{"id":"https://openalex.org/W2170273540","doi":null,"title":"Investigation of antiviral and anticancer nucleoside analog substrate recognition of drosophila melanogaster and herpes virus deoxyribonucleoside kinases","display_name":"Investigation of antiviral and anticancer nucleoside analog substrate recognition of drosophila melanogaster and herpes virus deoxyribonucleoside kinases","publication_year":2006,"publication_date":"2006-09-15","ids":{"openalex":"https://openalex.org/W2170273540","mag":"2170273540"},"language":"en","primary_location":{"id":"pmh:oai:figshare.com:article/26904229","doi":null,"is_oa":false,"landing_page_url":"https://figshare.com/articles/thesis/Investigation_of_antiviral_and_anticancer_nucleoside_analog_substrate_recognition_of_drosophila_melanogaster_and_herpes_virus_deoxyribonucleoside_kinases/26904229","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://hdl.handle.net/10616/39256","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5044603408","display_name":"Nicola Solaroli","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Nicola Solaroli","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5044603408"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.8779000043869019,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.8779000043869019,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10625","display_name":"Herpesvirus Infections and Treatments","score":0.02319999970495701,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10526","display_name":"HIV/AIDS drug development and treatment","score":0.008200000040233135,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/antiviral-nucleoside-analogues","display_name":"antiviral nucleosides","score":0.871999979019165},{"id":"https://openalex.org/keywords/suicide-gene-therapy","display_name":"suicide gene therapy","score":0.9459999799728394},{"id":"https://openalex.org/keywords/herpes-simplex-virus-type-1-thymidine-kinase","display_name":"herpes simplex virus type 1 thymidine kinase","score":0.8529999852180481},{"id":"https://openalex.org/keywords/ganciclovir","display_name":"ganciclovir","score":0.8420000076293945},{"id":"https://openalex.org/keywords/pyrimidine-nucleosides","display_name":"pyrimidine nucleosides","score":0.7300000190734863},{"id":"https://openalex.org/keywords/purine-nucleosides","display_name":"purine nucleosides","score":0.9660000205039978},{"id":"https://openalex.org/keywords/drosophila-melanogaster","display_name":"Drosophila melanogaster","score":0.7419999837875366},{"id":"https://openalex.org/keywords/deoxycytidine-kinase","display_name":"deoxycytidine kinase","score":0.6809999942779541},{"id":"https://openalex.org/keywords/deoxyguanosine-kinase","display_name":"deoxyguanosine kinase","score":0.9390000104904175}],"concepts":[{"id":"https://openalex.org/C2776123222","wikidata":"https://www.wikidata.org/wiki/Q28751","display_name":"Deoxyribonucleoside","level":3,"score":0.8103119134902954},{"id":"https://openalex.org/C2777333352","wikidata":"https://www.wikidata.org/wiki/Q2689559","display_name":"Nucleoside analogue","level":3,"score":0.6369055509567261},{"id":"https://openalex.org/C184235292","wikidata":"https://www.wikidata.org/wiki/Q421851","display_name":"Kinase","level":2,"score":0.5782758593559265},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.5576954483985901},{"id":"https://openalex.org/C2776543447","wikidata":"https://www.wikidata.org/wiki/Q28734","display_name":"Nucleoside","level":2,"score":0.5478371381759644},{"id":"https://openalex.org/C2780104201","wikidata":"https://www.wikidata.org/wiki/Q130888","display_name":"Drosophila