{"id":"https://openalex.org/W2172773063","doi":"https://doi.org/10.1089/153623002753632011","title":"The Use of Nuclear Transfer to Produce Transgenic Pigs","display_name":"The Use of Nuclear Transfer to Produce Transgenic Pigs","publication_year":2002,"publication_date":"2002-03-01","ids":{"openalex":"https://openalex.org/W2172773063","doi":"https://doi.org/10.1089/153623002753632011","mag":"2172773063","pmid":"https://pubmed.ncbi.nlm.nih.gov/12006153"},"language":"en","primary_location":{"id":"doi:10.1089/153623002753632011","doi":"https://doi.org/10.1089/153623002753632011","is_oa":false,"landing_page_url":"https://doi.org/10.1089/153623002753632011","pdf_url":null,"source":{"id":"https://openalex.org/S136641558","display_name":"Cloning and Stem Cells","issn_l":"1536-2302","issn":["1536-2302","1557-7457"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320443","host_organization_name":"Mary Ann Liebert, Inc.","host_organization_lineage":["https://openalex.org/P4310320443","https://openalex.org/P4310320017"],"host_organization_lineage_names":["Mary Ann Liebert, Inc.","SAGE Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cloning and Stem Cells","raw_type":"journal-article"},"volume":null,"type":"review","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5015759451","display_name":"Zolt\u00e1n Mach\u00e1ty","orcid":"https://orcid.org/0000-0001-9021-1706","observed_orcids":["https://orcid.org/0000-0001-9021-1706"]},"institutions":[{"id":"https://openalex.org/I4210125492","display_name":"Alexion Pharmaceuticals (United States)","ror":"https://ror.org/031ywxc85","country_code":"US","type":"company","lineage":["https://openalex.org/I4210125492"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Zolt\u00e1n Mach\u00e1ty","raw_affiliation_strings":["Alexion Pharmaceuticals, Inc., Cheshire, Connecticut"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Alexion Pharmaceuticals, Inc., Cheshire, Connecticut","institution_ids":["https://openalex.org/I4210125492"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068281338","display_name":"K. R. Bondioli","orcid":"https://orcid.org/0000-0003-2757-2909","observed_orcids":["https://orcid.org/0000-0003-2757-2909"]},"institutions":[{"id":"https://openalex.org/I4210125492","display_name":"Alexion Pharmaceuticals (United States)","ror":"https://ror.org/031ywxc85","country_code":"US","type":"company","lineage":["https://openalex.org/I4210125492"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kenneth R. Bondioli","raw_affiliation_strings":["Alexion Pharmaceuticals, Inc., Cheshire, Connecticut"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Alexion Pharmaceuticals, Inc., Cheshire, Connecticut","institution_ids":["https://openalex.org/I4210125492"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074563323","display_name":"Jagdeece Ramsoondar","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210125492","display_name":"Alexion Pharmaceuticals (United States)","ror":"https://ror.org/031ywxc85","country_code":"US","type":"company","lineage":["https://openalex.org/I4210125492"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jagdeece J. Ramsoondar","raw_affiliation_strings":["Alexion Pharmaceuticals, Inc., Cheshire, Connecticut"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Alexion Pharmaceuticals, Inc., Cheshire, Connecticut","institution_ids":["https://openalex.org/I4210125492"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5009948579","display_name":"William L. Fodor","orcid":"https://orcid.org/0000-0001-8616-4425","observed_orcids":["https://orcid.org/0000-0001-8616-4425"]},"institutions":[{"id":"https://openalex.org/I4210125492","display_name":"Alexion Pharmaceuticals (United States)","ror":"https://ror.org/031ywxc85","country_code":"US","type":"company","lineage":["https://openalex.org/I4210125492"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"William L. Fodor","raw_affiliation_strings":["Alexion Pharmaceuticals, Inc., Cheshire, Connecticut"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Alexion Pharmaceuticals, Inc., Cheshire, Connecticut","institution_ids":["https://openalex.org/I4210125492"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I4210125492"],"apc_list":null,"apc_paid":null,"fwci":0.1932,"has_fulltext":false,"cited_by_count":18,"citation_normalized_percentile":{"value":0.21547502,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"4","issue":"1","first_page":"21","last_page":"27"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12929","display_name":"Animal Genetics and Reproduction","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12929","display_name":"Animal Genetics and Reproduction","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12654","display_name":"Xenotransplantation and immune response","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/2746","display_name":"Surgery"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/nuclear-transfer","display_name":"nuclear transfer","score":0.9769999980926514},{"id":"https://openalex.org/keywords/transgenic-pigs","display_name":"transgenic pigs","score":0.996999979019165},{"id":"https://openalex.org/keywords/homologous-recombination","display_name":"homologous