{"id":"https://openalex.org/W2174357263","doi":"https://doi.org/10.1152/ajplung.1990.259.6.l415","title":"A novel epithelial cell from neonatal rat lung: isolation and differentiated phenotype","display_name":"A novel epithelial cell from neonatal rat lung: isolation and differentiated phenotype","publication_year":1990,"publication_date":"1990-12-01","ids":{"openalex":"https://openalex.org/W2174357263","doi":"https://doi.org/10.1152/ajplung.1990.259.6.l415","mag":"2174357263","pmid":"https://pubmed.ncbi.nlm.nih.gov/2260675"},"language":"en","primary_location":{"id":"doi:10.1152/ajplung.1990.259.6.l415","doi":"https://doi.org/10.1152/ajplung.1990.259.6.l415","is_oa":false,"landing_page_url":"https://doi.org/10.1152/ajplung.1990.259.6.l415","pdf_url":null,"source":{"id":"https://openalex.org/S47245711","display_name":"American Journal of Physiology-Lung Cellular and Molecular Physiology","issn_l":"1040-0605","issn":["1040-0605","1522-1504"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320261","host_organization_name":"American Physical Society","host_organization_lineage":["https://openalex.org/P4310320261"],"host_organization_lineage_names":["American Physical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"American Journal of Physiology-Lung Cellular and Molecular Physiology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5088376104","display_name":"P. E. Roberts","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I183934855","display_name":"Genentech","ror":"https://ror.org/04gndp242","country_code":"US","type":"company","lineage":["https://openalex.org/I118019719","https://openalex.org/I129302853","https://openalex.org/I183934855"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"P. E. Roberts","raw_affiliation_strings":["Department of Cell Biology, Genetech, South San Francisco, California 94080"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology, Genetech, South San Francisco, California 94080","institution_ids":["https://openalex.org/I183934855"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109044726","display_name":"David M. Phillips","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I183934855","display_name":"Genentech","ror":"https://ror.org/04gndp242","country_code":"US","type":"company","lineage":["https://openalex.org/I118019719","https://openalex.org/I129302853","https://openalex.org/I183934855"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"D. M. Phillips","raw_affiliation_strings":["Department of Cell Biology, Genetech, South San Francisco, California 94080"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology, Genetech, South San Francisco, California 94080","institution_ids":["https://openalex.org/I183934855"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5112824383","display_name":"Jennie P. Mather","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I183934855","display_name":"Genentech","ror":"https://ror.org/04gndp242","country_code":"US","type":"company","lineage":["https://openalex.org/I118019719","https://openalex.org/I129302853","https://openalex.org/I183934855"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J. P. Mather","raw_affiliation_strings":["Department of Cell Biology, Genetech, South San Francisco, California 94080"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology, Genetech, South San Francisco, California 94080","institution_ids":["https://openalex.org/I183934855"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I183934855"],"apc_list":null,"apc_paid":null,"fwci":1.0832,"has_fulltext":false,"cited_by_count":26,"citation_normalized_percentile":{"value":0.8014739,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"259","issue":"6","first_page":"L415","last_page":"L425"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10549","display_name":"Neonatal Respiratory Health Research","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/2740","display_name":"Pulmonary and Respiratory Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10549","display_name":"Neonatal Respiratory Health Research","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/2740","display_name":"Pulmonary and Respiratory Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11189","display_name":"Transplantation: Methods and Outcomes","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/2746","display_name":"Surgery"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10051","display_name":"Asthma and respiratory diseases","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/neonatal-rat-lung","display_name":"neonatal rat lung","score":0.7950000166893005},{"id":"https://openalex.org/keywords/bronchiolar-epithelial-cells","display_name":"bronchiolar