{"id":"https://openalex.org/W2167608581","doi":"https://doi.org/10.1007/s00253-015-6610-5","title":"Development of a regulatable plasmid-based gene expression system for Clostridium thermocellum","display_name":"Development of a regulatable plasmid-based gene expression system for Clostridium thermocellum","publication_year":2015,"publication_date":"2015-05-20","ids":{"openalex":"https://openalex.org/W2167608581","doi":"https://doi.org/10.1007/s00253-015-6610-5","mag":"2167608581","pmid":"https://pubmed.ncbi.nlm.nih.gov/25994254"},"language":"en","primary_location":{"id":"doi:10.1007/s00253-015-6610-5","doi":"https://doi.org/10.1007/s00253-015-6610-5","is_oa":false,"landing_page_url":"https://doi.org/10.1007/s00253-015-6610-5","pdf_url":null,"source":{"id":"https://openalex.org/S70979998","display_name":"Applied Microbiology and Biotechnology","issn_l":"0175-7598","issn":["0175-7598","1432-0614"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","erih-plus","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied Microbiology and Biotechnology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5001459374","display_name":"Elizabeth B. Mearls","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I107672454","display_name":"Dartmouth College","ror":"https://ror.org/049s0rh22","country_code":"US","type":"education","lineage":["https://openalex.org/I107672454"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Elizabeth B. Mearls","raw_affiliation_strings":["Thayer School of Engineering, Dartmouth College, Hanover, NH, 03755, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Thayer School of Engineering, Dartmouth College, Hanover, NH, 03755, USA","institution_ids":["https://openalex.org/I107672454"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5009999197","display_name":"Daniel G. Olson","orcid":"https://orcid.org/0000-0001-5393-6302","observed_orcids":["https://orcid.org/0000-0001-5393-6302"]},"institutions":[{"id":"https://openalex.org/I107672454","display_name":"Dartmouth College","ror":"https://ror.org/049s0rh22","country_code":"US","type":"education","lineage":["https://openalex.org/I107672454"]},{"id":"https://openalex.org/I1289243028","display_name":"Oak Ridge National Laboratory","ror":"https://ror.org/01qz5mb56","country_code":"US","type":"facility","lineage":["https://openalex.org/I1289243028","https://openalex.org/I1330989302","https://openalex.org/I39565521","https://openalex.org/I4210159294"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Daniel G. Olson","raw_affiliation_strings":["BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA","Thayer School of Engineering, Dartmouth College, Hanover, NH, 03755, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA","institution_ids":["https://openalex.org/I1289243028"]},{"raw_affiliation_string":"Thayer School of Engineering, Dartmouth College, Hanover, NH, 03755, USA","institution_ids":["https://openalex.org/I107672454"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078427570","display_name":"Christopher D. Herring","orcid":"https://orcid.org/0000-0003-1621-5906","observed_orcids":["https://orcid.org/0000-0003-1621-5906"]},"institutions":[{"id":"https://openalex.org/I107672454","display_name":"Dartmouth College","ror":"https://ror.org/049s0rh22","country_code":"US","type":"education","lineage":["https://openalex.org/I107672454"]},{"id":"https://openalex.org/I4210097724","display_name":"Masco (United States)","ror":"https://ror.org/00xn3j339","country_code":"US","type":"company","lineage":["https://openalex.org/I4210097724"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Christopher D. Herring","raw_affiliation_strings":["Mascoma Corporation, 67 Etna Rd., Lebanon, NH, 03766, USA","Thayer School of Engineering, Dartmouth College, Hanover, NH, 03755, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Mascoma Corporation, 67 Etna Rd., Lebanon, NH, 03766, USA","institution_ids":["https://openalex.org/I4210097724"]},{"raw_affiliation_string":"Thayer School of Engineering, Dartmouth College, Hanover, NH, 03755, USA","institution_ids":["https://openalex.org/I107672454"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5046628962","display_name":"Lee Rybeck Lynd","orcid":"https://orcid.org/0000-0002-5642-668X","observed_orcids":["https://orcid.org/0000-0002-5642-668X"]},"institutions":[{"id":"https://openalex.org/I107672454","display_name":"Dartmouth