{"id":"https://openalex.org/W2171486966","doi":"https://doi.org/10.1111/j.1365-313x.2005.02636.x","title":"A high\u2010throughput screen for genes from castor that boost hydroxy fatty acid accumulation in seed oils of transgenic Arabidopsis","display_name":"A high\u2010throughput screen for genes from castor that boost hydroxy fatty acid accumulation in seed oils of transgenic Arabidopsis","publication_year":2006,"publication_date":"2006-01-27","ids":{"openalex":"https://openalex.org/W2171486966","doi":"https://doi.org/10.1111/j.1365-313x.2005.02636.x","mag":"2171486966","pmid":"https://pubmed.ncbi.nlm.nih.gov/16460516"},"language":"en","primary_location":{"id":"doi:10.1111/j.1365-313x.2005.02636.x","doi":"https://doi.org/10.1111/j.1365-313x.2005.02636.x","is_oa":true,"landing_page_url":"https://doi.org/10.1111/j.1365-313x.2005.02636.x","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1365-313X.2005.02636.x","source":{"id":"https://openalex.org/S67862205","display_name":"The Plant Journal","issn_l":"0960-7412","issn":["0960-7412","1365-313X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Plant Journal","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1365-313X.2005.02636.x","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100432051","display_name":"Chaofu Lu","orcid":"https://orcid.org/0000-0001-6901-7490","observed_orcids":["https://orcid.org/0000-0001-6901-7490"]},"institutions":[{"id":"https://openalex.org/I23732399","display_name":"Montana State University","ror":"https://ror.org/02w0trx84","country_code":"US","type":"education","lineage":["https://openalex.org/I23732399","https://openalex.org/I4210126032"]},{"id":"https://openalex.org/I72951846","display_name":"Washington State University","ror":"https://ror.org/05dk0ce17","country_code":"US","type":"education","lineage":["https://openalex.org/I72951846"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Chaofu Lu","raw_affiliation_strings":["Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA","Present address: Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, MT 59717, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA","institution_ids":["https://openalex.org/I72951846"]},{"raw_affiliation_string":"Present address: Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, MT 59717, USA","institution_ids":["https://openalex.org/I23732399"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020018880","display_name":"Martin S. Fulda","orcid":"https://orcid.org/0000-0001-5734-067X","observed_orcids":["https://orcid.org/0000-0001-5734-067X"]},"institutions":[{"id":"https://openalex.org/I74656192","display_name":"University of G\u00f6ttingen","ror":"https://ror.org/01y9bpm73","country_code":"DE","type":"education","lineage":["https://openalex.org/I74656192"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Martin Fulda","raw_affiliation_strings":["Present address: Department of Plant Biochemistry, Georg-August University, Goettingen, Justus-von-Liebig Weg 11, D37077 Goettingen, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Present address: Department of Plant Biochemistry, Georg-August University, Goettingen, Justus-von-Liebig Weg 11, D37077 Goettingen, Germany","institution_ids":["https://openalex.org/I74656192"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5052221508","display_name":"James G. Wallis","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I72951846","display_name":"Washington State University","ror":"https://ror.org/05dk0ce17","country_code":"US","type":"education","lineage":["https://openalex.org/I72951846"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"James G. Wallis","raw_affiliation_strings":["Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA","institution_ids":["https://openalex.org/I72951846"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5078539252","display_name":"John Browse","orcid":"https://orcid.org/0000-0002-2554-2821","observed_orcids":["https://orcid.org/0000-0002-2554-2821"]},"institutions":[{"id":"https://openalex.org/I23732399","display_name":"Montana State University","ror":"https://ror.org/02w0trx84","country_code":"US","type":"education","lineage":["https://openalex.org/I23732399","https://openalex.org/I4210126032"]},{"id":"https://openalex.org/I72951846","display_name":"Washington State University","ror":"https://ror.org/05dk0ce17","country_code":"US","type":"education","lineage":["https://openalex.org/I72951846"]},{"id":"https://openalex.org/I74656192","display_name":"University of G\u00f6ttingen","ror":"https://ror.org/01y9bpm73","country_code":"DE","type":"education","lineage":["https://openalex.org/I74656192"]}],"countries":["DE","US"],"is_corresponding":true,"raw_author_name":"John Browse","raw_affiliation_strings":["Department of Plant Biochemistry, Georg-August University, Goettingen, Justus-von-Liebig Weg 11, D37077 Goettingen, Germany","Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, MT 59717, USA","Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Plant Biochemistry, Georg-August University, Goettingen, Justus-von-Liebig Weg 11, D37077 