epithelial cells","score":0.9079999923706055},{"id":"https://openalex.org/keywords/cell-line-establishment","display_name":"cell line establishment","score":0.7360000014305115},{"id":"https://openalex.org/keywords/serum-free-culture","display_name":"serum-free culture","score":0.6489999890327454},{"id":"https://openalex.org/keywords/defined-culture-medium","display_name":"defined culture medium","score":0.6010000109672546},{"id":"https://openalex.org/keywords/cortisol","display_name":"cortisol","score":0.8159999847412109},{"id":"https://openalex.org/keywords/forskolin","display_name":"forskolin","score":0.984000027179718},{"id":"https://openalex.org/keywords/retinoic-acid","display_name":"retinoic acid","score":0.8230000138282776},{"id":"https://openalex.org/keywords/keratinization","display_name":"keratinization","score":0.8510000109672546},{"id":"https://openalex.org/keywords/cell-proliferation","display_name":"cell proliferation","score":0.8420000076293945},{"id":"https://openalex.org/keywords/cell-differentiation","display_name":"cell differentiation","score":0.6660000085830688},{"id":"https://openalex.org/keywords/clonal-selection","display_name":"clonal selection","score":0.6499999761581421}],"concepts":[{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.6127270460128784},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5630589127540588},{"id":"https://openalex.org/C201750760","wikidata":"https://www.wikidata.org/wiki/Q1896218","display_name":"Progenitor cell","level":3,"score":0.5212327241897583},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.5098027586936951},{"id":"https://openalex.org/C22814914","wikidata":"https://www.wikidata.org/wiki/Q410473","display_name":"Transferrin","level":2,"score":0.4739163815975189},{"id":"https://openalex.org/C2781121885","wikidata":"https://www.wikidata.org/wiki/Q425517","display_name":"Retinoic acid","level":3,"score":0.4636335074901581},{"id":"https://openalex.org/C62112901","wikidata":"https://www.wikidata.org/wiki/Q189159","display_name":"Cell growth","level":2,"score":0.4581941068172455},{"id":"https://openalex.org/C148738053","wikidata":"https://www.wikidata.org/wiki/Q210861","display_name":"Cellular differentiation","level":3,"score":0.45674198865890503},{"id":"https://openalex.org/C189014844","wikidata":"https://www.wikidata.org/wiki/Q189118","display_name":"Cell type","level":3,"score":0.45081835985183716},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.44625338912010193},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.4417009949684143},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.43907102942466736},{"id":"https://openalex.org/C98648569","wikidata":"https://www.wikidata.org/wiki/Q5090513","display_name":"Chemically defined medium","level":3,"score":0.41546618938446045},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.39542484283447266},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.38903799653053284},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.363048791885376},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.2530788779258728},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.23067310452461243},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.10289266705513},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C99454951","wikidata":"https://www.wikidata.org/wiki/Q932068","display_name":"Environmental health","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000831","descriptor_name":"Animals, Newborn","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002454","descriptor_name":"Cell Differentiation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002455","descriptor_name":"Cell Division","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002469","descriptor_name":"Cell Separation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003470","descriptor_name":"Culture Media","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003598","descriptor_name":"Cytoskeletal Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D003896","descriptor_name":"Desmosomes","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D004847","descriptor_name":"Epithelial Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004848","descriptor_name":"Epithelium","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D005455","descriptor_name":"Fluorescent Antibody Technique","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008168","descriptor_name":"Lung","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008841","descriptor_name":"Actin