College","ror":"https://ror.org/049s0rh22","country_code":"US","type":"education","lineage":["https://openalex.org/I107672454"]},{"id":"https://openalex.org/I1289243028","display_name":"Oak Ridge National Laboratory","ror":"https://ror.org/01qz5mb56","country_code":"US","type":"facility","lineage":["https://openalex.org/I1289243028","https://openalex.org/I1330989302","https://openalex.org/I39565521","https://openalex.org/I4210159294"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Lee R. Lynd","raw_affiliation_strings":["BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA","Thayer School of Engineering, Dartmouth College, Hanover, NH, 03755, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA","institution_ids":["https://openalex.org/I1289243028"]},{"raw_affiliation_string":"Thayer School of Engineering, Dartmouth College, Hanover, NH, 03755, USA","institution_ids":["https://openalex.org/I107672454"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5046628962"],"corresponding_institution_ids":["https://openalex.org/I107672454","https://openalex.org/I1289243028"],"apc_list":{"value":4190,"currency":"USD","value_usd":4190},"apc_paid":null,"fwci":0.8675,"has_fulltext":false,"cited_by_count":27,"citation_normalized_percentile":{"value":0.72611721,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":98},"biblio":{"volume":"99","issue":"18","first_page":"7589","last_page":"7599"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.7289999723434448,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.7289999723434448,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10171","display_name":"Biofuel production and bioconversion","score":0.20430000126361847,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.026900000870227814,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/clostridium-thermocellum","display_name":"Clostridium thermocellum","score":1.0},{"id":"https://openalex.org/keywords/inducible-gene-expression","display_name":"inducible gene expression","score":0.8040000200271606},{"id":"https://openalex.org/keywords/spo0a","display_name":"Spo0A","score":0.6610000133514404},{"id":"https://openalex.org/keywords/sporulation","display_name":"sporulation","score":0.7639999985694885},{"id":"https://openalex.org/keywords/carbon-catabolite-repression","display_name":"catabolite repression","score":0.8730000257492065},{"id":"https://openalex.org/keywords/cellobiose","display_name":"cellobiose","score":0.9710000157356262},{"id":"https://openalex.org/keywords/cellulose-solubilization","display_name":"cellulose solubilization","score":0.8429999947547913},{"id":"https://openalex.org/keywords/bioethanol-production","display_name":"bioethanol production","score":0.9010000228881836},{"id":"https://openalex.org/keywords/metabolic-engineering","display_name":"metabolic engineering","score":0.7710000276565552}],"concepts":[{"id":"https://openalex.org/C2776061948","wikidata":"https://www.wikidata.org/wiki/Q3681172","display_name":"Clostridium thermocellum","level":4,"score":0.9902697801589966},{"id":"https://openalex.org/C203075996","wikidata":"https://www.wikidata.org/wiki/Q139677","display_name":"Operon","level":4,"score":0.7736351490020752},{"id":"https://openalex.org/C83941689","wikidata":"https://www.wikidata.org/wiki/Q50350837","display_name":"Catabolite repression","level":4,"score":0.617572546005249},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6087557673454285},{"id":"https://openalex.org/C2776029434","wikidata":"https://www.wikidata.org/wiki/Q5058394","display_name":"Cellulosome","level":5,"score":0.5521782040596008},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5152733325958252},{"id":"https://openalex.org/C2225953","wikidata":"https://www.wikidata.org/wiki/Q1661899","display_name":"Inducer","level":3,"score":0.5093947052955627},{"id":"https://openalex.org/C2780412358","wikidata":"https://www.wikidata.org/wiki/Q409017","display_name":"Cellobiose","level":4,"score":0.4966815114021301},{"id":"https://openalex.org/C158448853","wikidata":"https://www.wikidata.org/wiki/Q425218","display_name":"Repressor","level":4,"score":0.46690067648887634},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.4613080322742462},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4206821024417877},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.40783265233039856},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.11727434396743774},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.09651634097099304},{"id":"https://openalex.org/C141603559","wikidata":"https://www.wikidata.org/wiki/Q410324","display_name":"Cellulase","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D004187","descriptor_name":"Disaccharides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008967","descriptor_name":"Molecular