{"id":"https://openalex.org/W2174064394","doi":"https://doi.org/10.1637/0005-2086(2002)046[0570:eoilvg]2.0.co;2","title":"Expression of Infectious Laryngotracheitis Virus Glycoproteins in Escherichia coli and Their Application in Enzyme-Linked Immunosorbent Assay","display_name":"Expression of Infectious Laryngotracheitis Virus Glycoproteins in Escherichia coli and Their Application in Enzyme-Linked Immunosorbent Assay","publication_year":2002,"publication_date":"2002-07-01","ids":{"openalex":"https://openalex.org/W2174064394","doi":"https://doi.org/10.1637/0005-2086(2002)046[0570:eoilvg]2.0.co;2","mag":"2174064394","pmid":"https://pubmed.ncbi.nlm.nih.gov/12243520"},"language":"en","primary_location":{"id":"doi:10.1637/0005-2086(2002)046[0570:eoilvg]2.0.co;2","doi":"https://doi.org/10.1637/0005-2086(2002)046[0570:eoilvg]2.0.co;2","is_oa":false,"landing_page_url":"https://doi.org/10.1637/0005-2086(2002)046[0570:eoilvg]2.0.co;2","pdf_url":null,"source":{"id":"https://openalex.org/S68792902","display_name":"Avian Diseases","issn_l":"0005-2086","issn":["0005-2086","1938-4351"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4320800605","host_organization_name":"American Association of Avian Pathologists","host_organization_lineage":["https://openalex.org/P4320800605"],"host_organization_lineage_names":["American Association of Avian Pathologists"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Avian Diseases","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5113690503","display_name":"Poa\u2010Chun Chang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I162838928","display_name":"National Chung Hsing University","ror":"https://ror.org/05vn3ca78","country_code":"TW","type":"education","lineage":["https://openalex.org/I162838928"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"Poa-Chun Chang","raw_affiliation_strings":["Institute of Veterinary Microbiology, National Chung Hsing University, Taichung, Taiwan","aInstitute of Veterinary Microbiology, National Chung Hsing University, Taichung 402, Taiwan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Veterinary Microbiology, National Chung Hsing University, Taichung, Taiwan","institution_ids":["https://openalex.org/I162838928"]},{"raw_affiliation_string":"aInstitute of Veterinary Microbiology, National Chung Hsing University, Taichung 402, Taiwan","institution_ids":["https://openalex.org/I162838928"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5039445614","display_name":"Kwan-Ting Chen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I162838928","display_name":"National Chung Hsing University","ror":"https://ror.org/05vn3ca78","country_code":"TW","type":"education","lineage":["https://openalex.org/I162838928"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"Kwan-Ting Chen","raw_affiliation_strings":["aInstitute of Veterinary Microbiology, National Chung Hsing University, Taichung 402, Taiwan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"aInstitute of Veterinary Microbiology, National Chung Hsing University, Taichung 402, Taiwan","institution_ids":["https://openalex.org/I162838928"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108396013","display_name":"Jui\u2010Hung Shien","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I162838928","display_name":"National Chung Hsing University","ror":"https://ror.org/05vn3ca78","country_code":"TW","type":"education","lineage":["https://openalex.org/I162838928"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"Jui-Hung Shien","raw_affiliation_strings":["bDepartment of Veterinary Medicine, National Chung Hsing University, Taichung 402, Taiwan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"bDepartment of Veterinary Medicine, National Chung Hsing University, Taichung 402, Taiwan","institution_ids":["https://openalex.org/I162838928"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5113689158","display_name":"Happy K. Shieh","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I162838928","display_name":"National Chung Hsing University","ror":"https://ror.org/05vn3ca78","country_code":"TW","type":"education","lineage":["https://openalex.org/I162838928"]}],"countries":["TW"],"is_corresponding":true,"raw_author_name":"Happy K. Shieh","raw_affiliation_strings":["bDepartment of Veterinary Medicine, National Chung Hsing University, Taichung 402, Taiwan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"bDepartment of Veterinary Medicine, National Chung Hsing University, Taichung 402, Taiwan","institution_ids":["https://openalex.org/I162838928"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5113689158"],"corresponding_institution_ids":["https://openalex.org/I162838928"],"apc_list":null,"apc_paid":null,"fwci":0.585,"has_fulltext":false,"cited_by_count":22,"citation_normalized_percentile":{"value":0.68229913,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"46","issue":"3","first_page":"570","last_page":"580"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11495","display_name":"Animal Virus Infections Studies","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11495","display_name":"Animal Virus Infections Studies","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11135","display_name":"Virology and Viral Diseases","score":0.0010000000474974513,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11688","display_name":"Microbial infections and disease research","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/infectious-laryngotracheitis-virus","display_name":"infectious