{"id":"https://openalex.org/W2182929920","doi":null,"title":"Cytotoxic effects of antiglypican-3 against HepG2 cell line","display_name":"Cytotoxic effects of antiglypican-3 against HepG2 cell line","publication_year":2013,"publication_date":"2013-01-01","ids":{"openalex":"https://openalex.org/W2182929920","mag":"2182929920"},"language":"en","primary_location":{"id":"mag:2182929920","doi":null,"is_oa":false,"landing_page_url":"https://www.japsonline.com/admin/php/uploads/1130_pdf.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5053351285","display_name":"Randa Ahmed Zaghloul","orcid":"https://orcid.org/0000-0001-7827-195X","observed_orcids":["https://orcid.org/0000-0001-7827-195X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Randa A. Zaghloul","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028080816","display_name":"Mohammed M.H. Al-Gayyar","orcid":"https://orcid.org/0000-0003-4777-3919","observed_orcids":["https://orcid.org/0000-0003-4777-3919"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Mohammed M.H. Al-Gayyar","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-4777-3919","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5048793499","display_name":"Mamdouh M. El\u2010Shishtawy","orcid":"https://orcid.org/0000-0002-7888-354X","observed_orcids":["https://orcid.org/0000-0002-7888-354X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Mamdouh M. El\u2010Shishtawy","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5101974794","display_name":"Mohamed Ebrahim","orcid":"https://orcid.org/0000-0003-4474-4098","observed_orcids":["https://orcid.org/0000-0003-4474-4098"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Mohamed Ebrahim","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-4474-4098","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.1824,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":{"value":0.61309366,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10073","display_name":"Hepatocellular Carcinoma Treatment and Prognosis","score":0.6470000147819519,"subfield":{"id":"https://openalex.org/subfields/2721","display_name":"Hepatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10073","display_name":"Hepatocellular Carcinoma Treatment and Prognosis","score":0.6470000147819519,"subfield":{"id":"https://openalex.org/subfields/2721","display_name":"Hepatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11131","display_name":"Proteoglycans and glycosaminoglycans research","score":0.2782999873161316,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10966","display_name":"Liver physiology and pathology","score":0.005200000014156103,"subfield":{"id":"https://openalex.org/subfields/2721","display_name":"Hepatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/glypican-3","display_name":"glypican-3","score":0.9679999947547913},{"id":"https://openalex.org/keywords/hepatocellular-carcinoma","display_name":"hepatocellular carcinoma","score":0.9879999756813049},{"id":"https://openalex.org/keywords/hepg2-cells","display_name":"HepG2 cells","score":0.9929999709129333},{"id":"https://openalex.org/keywords/cytotoxicity","display_name":"cytotoxicity","score":0.8190000057220459},{"id":"https://openalex.org/keywords/apoptosis","display_name":"apoptosis","score":0.9309999942779541},{"id":"https://openalex.org/keywords/caspase-3","display_name":"caspase-3","score":0.996999979019165},{"id":"https://openalex.org/keywords/heparan-sulfate-proteoglycans","display_name":"heparan sulfate proteoglycans","score":0.9789999723434448},{"id":"https://openalex.org/keywords/sulfatase-2","display_name":"SULF2","score":0.9459999799728394},{"id":"https://openalex.org/keywords/cancer-therapy-targets","display_name":"cancer therapy targets","score":0.4390000104904175}],"concepts":[{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.7423595190048218},{"id":"https://openalex.org/C154317977","wikidata":"https://www.wikidata.org/wiki/Q376266","display_name":"Cytotoxic T cell","level":3,"score":0.7191178798675537},{"id":"https://openalex.org/C109316439","wikidata":"https://www.wikidata.org/wiki/Q246181","display_name":"Cytotoxicity","level":3,"score":0.6901001334190369},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.5754622220993042},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.5635137557983398},{"id":"https://openalex.org/C2777318727","wikidata":"https://www.wikidata.org/wiki/Q409464","display_name":"Lactate dehydrogenase","level":3,"score":0.5316677093505859},{"id":"https://openalex.org/C62112901","wikidata":"https://www.wikidata.org/wiki/Q189159","display_name":"Cell growth","level":2,"score":0.49714019894599915},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.470654159784317},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.4693777561187744},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4183042347431183},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.3599039912223816},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3271063566207886},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.3200639486312866},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.3170798420906067},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3022177219390869},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2649520933628082},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.12579485774040222},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.11574903130531311},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:2182929920","doi":null,"is_oa":false,"landing_page_url":"https://www.japsonline.com/admin/php/uploads/1130_pdf.