{"id":"https://openalex.org/W2181500752","doi":null,"title":"Protection of a restriction enzyme from heat inactivation by [alpha]-crystallin.","display_name":"Protection of a restriction enzyme from heat inactivation by [alpha]-crystallin.","publication_year":1998,"publication_date":"1998-12-15","ids":{"openalex":"https://openalex.org/W2181500752","mag":"2181500752","pmid":"https://pubmed.ncbi.nlm.nih.gov/9873067"},"language":"en","primary_location":{"id":"pmid:9873067","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9873067","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular vision","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5103401798","display_name":"John F. Hess","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I84218800","display_name":"University of California, Davis","ror":"https://ror.org/05rrcem69","country_code":"US","type":"education","lineage":["https://openalex.org/I84218800"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J F Hess","raw_affiliation_strings":["Department of Cell Biology and Human Anatomy, School of Medicine, University of California, Davis, CA 95616-8643, USA. jfhess@ucdavis.edu","Department of Cell Biology and Human Anatomy, School of Medicine, University of California, Davis, CA 95616-8643, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology and Human Anatomy, School of Medicine, University of California, Davis, CA 95616-8643, USA. jfhess@ucdavis.edu","institution_ids":["https://openalex.org/I84218800"]},{"raw_affiliation_string":"Department of Cell Biology and Human Anatomy, School of Medicine, University of California, Davis, CA 95616-8643, USA","institution_ids":["https://openalex.org/I84218800"]}]},{"author_position":"last","author":{"id":null,"display_name":"P G FitzGerald","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"P G FitzGerald","raw_affiliation_strings":["Cell Biology and Human Anatomy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cell Biology and Human Anatomy","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.1938,"has_fulltext":false,"cited_by_count":29,"citation_normalized_percentile":{"value":0.38950736,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":94},"biblio":{"volume":"4","issue":null,"first_page":"29","last_page":"29"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10934","display_name":"Heat shock proteins research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10934","display_name":"Heat shock proteins research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/alpha-crystallin","display_name":"alpha-crystallin","score":0.996999979019165},{"id":"https://openalex.org/keywords/chaperone-activity","display_name":"chaperone activity","score":0.7229999899864197},{"id":"https://openalex.org/keywords/thermal-inactivation","display_name":"thermal inactivation","score":0.9049999713897705},{"id":"https://openalex.org/keywords/thermal-protection","display_name":"thermal protection","score":0.8539999723434448},{"id":"https://openalex.org/keywords/protein-denaturation","display_name":"protein denaturation","score":0.6830000281333923},{"id":"https://openalex.org/keywords/enzyme-protection","display_name":"enzyme protection","score":0.41999998688697815},{"id":"https://openalex.org/keywords/plasmid-dna-cleavage","display_name":"plasmid DNA cleavage","score":0.7229999899864197}],"concepts":[{"id":"https://openalex.org/C2776907736","wikidata":"https://www.wikidata.org/wiki/Q408634","display_name":"Ethidium bromide","level":3,"score":0.6598173379898071},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5526830554008484},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5480437874794006},{"id":"https://openalex.org/C7263354","wikidata":"https://www.wikidata.org/wiki/Q2992167","display_name":"Crystallin","level":2,"score":0.5462493896484375},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5118178725242615},{"id":"https://openalex.org/C128319531","wikidata":"https://www.wikidata.org/wiki/Q219715","display_name":"Restriction enzyme","level":3,"score":0.503292977809906},{"id":"https://openalex.org/C2778271344","wikidata":"https://www.wikidata.org/wiki/Q903395","display_name":"Agarose gel electrophoresis","level":3,"score":0.5011975765228271},{"id":"https://openalex.org/C2778476535","wikidata":"https://www.wikidata.org/wiki/Q390697","display_name":"Agarose","level":2,"score":0.49011024832725525},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.47671717405319214},{"id":"https://openalex.org/C116084860","wikidata":"https://www.wikidata.org/wiki/Q48255","display_name":"Gel