Goettingen, Germany","institution_ids":["https://openalex.org/I74656192"]},{"raw_affiliation_string":"Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, MT 59717, USA","institution_ids":["https://openalex.org/I23732399"]},{"raw_affiliation_string":"Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA","institution_ids":["https://openalex.org/I72951846"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5078539252"],"corresponding_institution_ids":["https://openalex.org/I23732399","https://openalex.org/I72951846","https://openalex.org/I74656192"],"apc_list":{"value":5450,"currency":"USD","value_usd":5450},"apc_paid":null,"fwci":3.1733,"has_fulltext":true,"cited_by_count":148,"citation_normalized_percentile":{"value":0.91863385,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":95,"max":99},"biblio":{"volume":"45","issue":"5","first_page":"847","last_page":"856"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11844","display_name":"Lipid metabolism and biosynthesis","score":0.9717000126838684,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11844","display_name":"Lipid metabolism and biosynthesis","score":0.9717000126838684,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":0.015300000086426735,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.004100000020116568,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hydroxy-fatty-acids","display_name":"hydroxy fatty acids","score":0.8849999904632568},{"id":"https://openalex.org/keywords/transgenic-arabidopsis","display_name":"transgenic Arabidopsis","score":0.828000009059906},{"id":"https://openalex.org/keywords/high-throughput-screening","display_name":"high-throughput screening","score":0.7310000061988831},{"id":"https://openalex.org/keywords/fatty-acid-synthesis","display_name":"fatty acid synthesis","score":0.6159999966621399},{"id":"https://openalex.org/keywords/functional-genomics","display_name":"functional genomics","score":0.546999990940094}],"concepts":[{"id":"https://openalex.org/C2779491563","wikidata":"https://www.wikidata.org/wiki/Q157892","display_name":"Arabidopsis","level":4,"score":0.8543174862861633},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.624979555606842},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.6242792010307312},{"id":"https://openalex.org/C2775988993","wikidata":"https://www.wikidata.org/wiki/Q158695","display_name":"Arabidopsis thaliana","level":4,"score":0.5801286101341248},{"id":"https://openalex.org/C122172344","wikidata":"https://www.wikidata.org/wiki/Q1503477","display_name":"Genetically modified crops","level":4,"score":0.5695616006851196},{"id":"https://openalex.org/C543025807","wikidata":"https://www.wikidata.org/wiki/Q61476","display_name":"Fatty acid","level":2,"score":0.5382601618766785},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4984169006347656},{"id":"https://openalex.org/C113442657","wikidata":"https://www.wikidata.org/wiki/Q25624","display_name":"Endosperm","level":2,"score":0.4919273853302002},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.4494209587574005},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4306168258190155},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.0852942168712616}],"mesh":[{"descriptor_ui":"D002367","descriptor_name":"Ricinus communis","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D002849","descriptor_name":"Chromatography, Gas","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D005227","descriptor_name":"Fatty Acids","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012639","descriptor_name":"Seeds","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D014170","descriptor_name":"Transformation, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015336","descriptor_name":"Molecular Probe Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015723","descriptor_name":"Gene Library","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017343","descriptor_name":"Genes, Plant","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D019470","descriptor_name":"Biolistics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D030821","descriptor_name":"Plants, Genetically Modified","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1111/j.1365-313x.2005.02636.x","doi":"https://doi.org/10.1111/j.1365-313x.2005.02636.x","is_oa":true,"landing_page_url":"https://doi.org/10.1111/j.1365-313x.2005.02636.x","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1365-313X.2005.02636.x","source":{"id":"https://openalex.org/S67862205","display_name":"The Plant Journal","issn_l":"0960-7412","issn":["0960-7412","1365-313X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Plant Journal","raw_type":"journal-article"},{"id":"pmid:16460516","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16460516","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Plant journal : for cell and molecular biology","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1111/j.1365-313x.2005.02636.x","doi":"https://doi.org/10.1111/j.1365-313x.2005.02636.x","is_oa":true,"landing_page_url":"https://doi.org/10.1111/j.1365-313x.2005.02636.x","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1365-313X.2005.02636.x","source":{"id":"https://openalex.org/S67862205","display_name":"The