{"id":"https://openalex.org/W2182376660","doi":"https://doi.org/10.1242/jcs.110.7.911","title":"Defining the essential functional regions of the nucleoporin Nup145p","display_name":"Defining the essential functional regions of the nucleoporin Nup145p","publication_year":1997,"publication_date":"1997-04-01","ids":{"openalex":"https://openalex.org/W2182376660","doi":"https://doi.org/10.1242/jcs.110.7.911","mag":"2182376660","pmid":"https://pubmed.ncbi.nlm.nih.gov/9133678"},"language":"en","primary_location":{"id":"doi:10.1242/jcs.110.7.911","doi":"https://doi.org/10.1242/jcs.110.7.911","is_oa":false,"landing_page_url":"https://doi.org/10.1242/jcs.110.7.911","pdf_url":null,"source":{"id":"https://openalex.org/S5677253","display_name":"Journal of Cell Science","issn_l":"0021-9533","issn":["0021-9533","1477-9137"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311847","host_organization_name":"The Company of Biologists","host_organization_lineage":["https://openalex.org/P4310311847"],"host_organization_lineage_names":["The Company of Biologists"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Cell Science","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5027195984","display_name":"Jennifer L. T. Emtage","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I204465549","display_name":"Washington University in St. Louis","ror":"https://ror.org/01yc7t268","country_code":"US","type":"education","lineage":["https://openalex.org/I204465549"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jennifer L. T. Emtage","raw_affiliation_strings":["Washington University School of Medicine Department of Cell Biology and Physiology , , 660 S. Euclid Avenue, St Louis, MO 63110, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Washington University School of Medicine Department of Cell Biology and Physiology , , 660 S. Euclid Avenue, St Louis, MO 63110, USA","institution_ids":["https://openalex.org/I204465549"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5044632508","display_name":"Mirella Bucci","orcid":"https://orcid.org/0000-0003-1654-3758","observed_orcids":["https://orcid.org/0000-0003-1654-3758"]},"institutions":[{"id":"https://openalex.org/I204465549","display_name":"Washington University in St. Louis","ror":"https://ror.org/01yc7t268","country_code":"US","type":"education","lineage":["https://openalex.org/I204465549"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mirella Bucci","raw_affiliation_strings":["Washington University School of Medicine Department of Cell Biology and Physiology , , 660 S. Euclid Avenue, St Louis, MO 63110, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Washington University School of Medicine Department of Cell Biology and Physiology , , 660 S. Euclid Avenue, St Louis, MO 63110, USA","institution_ids":["https://openalex.org/I204465549"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023560340","display_name":"Janis L. Watkins","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I204465549","display_name":"Washington University in St. Louis","ror":"https://ror.org/01yc7t268","country_code":"US","type":"education","lineage":["https://openalex.org/I204465549"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Janis L. Watkins","raw_affiliation_strings":["Washington University School of Medicine Department of Cell Biology and Physiology , , 660 S. Euclid Avenue, St Louis, MO 63110, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Washington University School of Medicine Department of Cell Biology and Physiology , , 660 S. Euclid Avenue, St Louis, MO 63110, USA","institution_ids":["https://openalex.org/I204465549"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5017446481","display_name":"Susan R. Wente","orcid":"https://orcid.org/0000-0002-6240-548X","observed_orcids":["https://orcid.org/0000-0002-6240-548X"]},"institutions":[{"id":"https://openalex.org/I204465549","display_name":"Washington University in St. Louis","ror":"https://ror.org/01yc7t268","country_code":"US","type":"education","lineage":["https://openalex.org/I204465549"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Susan R. Wente","raw_affiliation_strings":["Washington University School of Medicine Department of Cell Biology and Physiology , , 660 S. Euclid Avenue, St Louis, MO 63110, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Washington University School of Medicine Department of Cell Biology and Physiology , , 660 S. Euclid Avenue, St Louis, MO 63110, USA","institution_ids":["https://openalex.org/I204465549"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I204465549"],"apc_list":null,"apc_paid":null,"fwci":1.6593,"has_fulltext":false,"cited_by_count":40,"citation_normalized_percentile":{"value":0.84380647,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"110","issue":"7","first_page":"911","last_page":"925"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11841","display_name":"Nuclear