{"id":"https://openalex.org/W218269802","doi":null,"title":"Constraint-based modelling of mixed microbial populations: Application to polyhydroxyalkanoates production","display_name":"Constraint-based modelling of mixed microbial populations: Application to polyhydroxyalkanoates production","publication_year":2013,"publication_date":"2013-01-01","ids":{"openalex":"https://openalex.org/W218269802","mag":"218269802"},"language":"en","primary_location":{"id":"pmh:oai:run.unl.pt:10362/13111","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10362/13111","pdf_url":"https://run.unl.pt/bitstreams/3a06a73b-3659-408c-b7e3-0778790bd328/download","source":{"id":"https://openalex.org/S4306402433","display_name":"Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"doctoral thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://run.unl.pt/bitstreams/3a06a73b-3659-408c-b7e3-0778790bd328/download","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5015042586","display_name":"Filipa Pardelha","orcid":"https://orcid.org/0000-0001-6282-0272","observed_orcids":["https://orcid.org/0000-0001-6282-0272"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Filipa Pardelha","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0001-6282-0272","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5015042586"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10661","display_name":"biodegradable polymer synthesis and properties","score":0.5005000233650208,"subfield":{"id":"https://openalex.org/subfields/2502","display_name":"Biomaterials"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10661","display_name":"biodegradable polymer synthesis and properties","score":0.5005000233650208,"subfield":{"id":"https://openalex.org/subfields/2502","display_name":"Biomaterials"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.4787999987602234,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T14285","display_name":"Biopolymer Synthesis and Applications","score":0.00139999995008111,"subfield":{"id":"https://openalex.org/subfields/2402","display_name":"Applied Microbiology and Biotechnology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/polyhydroxyalkanoates","display_name":"polyhydroxyalkanoates","score":0.8809999823570251},{"id":"https://openalex.org/keywords/mixed-culture","display_name":"mixed culture","score":0.9539999961853027},{"id":"https://openalex.org/keywords/constraint-based-modeling","display_name":"constraint-based modeling","score":0.628000020980835},{"id":"https://openalex.org/keywords/flux-balance-analysis","display_name":"flux balance analysis","score":0.7549999952316284},{"id":"https://openalex.org/keywords/metabolic-flux-analysis","display_name":"metabolic flux analysis","score":0.9679999947547913},{"id":"https://openalex.org/keywords/volatile-fatty-acids","display_name":"volatile fatty acids","score":0.777999997138977},{"id":"https://openalex.org/keywords/sugarcane-molasses","display_name":"sugarcane molasses","score":0.9169999957084656},{"id":"https://openalex.org/keywords/dynamic-metabolic-model","display_name":"dynamic metabolic model","score":0.8169999718666077},{"id":"https://openalex.org/keywords/fermentation-optimization","display_name":"fermentation optimization","score":0.44999998807907104}],"concepts":[{"id":"https://openalex.org/C73447357","wikidata":"https://www.wikidata.org/wiki/Q425079","display_name":"Polyhydroxyalkanoates","level":3,"score":0.9726947546005249},{"id":"https://openalex.org/C2778348673","wikidata":"https://www.wikidata.org/wiki/Q739302","display_name":"Production (economics)","level":2,"score":0.5573722124099731},{"id":"https://openalex.org/C2776036281","wikidata":"https://www.wikidata.org/wiki/Q48769818","display_name":"Constraint (computer-aided design)","level":2,"score":0.49653440713882446},{"id":"https://openalex.org/C183696295","wikidata":"https://www.wikidata.org/wiki/Q2487696","display_name":"Biochemical engineering","level":1,"score":0.45559006929397583},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.23241662979125977},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.21540135145187378},{"id":"https://openalex.org/C162324750","wikidata":"https://www.wikidata.org/wiki/Q8134","display_name":"Economics","level":0,"score":0.15333521366119385},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.13469627499580383},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.08381408452987671},{"id":"https://openalex.org/C175444787","wikidata":"https://www.wikidata.org/wiki/Q39072","display_name":"Microeconomics","level":1,"score":0.07523322105407715},{"id":"https://openalex.org/C78519656","wikidata":"https://www.wikidata.org/wiki/Q101333","display_name":"Mechanical