{"id":"https://openalex.org/W2171810366","doi":"https://doi.org/10.1099/00221287-137-4-797","title":"Isolation and characterization of sporulation lacZ fusion mutants of Bacillus megaterium","display_name":"Isolation and characterization of sporulation lacZ fusion mutants of Bacillus megaterium","publication_year":1991,"publication_date":"1991-04-01","ids":{"openalex":"https://openalex.org/W2171810366","doi":"https://doi.org/10.1099/00221287-137-4-797","mag":"2171810366"},"language":"en","primary_location":{"id":"doi:10.1099/00221287-137-4-797","doi":"https://doi.org/10.1099/00221287-137-4-797","is_oa":false,"landing_page_url":"https://doi.org/10.1099/00221287-137-4-797","pdf_url":null,"source":{"id":"https://openalex.org/S4210238889","display_name":"Journal of General Microbiology","issn_l":"0022-1287","issn":["0022-1287","2059-9323"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310320497","host_organization_name":"Microbiology Society","host_organization_lineage":["https://openalex.org/P4310320497"],"host_organization_lineage_names":["Microbiology Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of General Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5009498403","display_name":"Yi-Ping Tao","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I102502594","display_name":"Northern Illinois University","ror":"https://ror.org/012wxa772","country_code":"US","type":"education","lineage":["https://openalex.org/I102502594"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Y.-P. Tao","raw_affiliation_strings":["Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, USA","institution_ids":["https://openalex.org/I102502594"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5026414174","display_name":"Patricia S. Vary","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I102502594","display_name":"Northern Illinois University","ror":"https://ror.org/012wxa772","country_code":"US","type":"education","lineage":["https://openalex.org/I102502594"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"P. S. Vary","raw_affiliation_strings":["Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, USA","institution_ids":["https://openalex.org/I102502594"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I102502594"],"apc_list":null,"apc_paid":null,"fwci":0.6293,"has_fulltext":false,"cited_by_count":12,"citation_normalized_percentile":{"value":0.6993189,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"137","issue":"4","first_page":"797","last_page":"806"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11515","display_name":"Bacillus and Francisella bacterial research","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bacillus-megaterium","display_name":"Bacillus megaterium","score":0.9980000257492065},{"id":"https://openalex.org/keywords/sporulation","display_name":"sporulation","score":0.7319999933242798},{"id":"https://openalex.org/keywords/genetic-mapping","display_name":"gene mapping","score":0.546999990940094},{"id":"https://openalex.org/keywords/trp-operon","display_name":"trp operon","score":0.5299999713897705},{"id":"https://openalex.org/keywords/septum-formation","display_name":"septum formation","score":0.6890000104904175},{"id":"https://openalex.org/keywords/sporulation-genes","display_name":"sporulation genes","score":0.47999998927116394},{"id":"https://openalex.org/keywords/sporulation-mutants","display_name":"sporulation mutants","score":0.4970000088214874}],"concepts":[{"id":"https://openalex.org/C2780989563","wikidata":"https://www.wikidata.org/wiki/Q148703","display_name":"Bacillus megaterium","level":3,"score":0.8980765342712402},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.842955470085144},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6903855800628662},{"id":"https://openalex.org/C170344550","wikidata":"https://www.wikidata.org/wiki/Q311359","display_name":"lac operon","level":4,"score":0.5188080668449402},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5133220553398132},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.42309021949768066},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.38204148411750793},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.3715723156929016},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.36975234746932983},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.15481573343276978}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1099/00221287-137-4-797","doi":"https://doi.org/10.1099/00221287-137-4-797","is_oa":false,"landing_page_url":"https://doi.org/10.1099/00221287-137-4-797","pdf_url":null,"source":{"id":"https://openalex.org/S4210238889","display_name":"Journal of General Microbiology","issn_l":"0022-1287","issn":["0022-1287","2059-9323"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310320497","host_organization_name":"Microbiology