Structure and Function","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11841","display_name":"Nuclear Structure and Function","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10222","display_name":"Genomics and Chromatin Dynamics","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/nucleoporins","display_name":"nucleoporins","score":0.9240000247955322},{"id":"https://openalex.org/keywords/nuclear-pore-complex","display_name":"nuclear pore complex","score":0.824999988079071},{"id":"https://openalex.org/keywords/rna-export","display_name":"RNA export","score":0.8420000076293945},{"id":"https://openalex.org/keywords/temperature-sensitive-mutants","display_name":"temperature-sensitive mutants","score":0.8299999833106995},{"id":"https://openalex.org/keywords/functional-domains","display_name":"functional domains","score":0.5860000252723694},{"id":"https://openalex.org/keywords/n-terminal-domain","display_name":"N-terminal domain","score":0.6790000200271606},{"id":"https://openalex.org/keywords/c-terminal-domain","display_name":"C-terminal domain","score":0.8289999961853027},{"id":"https://openalex.org/keywords/nuclear-envelope","display_name":"nuclear envelope","score":0.6150000095367432},{"id":"https://openalex.org/keywords/cleavage-site","display_name":"cleavage site","score":0.6959999799728394},{"id":"https://openalex.org/keywords/proteolytic-cleavage","display_name":"proteolytic cleavage","score":0.6340000033378601},{"id":"https://openalex.org/keywords/saccharomyces-cerevisiae","display_name":"Saccharomyces cerevisiae","score":0.7900000214576721}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.9566607475280762},{"id":"https://openalex.org/C68556195","wikidata":"https://www.wikidata.org/wiki/Q3571630","display_name":"Nucleoporin","level":5,"score":0.8011662364006042},{"id":"https://openalex.org/C78458016","wikidata":"https://www.wikidata.org/wiki/Q840400","display_name":"Evolutionary biology","level":1,"score":0.4044968783855438},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.17893221974372864},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.06010642647743225},{"id":"https://openalex.org/C59157529","wikidata":"https://www.wikidata.org/wiki/Q994741","display_name":"Nuclear transport","level":4,"score":0.0552942156791687},{"id":"https://openalex.org/C2780114586","wikidata":"https://www.wikidata.org/wiki/Q40260","display_name":"Cell nucleus","level":3,"score":0.05133169889450073}],"mesh":[{"descriptor_ui":"D005656","descriptor_name":"Fungal Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D005656","descriptor_name":"Fungal Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D008854","descriptor_name":"Microscopy, Electron","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015003","descriptor_name":"Yeasts","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D015003","descriptor_name":"Yeasts","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D016601","descriptor_name":"RNA-Binding Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D016601","descriptor_name":"RNA-Binding Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D017403","descriptor_name":"In Situ Hybridization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D028861","descriptor_name":"Nuclear Pore Complex Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D029701","descriptor_name":"Saccharomyces cerevisiae Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1242/jcs.110.7.911","doi":"https://doi.org/10.1242/jcs.110.7.911","is_oa":false,"landing_page_url":"https://doi.org/10.1242/jcs.110.7.911","pdf_url":null,"source":{"id":"https://openalex.org/S5677253","display_name":"Journal of Cell Science","issn_l":"0021-9533","issn":["0021-9533","1477-9137"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311847","host_organization_name":"The Company of Biologists","host_organization_lineage":["https://openalex.org/P4310311847"],"host_organization_lineage_names":["The Company of Biologists"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Cell Science","raw_type":"journal-article"},{"id":"pmid:9133678","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9133678","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of cell science","raw_type":"Journal Article"},{"id":"pmh:oai:open-archive.highwire.org:joces:110/7/911","doi":null,"is_oa":false,"landing_page_url":"http://jcs.biologists.org/cgi/content/short/110/7/911","pdf_url":null,"source":{"id":"https://openalex.org/S4406923041","display_name":"HighWire Press Open Archive","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"TEXT"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1335552996","display_name":null,"funder_award_id":"R01 