{"id":"https://openalex.org/W2185035596","doi":null,"title":"Production, Purification and Characterization of Protease by Aspergillus flavus under Solid State Fermentation","display_name":"Production, Purification and Characterization of Protease by Aspergillus flavus under Solid State Fermentation","publication_year":2011,"publication_date":"2011-01-01","ids":{"openalex":"https://openalex.org/W2185035596","mag":"2185035596"},"language":"en","primary_location":{"id":"mag:2185035596","doi":null,"is_oa":false,"landing_page_url":"http://jjbs.hu.edu.jo/files/v4n3/Paper_number4_modified-Final.pdf","pdf_url":null,"source":{"id":"https://openalex.org/S2764627590","display_name":"Jordan Journal of Biological Sciences","issn_l":"1995-6673","issn":["1995-6673","2307-7166"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":["poland-20","russia-white-list-2"],"host_organization":"https://openalex.org/P4310319224","host_organization_name":"Hashemite University","host_organization_lineage":["https://openalex.org/P4310319224"],"host_organization_lineage_names":["Hashemite University"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Jordan Journal of Biological Sciences","raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5112001956","display_name":"Chinnasamy Muthulakshmi","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Chinnasamy Muthulakshmi","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109540643","display_name":"D. Gomathi","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"D. Gomathi","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101929130","display_name":"Dugganaboyana Guru Kumar","orcid":"https://orcid.org/0000-0002-1847-6870","observed_orcids":["https://orcid.org/0000-0002-1847-6870"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Dugganaboyana Guru Kumar","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-1847-6870","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012486025","display_name":"Ganesan Ravikumar","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Ganesan Ravikumar","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057689668","display_name":"Manokaran Kalaiselvi","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M. Kalaiselvi","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":null,"display_name":"Chandrasekar Uma","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Chandrasekar Uma","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":3.4937,"has_fulltext":false,"cited_by_count":82,"citation_normalized_percentile":{"value":0.9153213,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"4","issue":"3","first_page":"137","last_page":"148"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10171","display_name":"Biofuel production and bioconversion","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protease-production","display_name":"protease production","score":0.9679999947547913},{"id":"https://openalex.org/keywords/aspergillus-flavus","display_name":"Aspergillus flavus","score":1.0},{"id":"https://openalex.org/keywords/solid-state-fermentation","display_name":"solid-state fermentation","score":1.0},{"id":"https://openalex.org/keywords/wheat-bran","display_name":"wheat bran","score":0.7649999856948853},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.9319999814033508},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.8410000205039978},{"id":"https://openalex.org/keywords/thermal-stability","display_name":"thermal stability","score":0.6790000200271606},{"id":"https://openalex.org/keywords/metal-ion-effects-on-enzyme-activity","display_name":"metal ion effects on enzyme activity","score":0.9240000247955322},{"id":"https://openalex.org/keywords/nitrogen-source-optimization","display_name":"nitrogen source optimization","score":0.5019999742507935}],"concepts":[{"id":"https://openalex.org/C2779942233","wikidata":"https://www.wikidata.org/wiki/Q7557796","display_name":"Solid-state fermentation","level":3,"score":0.9449034929275513},{"id":"https://openalex.org/C2776873940","wikidata":"https://www.wikidata.org/wiki/Q188672","display_name":"Bran","level":3,"score":0.8889604806900024},{"id":"https://openalex.org/C2777292910","wikidata":"https://www.wikidata.org/wiki/Q137096","display_name":"Aspergillus flavus","level":2,"score":0.8123677968978882},{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.772520899772644},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6595214605331421},{"id":"https://openalex.org/C100544194","wikidata":"https://www.wikidata.org/wiki/Q41760","display_name":"Fermentation","level":2,"score":0.647072434425354},{"id":"https://openalex.org/C2777289219","wikidata":"https://www.wikidata.org/wiki/Q7632154","display_name":"Substrate (aquarium)","level":2,"score":0.6045345664024353},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.5833871364593506},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.5365536212921143},{"id":"https://openalex.org/C537208039","wikidata":"https://www.wikidata.org/wiki/Q627","display_name":"Nitrogen","level":2,"score":0.47048619389533997},{"id":"https://openalex.org/C199164860","wikidata":"https://www.wikidata.org/wiki/Q6822745","display_name":"Metal ions in aqueous