Society","host_organization_lineage":["https://openalex.org/P4310320497"],"host_organization_lineage_names":["Microbiology Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of General Microbiology","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":44,"referenced_works":["https://openalex.org/W119765585","https://openalex.org/W156648096","https://openalex.org/W1480366252","https://openalex.org/W1483853196","https://openalex.org/W1486358076","https://openalex.org/W1519737110","https://openalex.org/W1521290780","https://openalex.org/W1536539071","https://openalex.org/W1546783991","https://openalex.org/W1554313123","https://openalex.org/W1563454467","https://openalex.org/W1608457529","https://openalex.org/W1618458920","https://openalex.org/W1834062581","https://openalex.org/W1900856863","https://openalex.org/W1912305654","https://openalex.org/W1921058700","https://openalex.org/W1946218037","https://openalex.org/W1964807917","https://openalex.org/W1972981991","https://openalex.org/W1986038245","https://openalex.org/W1993042465","https://openalex.org/W1996823807","https://openalex.org/W2007432822","https://openalex.org/W2008044598","https://openalex.org/W2008570857","https://openalex.org/W2014509207","https://openalex.org/W2015764731","https://openalex.org/W2024041553","https://openalex.org/W2031227019","https://openalex.org/W2061243460","https://openalex.org/W2067184583","https://openalex.org/W2070974154","https://openalex.org/W2082748586","https://openalex.org/W2106550529","https://openalex.org/W2123812634","https://openalex.org/W2140680463","https://openalex.org/W2146953954","https://openalex.org/W2275967469","https://openalex.org/W2316397892","https://openalex.org/W2334573075","https://openalex.org/W2464846532","https://openalex.org/W2483932544","https://openalex.org/W4285719527"],"related_works":["https://openalex.org/W2345618973","https://openalex.org/W7220693572","https://openalex.org/W7220921001","https://openalex.org/W1605139332","https://openalex.org/W2056135799","https://openalex.org/W2037893609","https://openalex.org/W2026750051","https://openalex.org/W7008593535","https://openalex.org/W1968719162","https://openalex.org/W2156056369"],"abstract_inverted_index":{"Summary:":[0],"A":[1],"derivative":[2],"of":[3,9,47,62,106,111,113,131,139,149,193],"Bacillus":[4],"megaterium":[5,198],"QM":[6],"B1551":[7],"cured":[8],"all":[10,72],"seven":[11],"resident":[12],"plasmids":[13],"was":[14,24,157,164],"mutated":[15],"to":[16,27,53,125],"Lac\u20132.":[17],"Transposon":[18],"Tn917":[19],"carrying":[20],"lacZ":[21],"and":[22,57,71,100,136,199],"cat":[23],"then":[25],"used":[26,208],"isolate":[28],"four":[29,73,85],"spo:":[30],":lacZ-cat":[31],"fusion":[32,40,204],"mutants":[33,49,64,86,115,195],"by":[34,78],"screening":[35],"for":[36],"colonies":[37],"expressing":[38,116],"the":[39,48,63,68,81,104,114,117,129,143,160,172,189],"in":[41,80,91,134,142,159,175,196,209],"stationary":[42],"phase.":[43],"The":[44],"sporulation":[45,69,92,194],"frequencies":[46],"ranged":[50],"from":[51],"10\u20137":[52],"10\u20132.":[54],"Macrolide,":[55],"lincosamide":[56],"streptogramin":[58],"B":[59],"resistance":[60],"(MLSr)":[61],"cotransduced":[65],"100%":[66],"with":[67],"defect":[70,123],"mutations":[74,173],"mapped":[75],"near":[76],"trp":[77],"transduction":[79],"order:":[82],"trp\u2013hisH\u2013spo.":[83],"All":[84],"isolated":[87],"were":[88,174],"defective":[89],"early":[90,179],"since":[93],"\u03b2-galactosidase":[94],"could":[95],"be":[96,207],"detected":[97,158],"between":[98],"zero":[99],"2":[101],"h":[102],"after":[103,137],"end":[105],"exponential":[107],"growth.":[108],"Electron":[109],"microscopy":[110],"two":[112,178],"enzyme":[118],"at":[119,128,176],"t1\u2013t2":[120],"revealed":[121],"a":[122],"prior":[124],"or":[126,146,154],"just":[127],"beginning":[130],"septum":[132,140],"formation":[133,141],"one,":[135],"completion":[138],"other.":[144],"Little":[145],"no":[147],"synthesis":[148],"dipicolinic":[150],"acid,":[151],"glucose":[152,183],"dehydrogenase":[153,184],"alkaline":[155],"phosphatase":[156],"mutants,":[161],"but":[162],"each":[163],"neutral":[165],"protease":[166],"positive.":[167],"These":[168],"results":[169],"show":[170],"that":[171,202],"least":[177],"genes":[180],"expressed":[181],"before":[182],"production.":[185],"This":[186],"study":[187],"represents":[188],"first":[190],"genetic":[191],"characterization":[192],"B.":[197],"also":[200],"demonstrates":[201],"gene":[203],"technology":[205],"can":[206],"this":[210],"species.":[211]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2014,"cited_by_count":2}],"updated_date":"2025-11-06T03:46:38.306776","created_date":"2016-06-24T00:00:00"}