laryngotracheitis virus","score":1.0},{"id":"https://openalex.org/keywords/glycoproteins","display_name":"glycoproteins","score":0.7419999837875366},{"id":"https://openalex.org/keywords/ge","display_name":"Ge","score":0.640999972820282},{"id":"https://openalex.org/keywords/gp60","display_name":"gp60 gene","score":0.9919999837875366},{"id":"https://openalex.org/keywords/gc","display_name":"GC","score":0.9039999842643738},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.7929999828338623},{"id":"https://openalex.org/keywords/elisa-antigen","display_name":"ELISA antigen","score":0.828000009059906},{"id":"https://openalex.org/keywords/antibody-detection","display_name":"antibody detection","score":0.8029999732971191},{"id":"https://openalex.org/keywords/chickens","display_name":"chickens","score":0.4410000145435333},{"id":"https://openalex.org/keywords/marker-vaccine","display_name":"marker vaccine","score":0.7170000076293945}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.70138019323349},{"id":"https://openalex.org/C2779026604","wikidata":"https://www.wikidata.org/wiki/Q4774742","display_name":"Antigenicity","level":3,"score":0.6795194149017334},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.6691535115242004},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.5525408983230591},{"id":"https://openalex.org/C108625454","wikidata":"https://www.wikidata.org/wiki/Q187126","display_name":"Glycoprotein","level":2,"score":0.539516806602478},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.5257571339607239},{"id":"https://openalex.org/C91523082","wikidata":"https://www.wikidata.org/wiki/Q420496","display_name":"Antiserum","level":3,"score":0.5192987322807312},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.4786487817764282},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.4560548961162567},{"id":"https://openalex.org/C956191","wikidata":"https://www.wikidata.org/wiki/Q930144","display_name":"Polyclonal antibodies","level":3,"score":0.4549597501754761},{"id":"https://openalex.org/C123894998","wikidata":"https://www.wikidata.org/wiki/Q425158","display_name":"Fusion protein","level":4,"score":0.41224223375320435},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4024505913257599},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.33349794149398804},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.19618430733680725},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.13177958130836487},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.12994074821472168},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000914","descriptor_name":"Antibodies, Viral","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D000956","descriptor_name":"Antigens, Viral","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D000956","descriptor_name":"Antigens, Viral","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000956","descriptor_name":"Antigens, Viral","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D001352","descriptor_name":"Herpesvirus 1, Gallid","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001352","descriptor_name":"Herpesvirus 1, Gallid","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D001352","descriptor_name":"Herpesvirus 1, Gallid","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D002645","descriptor_name":"Chickens","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004797","descriptor_name":"Enzyme-Linked Immunosorbent Assay","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D004797","descriptor_name":"Enzyme-Linked Immunosorbent Assay","qualifier_ui":"Q000662","qualifier_name":"veterinary","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006023","descriptor_name":"Glycoproteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D006023","descriptor_name":"Glycoproteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D006023","descriptor_name":"Glycoproteins","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D006566","descriptor_name":"Herpesviridae Infections","qualifier_ui":"Q000175","qualifier_name":"diagnosis","is_major_topic":false},{"descriptor_ui":"D006566","descriptor_name":"Herpesviridae Infections","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D006566","descriptor_name":"Herpesviridae Infections","qualifier_ui":"Q000517","qualifier_name":"prevention & control","is_major_topic":false},{"descriptor_ui":"D006566","descriptor_name":"Herpesviridae Infections","qualifier_ui":"Q000662","qualifier_name":"veterinary","is_major_topic":true},{"descriptor_ui":"D011897","descriptor_name":"Random Allocation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D012680","descriptor_name":"Sensitivity and Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012995","descriptor_name":"Solubility","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013047","descriptor_name":"Specific Pathogen-Free Organisms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014611","descriptor_name":"Vaccination","qualifier_ui":"Q000662","qualifier_name":"veterinary","is_major_topic":false},{"descriptor_ui":"D015153","descriptor_name":"Blotting, Western","qualifier_ui":"Q000662","qualifier_name":"veterinary","is_major_topic":false},{"descriptor_ui":"D015967","descriptor_name":"Gene Expression