engineering","level":1,"score":0.06464499235153198}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:run.unl.pt:10362/13111","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10362/13111","pdf_url":"https://run.unl.pt/bitstreams/3a06a73b-3659-408c-b7e3-0778790bd328/download","source":{"id":"https://openalex.org/S4306402433","display_name":"Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"doctoral thesis"},{"id":"mag:218269802","doi":null,"is_oa":false,"landing_page_url":"https://run.unl.pt/bitstream/10362/13111/1/Pardelha_2013.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:run.unl.pt:10362/13111","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10362/13111","pdf_url":"https://run.unl.pt/bitstreams/3a06a73b-3659-408c-b7e3-0778790bd328/download","source":{"id":"https://openalex.org/S4306402433","display_name":"Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"doctoral thesis"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/12","display_name":"Responsible consumption and production","score":0.6000000238418579}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W218269802.pdf","grobid_xml":"https://content.openalex.org/works/W218269802.grobid-xml"},"referenced_works_count":104,"referenced_works":["https://openalex.org/W154451530","https://openalex.org/W1088503174","https://openalex.org/W1501676591","https://openalex.org/W1574470162","https://openalex.org/W1701869895","https://openalex.org/W1837970756","https://openalex.org/W1856219842","https://openalex.org/W1919876035","https://openalex.org/W1937279395","https://openalex.org/W1964577518","https://openalex.org/W1965460969","https://openalex.org/W1968413588","https://openalex.org/W1968870359","https://openalex.org/W1969254509","https://openalex.org/W1969670674","https://openalex.org/W1972954737","https://openalex.org/W1973110291","https://openalex.org/W1973667232","https://openalex.org/W1974548541","https://openalex.org/W1975222760","https://openalex.org/W1981536390","https://openalex.org/W1981577006","https://openalex.org/W1982196941","https://openalex.org/W1988061332","https://openalex.org/W1988595986","https://openalex.org/W1989503272","https://openalex.org/W1990710787","https://openalex.org/W1993817750","https://openalex.org/W1994174730","https://openalex.org/W1995050000","https://openalex.org/W2003835605","https://openalex.org/W2008788121","https://openalex.org/W2010694344","https://openalex.org/W2012342557","https://openalex.org/W2016074822","https://openalex.org/W2016377588","https://openalex.org/W2016451527","https://openalex.org/W2016561280","https://openalex.org/W2016752635","https://openalex.org/W2018102069","https://openalex.org/W2018497069","https://openalex.org/W2022298315","https://openalex.org/W2025923684","https://openalex.org/W2027234495","https://openalex.org/W2030437653","https://openalex.org/W2031663630","https://openalex.org/W2032420027","https://openalex.org/W2032704178","https://openalex.org/W2036722029","https://openalex.org/W2041168832","https://openalex.org/W2044213177","https://openalex.org/W2045547646","https://openalex.org/W2046762542","https://openalex.org/W2047889920","https://openalex.org/W2048001068","https://openalex.org/W2048788512","https://openalex.org/W2049106070","https://openalex.org/W2050747240","https://openalex.org/W2052531396","https://openalex.org/W2053484261","https://openalex.org/W2053653317","https://openalex.org/W2054670192","https://openalex.org/W2058061694","https://openalex.org/W2059067204","https://openalex.org/W2062412316","https://openalex.org/W2065669781","https://openalex.org/W2065814858","https://openalex.org/W2067297267","https://openalex.org/W2069957353","https://openalex.org/W2075476642","https://openalex.org/W2078355959","https://openalex.org/W2078775934","https://openalex.org/W2083843630","https://openalex.org/W2087453257","https://openalex.org/W2089505300","https://openalex.org/W2090118779","https://openalex.org/W2091369161","https://openalex.org/W2091646456","https://openalex.org/W2093957069","https://openalex.org/W2095812