recombination","score":0.9369999766349792},{"id":"https://openalex.org/keywords/xenotransplantation","display_name":"xenotransplantation","score":0.9200000166893005},{"id":"https://openalex.org/keywords/random-insertion","display_name":"random insertion","score":0.4230000078678131},{"id":"https://openalex.org/keywords/transgene-integration","display_name":"transgene integration","score":0.38100001215934753}],"concepts":[{"id":"https://openalex.org/C102744134","wikidata":"https://www.wikidata.org/wiki/Q22280388","display_name":"Homologous recombination","level":3,"score":0.7630263566970825},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.7574851512908936},{"id":"https://openalex.org/C2778442404","wikidata":"https://www.wikidata.org/wiki/Q55505","display_name":"Xenotransplantation","level":3,"score":0.7513970732688904},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6880651116371155},{"id":"https://openalex.org/C51456166","wikidata":"https://www.wikidata.org/wiki/Q182726","display_name":"Genetically modified organism","level":3,"score":0.5369557738304138},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.5338958501815796},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4515213370323181},{"id":"https://openalex.org/C64894306","wikidata":"https://www.wikidata.org/wiki/Q849622","display_name":"Homologous chromosome","level":3,"score":0.4406076967716217},{"id":"https://openalex.org/C112672928","wikidata":"https://www.wikidata.org/wiki/Q239526","display_name":"Offspring","level":3,"score":0.4393622875213623},{"id":"https://openalex.org/C38027674","wikidata":"https://www.wikidata.org/wiki/Q4994856","display_name":"Cre-Lox recombination","level":5,"score":0.4137188792228699},{"id":"https://openalex.org/C23837897","wikidata":"https://www.wikidata.org/wiki/Q159236","display_name":"Genetic engineering","level":3,"score":0.41234782338142395},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.405484139919281},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.3733227849006653},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.36206144094467163},{"id":"https://openalex.org/C141035611","wikidata":"https://www.wikidata.org/wiki/Q5532934","display_name":"Genetically modified mouse","level":4,"score":0.2944871783256531},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.22556301951408386},{"id":"https://openalex.org/C2911091166","wikidata":"https://www.wikidata.org/wiki/Q106419912","display_name":"Transplantation","level":2,"score":0.21713736653327942},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.08429545164108276},{"id":"https://openalex.org/C141071460","wikidata":"https://www.wikidata.org/wiki/Q40821","display_name":"Surgery","level":1,"score":0.0},{"id":"https://openalex.org/C2779234561","wikidata":"https://www.wikidata.org/wiki/Q11995","display_name":"Pregnancy","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004624","descriptor_name":"Embryo Transfer","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005821","descriptor_name":"Genetic Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008845","descriptor_name":"Microinjections","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011995","descriptor_name":"Recombination, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012190","descriptor_name":"Retroviridae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D013094","descriptor_name":"Spermatozoa","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":false},{"descriptor_ui":"D013552","descriptor_name":"Swine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014183","descriptor_name":"Transplantation, Heterologous","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D030801","descriptor_name":"Animals, Genetically Modified","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D053652","descriptor_name":"Nuclear Transfer Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1089/153623002753632011","doi":"https://doi.org/10.1089/153623002753632011","is_oa":false,"landing_page_url":"https://doi.org/10.1089/153623002753632011","pdf_url":null,"source":{"id":"https://openalex.org/S136641558","display_name":"Cloning and Stem Cells","issn_l":"1536-2302","issn":["1536-2302","1557-7457"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320443","host_organization_name":"Mary Ann Liebert, Inc.","host_organization_lineage":["https://openalex.org/P4310320443","https://openalex.org/P4310320017"],"host_organization_lineage_names":["Mary Ann Liebert, Inc.","SAGE Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cloning and Stem Cells","raw_type":"journal-article"},{"id":"pmid:12006153","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12006153","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cloning and stem cells","raw_type":"Journal Article"},{"id":"pmh:oai:digitalcommons.lsu.edu:animalsciences_pubs-1410","doi":null,"is_oa":false,"landing_page_url":"https://digitalcommons.lsu.edu/animalsciences_pubs/411","pdf_url":null,"source":{"id":"https://openalex.org/S4210169993","display_name":"Civil War Book Review","issn_l":"1528-6592","issn":["1528-6592"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310315936","host_organization_name":"Louisiana State