melanogaster","level":3,"score":0.4802687168121338},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3769986927509308},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3351480960845947},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.20390576124191284},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.15285679697990417},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.08934447169303894}],"mesh":[],"locations_count":3,"locations":[{"id":"pmh:oai:figshare.com:article/26904229","doi":null,"is_oa":false,"landing_page_url":"https://figshare.com/articles/thesis/Investigation_of_antiviral_and_anticancer_nucleoside_analog_substrate_recognition_of_drosophila_melanogaster_and_herpes_virus_deoxyribonucleoside_kinases/26904229","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},{"id":"pmh:oai:openarchive.ki.se:10616/39256","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10616/39256","pdf_url":null,"source":{"id":"https://openalex.org/S4306400147","display_name":"Open Archive (Karolinska Institutet)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I28166907","host_organization_name":"Karolinska Institutet","host_organization_lineage":["https://openalex.org/I28166907"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/doctoralThesis"},{"id":"mag:2170273540","doi":null,"is_oa":false,"landing_page_url":"https://openarchive.ki.se/xmlui/bitstream/10616/39256/1/thesis.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:openarchive.ki.se:10616/39256","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10616/39256","pdf_url":null,"source":{"id":"https://openalex.org/S4306400147","display_name":"Open Archive (Karolinska Institutet)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I28166907","host_organization_name":"Karolinska Institutet","host_organization_lineage":["https://openalex.org/I28166907"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/doctoralThesis"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.504800021648407,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":245,"referenced_works":["https://openalex.org/W51502992","https://openalex.org/W85824607","https://openalex.org/W89810895","https://openalex.org/W94096219","https://openalex.org/W115689189","https://openalex.org/W133846736","https://openalex.org/W195270458","https://openalex.org/W649428027","https://openalex.org/W1482773835","https://openalex.org/W1500000190","https://openalex.org/W1502628406","https://openalex.org/W1505281346","https://openalex.org/W1507543762","https://openalex.org/W1508258186","https://openalex.org/W1509464307","https://openalex.org/W1511106986","https://openalex.org/W1527136114","https://openalex.org/W1527742565","https://openalex.org/W1529552889","https://openalex.org/W1532195648","https://openalex.org/W1533006219","https://openalex.org/W1536062643","https://openalex.org/W1542131798","https://openalex.org/W1543819126","https://openalex.org/W1544410213","https://openalex.org/W1548955637","https://openalex.org/W1557333345","https://openalex.org/W1572756764","https://openalex.org/W1576093526","https://openalex.org/W1580143110","https://openalex.org/W1587133637","https://openalex.org/W1591573176","https://openalex.org/W1598500461","https://openalex.org/W1602693770","https://openalex.org/W1603423304","https://openalex.org/W1604895913","https://openalex.org/W1607665096","https://openalex.org/W1637024185","https://openalex.org/W1665642577","https://openalex.org/W1680634601","https://openalex.org/W1723950406","https://openalex.org/W1740448688","https://openalex.org/W1785960949","https://openalex.org/W1822105754","https://openalex.org/W1825196614","https://openalex.org/W1839702653","https://openalex.org/W1846390947","https://openalex.org/W1859008192","https://openalex.org/W1876180597","https://openalex.org/W1932680472","https://openalex.org/W1939536163","https://openalex.org/W1950306259","https://openalex.org