GM51219-02","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G6163701232","display_name":"Molecular Analysis of Nuclear Pore Complex Function","funder_award_id":"R01GM051219","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":45,"referenced_works":["https://openalex.org/W308604711","https://openalex.org/W1490035592","https://openalex.org/W1515930873","https://openalex.org/W1522668252","https://openalex.org/W1526043578","https://openalex.org/W1559083131","https://openalex.org/W1600651336","https://openalex.org/W1963846953","https://openalex.org/W1967890631","https://openalex.org/W1968676663","https://openalex.org/W1970705112","https://openalex.org/W1974905046","https://openalex.org/W1980073547","https://openalex.org/W1989308870","https://openalex.org/W1989650768","https://openalex.org/W1994390636","https://openalex.org/W2005579644","https://openalex.org/W2008267067","https://openalex.org/W2020803942","https://openalex.org/W2026117440","https://openalex.org/W2029912871","https://openalex.org/W2031196013","https://openalex.org/W2042015691","https://openalex.org/W2047182427","https://openalex.org/W2051991747","https://openalex.org/W2053416801","https://openalex.org/W2057123629","https://openalex.org/W2057493866","https://openalex.org/W2060403740","https://openalex.org/W2075791686","https://openalex.org/W2077358363","https://openalex.org/W2083496879","https://openalex.org/W2085304899","https://openalex.org/W2094733358","https://openalex.org/W2098620367","https://openalex.org/W2115109190","https://openalex.org/W2144634347","https://openalex.org/W2148058331","https://openalex.org/W2152993287","https://openalex.org/W2155287673","https://openalex.org/W2159293322","https://openalex.org/W2162882160","https://openalex.org/W2164405703","https://openalex.org/W2169333620","https://openalex.org/W2314225431"],"related_works":["https://openalex.org/W2016865656","https://openalex.org/W2119470870","https://openalex.org/W1994679382","https://openalex.org/W2114187223","https://openalex.org/W7095774447","https://openalex.org/W2031196013","https://openalex.org/W308604711","https://openalex.org/W2071824366","https://openalex.org/W2416313278","https://openalex.org/W2098620367"],"abstract_inverted_index":{"Studies":[0],"of":[1,22,29,39,46,83,90,101,104,154,174],"the":[2,36,52,56,69,72,91,126,200,235],"essential":[3,109],"nucleoporin":[4],"Nup145p":[5,102,148,194,201,206],"have":[6,42,145],"shown":[7],"that":[8,20,64,97,147,164,179],"its":[9,23],"depletion":[10],"is":[11,108,149,190],"coincident":[12],"with":[13],"a":[14,44,133,139,170,197,211,224],"block":[15],"in":[16,27,60,68,87,167,210,232],"RNA":[17,67],"export":[18],"and":[19,55,157],"deletion":[21,173],"amino-terminal":[24],"domain":[25],"results":[26,95],"clustering":[28,82],"nuclear":[30,84,92],"pore":[31,85],"complexes.":[32],"To":[33],"further":[34],"define":[35],"functional":[37],"domains":[38],"Nup145p,":[40],"we":[41],"characterized":[43],"panel":[45],"nup145":[47],"mutants.":[48],"Deletions":[49],"from":[50,227],"both":[51],"amino":[53,105,176,228],"terminus":[54,58],"carboxy":[57],"resulted":[59],"temperature":[61],"sensitive":[62],"mutants":[63,78],"accumulated":[65],"polyadenylated":[66],"nucleus":[70],"at":[71,122],"non-permissive":[73],"temperature.":[74],"In":[75],"addition,":[76],"these":[77],"also":[79],"displayed":[80],"constitutive":[81],"complexes":[86],"localized":[88],"patches":[89],"envelope.":[93],"These":[94],"suggested":[96,146],"an":[98],"internal":[99,172],"region":[100,115,127,226],"consisting":[103],"acids":[106],"593-893":[107],"for":[110,193,205,218],"function.":[111,195],"Accordingly,":[112],"when":[113,136],"this":[114],"was":[116,119,129,208,215],"deleted,":[117],"growth":[118],"not":[120,191,216],"supported":[121],"any":[123],"temperature,":[124],"whereas":[125],"alone":[128],"able":[130],"to":[131,234],"complement":[132],"null":[134],"mutation":[135],"expressed":[137],"on":[138,185],"high":[140],"copy":[141],"plasmid.":[142],"Previous":[143],"studies":[144],"cleaved":[150],"into":[151],"two":[152],"polypeptides":[153],"approximately":[155],"65":[156],"80":[158],"kDa.":[159],"Interestingly,":[160],"our":[161],"experiments":[162],"suggest":[163],"cleavage":[165,181,189,202,207,236],"occurs":[166],"vivo.":[168],"However,":[169],"small":[171],"17":[175],"acid":[177,229],"residues":[178,230],"abolished":[180],"had":[182],"no":[183],"effect":[184],"cell":[186],"growth.":[187],"Therefore,":[188],"necessary":[192],"When":[196],"sequence":[198],"harboring":[199],"site":[203],"required":[204],"inserted":[209],"chimeric":[212],"protein,":[213],"it":[214],"sufficient":[217],"mediating":[219],"cleavage.":[220],"Cleavage":[221],"likely":[222],"requires":[223],"second":[225],"247-524":[231],"addition":[233],"site.":[237]},"counts_by_year":[{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