Cytoskeleton","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D008854","descriptor_name":"Microscopy, Electron","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010641","descriptor_name":"Phenotype","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011919","descriptor_name":"Rats, Inbred Strains","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1152/ajplung.1990.259.6.l415","doi":"https://doi.org/10.1152/ajplung.1990.259.6.l415","is_oa":false,"landing_page_url":"https://doi.org/10.1152/ajplung.1990.259.6.l415","pdf_url":null,"source":{"id":"https://openalex.org/S47245711","display_name":"American Journal of Physiology-Lung Cellular and Molecular Physiology","issn_l":"1040-0605","issn":["1040-0605","1522-1504"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320261","host_organization_name":"American Physical Society","host_organization_lineage":["https://openalex.org/P4310320261"],"host_organization_lineage_names":["American Physical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"American Journal of Physiology-Lung Cellular and Molecular Physiology","raw_type":"journal-article"},{"id":"pmid:2260675","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2260675","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The American journal of physiology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.8048999905586243}],"sustainable_development_goals_aurora":[{"display_name":"Zero hunger","id":"https://metadata.un.org/sdg/2","score":0.6200000047683716}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1197234823","https://openalex.org/W2505790472","https://openalex.org/W2409947572","https://openalex.org/W2066078730","https://openalex.org/W2286127891","https://openalex.org/W2058428781","https://openalex.org/W3026485818","https://openalex.org/W2015163780","https://openalex.org/W6889280962","https://openalex.org/W2019177713"],"abstract_inverted_index":{"A":[0,67],"novel":[1],"epithelial":[2,47,65],"cell":[3,48,59,99,157,178,197],"from":[4,41,199],"normal":[5],"neonatal":[6],"rat":[7],"lung":[8,201,235],"has":[9,61,71,85],"been":[10,37,72,86],"isolated,":[11],"established,":[12],"and":[13,74,91,132,159,180,202,231,236],"maintained":[14],"for":[15,76,97,207],"multiple":[16],"passages":[17],"in":[18,56,81,156,165,174,210,222,233,244],"the":[19,32,146,200,234,240],"absence":[20],"of":[21,31,63,138,141,170,177,183],"serum,":[22],"without":[23],"undergoing":[24],"crisis":[25],"or":[26],"senescence.":[27],"By":[28],"careful":[29],"manipulation":[30],"nutrition/hormonal":[33],"microenvironment,":[34],"we":[35],"have":[36,186],"able":[38],"to":[39,145,162,194,217,224,237],"select,":[40],"a":[42,45,151,160,188,213,219],"heterogeneous":[43],"population,":[44],"single":[46],"type":[49,60],"that":[50,190],"can":[51],"maintain":[52],"highly":[53],"differentiated":[54,205],"features":[55],"vitro.":[57,211],"This":[58],"characteristics":[62,206],"bronchiolar":[64],"cells.":[66],"clonal":[68],"line,":[69],"RL-65,":[70],"selected":[73],"observed":[75],"greater":[77,152],"than":[78,153],"2":[79],"yr":[80],"continuous":[82],"culture.":[83],"It":[84],"characterized":[87],"by":[88],"ultrastructural,":[89],"morphological,":[90],"biochemical":[92],"criteria.":[93],"The":[94,136,168],"basal":[95,148],"medium":[96,107,149],"this":[98],"line":[100],"is":[101],"Ham's":[102],"F12/Dulbecco's":[103],"modified":[104],"Eagle's":[105],"(DME)":[106],"plus":[108],"insulin":[109],"(1":[110],"micrograms/ml),":[111,115],"human":[112],"transferrin":[113],"(10":[114],"ethanolamine":[116],"(10(-4)":[117,120],"M),":[118,121,126,131],"phosphoethanolamine":[119],"selenium":[122],"(2.5":[123,128],"x":[124,129],"10(-8)":[125],"hydrocortisone":[127],"10(-7)":[130],"forskolin":[133],"(5":[134],"microM).":[135],"addition":[137,169],"150":[139],"micrograms/ml":[140],"bovine":[142],"pituitary":[143],"extract":[144],"defined":[147],"stimulates":[150],"10-fold":[154],"increase":[155,164],"number":[158],"50-":[161],"100-fold":[163],"thymidine":[166],"incorporation.":[167],"retinoic":[171],"acid":[172],"results":[173],"further":[175],"enhancement":[176],"growth":[179],"complete":[181],"inhibition":[182],"keratinization.":[184],"We":[185],"demonstrated":[187],"strategy":[189],"may":[191],"be":[192,218],"applicable":[193],"isolating":[195],"other":[196],"types":[198],"maintaining":[203],"their":[204],"long-term":[208],"culture":[209,214],"Such":[212],"system":[215],"promises":[216],"useful":[220],"model":[221],"which":[223],"study":[225],"cellular":[226],"events":[227],"associated":[228],"with":[229],"differentiation":[230],"proliferation":[232],"better":[238],"understand":[239],"molecular":[241],"mechanisms":[242],"involved":[243],"these":[245],"events.":[246]},"counts_by_year":[{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}