Biology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D011401","descriptor_name":"Promoter Regions, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012097","descriptor_name":"Repressor Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012097","descriptor_name":"Repressor Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015533","descriptor_name":"Transcriptional Activation","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D015964","descriptor_name":"Gene Expression Regulation, Bacterial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D048013","descriptor_name":"Acetivibrio thermocellus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1007/s00253-015-6610-5","doi":"https://doi.org/10.1007/s00253-015-6610-5","is_oa":false,"landing_page_url":"https://doi.org/10.1007/s00253-015-6610-5","pdf_url":null,"source":{"id":"https://openalex.org/S70979998","display_name":"Applied Microbiology and Biotechnology","issn_l":"0175-7598","issn":["0175-7598","1432-0614"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","erih-plus","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied Microbiology and Biotechnology","raw_type":"journal-article"},{"id":"pmid:25994254","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/25994254","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied microbiology and biotechnology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.5690000057220459,"id":"https://metadata.un.org/sdg/7","display_name":"Affordable and clean energy"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320306084","display_name":"U.S. Department of Energy","ror":"https://ror.org/01bj3aw27"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":51,"referenced_works":["https://openalex.org/W70864286","https://openalex.org/W569215458","https://openalex.org/W1516985353","https://openalex.org/W1593379289","https://openalex.org/W1917794312","https://openalex.org/W1975877547","https://openalex.org/W1978831120","https://openalex.org/W1992644222","https://openalex.org/W1994089750","https://openalex.org/W1999046178","https://openalex.org/W1999251928","https://openalex.org/W2001373309","https://openalex.org/W2002582075","https://openalex.org/W2004801113","https://openalex.org/W2006495190","https://openalex.org/W2022064097","https://openalex.org/W2029316591","https://openalex.org/W2033727002","https://openalex.org/W2055749561","https://openalex.org/W2056463915","https://openalex.org/W2065708815","https://openalex.org/W2084757245","https://openalex.org/W2090591880","https://openalex.org/W2096203216","https://openalex.org/W2098839738","https://openalex.org/W2099047627","https://openalex.org/W2099728864","https://openalex.org/W2099865775","https://openalex.org/W2100067322","https://openalex.org/W2104494486","https://openalex.org/W2113496638","https://openalex.org/W2120580364","https://openalex.org/W2124830355","https://openalex.org/W2129739953","https://openalex.org/W2131923812","https://openalex.org/W2135066331","https://openalex.org/W2135352791","https://openalex.org/W2135921763","https://openalex.org/W2137785484","https://openalex.org/W2142353458","https://openalex.org/W2143614758","https://openalex.org/W2146505082","https://openalex.org/W2148641266","https://openalex.org/W2159180716","https://openalex.org/W2164264914","https://openalex.org/W2164386192","https://openalex.org/W2169889515","https://openalex.org/W2337925232","https://openalex.org/W2987253468","https://openalex.org/W4233646211","https://openalex.org/W4242441490"],"related_works":["https://openalex.org/W2129739953","https://openalex.org/W2587037330","https://openalex.org/W4300446835","https://openalex.org/W2564299742","https://openalex.org/W4414412261","https://openalex.org/W6977147504","https://openalex.org/W2164386192","https://openalex.org/W2593839367","https://openalex.org/W2137539310","https://openalex.org/W2113050393"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":5},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":3},{"year":2016,"cited_by_count":3}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}