electrophoresis","level":2,"score":0.47410517930984497},{"id":"https://openalex.org/C2776923230","wikidata":"https://www.wikidata.org/wiki/Q16955798","display_name":"Denaturation (fissile materials)","level":2,"score":0.4639441967010498},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4378047287464142},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3813149631023407},{"id":"https://openalex.org/C13965031","wikidata":"https://www.wikidata.org/wiki/Q243545","display_name":"Nuclear chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003459","descriptor_name":"Crystallins","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D006358","descriptor_name":"Hot Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012710","descriptor_name":"Serum Albumin, Bovine","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D015252","descriptor_name":"Deoxyribonucleases, Type II Site-Specific","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D018832","descriptor_name":"Molecular Chaperones","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true}],"locations_count":2,"locations":[{"id":"pmid:9873067","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9873067","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular vision","raw_type":"Journal Article"},{"id":"mag:2181500752","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9873067","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":23,"referenced_works":["https://openalex.org/W389589487","https://openalex.org/W654116564","https://openalex.org/W1484534097","https://openalex.org/W1546877697","https://openalex.org/W1595514667","https://openalex.org/W1671787304","https://openalex.org/W1969752714","https://openalex.org/W1969929760","https://openalex.org/W1978545193","https://openalex.org/W1988230012","https://openalex.org/W1994916958","https://openalex.org/W2044031095","https://openalex.org/W2053797571","https://openalex.org/W2065590201","https://openalex.org/W2066897193","https://openalex.org/W2076381430","https://openalex.org/W2076521338","https://openalex.org/W2082323179","https://openalex.org/W2089816877","https://openalex.org/W2093473585","https://openalex.org/W2115807699","https://openalex.org/W2119405709","https://openalex.org/W2409937641"],"related_works":["https://openalex.org/W1691117","https://openalex.org/W2120728198","https://openalex.org/W2157365525","https://openalex.org/W1969929760","https://openalex.org/W2276906307","https://openalex.org/W1541400167","https://openalex.org/W2065388396","https://openalex.org/W2358887540","https://openalex.org/W1994916958","https://openalex.org/W2290403944"],"abstract_inverted_index":{"PURPOSE:":[0],"To":[1],"determine":[2],"whether":[3],"the":[4,27,38,42,78,90,126],"chaperone":[5,111],"activity":[6,40,112],"of":[7,31,41,50,55,65,74,80,92,100,106,116,121,135],"human":[8],"alpha-crystallin":[9,81,93,101,117,136],"can":[10],"protect":[11],"a":[12,103],"restriction":[13,20],"enzyme":[14,21],"from":[15,128],"heat":[16,36,43],"inactivation.":[17,84],"METHODS:":[18],"The":[19,53,110],"Nde":[22,75,86],"I":[23,76,87],"was":[24,46,57],"heated":[25,88],"in":[26,77,83,89],"presence":[28,91],"or":[29],"absence":[30,79],"purified":[32],"bovine":[33],"alpha-crystallin.":[34],"Following":[35],"treatment,":[37],"enzymatic":[39,122,139],"treated":[44],"samples":[45],"assayed":[47],"by":[48,59,68],"cleavage":[49],"plasmid":[51],"DNA.":[52],"extent":[54],"digestion":[56],"monitored":[58],"agarose":[60],"gel":[61],"electrophoresis":[62],"and":[63,113],"visualization":[64],"DNA":[66],"fragments":[67],"ethidium":[69],"bromide":[70],"staining.":[71],"RESULTS:":[72],"Heating":[73],"resulted":[82],"However,":[85],"remained":[94],"active.":[95],"Furthermore,":[96],"an":[97],"increased":[98],"amount":[99],"provided":[102],"longer":[104],"period":[105],"thermal":[107],"protection.":[108],"CONCLUSIONS:":[109],"thermo-protective":[114],"effect":[115],"extend":[118],"to":[119],"protection":[120,127],"activity,":[123],"not":[124],"merely":[125],"thermally":[129],"induced":[130],"aggregation/denaturation.":[131],"In":[132],"addition,":[133],"inclusion":[134],"during":[137],"some":[138],"reactions":[140],"may":[141],"be":[142],"beneficial.":[143]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}