{"id":"https://openalex.org/W2186540893","doi":null,"title":"Identification of apolipoprotein E polymorphisms using temperature cycled primer oligo base extension and mass spectrometry.","display_name":"Identification of apolipoprotein E polymorphisms using temperature cycled primer oligo base extension and mass spectrometry.","publication_year":1997,"publication_date":"1997-07-01","ids":{"openalex":"https://openalex.org/W2186540893","mag":"2186540893","pmid":"https://pubmed.ncbi.nlm.nih.gov/9263732"},"language":"en","primary_location":{"id":"pmid:9263732","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9263732","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111531169","display_name":"Daniel P. Little","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"D P Little","raw_affiliation_strings":["Sequenom Instruments GmbH, Hamburg, Germany. danlittle@juno.com","Sequenom Instruments GmbH, Hamburg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Sequenom Instruments GmbH, Hamburg, Germany. danlittle@juno.com","institution_ids":[]},{"raw_affiliation_string":"Sequenom Instruments GmbH, Hamburg, Germany","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5043794130","display_name":"Andreas Dominik Braun","orcid":"https://orcid.org/0000-0002-6954-0633","observed_orcids":["https://orcid.org/0000-0002-6954-0633"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"A Braun","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5004829357","display_name":"Brigitte Darnhofer-Demar","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"B Darnhofer-Demar","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5110467207","display_name":"H. K\u00f6ster","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"H K\u00f6ster","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":2.0776,"has_fulltext":false,"cited_by_count":53,"citation_normalized_percentile":{"value":0.84377543,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"35","issue":"7","first_page":"545","last_page":"8"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.9218999743461609,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.9218999743461609,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12167","display_name":"Bacterial Identification and Susceptibility Testing","score":0.014399999752640724,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.006899999920278788,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/apoe-polymorphism","display_name":"APOE polymorphism","score":0.9139999747276306},{"id":"https://openalex.org/keywords/isothermal-dna-amplification","display_name":"isothermal DNA amplification","score":0.5640000104904175},{"id":"https://openalex.org/keywords/genotype-identification","display_name":"genotype identification","score":0.4000000059604645},{"id":"https://openalex.org/keywords/matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry","display_name":"matrix-assisted laser desorption/ionization time-of-flight mass spectrometry","score":0.8999999761581421},{"id":"https://openalex.org/keywords/automated-genotyping","display_name":"automated genotyping","score":0.5529999732971191}],"concepts":[{"id":"https://openalex.org/C194857396","wikidata":"https://www.wikidata.org/wiki/Q382878","display_name":"Primer extension","level":4,"score":0.7553586363792419},{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.7349890470504761},{"id":"https://openalex.org/C2777563447","wikidata":"https://www.wikidata.org/wiki/Q7243397","display_name":"Primer (cosmetics)","level":2,"score":0.6108564138412476},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5819380283355713},{"id":"https://openalex.org/C113196181","wikidata":"https://www.wikidata.org/wiki/Q485223","display_name":"Analytical Chemistry (journal)","level":2,"score":0.5686904788017273},{"id":"https://openalex.org/C40325409","wikidata":"https://www.wikidata.org/wiki/Q2360668","display_name":"Mass spectrum","level":3,"score":0.4192522466182709},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.37656158208847046},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.118649423122406},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.11607572436332703},{"id":"https://openalex.org/C3017666073","wikidata":"https://www.wikidata.org/wiki/Q1764062","display_name":"Base sequence","level":3,"score":0.07725310325622559},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001057","descriptor_name":"Apolipoproteins E","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":false},{"descriptor_ui":"D001057","descriptor_name":"Apolipoproteins