University","host_organization_lineage":["https://openalex.org/P4310315936"],"host_organization_lineage_names":["Louisiana State University"],"type":"journal"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Faculty Publications","raw_type":"text"},{"id":"pmh:oai:repository.lsu.edu:animalsciences_pubs-1410","doi":null,"is_oa":false,"landing_page_url":"https://repository.lsu.edu/animalsciences_pubs/411","pdf_url":null,"source":{"id":"https://openalex.org/S4210169993","display_name":"Civil War Book Review","issn_l":"1528-6592","issn":["1528-6592"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310315936","host_organization_name":"Louisiana State University","host_organization_lineage":["https://openalex.org/P4310315936"],"host_organization_lineage_names":["Louisiana State University"],"type":"journal"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Faculty Publications","raw_type":"text"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.5683000087738037,"id":"https://metadata.un.org/sdg/3"},{"display_name":"Zero hunger","score":0.5666999816894531,"id":"https://metadata.un.org/sdg/2"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G3189694902","display_name":"A retrievable autologous bioengineered esophageal implant as a novel treatment for long-gap esophageal atresia","funder_award_id":"R44HD095784","funder_id":"https://openalex.org/F4320337611","funder_display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development"}],"funders":[{"id":"https://openalex.org/F4320337611","display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","ror":"https://ror.org/04byxyr05"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":42,"referenced_works":["https://openalex.org/W56867395","https://openalex.org/W167429615","https://openalex.org/W1538506308","https://openalex.org/W1549680994","https://openalex.org/W1618744733","https://openalex.org/W1826783215","https://openalex.org/W1916885428","https://openalex.org/W1921814092","https://openalex.org/W1967414895","https://openalex.org/W1967486093","https://openalex.org/W1969345130","https://openalex.org/W1970894097","https://openalex.org/W1983210873","https://openalex.org/W1988581620","https://openalex.org/W1990370275","https://openalex.org/W1992525863","https://openalex.org/W1995251730","https://openalex.org/W2007516625","https://openalex.org/W2037665991","https://openalex.org/W2045899482","https://openalex.org/W2052224645","https://openalex.org/W2052245690","https://openalex.org/W2052847242","https://openalex.org/W2056630010","https://openalex.org/W2059171126","https://openalex.org/W2070129484","https://openalex.org/W2076129653","https://openalex.org/W2088135095","https://openalex.org/W2116978641","https://openalex.org/W2117539939","https://openalex.org/W2120924438","https://openalex.org/W2133955541","https://openalex.org/W2146124982","https://openalex.org/W2149228985","https://openalex.org/W2156139452","https://openalex.org/W2342214969","https://openalex.org/W2400922434","https://openalex.org/W2407151959","https://openalex.org/W2937939790","https://openalex.org/W2992739336","https://openalex.org/W3093238716","https://openalex.org/W4237682934"],"related_works":["https://openalex.org/W2373296568","https://openalex.org/W819401820","https://openalex.org/W2096398203","https://openalex.org/W2166906871","https://openalex.org/W2364215230","https://openalex.org/W2461174694","https://openalex.org/W7098323760","https://openalex.org/W2434553591","https://openalex.org/W1715503364","https://openalex.org/W2023189903"],"abstract_inverted_index":{"Manipulation":[0],"of":[1,19,40,50,54,116],"the":[2,6,29,48,70,112,129],"pig":[3,10,71],"genome":[4,72],"has":[5,21,32],"potential":[7],"to":[8,43,102,122],"improve":[9],"production":[11],"and":[12,36,46,79],"offers":[13],"powerful":[14],"biomedical":[15],"applications.":[16],"Genetic":[17],"manipulation":[18],"mammals":[20],"been":[22],"possible":[23,64],"for":[24],"over":[25],"two":[26],"decades,":[27],"but":[28,98],"technology":[30,113],"available":[31,124],"proven":[33],"both":[34],"difficult":[35],"inefficient.":[37],"The":[38,93],"development":[39],"new":[41],"techniques":[42],"enhance":[44],"efficiency":[45],"overcome":[47],"complications":[49],"random":[51],"insertion":[52],"is":[53,95,100,114],"importance.":[55],"Nuclear":[56],"transfer":[57,87],"combined":[58],"with":[59],"homologous":[60,77],"recombination":[61],"provides":[62],"a":[63],"solution:":[65],"precise":[66],"genetic":[67],"modifications":[68],"in":[69,128],"may":[73],"be":[74,83,123],"induced":[75],"via":[76],"recombination,":[78],"viable":[80],"offspring":[81],"can":[82],"produced":[84],"by":[85],"nuclear":[86],"using":[88],"cultured":[89],"transfected":[90],"cell":[91],"lines.":[92],"technique":[94],"still":[96],"ineffective,":[97],"it":[99],"believed":[101],"have":[103],"immense":[104],"potential.":[105],"One":[106],"area":[107],"that":[108,115],"would":[109],"benefit":[110],"from":[111],"xenotransplantation:":[117],"transgenic":[118],"pigs":[119],"are":[120],"expected":[121],"as":[125],"organ":[126],"donors":[127],"foreseeable":[130],"future.":[131]},"counts_by_year":[{"year":2019,"cited_by_count":1},{"year":2015,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}