/W1963729009","https://openalex.org/W1963755435","https://openalex.org/W1966663368","https://openalex.org/W1967378577","https://openalex.org/W1967830103","https://openalex.org/W1968050545","https://openalex.org/W1968982209","https://openalex.org/W1969072663","https://openalex.org/W1969125109","https://openalex.org/W1969827926","https://openalex.org/W1970227790","https://openalex.org/W1970343207","https://openalex.org/W1970745601","https://openalex.org/W1971962180","https://openalex.org/W1972744319","https://openalex.org/W1973504318","https://openalex.org/W1973780092","https://openalex.org/W1975598561","https://openalex.org/W1975804438","https://openalex.org/W1976379944","https://openalex.org/W1977250549","https://openalex.org/W1978070317","https://openalex.org/W1978155015","https://openalex.org/W1978902758","https://openalex.org/W1978969849","https://openalex.org/W1981619501","https://openalex.org/W1981859457","https://openalex.org/W1984105748","https://openalex.org/W1984430631","https://openalex.org/W1985065592","https://openalex.org/W1985309622","https://openalex.org/W1985744441","https://openalex.org/W1986270813","https://openalex.org/W1988089055","https://openalex.org/W1989994943","https://openalex.org/W1992322591","https://openalex.org/W1993893510","https://openalex.org/W1994689670","https://openalex.org/W1994701391","https://openalex.org/W1996052541","https://openalex.org/W1996498777","https://openalex.org/W1998558868","https://openalex.org/W1999831215","https://openalex.org/W2000382060","https://openalex.org/W2000564682","https://openalex.org/W2002240793","https://openalex.org/W2003370236","https://openalex.org/W2003634190","https://openalex.org/W2003684793","https://openalex.org/W2003787543","https://openalex.org/W2005278252","https://openalex.org/W2005730881","https://openalex.org/W2005804421","https://openalex.org/W2006821928","https://openalex.org/W2008924348","https://openalex.org/W2010236625","https://openalex.org/W2010462123","https://openalex.org/W2010534631","https://openalex.org/W2012013941","https://openalex.org/W2012366500","https://openalex.org/W2015321999","https://openalex.org/W2015848315","https://openalex.org/W2016044792","https://openalex.org/W2016628806","https://openalex.org/W2017072113","https://openalex.org/W2017505748","https://openalex.org/W2020283319","https://openalex.org/W2020738190","https://openalex.org/W2022237067","https://openalex.org/W2022474701","https://openalex.org/W2027719195","https://openalex.org/W2027797081","https://openalex.org/W2028027213","https://openalex.org/W2028547310","https://openalex.org/W2029833235","https://openalex.org/W2029931619","https://openalex.org/W2030213100","https://openalex.org/W2031122882","https://openalex.org/W2031168538","https://openalex.org/W2033863142","https://openalex.org/W2035309129","https://openalex.org/W2035663444","https://openalex.org/W2037356686","https://openalex.org/W2040175583","https://openalex.org/W2041421057","https://openalex.org/W2041743557","https://openalex.org/W2043986176","https://openalex.org/W2046370286","https://openalex.org/W2046749013","https://openalex.org/W2048967376","https://openalex.org/W2050113079","https://openalex.org/W2050200363","https://openalex.org/W2051090621","https://openalex.org/W2051979006","https://openalex.org/W2054635243","https://openalex.org/W2055173188","https://openalex.org/W2058937296","https://openalex.org/W2059868174","https://openalex.org/W2060174297","https://openalex.org/W2063029761","https://openalex.org/W2065543158","https://openalex.org/W2066170631","https://openalex.org/W2066552121","https://openalex.org/W2066707855","https://openalex.org/W2066939106","https://openalex.org/W2068261013","https://openalex.org/W2069094439","https://openalex.org/W2069946765","https