E","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003062","descriptor_name":"Codon","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005069","descriptor_name":"Evaluation Studies as Topic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005838","descriptor_name":"Genotype","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011110","descriptor_name":"Polymorphism, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D019032","descriptor_name":"Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":2,"locations":[{"id":"pmid:9263732","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9263732","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies","raw_type":"Journal Article"},{"id":"mag:2186540893","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9263732","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.6240000128746033,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":31,"referenced_works":["https://openalex.org/W256414136","https://openalex.org/W1546262989","https://openalex.org/W1590095886","https://openalex.org/W1896684000","https://openalex.org/W1960011013","https://openalex.org/W1961904860","https://openalex.org/W1970164375","https://openalex.org/W1971106404","https://openalex.org/W1974830441","https://openalex.org/W1976026508","https://openalex.org/W1982660567","https://openalex.org/W1995722498","https://openalex.org/W2007256938","https://openalex.org/W2027709132","https://openalex.org/W2028925976","https://openalex.org/W2036362108","https://openalex.org/W2036940947","https://openalex.org/W2038977574","https://openalex.org/W2039158755","https://openalex.org/W2051079162","https://openalex.org/W2058065325","https://openalex.org/W2066644955","https://openalex.org/W2069464326","https://openalex.org/W2081511962","https://openalex.org/W2089166604","https://openalex.org/W2089386052","https://openalex.org/W2111774300","https://openalex.org/W2111953437","https://openalex.org/W2132528357","https://openalex.org/W2154296702","https://openalex.org/W2154838738"],"related_works":["https://openalex.org/W2038977574","https://openalex.org/W1998399139","https://openalex.org/W1970160615","https://openalex.org/W2158703636","https://openalex.org/W2037561692","https://openalex.org/W2232396530","https://openalex.org/W1836037828","https://openalex.org/W2111953437","https://openalex.org/W2243633824","https://openalex.org/W2027360989"],"abstract_inverted_index":{"The":[0,115,132],"isothermal":[1],"Primer":[2],"Oligo":[3],"Base":[4],"Extension":[5],"(PROBE)":[6],"reaction":[7],"combined":[8],"with":[9],"matrix-assisted":[10],"laser":[11],"desorption/ionization":[12],"time-of-flight":[13],"mass":[14,102,113],"spectrometry":[15],"for":[16,60,82,126,157],"diagnostic":[17,42,103,136],"product":[18,43,137],"detection":[19,111],"as":[20],"recently":[21],"introduced":[22],"by":[23,87,112,141],"our":[24],"group":[25],"is":[26],"modified":[27],"to":[28,93,110,151],"incorporate":[29],"temperature":[30,46,116],"cycling":[31,143],"during":[32],"the":[33,49,94,142,152],"primer":[34,140],"extension":[35],"step,":[36],"resulting":[37],"in":[38],"enhanced":[39],"levels":[40],"of":[41,51,62,90,96,135,161],"generation.":[44],"Utilizing":[45],"cycled":[47,117],"PROBE,":[48],"identities":[50],"two":[52],"apolipoprotein":[53],"E":[54],"polymorphisms":[55],"(codons":[56],"112":[57],"and":[58,72,123],"158)":[59],"differentiation":[61],"epsilon":[63,66,69,73],"2/epsilon":[64],"3,":[65,68],"3/epsilon":[67,70],"4,":[71],"4/epsilon":[74],"4":[75],"genotypes":[76],"were":[77],"simultaneously":[78],"determined.":[79],"Primers":[80],"specific":[81],"each":[83],"site":[84],"are":[85],"extended":[86],"a":[88],"series":[89],"bases":[91],"unique":[92],"identity":[95],"that":[97],"variable":[98],"site,":[99],"producing":[100],"low":[101],"products":[104],"(M(r)":[105],"<":[106],"9000)":[107],"highly":[108],"amenable":[109],"spectrometry.":[114],"PROBE":[118],"method":[119,144],"has":[120],"yielded":[121],"unambiguous":[122],"correct":[124],"diagnoses":[125],"all":[127],"samples":[128],"tested":[129],"thus":[130],"far.":[131],"increased":[133,153],"amount":[134],"generated":[138],"per":[139],"makes":[145],"possible":[146],"faster":[147],"spectrum":[148],"acquisition":[149],"due":[150],"signal":[154],"intensity,":[155],"critical":[156],"future":[158],"automated":[159],"measurement":[160],"such":[162],"samples.":[163]},"counts_by_year":[{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}