087","https://openalex.org/W2101867409","https://openalex.org/W2107828561","https://openalex.org/W2108053439","https://openalex.org/W2117762233","https://openalex.org/W2119363390","https://openalex.org/W2121185548","https://openalex.org/W2122205716","https://openalex.org/W2125303036","https://openalex.org/W2126294842","https://openalex.org/W2129316490","https://openalex.org/W2129393517","https://openalex.org/W2133783666","https://openalex.org/W2137072892","https://openalex.org/W2143443998","https://openalex.org/W2144948679","https://openalex.org/W2146817077","https://openalex.org/W2152211203","https://openalex.org/W2152902369","https://openalex.org/W2160383672","https://openalex.org/W2164191131","https://openalex.org/W2164479864","https://openalex.org/W2211317965","https://openalex.org/W2322141830","https://openalex.org/W4252682554"],"related_works":["https://openalex.org/W2245999762","https://openalex.org/W2247340604","https://openalex.org/W2522590786","https://openalex.org/W2053476031","https://openalex.org/W2295048324","https://openalex.org/W3148159410","https://openalex.org/W7024007745","https://openalex.org/W7021079141","https://openalex.org/W7030056270","https://openalex.org/W2223040168"],"abstract_inverted_index":{"The":[0],"combined":[1],"use":[2],"of":[3,37,46,90,93,122,135,149,256,278],"mixed":[4],"microbial":[5],"cultures":[6],"(MMC)":[7],"and":[8,20,63,95,114,129,173,220,238,246,259,284],"fermented":[9,71],"feedstock":[10],"as":[11,144,262,264],"substrate":[12,112],"may":[13],"significantly":[14],"decrease":[15],"polyhydroxyalkanoates":[16],"(PHA)":[17],"production":[18,67,97,116,287],"costs":[19],"make":[21,49],"them":[22],"more":[23,52],"competitive":[24],"in":[25,76,181,204],"relation":[26],"to":[27,48,65,86,105,110,126,153,164,187,194,252,265,274,282],"conventional":[28],"petroleum-based":[29],"polymers.":[30],"However,":[31],"there":[32],"still":[33],"exists":[34],"a":[35,82,123,150,189,227,240],"lack":[36],"knowledge":[38],"at":[39],"metabolic":[40,83,101,106,147,229],"level":[41],"that":[42],"limits":[43],"the":[44,88,119,127,133,136,145,170,196,200,205,217,235,254,257,267,276],"development":[45],"strategies":[47],"this":[50,55,154,271],"process":[51,258],"effective.":[53],"In":[54],"thesis,":[56],"system":[57],"biology":[58],"computational":[59,280],"tools":[60,281],"were":[61,210,221],"developed":[62],"applied":[64,104],"PHA":[66,96,115,171,247,286],"by":[68,156,216,288],"MMC":[69,151],"from":[70,179],"sugar":[72],"cane":[73],"molasses,":[74],"rich":[75],"volatile":[77],"fatty":[78],"acids":[79],"(VFA).":[80],"Firstly,":[81],"network":[84,102],"able":[85,193],"describe":[87,111],"uptake":[89,113,245],"complex":[91],"mixtures":[92],"VFA":[94,244],"was":[98,103,142,185,231],"defined.":[99],"This":[100,161,249],"flux":[107,157,197],"analysis":[108,159],"(MFA)":[109],"fluxes":[117,141,172],"over":[118],"enrichment":[120],"time":[121],"culture":[124],"submitted":[125],"feast":[128],"famine":[130],"regimen.":[131],"Then,":[132],"minimization":[134],"tricarboxylic":[137],"acid":[138],"cycle":[139],"(TCA)":[140],"identified":[143,203],"key":[146],"objective":[148],"subjected":[152],"regimen":[155],"balance":[158],"(FBA).":[160],"model":[162,192,230,250],"enabled":[163,273],"predict,":[165],"with":[166,223],"an":[167],"acceptable":[168],"accuracy,":[169],"biopolymer":[174],"composition.":[175],"Subsequently,":[176],"data":[177],"gathered":[178],"microautoradiography-fluorescence":[180],"situ":[182],"hybridization":[183],"(MAR-FISH)":[184],"used":[186],"develop":[188],"segregated":[190],"FBA":[191,219],"predict":[195],"distribution":[198],"for":[199,243],"three":[201],"populations":[202],"enriched":[206],"culture.":[207],"These":[208],"results":[209],"slightly":[211],"better":[212],"than":[213],"those":[214],"obtained":[215],"non-segregated":[218],"consistent":[222],"MFA":[224],"results.":[225,269],"Finally,":[226],"dynamic":[228],"proposed":[232],"based":[233],"on":[234,239],"previous":[236,268],"models":[237],"regulatory":[241,260],"factor":[242,261],"production.":[248],"allowed":[251],"identify":[253],"dynamics":[255],"well":[263],"validate":[266],"Globally,":[270],"thesis":[272],"demonstrate":[275],"potential":[277],"using":[279],"understand":[283],"optimize":[285],"MMC.":[289]},"counts_by_year":[],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2016-06-24T00:00:00"}