{"id":"https://openalex.org/W2172022845","doi":"https://doi.org/10.1080/00021369.1990.10870487","title":"Isolation and Characterization of Glutathione Biosynthesis-deficient Mutants inSaccharomyces cerevisiae","display_name":"Isolation and Characterization of Glutathione Biosynthesis-deficient Mutants inSaccharomyces cerevisiae","publication_year":1990,"publication_date":"1990-01-01","ids":{"openalex":"https://openalex.org/W2172022845","doi":"https://doi.org/10.1080/00021369.1990.10870487","mag":"2172022845"},"language":"en","primary_location":{"id":"doi:10.1080/00021369.1990.10870487","doi":"https://doi.org/10.1080/00021369.1990.10870487","is_oa":false,"landing_page_url":"https://doi.org/10.1080/00021369.1990.10870487","pdf_url":null,"source":{"id":"https://openalex.org/S2764346059","display_name":"Agricultural and Biological Chemistry","issn_l":"0002-1369","issn":["0002-1369","0375-8397","1881-1272","1881-1280"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Agricultural and Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://www.jstage.jst.go.jp/article/bbb1961/54/12/54_12_3145/_pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5038013342","display_name":"Yasuyuki Ohtake","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210097840","display_name":"Asahi Breweries (Japan)","ror":"https://ror.org/014aeyd41","country_code":"JP","type":"company","lineage":["https://openalex.org/I4210097840"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yasuyuki Ohtake","raw_affiliation_strings":["Central Research Laboratories, Asahi Breweries, Ltd., Omori-kita 2-13-1, Ota-ku, Tokyo 143, Japan","Central Research Laboratories, Asahi Breweries, Ltd"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Central Research Laboratories, Asahi Breweries, Ltd., Omori-kita 2-13-1, Ota-ku, Tokyo 143, Japan","institution_ids":["https://openalex.org/I4210097840"]},{"raw_affiliation_string":"Central Research Laboratories, Asahi Breweries, Ltd","institution_ids":["https://openalex.org/I4210097840"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032593119","display_name":"Atushi Satou","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210097840","display_name":"Asahi Breweries (Japan)","ror":"https://ror.org/014aeyd41","country_code":"JP","type":"company","lineage":["https://openalex.org/I4210097840"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Atushi Satou","raw_affiliation_strings":["Central Research Laboratories, Asahi Breweries, Ltd., Omori-kita 2-13-1, Ota-ku, Tokyo 143, Japan","Central Research Laboratories, Asahi Breweries, Ltd"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Central Research Laboratories, Asahi Breweries, Ltd., Omori-kita 2-13-1, Ota-ku, Tokyo 143, Japan","institution_ids":["https://openalex.org/I4210097840"]},{"raw_affiliation_string":"Central Research Laboratories, Asahi Breweries, Ltd","institution_ids":["https://openalex.org/I4210097840"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5052284101","display_name":"Seizou Yabuuchi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210097840","display_name":"Asahi Breweries (Japan)","ror":"https://ror.org/014aeyd41","country_code":"JP","type":"company","lineage":["https://openalex.org/I4210097840"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Seizou Yabuuchi","raw_affiliation_strings":["Central Research Laboratories, Asahi Breweries, Ltd., Omori-kita 2-13-1, Ota-ku, Tokyo 143, Japan","Central Research Laboratories, Asahi Breweries, Ltd"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Central Research Laboratories, Asahi Breweries, Ltd., Omori-kita 2-13-1, Ota-ku, Tokyo 143, Japan","institution_ids":["https://openalex.org/I4210097840"]},{"raw_affiliation_string":"Central Research Laboratories, Asahi Breweries, Ltd","institution_ids":["https://openalex.org/I4210097840"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I4210097840"],"apc_list":null,"apc_paid":null,"fwci":0.1956,"has_fulltext":true,"cited_by_count":34,"citation_normalized_percentile":{"value":0.4281401,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"54","issue":"12","first_page":"3145","last_page":"3150"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11481","display_name":"Sulfur Compounds in Biology","score":0.6348000168800354,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11481","display_name":"Sulfur Compounds in Biology","score":0.6348000168800354,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.20250000059604645,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11884","display_name":"Redox biology and oxidative stress","score":0.11680000275373459,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/glutathione-synthesis","display_name":"glutathione synthesis","score":0.9990000128746033},{"id":"https://openalex.org/keywords/saccharomyces-cerevisiae","display_name":"Saccharomyces cerevisiae","score":0.9390000104904175},{"id":"https://openalex.org/keywords/mutant-isolation","display_name":"mutant isolation","score":0.6520000100135803},{"id":"https://openalex.org/keywords/glutathione-synthetase-deficiency","display_name":"glutathione synthetase deficiency","score":0.8529999852180481},{"id":"https://openalex.org/keywords/glutathione-supplementation","display_name":"glutathione supplementation","score":0.8029999732971191},{"id":"https://openalex.org/keywords/complementation","display_name":"complementation","score":0.6759999990463257},{"id":"https://openalex.org/keywords/recessive-mutations","display_name":"recessive