://openalex.org/W2072267298","https://openalex.org/W2073478593","https://openalex.org/W2073853724","https://openalex.org/W2074051138","https://openalex.org/W2074868392","https://openalex.org/W2075225888","https://openalex.org/W2076316345","https://openalex.org/W2076615266","https://openalex.org/W2077839707","https://openalex.org/W2079030916","https://openalex.org/W2079041341","https://openalex.org/W2079161571","https://openalex.org/W2079723980","https://openalex.org/W2079805238","https://openalex.org/W2080942477","https://openalex.org/W2082703788","https://openalex.org/W2084876684","https://openalex.org/W2087846171","https://openalex.org/W2088071806","https://openalex.org/W2089185166","https://openalex.org/W2090209699","https://openalex.org/W2090495875","https://openalex.org/W2090518535","https://openalex.org/W2092167912","https://openalex.org/W2094477218","https://openalex.org/W2095109478","https://openalex.org/W2099768013","https://openalex.org/W2103961908","https://openalex.org/W2110232411","https://openalex.org/W2110392022","https://openalex.org/W2112844851","https://openalex.org/W2114002124","https://openalex.org/W2114976949","https://openalex.org/W2115567167","https://openalex.org/W2118545186","https://openalex.org/W2118883966","https://openalex.org/W2120034947","https://openalex.org/W2120679827","https://openalex.org/W2124570744","https://openalex.org/W2125515637","https://openalex.org/W2130575875","https://openalex.org/W2137693314","https://openalex.org/W2138009128","https://openalex.org/W2140096403","https://openalex.org/W2141652419","https://openalex.org/W2142237411","https://openalex.org/W2142957146","https://openalex.org/W2147708332","https://openalex.org/W2147891085","https://openalex.org/W2148769388","https://openalex.org/W2150472397","https://openalex.org/W2152321288","https://openalex.org/W2159818060","https://openalex.org/W2162288115","https://openalex.org/W2163186208","https://openalex.org/W2163215727","https://openalex.org/W2163221081","https://openalex.org/W2164051062","https://openalex.org/W2165012318","https://openalex.org/W2169012376","https://openalex.org/W2169058303","https://openalex.org/W2172724751","https://openalex.org/W2287967910","https://openalex.org/W2318638346","https://openalex.org/W2320848821","https://openalex.org/W2321167123","https://openalex.org/W2326476344","https://openalex.org/W2327419156","https://openalex.org/W2342812949","https://openalex.org/W2344610797","https://openalex.org/W2345402138","https://openalex.org/W2397191166","https://openalex.org/W2404877004","https://openalex.org/W2407301167","https://openalex.org/W2409865406","https://openalex.org/W2411838709","https://openalex.org/W2415772247","https://openalex.org/W2416263105","https://openalex.org/W2418607588","https://openalex.org/W2419364246","https://openalex.org/W2467272588","https://openalex.org/W2616200793","https://openalex.org/W2741083311","https://openalex.org/W2922395101","https://openalex.org/W2993767461"],"related_works":["https://openalex.org/W1998558868","https://openalex.org/W2174712550","https://openalex.org/W2002240793","https://openalex.org/W7000940438","https://openalex.org/W2029837453","https://openalex.org/W2077940366","https://openalex.org/W2002870743","https://openalex.org/W2108092746","https://openalex.org/W2072267298","https://openalex.org/W2079723980"],"abstract_inverted_index":{"The":[0,60,125,147,180,201,249,310,505,666],"deoxyribonucleoside":[1,64,709,809],"kinase":[2,37,40,44,57,130,150,747,786,810],"of":[3,123,157,164,184,204,220,225,253,297,303,313,323,365,380,384,433,560,575,614,639,662,687,705,733,740,776,804],"the":[4,34,51,73,134,142,154,158,223,241,262,329,341,366,378,381,385,431,441,455,466,474,484,500,508,513,535,548,558,561,566,576,628,663,677,805],"fruit":