Regulation, Viral","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000662","qualifier_name":"veterinary","is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1637/0005-2086(2002)046[0570:eoilvg]2.0.co;2","doi":"https://doi.org/10.1637/0005-2086(2002)046[0570:eoilvg]2.0.co;2","is_oa":false,"landing_page_url":"https://doi.org/10.1637/0005-2086(2002)046[0570:eoilvg]2.0.co;2","pdf_url":null,"source":{"id":"https://openalex.org/S68792902","display_name":"Avian Diseases","issn_l":"0005-2086","issn":["0005-2086","1938-4351"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4320800605","host_organization_name":"American Association of Avian Pathologists","host_organization_lineage":["https://openalex.org/P4320800605"],"host_organization_lineage_names":["American Association of Avian Pathologists"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Avian Diseases","raw_type":"journal-article"},{"id":"pmid:12243520","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12243520","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Avian diseases","raw_type":"Journal Article"},{"id":"pmh:10.1637/0005-2086(2002)046[0570:EOILVG]2.0.CO;2","doi":null,"is_oa":false,"landing_page_url":"http://www.bioone.org/doi/10.1637/0005-2086%282002%29046%5B0570%3AEOILVG%5D2.0.CO%3B2","pdf_url":null,"source":{"id":"https://openalex.org/S4306402358","display_name":"BioOne Complete (BioOne)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210111627","host_organization_name":"BioOne","host_organization_lineage":["https://openalex.org/I4210111627"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"text"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.8560000061988831,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":39,"referenced_works":["https://openalex.org/W82916198","https://openalex.org/W1504843606","https://openalex.org/W1964886508","https://openalex.org/W1964937340","https://openalex.org/W1969400866","https://openalex.org/W1972482405","https://openalex.org/W1972914784","https://openalex.org/W1974036835","https://openalex.org/W1974552028","https://openalex.org/W1981987422","https://openalex.org/W1982309523","https://openalex.org/W1992975423","https://openalex.org/W1994607148","https://openalex.org/W1996137016","https://openalex.org/W1999068352","https://openalex.org/W2000877603","https://openalex.org/W2001601416","https://openalex.org/W2004072334","https://openalex.org/W2005588525","https://openalex.org/W2011286523","https://openalex.org/W2012892224","https://openalex.org/W2016797201","https://openalex.org/W2018032284","https://openalex.org/W2022724574","https://openalex.org/W2027827997","https://openalex.org/W2029989019","https://openalex.org/W2038504429","https://openalex.org/W2062833070","https://openalex.org/W2081297600","https://openalex.org/W2095402167","https://openalex.org/W2100815241","https://openalex.org/W2128014934","https://openalex.org/W2146900298","https://openalex.org/W2149264081","https://openalex.org/W2164882148","https://openalex.org/W2334991330","https://openalex.org/W2394917973","https://openalex.org/W2406287958","https://openalex.org/W4391199178"],"related_works":["https://openalex.org/W2158680190","https://openalex.org/W1967440088","https://openalex.org/W256044449","https://openalex.org/W2949378663","https://openalex.org/W1989948802","https://openalex.org/W1506774736","https://openalex.org/W1992759008","https://openalex.org/W2376615831","https://openalex.org/W2128130418","https://openalex.org/W2316156146"],"abstract_inverted_index":{"Three":[0],"glycoproteins":[1],"of":[2,91,101,109,180,194],"infectious":[3,181],"laryngotracheitis":[4,182],"virus":[5],"(ILTV),":[6],"gC,":[7],"gE,":[8],"and":[9,34,53,66,74,93,121,137,154],"gp60,":[10],"were":[11,58,68],"expressed":[12],"in":[13,177,184],"Escherichia":[14],"coli":[15],"as":[16,31,40,77,158],"fusion":[17],"proteins":[18,28],"with":[19,45,98],"a":[20,71],"6-histidine":[21],"tag":[22],"at":[23],"their":[24,38],"amino":[25],"termini.":[26],"The":[27,63,88,116],"expressed,":[29],"designated":[30],"r-gC,":[32,57],"r-gp60,":[33],"r-gE,":[35,54],"all":[36],"retain":[37],"antigenicity,":[39],"revealed":[41],"by":[42,70],"Western":[43],"blot":[44],"chicken":[46],"antiserum":[47],"against":[48],"ILTV.":[49],"However,":[50],"only":[51],"r-gp60":[52,65,94,120,136,153,168],"but":[55],"not":[56],"found":[59],"to":[60],"be":[61],"soluble.":[62],"soluble":[64],"r-gE":[67,92,122,138,155,170],"purified":[69],"nickel":[72],"column":[73],"then":[75],"used":[76],"the":[78,99,178,185,188],"enzyme-linked":[79],"immunosorbent":[80],"assay":[81],"(ELISA)":[82],"antigen":[83,162],"for":[84,163],"detecting":[85,164],"ILTV-specific":[86,165],"antibodies.":[87,166],"diagnostic":[89],"potential":[90],"ELISA":[95,123,139,161,171],"was":[96],"assessed":[97],"use":[100],"sera":[102],"prepared":[103],"from":[104,129,146],"vaccinated":[105,126,143],"or":[106,113,169,190],"unvaccinated":[107,130,147],"chickens":[108,128],"either":[110],"specific-pathogen-free":[111],"(SPF)":[112],"field":[114,144],"origins.":[115],"result":[117,150],"shows":[118],"that":[119,152],"could":[124,140],"discriminate":[125,142],"SPF":[127],"ones":[131],"2":[132],"wk":[133],"postvaccination.":[134],"Moreover,":[135,167],"also":[141],"flocks":[145],"ones.":[148],"This":[149],"indicates":[151],"might":[156,172],"serve":[157],"an":[159,174],"alternative":[160],"play":[173],"important":[175],"role":[176],"eradication":[179],"(ILT)":[183],"future":[186],"when":[187],"gp60-":[189],"gE-deleted":[191],"marker":[192],"vaccine":[193],"ILT":[195],"is":[196],"available.":[197]},"counts_by_year":[{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":4},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}