mutations","score":0.6349999904632568}],"concepts":[{"id":"https://openalex.org/C188082640","wikidata":"https://www.wikidata.org/wiki/Q1780899","display_name":"Complementation","level":4,"score":0.897932767868042},{"id":"https://openalex.org/C538909803","wikidata":"https://www.wikidata.org/wiki/Q116907","display_name":"Glutathione","level":3,"score":0.866408109664917},{"id":"https://openalex.org/C2779344277","wikidata":"https://www.wikidata.org/wiki/Q106950316","display_name":"Glutathione synthetase","level":4,"score":0.8543187975883484},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.7976930141448975},{"id":"https://openalex.org/C2777576037","wikidata":"https://www.wikidata.org/wiki/Q719725","display_name":"Saccharomyces cerevisiae","level":3,"score":0.7479033470153809},{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.7473628520965576},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.7285959720611572},{"id":"https://openalex.org/C2778592357","wikidata":"https://www.wikidata.org/wiki/Q903881","display_name":"Methylglyoxal","level":3,"score":0.6879647970199585},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5243587493896484},{"id":"https://openalex.org/C2778022156","wikidata":"https://www.wikidata.org/wiki/Q576145","display_name":"Strain (injury)","level":2,"score":0.49683740735054016},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2950732111930847},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.23438063263893127},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1708718240261078},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1080/00021369.1990.10870487","doi":"https://doi.org/10.1080/00021369.1990.10870487","is_oa":false,"landing_page_url":"https://doi.org/10.1080/00021369.1990.10870487","pdf_url":null,"source":{"id":"https://openalex.org/S2764346059","display_name":"Agricultural and Biological Chemistry","issn_l":"0002-1369","issn":["0002-1369","0375-8397","1881-1272","1881-1280"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Agricultural and Biological Chemistry","raw_type":"journal-article"},{"id":"doi:10.1271/bbb1961.54.3145","doi":"https://doi.org/10.1271/bbb1961.54.3145","is_oa":true,"landing_page_url":"https://doi.org/10.1271/bbb1961.54.3145","pdf_url":"https://www.jstage.jst.go.jp/article/bbb1961/54/12/54_12_3145/_pdf","source":{"id":"https://openalex.org/S2764346059","display_name":"Agricultural and Biological Chemistry","issn_l":"0002-1369","issn":["0002-1369","0375-8397","1881-1272","1881-1280"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Agricultural and Biological Chemistry","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.1271/bbb1961.54.3145","doi":"https://doi.org/10.1271/bbb1961.54.3145","is_oa":true,"landing_page_url":"https://doi.org/10.1271/bbb1961.54.3145","pdf_url":"https://www.jstage.jst.go.jp/article/bbb1961/54/12/54_12_3145/_pdf","source":{"id":"https://openalex.org/S2764346059","display_name":"Agricultural and Biological Chemistry","issn_l":"0002-1369","issn":["0002-1369","0375-8397","1881-1272","1881-1280"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Agricultural and Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2172022845.pdf","grobid_xml":"https://content.openalex.org/works/W2172022845.grobid-xml"},"referenced_works_count":8,"referenced_works":["https://openalex.org/W57314379","https://openalex.org/W1526043578","https://openalex.org/W1600651336","https://openalex.org/W4241916828","https://openalex.org/W4245957869","https://openalex.org/W4300130401","https://openalex.org/W6631381565","https://openalex.org/W6760068275"],"related_works":["https://openalex.org/W2016429873","https://openalex.org/W2066760016","https://openalex.org/W2081690701","https://openalex.org/W1976096476","https://openalex.org/W2166531511","https://openalex.org/W2034770144","https://openalex.org/W2042400188","https://openalex.org/W2376310287","https://openalex.org/W2030072720","https://openalex.org/W2109788296"],"abstract_inverted_index":{"Saccharomyces":[0],"cerevisiae":[1],"YNN27":[2],"(MAToe":[3],"trpl":[4],"ura3":[5],"gal2)":[6],"was":[7,52,77,133,154],"mutagenized":[8],"with":[9,86,137],"ethyl":[10],"methanesulfonate":[11],"to":[12,21,79,92,144],"isolate":[13],"mutants":[14,19,31,39],"deficient":[15,155],"in":[16,59,66,117,120,156],"glutathione":[17,29,60,112,138],"biosynthesis.After":[18],"sensitive":[20,91],"6.5":[22],"dim":[23],"methylglyoxal":[24],"were":[25,32,40,88],"screened":[26],"for,":[27],"12":[28,38],"biosynthesis-deficient":[30],"isolated.Complementation":[33],"tests":[34],"found":[35],"that":[36,49,71,148],"the":[37,73,80,103,115,127,130,145],"classified":[41],"into":[42],"two":[43],"different":[44],"classes":[45],"(YH1,":[46],"YL1),":[47],"and":[48,54,76,99,142],"each":[50],"mutant":[51,106,121],"recessive":[53],"had":[55],"a":[56],"single":[57],"mutation":[58],"biosynthesis.The":[61],"growth":[62],"of":[63,72,111,129],"both":[64],"strains":[65,122],"minimal":[67],"mediumwas":[68],"slower":[69],"than":[70,102],"parent":[74,104,131],"strain":[75,107,132,149],"restored":[78],"wild":[81],"type":[82],"level":[83],"by":[84],"supplementation":[85,136],"glutathione.They":[87],"also":[89],"more":[90],"some":[93],"chemicals":[94],"such":[95],"as":[96],"thiol-reactive":[97],"agents":[98,101],"metal-chelating":[100],"strain.The":[105],"YL1":[108],"lacked":[109],"activity":[110,128],"synthetase,":[113],"while":[114],"deficiencies":[116],"y-glutamylcysteine":[118,143,157],"synthetase":[119],"YH1and":[123],"YL1were":[124],"equivocal":[125],"because":[126],"very":[134],"weak.However,":[135],"component":[139],"amino":[140],"acids":[141],"medium":[146],"showed":[147],"YH1,":[150],"but":[151],"not":[152],"YL1,":[153],"synthetase.":[158]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":3}],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2016-06-24T00:00:00"}