[5],"fly":[6],"Drosophila":[7,706,783,806],"melanogaster":[8,707,784,807],"(Dm-dNK)":[9],"is":[10,30,133,151,259],"a":[11,207,244,290,320,434,543,553,622,634,636,734,749],"multisubstrate":[12,708,808],"enzyme":[13,242,263,386,511,577],"that":[14,218,407,430,565,573,611],"phosphorylates":[15,235],"pyrimidine":[16,239,247,256,268,753],"and":[17,25,42,62,100,162,186,213,238,255,270,316,326,352,372,387,425,459,464,490,498,572,602,632,660,669],"purine":[18,237,254,278,518,755,777],"deoxyribonucleosides":[19,80,421],"as":[20,22,47,49,117,206,594,633],"well":[21,48],"several":[23,229],"anticancer":[24],"antiviral":[26],"nucleoside":[27,99,101,107,129,144,149,160,172,231,257,271,279,305,503,519,537,591,756,778,785],"analogs.":[28,232,272,504],"Dm-dNK":[29,71,92,185,205,221,234,351,390,408,544,568],"sequence":[31],"related":[32],"to":[33,50,75,87,105,228,288,376,388,423,439,446,476,502,517,523,528,547,557,589,617,674,754],"human":[35,61],"deoxycytidine":[36],"(dCK),":[38],"deoxyguanosine":[39],"(dGK)":[41],"thymidine":[43,56,746,812],"2":[45],"(M),":[46],"herpes":[52,741],"simplex":[53,742],"virus":[54,743],"type-1":[55],"(Hsv-1":[58],"TK).":[59],"viral":[63],"kinases":[65,110],"can":[66],"phosphorylate":[67,76,477],"multiple":[68],"deoxyribonucleosides,":[69],"whereas":[70],"has":[72,210,215,243,403,415],"ability":[74,475],"all":[77],"naturally":[78],"occurring":[79],"required":[81],"for":[82,98,114,171,197,246,267,396,420],"DNA":[83],"replication.":[84],"In":[85,652],"addition":[86],"its":[88,187],"broad":[89,181],"substrate":[90,182,335,342,382,442],"specificity,":[91],"also":[93,345,404,428,541,609],"exhibits":[94,264,346],"higher":[95,265,291,417,623,637],"catalytic":[96,189,251,418],"rates":[97,419],"analog":[102,145,173],"phosphorylation":[103,174,258,684],"compared":[104,422,527,616],"other":[106],"kinases.":[108],"Nucleoside":[109],"are":[111,175,337],"being":[112],"investigated":[113],"possible":[115,202],"use":[116,203],"suicide":[118,131,148,178,198,208,274,399],"genes":[119],"in":[120,139,153,327,334,374,398,492,578,585,621,627,780],"combined":[121],"gene/chemotherapy":[122],"cancer.":[124],"most":[126,485],"commonly":[127],"studied":[128],"gene":[132,137,196,199,209,275,400],"HSV-1":[135,165,317,353,650,664],"TK":[136,166,495],"used":[138],"combination":[140],"with":[141,167,392,409,463,521],"guanosine":[143],"ganciclovir.":[146],"ratelimiting":[152],"pharmacological":[155],"activation":[156],"cytotoxic":[159,230],"analogs,":[161],"mutants":[163,391,704],"improved":[168,393],"biochemical":[169],"properties":[170,395],"more":[176],"efficient":[177,486],"genes.":[179],"specificity":[183,383,443],"high":[188],"rate":[190,252,638],"makes":[191],"it":[192,214,426],"an":[193,493,579,586],"interesting":[194],"candidate":[195],"therapy.":[200,401],"been":[211,216,405],"evaluated":[212],"shown":[217,406],"over-expression":[219],"enhances":[222],"sensitivity":[224,501,516,588],"cancer":[226],"cells":[227,300,506,531,533,781],"Although":[233],"both":[236],"nucleosides,":[240],"preference":[245],"nucleosides.":[248],"maximal":[250],"similar,":[260],"but":[261],"affinity":[266],"nucleosides":[269],"For":[273],"therapy":[276],"application,":[277],"analogs":[280,306,520,592,779],"may":[281],"be":[282],"preferred":[283],"because":[284],"these":[285,324,450],"compounds":[286],"appear":[287],"induce":[289],"bystander":[292],"cell":[293,496,582],"killing,":[294],"i.e.":[295],"killing":[296],"untransduced":[298,529],"neighboring":[299],"by":[301,551],"transfer":[302],"phosphorylated":[304],"via":[307],"gap":[308],"junctions.":[309],"solved":[311],"structures":[312],"Dm-dNK,":[314,615],"dGK":[315],"TK,":[318],"reveal":[319],"common":[321],"folding":[322],"enzymes":[325,458,487,671],"particular":[328,653],"amino":