197],"viral":[200],"inhibition":[201,416],"apoptosis,4":[203],"have":[205,316],"used":[206],"various":[207],"double-staining":[208],"techniques,":[209],"including":[210],"ethidium":[216,285,294],"bromide":[217,286,295],"on":[218],"either":[219],"fibroblasts":[222,312,372],"keratinocytes":[226],"(unpublished":[227],"data).":[228],"living":[231],"cell,":[232,298],"vital":[234],"enters":[238,296],"binds":[242],"minor":[244],"groove":[245],"DNA,5":[247],"resulting":[248],"blue":[250],"fluorescence":[252,256,302],"when":[253],"viewed":[254],"microscopy.":[257],"Subtle":[258],"changes":[259],"evaluated":[267],"begins":[271],"process":[273],"death.":[276],"As":[277],"long":[278],"maintains":[282],"membrane":[283,290],"integrity,":[284],"excluded.":[288],"Once":[289],"integrity":[291],"lost,":[293],"changing":[299],"light":[305,366],"violet.":[306],"Using":[307],"stains":[309],"keratinocytes,":[314],"noted":[317],"time-dependent":[319],"33342,":[325],"Zhang":[331],"al":[333,382],"(Figure,":[334,354],"A).":[335],"The":[336],"apoptotic":[337],"nature":[338],"has":[342],"been":[343],"confirmed":[344],"measurement":[346],"caspase":[348],"activation":[349],"33342\u2013treated":[352],"B).":[355],"These":[356],"findings":[357],"were":[358],"initially":[359],"somewhat":[360],"surprising":[361],"us,":[363],"especially":[364],"general":[369,450],"resistance":[370],"variety":[378],"stimuli.Zhang":[380],"commended":[386],"well-performed":[388],"illustrate":[391],"potential":[393,411],"artifact":[395],"staining":[401],"measure":[404],"defining":[409],"mechanism":[412],"action":[414],"via":[415],"topoisomerase":[418],"I.1\u20133":[419],"investigators":[421],"should":[425],"aware":[427],"effect":[431],"limited":[437],"but":[444],"rather":[446],"most":[447,454],"likely":[448],"phenomenon":[451],"seen":[452],"types,":[456],"even":[457],"those":[458],"induction,":[462],"fibroblasts.":[465]},"counts_by_year":[{"year":2026,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}