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":1.0,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":28,"referenced_works":["https://openalex.org/W1717531886","https://openalex.org/W1964480542","https://openalex.org/W1964664406","https://openalex.org/W1971012938","https://openalex.org/W1972437169","https://openalex.org/W1996435247","https://openalex.org/W2013783679","https://openalex.org/W2032920239","https://openalex.org/W2033103149","https://openalex.org/W2034447209","https://openalex.org/W2042122379","https://openalex.org/W2049388096","https://openalex.org/W2059529313","https://openalex.org/W2061716929","https://openalex.org/W2068782912","https://openalex.org/W2081829534","https://openalex.org/W2083574179","https://openalex.org/W2086710913","https://openalex.org/W2088866343","https://openalex.org/W2094263167","https://openalex.org/W2106285087","https://openalex.org/W2126330431","https://openalex.org/W2132286877","https://openalex.org/W2134589440","https://openalex.org/W2151232299","https://openalex.org/W2158249242","https://openalex.org/W2170369650","https://openalex.org/W3139763557"],"related_works":["https://openalex.org/W2047179372","https://openalex.org/W2077593040","https://openalex.org/W4252198967","https://openalex.org/W2972800036","https://openalex.org/W3175709728","https://openalex.org/W2372884178","https://openalex.org/W4212963658","https://openalex.org/W2312339750","https://openalex.org/W1991713904","https://openalex.org/W2096368722"],"abstract_inverted_index":{"Article":[0],"history:":[1],"Received":[2],"on:":[3,6,9],"05/12/2013":[4],"Revised":[5],"16/12/2013":[7],"Accepted":[8],"23/12/2013":[10],"Available":[11],"online:":[12],"30/12/2013":[13],"For":[14],"decades,":[15],"various":[16],"systemic":[17],"therapies":[18],"have":[19,48],"been":[20,49],"explored":[21],"for":[22,73],"the":[23,30,36,159],"treatment":[24],"of":[25,40,80,194,198,207],"advanced":[26],"hepatocellular":[27],"carcinoma":[28],"(HCC),":[29],"fifth":[31],"most":[32],"common":[33],"cancer":[34],"and":[35,103,113,120,144,196],"third":[37],"leading":[38],"cause":[39],"cancer-related":[41],"deaths":[42],"worldwide.":[43],"Nevertheless,":[44],"no":[45],"satisfactory":[46],"results":[47],"obtained":[50],"so":[51],"far.":[52],"Glypican-3":[53],"(GPC-3)":[54],"is":[55],"a":[56,65,83,149,204],"cell-surface":[57],"heparan":[58],"sulfate":[59],"proteoglycans":[60],"(HSPGs)":[61],"that":[62],"emerged":[63],"as":[64,69,71],"promising":[66,205],"diagnostic":[67],"marker":[68],"well":[70],"target":[72,206],"therapy.":[74,209],"Therefore;":[75],"we":[76],"investigated":[77],"antitumor":[78],"activity":[79,164],"antiglypican-3":[81],"(antiGPC3),":[82],"specific":[84],"antibody":[85],"aginst":[86],"GPC-3,":[87,118],"against":[88],"HepG2,":[89],"human":[90],"HCC,":[91],"cell":[92,107,146],"line.":[93],"HepG2":[94,106,141,177],"cells":[95,142],"were":[96,124],"treated":[97],"with":[98],"AntiGPC3":[99],"at":[100],"(5,":[101],"10":[102],"20":[104],"\u03bcg/ml).":[105],"proliferation":[108],"was":[109,130,155],"measured":[110,125,161],"by":[111,126,132,162,192],"MTT":[112],"lactate":[114],"dehydrogenase":[115],"(LDH)":[116],"assays.":[117],"HSPG":[119,171],"sulfatase-2":[121],"(SULF2)":[122],"levels":[123],"ELISA.":[127],"Moreover,":[128],"apoptosis":[129,160],"assessed":[131],"measuring":[133],"Caspase-3":[134],"activity.":[135],"We":[136,179],"found":[137],"that,":[138,182],"antiGPC3":[139,154,169,183],"reduced":[140],"survival":[143],"showed":[145],"cytotoxicity":[147],"in":[148,165,176],"dose-dependent":[150],"manner.":[151],"In":[152],"addition,":[153],"able":[156],"to":[157],"increase":[158,197],"capase-3":[163],"hepG2":[166],"cells.":[167,178],"Finally,":[168],"restored":[170],"level":[172],"without":[173],"affecting":[174],"SULF2":[175],"can":[180,188],"conclude":[181],"possesses":[184],"cytotoxic":[185],"effects,":[186],"which":[187],"be":[189],"partially":[190],"explained":[191],"restoration":[193],"HSPGs":[195],"caspase-3":[199],"apoptotic":[200],"pathway.":[201],"GPC-3":[202],"represents":[203],"HCC":[208]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2014,"cited_by_count":1}],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2016-06-24T00:00:00"}