[330,411,436,468,737],"acid":[331,412,469,738],"residues":[332,367],"involved":[333],"interactions":[336],"highly":[338],"conserved.":[339],"However,":[340],"binding":[343],"site":[344,362,703],"some":[347,590],"major":[348],"differences":[349],"between":[350],"TK.":[354,651,665],"Based":[355,448],"on":[356,449,649,658],"this":[357],"structural":[358,647],"information":[359],"we":[360,453,654],"performed":[361,655],"directed":[363],"mutagenesis":[364,432,657],"Asn28,":[368],"I1e29,":[369],"Phe1":[370],"14":[371],"G1n81":[373],"order":[375],"understand":[377],"determinants":[379],"find":[389],"kinetic":[394],"application":[397],"It":[402],"20":[410,467],"C-terminal":[413,470],"deletion":[414],"even":[416],"wild-type,":[424],"was":[427,569],"reported":[429,456],"few":[435],"acids":[437],"allows":[438,748],"change":[440],"from":[444,752],"pyrimidines":[445],"purines.":[447],"previous":[451],"studies":[452,606,648],"constructed":[454],"mutated":[457,667],"designed":[460],"new":[461],"mutations,":[462],"without":[465],"deletion.":[471],"We":[472,480,539,563,645],"measured":[473],"ganciclovir":[478],"(GCV).":[479],"have":[481,540,675],"finally":[482],"selected":[483],"phosphorylating":[488],"GCV":[489,683],"expressed":[491],"osteosarcoma":[494,580],"line":[497,583],"determined":[499],"expressing":[507,534,782],"Met88Arg":[509],"mutant":[510],"showed":[512,564,610],"highest":[514],"increased":[515,587],"8":[522],"80-fold":[524],"decreased":[525],"IC50":[526],"control":[530],"or":[532],"wild-type":[536],"kinase.":[538,710],"created":[542],"protein":[545],"targeted":[546],"mitochondrial":[549,554,567,612],"matrix":[550],"fusing":[552],"targeting":[555],"signal":[556],"N-terminus":[559],"protein.":[562],"enzymatically":[570],"active":[571],"overexpression":[574],"TK\u203edeficient":[581],"resulted":[584,620],"such":[593],"1-\u03b2-D-arabinofuranosylthymine":[595],"(araT),":[596],"(E)-5-(2-bromovinyl)-2''-deoxyuridine":[597],"(BVDU),":[598],"5-bromo-2''":[599],"eoxyuridine":[600],"(5-Br-dUrd)":[601],"5fluoro2''deoxyuridine":[603],"(S-F-dUrd).":[604],"Labeling":[605],"using":[607],"[3H]-dThd":[608],"expression":[613,803],"nuclear":[618,643],"expression,":[619],"specific":[624],"[3H]-dTTP":[625,640],"activity":[626,679],"total":[629],"MP":[630],"pool":[631],"result":[635],"incorporation":[641,813],"into":[642,814],"DNA.":[644,815],"conducted":[646],"site-directed":[656],"A1a167":[659],"A1a168":[661],"A1a168His":[668],"A1a167Phe":[670],"turned":[672],"out":[673],"knocked-out":[676],"dThd":[678],"while":[680],"retaining":[681],"full":[682],"ability.":[685],"List":[686],"scientific":[688],"papers":[689],"I.":[690],"Solaroli":[691,722,765,793],"N,":[692,723,766,794],"Bjerke":[693],"M,":[694,698,768,796],"Amiri":[695],"MH,":[696],"Johansson":[697,767,795],"Karlsson":[699,729,771,799],"A":[700,730,772,800],"(2003).":[701],"Active":[702],"Eur":[711],"J":[712,758],"Biochem.":[713],"270(13):":[714],"2879-84.":[715],"https://pubmed.ncbi.nlm.nih.gov/12823558":[716],"II.":[717],"Balzarini":[718,769,797],"J,":[719,770,798],"Liekens":[720],"S,":[721],"El":[724],"Omari":[725],"K,":[726],"Stammers":[727],"DK,":[728],"(2006).":[731,773,801],"Engineering":[732],"single":[735],"conserved":[736],"residue":[739],"type":[744],"1":[745],"predominant":[750],"shift":[751],"phosphorylation.":[757],"Biol":[759],"Chem.":[760],"281(28):":[761],"19273-9.":[762],"https://pubmed.ncbi.nlm.nih.gov/16702226":[763],"III.":[764],"Enhanced":[774],"toxicity":[775],"mutants.":[787],"Gene":[788],"Ther.":[789],"[Accepted]":[790],"https://pubmed.ncbi.nlm.nih.gov/16885999":[791],"IV.":[792],"Mitochondrial":[802],"increase":[811],"[Manuscript]":[816]},"counts_by_year":[],"updated_date":"2026-09-11T09:22:31.653822","created_date":"2016-06-24T00:00:00"}