solution","level":3,"score":0.4669642746448517},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.4574652314186096},{"id":"https://openalex.org/C28212737","wikidata":"https://www.wikidata.org/wiki/Q5381543","display_name":"Enzyme assay","level":3,"score":0.44169536232948303},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3220142126083374},{"id":"https://openalex.org/C544153396","wikidata":"https://www.wikidata.org/wiki/Q11426","display_name":"Metal","level":2,"score":0.2075134813785553},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.17069894075393677},{"id":"https://openalex.org/C206139338","wikidata":"https://www.wikidata.org/wiki/Q192355","display_name":"Raw material","level":2,"score":0.12069904804229736},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.09253653883934021},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:2185035596","doi":null,"is_oa":false,"landing_page_url":"http://jjbs.hu.edu.jo/files/v4n3/Paper_number4_modified-Final.pdf","pdf_url":null,"source":{"id":"https://openalex.org/S2764627590","display_name":"Jordan Journal of Biological Sciences","issn_l":"1995-6673","issn":["1995-6673","2307-7166"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":["poland-20","russia-white-list-2"],"host_organization":"https://openalex.org/P4310319224","host_organization_name":"Hashemite University","host_organization_lineage":["https://openalex.org/P4310319224"],"host_organization_lineage_names":["Hashemite University"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Jordan Journal of Biological Sciences","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.6000000238418579,"display_name":"Responsible consumption and production","id":"https://metadata.un.org/sdg/12"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":30,"referenced_works":["https://openalex.org/W1033786336","https://openalex.org/W1577604841","https://openalex.org/W1578691068","https://openalex.org/W1775749144","https://openalex.org/W1965624337","https://openalex.org/W1975273371","https://openalex.org/W1979479889","https://openalex.org/W1980029150","https://openalex.org/W1990726117","https://openalex.org/W1994764596","https://openalex.org/W2019849187","https://openalex.org/W2051538984","https://openalex.org/W2056904702","https://openalex.org/W2059361238","https://openalex.org/W2068788016","https://openalex.org/W2076153011","https://openalex.org/W2078411515","https://openalex.org/W2102628698","https://openalex.org/W2119581418","https://openalex.org/W2128115994","https://openalex.org/W2129845291","https://openalex.org/W2134114750","https://openalex.org/W2185613487","https://openalex.org/W2232439086","https://openalex.org/W2465412344","https://openalex.org/W2470949196","https://openalex.org/W2510009561","https://openalex.org/W2523667078","https://openalex.org/W2542797621","https://openalex.org/W3148461650"],"related_works":["https://openalex.org/W3048682578","https://openalex.org/W3113084602","https://openalex.org/W7014733370","https://openalex.org/W2557827152","https://openalex.org/W2472142989","https://openalex.org/W7171284249","https://openalex.org/W3204066385","https://openalex.org/W2383124969","https://openalex.org/W3098967467","https://openalex.org/W2183228962"],"abstract_inverted_index":{"Protease":[0],"production":[1,32,52],"under":[2],"solid":[3],"state":[4],"fermentation":[5],"(SSF)":[6],"was":[7,98,126,137],"investigated":[8],"using":[9,36],"isolated":[10],"Aspergillus":[11,34],"flavus.":[12],"Different":[13],"agroindustrial":[14],"waste":[15],"products":[16],"were":[17,53],"evaluated":[18],"to":[19,55,100,128],"check":[20],"the":[21,46,95],"possibility":[22],"of":[23,26,60,89,110,116,121],"potential":[24],"utilization":[25],"substrates":[27],"in":[28],"SSF":[29],"for":[30,49],"protease":[31,51],"by":[33,91,103,139,150],"flavus":[35],"wheat":[37],"bran":[38],"as":[39,78],"a":[40,107],"substrate.":[41],"The":[42,81,123],"results":[43],"showed":[44],"that":[45],"optimum":[47],"conditions":[48],"maximum":[50],"found":[54,127],"be":[56,101,129],"7":[57],"th":[58],"day":[59],"incubation":[61],"at":[62,119,131],"pH":[63,120],"5.0,":[64],"temperature":[65],"30":[66],"o":[67],"C;":[68],"inoculum":[69],"size":[70],"3%;":[71],"substrate":[72],"concentration":[73],"3%":[74,76],"and":[75,94,113,135,145,148,153],"KNO3":[77],"nitrogen":[79],"source.":[80],"purified":[82],"enzyme":[83,124],"produced":[84],"5.8":[85],"fold":[86],"with":[87],"recovery":[88],"3.2%":[90],"DEAE-column":[92],"chromatography":[93],"molecular":[96],"weight":[97],"estimated":[99],"46kDa":[102],"SDS-PAGE.":[104],"It":[105],"has":[106],"Vmax":[108],"value":[109,115],"60.0":[111],"U/mg":[112],"Km":[114],"0.6":[117],"mg/ml":[118],"7.":[122],"activity":[125],"stable":[130],"50":[132],"0":[133],"C":[134],"it":[136],"stimulated":[138],"metal":[140],"ions":[141],"like":[142],"Cu":[143],"2+":[144,147,152,155],"Zn":[146],"inhibited":[149],"Ca":[151],"Mg":[154],".":[156]},"counts_by_year":[{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":7},{"year":2022,"cited_by_count":8},{"year":2021,"cited_by_count":10},{"year":2020,"cited_by_count":6},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":8},{"year":2017,"cited_by_count":7},{"year":2016,"cited_by_count":5},{"year":2015,"cited_by_count":7},{"year":2014,"cited_by_count":6},{"year":2013,"cited_by_count":6},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}