Plant Journal","issn_l":"0960-7412","issn":["0960-7412","1365-313X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Plant Journal","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger","score":0.875}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320306114","display_name":"U.S. Department of Agriculture","ror":"https://ror.org/01na82s61"},{"id":"https://openalex.org/F4320309913","display_name":"Washington State University","ror":"https://ror.org/05dk0ce17"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2171486966.pdf","grobid_xml":"https://content.openalex.org/works/W2171486966.grobid-xml"},"referenced_works_count":31,"referenced_works":["https://openalex.org/W81770158","https://openalex.org/W113855239","https://openalex.org/W204037375","https://openalex.org/W1486942224","https://openalex.org/W1501973340","https://openalex.org/W1854051914","https://openalex.org/W1947410838","https://openalex.org/W1969752714","https://openalex.org/W1981614977","https://openalex.org/W1983680558","https://openalex.org/W1995838394","https://openalex.org/W1995945511","https://openalex.org/W1996594369","https://openalex.org/W1998746328","https://openalex.org/W2016650856","https://openalex.org/W2022318444","https://openalex.org/W2031171163","https://openalex.org/W2032229319","https://openalex.org/W2032656815","https://openalex.org/W2036648920","https://openalex.org/W2043796764","https://openalex.org/W2050548146","https://openalex.org/W2058753095","https://openalex.org/W2061409430","https://openalex.org/W2074122548","https://openalex.org/W2096375668","https://openalex.org/W2098933963","https://openalex.org/W2106629711","https://openalex.org/W2116122104","https://openalex.org/W2155530972","https://openalex.org/W2174731688"],"related_works":["https://openalex.org/W2428673022","https://openalex.org/W4200255583","https://openalex.org/W4283077092","https://openalex.org/W1968078797","https://openalex.org/W2063140502","https://openalex.org/W2016650856","https://openalex.org/W2907429654","https://openalex.org/W7218709784","https://openalex.org/W2150397188","https://openalex.org/W2084146281"],"abstract_inverted_index":{"It":[0],"is":[1,193],"desirable":[2],"to":[3,16,85,178,195],"produce":[4],"high":[5,65],"homogeneity":[6],"of":[7,21,27,32,41,50,110,114,153,208],"novel":[8],"fatty":[9,34,43,52,81,92,155,180],"acids":[10,35,44,53,156],"in":[11,38,45,54,67,95,105,157,183,205],"oilseeds":[12],"through":[13],"genetic":[14],"engineering":[15],"meet":[17],"the":[18,22,72,111,170,198],"increasing":[19],"demands":[20],"oleo-chemical":[23],"industry.":[24],"However,":[25],"expression":[26],"key":[28],"enzymes":[29],"for":[30,64],"biosynthesis":[31],"industrial":[33],"usually":[36],"results":[37],"low":[39],"levels":[40],"desired":[42],"transgenic":[46,68,96,133,158,184],"oilseeds.":[47],"The":[48,131],"abundance":[49],"derivatized":[51],"their":[55,175],"natural":[56],"species":[57],"suggests":[58],"that":[59,88,149,192],"additional":[60],"genes":[61,87],"are":[62],"needed":[63],"production":[66],"plants.":[69],"We":[70,141],"used":[71],"model":[73],"oilseed":[74,196],"plant":[75,209],"Arabidopsis":[76,129,185],"thaliana":[77],"expressing":[78],"a":[79,101,122],"castor":[80,116,147],"acid":[82,93,181],"hydroxylase":[83],"(FAH12)":[84],"identify":[86],"can":[89,107,202],"boost":[90],"hydroxy":[91,154,179],"accumulation":[94,182],"seeds.":[97,186],"Here":[98],"we":[99,188,200],"describe":[100,189],"high-throughput":[102,138],"approach":[103,191],"that,":[104],"principle,":[106],"allow":[108],"testing":[109],"entire":[112],"transcriptome":[113],"developing":[115],"seed":[117],"endosperm":[118],"by":[119,137,165],"shotgun":[120],"transforming":[121],"full-length":[123],"cDNA":[124],"library":[125],"into":[126,169],"an":[127,190],"FAH12-expressing":[128,171],"line.":[130],"resulting":[132],"seeds":[134],"were":[135,162],"screened":[136],"gas":[139],"chromatography.":[140],"obtained":[142],"several":[143],"lines":[144],"transformed":[145],"with":[146],"cDNAs":[148,161],"contained":[150],"increased":[151],"amounts":[152],"Arabidopsis.":[159],"These":[160],"then":[163],"isolated":[164],"PCR":[166],"and":[167,211],"retransformed":[168],"line,":[172],"thus":[173],"confirming":[174],"beneficial":[176],"contributions":[177],"Although":[187],"targeted":[194],"engineering,":[197],"methods":[199],"developed":[201],"be":[203],"applied":[204],"many":[206],"areas":[207],"biotechnology":[210],"functional":[212],"genomic":[213],"research.":[214]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":7},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":9},{"year":2021,"cited_by_count":8},{"year":2020,"cited_by_count":15},{"year":2019,"cited_by_count":10},{"year":2018,"cited_by_count":8},{"year":2017,"cited_by_count":7},{"year":2016,"cited_by_count":11},{"year":2015,"cited_by_count":9},{"year":2014,"cited_by_count":4},{"year":2013,"cited_by_count":7},{"year":2012,"cited_by_count":9}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}